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The map label for this gene is truD

Identifier: 18977927

GI number: 18977927

Start: 1451335

End: 1452558

Strand: Reverse

Name: truD

Synonym: PF1555

Alternate gene names: 18977927

Gene position: 1452558-1451335 (Counterclockwise)

Preceding gene: 18977928

Following gene: 18977923

Centisome position: 76.12

GC content: 38.56

Gene sequence:

>1224_bases
ATGAGTGATCCTGTTGGAGGGCTGAAGTACTTGACAACTTCTCCTGGGATAGGTGGGAAAATTAAGGTATATCCCGAAGA
TTTCATAGTTAAAGAGGTTATTCCAAAGTCCATTTTTAAAGCTGGGAAATGCAAAATATATATTTTAAAGAAGAAAAATT
GGGAAACCATGGCCGCAATAAAGGAGATTGCAAAACGAGTAGGAGTTCATTATTCGGAAATTGGCTTTGCAGGAACCAAA
GATCGACATGCTGTGACTTACCAGTATATTAGCATTTGTAGAGATGTTAACTTGGAAGAGGTGAAAATTAGAGATATTGA
GCTCAAGTTCGTTGGATATGGAAGACCTCTAAAGCTTGGCTTTCTCCTGGGGAACTTCTTTAAGATTAGGGTTAGAGAAT
CCAATCCCTCACTGTTGCCAAGTGTAATTGAAGAAGCCAAAGAAAAGGGAGGCTTTCCAAATTATTTTGGCATACAAAGG
TTTGGTGAAAAGAGGAGTGTAAATCATGTTGTTGGCAAGCTACTTCTCTTGGGAAAATATGAAGAGGCTGCGGAAGTATT
TTTGGGATTCCCAGGAAAAGGAATGGAAGGAGATGAAGCTAGAAGAAAATTTCTGGAGACTAAGGATGTTGATCTTGCTC
TTAGGGAGTTTCCAAACTTCTTGAGATATGAGAGAGCAATGCTCTACAGATACAAAGAAACAGGTAGCTGGAAGAAAGCA
TTTTTAGTTCTTCCTCGACCGATACTGAGGATATTTATTCACTCCTTCCAGTCCTATCTCTTTAACCTTTATCTTTCTAG
GAGAATTGAAGAGGGACTTCCTTTAAATAAAGCTCTCCCAGGTGATATAGTTATTCAAGTAAAGAAAGGCATACCCTTGA
GAAAGAGAACGTATAGAGTCACCGAGAATAACGTTGATTTTGTTAATAGAAAAATTAAAGAAGGTGAGGCGATGGTTTCT
GGCCCTATTTTTGGCTATATGTACAGAAAAGGAAAGGGAATTCCTGGAAGATTGGAGGAAGAGATTTTAGAAGAAGCGGG
AGTCAAACTTGAATACTTTAAGAAGCTTCCAAAACCAATGAGGGAGCCTGGGGGAAGAAGGGAATTACTGATAAGGCCAA
TGAAATTTGCATACAAAGTTGAGGATAGGGATGTTATCTTTAGGTTTTTCCTTCCTAAAGGAGTTTACGCAACAAGCGTT
TTAAGGGAGATAATGAAAAACTAA

Upstream 100 bases:

>100_bases
ATTCCTCCAGGAACAATAACAGTTTTAGCCATAGGGCCTGGGCCAGAGGAAGTTGTAGATAAGGTGACAGGACATTTAAA
ACTTCTATAGGTAGAAGAGT

Downstream 100 bases:

>100_bases
TCCAGTTCTATTCCCTTAACCTTGTCACTCACATAACCCTCCAGGATCTCAACTTTTACCTCTCTTTTCTCTCCTGTCAA
ATCGGCCATTAAACTTACAG

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSDPVGGLKYLTTSPGIGGKIKVYPEDFIVKEVIPKSIFKAGKCKIYILKKKNWETMAAIKEIAKRVGVHYSEIGFAGTK
DRHAVTYQYISICRDVNLEEVKIRDIELKFVGYGRPLKLGFLLGNFFKIRVRESNPSLLPSVIEEAKEKGGFPNYFGIQR
FGEKRSVNHVVGKLLLLGKYEEAAEVFLGFPGKGMEGDEARRKFLETKDVDLALREFPNFLRYERAMLYRYKETGSWKKA
FLVLPRPILRIFIHSFQSYLFNLYLSRRIEEGLPLNKALPGDIVIQVKKGIPLRKRTYRVTENNVDFVNRKIKEGEAMVS
GPIFGYMYRKGKGIPGRLEEEILEEAGVKLEYFKKLPKPMREPGGRRELLIRPMKFAYKVEDRDVIFRFFLPKGVYATSV
LREIMKN

Sequences:

>Translated_407_residues
MSDPVGGLKYLTTSPGIGGKIKVYPEDFIVKEVIPKSIFKAGKCKIYILKKKNWETMAAIKEIAKRVGVHYSEIGFAGTK
DRHAVTYQYISICRDVNLEEVKIRDIELKFVGYGRPLKLGFLLGNFFKIRVRESNPSLLPSVIEEAKEKGGFPNYFGIQR
FGEKRSVNHVVGKLLLLGKYEEAAEVFLGFPGKGMEGDEARRKFLETKDVDLALREFPNFLRYERAMLYRYKETGSWKKA
FLVLPRPILRIFIHSFQSYLFNLYLSRRIEEGLPLNKALPGDIVIQVKKGIPLRKRTYRVTENNVDFVNRKIKEGEAMVS
GPIFGYMYRKGKGIPGRLEEEILEEAGVKLEYFKKLPKPMREPGGRRELLIRPMKFAYKVEDRDVIFRFFLPKGVYATSV
LREIMKN
>Mature_406_residues
SDPVGGLKYLTTSPGIGGKIKVYPEDFIVKEVIPKSIFKAGKCKIYILKKKNWETMAAIKEIAKRVGVHYSEIGFAGTKD
RHAVTYQYISICRDVNLEEVKIRDIELKFVGYGRPLKLGFLLGNFFKIRVRESNPSLLPSVIEEAKEKGGFPNYFGIQRF
GEKRSVNHVVGKLLLLGKYEEAAEVFLGFPGKGMEGDEARRKFLETKDVDLALREFPNFLRYERAMLYRYKETGSWKKAF
LVLPRPILRIFIHSFQSYLFNLYLSRRIEEGLPLNKALPGDIVIQVKKGIPLRKRTYRVTENNVDFVNRKIKEGEAMVSG
PIFGYMYRKGKGIPGRLEEEILEEAGVKLEYFKKLPKPMREPGGRRELLIRPMKFAYKVEDRDVIFRFFLPKGVYATSVL
REIMKN

Specific function: Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI148762985, Length=410, Percent_Identity=29.2682926829268, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148762961, Length=410, Percent_Identity=29.2682926829268, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148762948, Length=410, Percent_Identity=29.2682926829268, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI50727002, Length=447, Percent_Identity=27.5167785234899, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1789102, Length=163, Percent_Identity=34.3558282208589, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557232, Length=245, Percent_Identity=28.9795918367347, Blast_Score=82, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324817, Length=446, Percent_Identity=27.3542600896861, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21355799, Length=295, Percent_Identity=28.4745762711864, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_PYRFU (Q8U0N1)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579284.1
- ProteinModelPortal:   Q8U0N1
- EnsemblBacteria:   EBPYRT00000004396
- GeneID:   1469431
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1555
- NMPDR:   fig|186497.1.peg.1602
- GeneTree:   EBGT00050000023332
- HOGENOM:   HBG541221
- OMA:   WAGTKDK
- ProtClustDB:   PRK00984
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 46977; Mature: 46846

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPVGGLKYLTTSPGIGGKIKVYPEDFIVKEVIPKSIFKAGKCKIYILKKKNWETMAAI
CCCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
KEIAKRVGVHYSEIGFAGTKDRHAVTYQYISICRDVNLEEVKIRDIELKFVGYGRPLKLG
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCEEHH
FLLGNFFKIRVRESNPSLLPSVIEEAKEKGGFPNYFGIQRFGEKRSVNHVVGKLLLLGKY
EECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
EEAAEVFLGFPGKGMEGDEARRKFLETKDVDLALREFPNFLRYERAMLYRYKETGSWKKA
HHHHHHEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCEE
FLVLPRPILRIFIHSFQSYLFNLYLSRRIEEGLPLNKALPGDIVIQVKKGIPLRKRTYRV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHEEEE
TENNVDFVNRKIKEGEAMVSGPIFGYMYRKGKGIPGRLEEEILEEAGVKLEYFKKLPKPM
CCCCHHHHHHHHHCCCEEECCCHHEEHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCHHH
REPGGRRELLIRPMKFAYKVEDRDVIFRFFLPKGVYATSVLREIMKN
HCCCCCHHEEEECCHHEEEECCCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SDPVGGLKYLTTSPGIGGKIKVYPEDFIVKEVIPKSIFKAGKCKIYILKKKNWETMAAI
CCCCCCCEEEECCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
KEIAKRVGVHYSEIGFAGTKDRHAVTYQYISICRDVNLEEVKIRDIELKFVGYGRPLKLG
HHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEEEEEECCCCEEHH
FLLGNFFKIRVRESNPSLLPSVIEEAKEKGGFPNYFGIQRFGEKRSVNHVVGKLLLLGKY
EECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCC
EEAAEVFLGFPGKGMEGDEARRKFLETKDVDLALREFPNFLRYERAMLYRYKETGSWKKA
HHHHHHEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCCCEE
FLVLPRPILRIFIHSFQSYLFNLYLSRRIEEGLPLNKALPGDIVIQVKKGIPLRKRTYRV
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCHHEEEE
TENNVDFVNRKIKEGEAMVSGPIFGYMYRKGKGIPGRLEEEILEEAGVKLEYFKKLPKPM
CCCCHHHHHHHHHCCCEEECCCHHEEHHHCCCCCCCHHHHHHHHHCCCCHHHHHHCCHHH
REPGGRRELLIRPMKFAYKVEDRDVIFRFFLPKGVYATSVLREIMKN
HCCCCCHHEEEECCHHEEEECCCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA