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The map label for this gene is rsmF [H]

Identifier: 18977925

GI number: 18977925

Start: 1450145

End: 1451080

Strand: Direct

Name: rsmF [H]

Synonym: PF1553

Alternate gene names: 18977925

Gene position: 1450145-1451080 (Clockwise)

Preceding gene: 18977924

Following gene: 18977926

Centisome position: 75.99

GC content: 40.17

Gene sequence:

>936_bases
GTGGAGTATTTAGAAGAATTCAGAAAAATAAACGAAAAATTAGTGGAGAGATACTCAAAACTTGACGAAAGCGAAGACTT
TTGGAAGTATCTGTATAAACCCCTCCGCCCTAGTATAAGGATAAACACGTTGAAAGGCCATCTAAACGAGATTAAAAGCT
TACTAGAAGAGAAGTTTGAACTAGAACCAATACCATGGACTAAAGGAGAGGGGTTCTTCATAAAGAGATATGAAGTAAAC
TTCGGAGAGCTAATTGAATACTCGCTGGGCCTTATAATTCCCCAGGAAGCAAGTTCAATGATACCTCCCGTCGTCCTAGA
TCCACAACCTGGAGAATTAGTTCTAGATATGGCTGCAGCACCTGGGAGCAAAACAACCCAGATAGCCCAGTACATGAACA
ATGAAGGTTGTATAATTGCAAATGATGCAAAAAGAGACAGGGCAAACATACTGATATCAAACCTCAATAAGGCTGGAGTT
CTAATTGCAAAAGTGACTGTCAAAGATGGAGCATACTTTAGAAGATATGAAAACACATTTGATAAAATCTTATTAGATGC
TCCATGCTCCTCCGTTGGGATGATAAGGAAGAACTTCAAGTTTGCACGAACTTGGAGCATGGGGAAAGTTTACTATCACT
CAAAGCTCCAAAAGAGACTAATACTTGCGGCATACAAAGCTCTAAAGCCGGGGGGAGTCTTAGTGTACTCCACATGTACA
GTGGATCCTCTAGAAAACGAGGAAGTGGTGGATTTCTTGCTCCAGAAAACTGACGCAAAACTTGAGAAGATTAACCTCCC
ATTAAAATCAACTGAACCGGTGTTGGAGTGGGAGGGCAAAAAGTATTCTGATGAACTTAAGAAGACCCTTAGAATACATC
CCCAAGATAATGATACGGAAGCGTTTTATATTGCAAAGATAAGAAAACCTAGGTGA

Upstream 100 bases:

>100_bases
GAAGTCAAGGCAATAACATATCACGACATGAAAATAGAGAGACTACCAGATGGAAGATGGATGGCTCAGCTAGTTCCAGA
CATATGATGGAGGTGTAAAA

Downstream 100 bases:

>100_bases
ATGATTATGCTTGTAAAGAAGAAGGGTGTATTCACTGAACTTGACGCTCAAAAAGTAATAGAAATGGCAAGTAAAAGCAT
GGAAAAAGAAATAATTGTCA

Product: nol1-nop2-sun family nucleolar protein I

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MEYLEEFRKINEKLVERYSKLDESEDFWKYLYKPLRPSIRINTLKGHLNEIKSLLEEKFELEPIPWTKGEGFFIKRYEVN
FGELIEYSLGLIIPQEASSMIPPVVLDPQPGELVLDMAAAPGSKTTQIAQYMNNEGCIIANDAKRDRANILISNLNKAGV
LIAKVTVKDGAYFRRYENTFDKILLDAPCSSVGMIRKNFKFARTWSMGKVYYHSKLQKRLILAAYKALKPGGVLVYSTCT
VDPLENEEVVDFLLQKTDAKLEKINLPLKSTEPVLEWEGKKYSDELKKTLRIHPQDNDTEAFYIAKIRKPR

Sequences:

>Translated_311_residues
MEYLEEFRKINEKLVERYSKLDESEDFWKYLYKPLRPSIRINTLKGHLNEIKSLLEEKFELEPIPWTKGEGFFIKRYEVN
FGELIEYSLGLIIPQEASSMIPPVVLDPQPGELVLDMAAAPGSKTTQIAQYMNNEGCIIANDAKRDRANILISNLNKAGV
LIAKVTVKDGAYFRRYENTFDKILLDAPCSSVGMIRKNFKFARTWSMGKVYYHSKLQKRLILAAYKALKPGGVLVYSTCT
VDPLENEEVVDFLLQKTDAKLEKINLPLKSTEPVLEWEGKKYSDELKKTLRIHPQDNDTEAFYIAKIRKPR
>Mature_311_residues
MEYLEEFRKINEKLVERYSKLDESEDFWKYLYKPLRPSIRINTLKGHLNEIKSLLEEKFELEPIPWTKGEGFFIKRYEVN
FGELIEYSLGLIIPQEASSMIPPVVLDPQPGELVLDMAAAPGSKTTQIAQYMNNEGCIIANDAKRDRANILISNLNKAGV
LIAKVTVKDGAYFRRYENTFDKILLDAPCSSVGMIRKNFKFARTWSMGKVYYHSKLQKRLILAAYKALKPGGVLVYSTCT
VDPLENEEVVDFLLQKTDAKLEKINLPLKSTEPVLEWEGKKYSDELKKTLRIHPQDNDTEAFYIAKIRKPR

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=281, Percent_Identity=35.5871886120996, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI76150623, Length=281, Percent_Identity=35.5871886120996, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI39995082, Length=364, Percent_Identity=28.2967032967033, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI301336155, Length=344, Percent_Identity=28.4883720930233, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI32698918, Length=226, Percent_Identity=37.6106194690265, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI270288818, Length=335, Percent_Identity=25.3731343283582, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI23199998, Length=327, Percent_Identity=25.6880733944954, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI270288816, Length=327, Percent_Identity=25.6880733944954, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI8922322, Length=327, Percent_Identity=25.6880733944954, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI11545785, Length=168, Percent_Identity=33.9285714285714, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI40316918, Length=169, Percent_Identity=34.9112426035503, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI87081985, Length=292, Percent_Identity=39.7260273972603, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI2367212, Length=183, Percent_Identity=38.2513661202186, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17536757, Length=282, Percent_Identity=36.8794326241135, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI25143471, Length=276, Percent_Identity=32.2463768115942, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI115534021, Length=231, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71998419, Length=231, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=306, Percent_Identity=37.2549019607843, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319447, Length=389, Percent_Identity=29.8200514138817, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI22024126, Length=285, Percent_Identity=35.7894736842105, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI21355201, Length=284, Percent_Identity=30.9859154929577, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21356513, Length=240, Percent_Identity=34.1666666666667, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24668781, Length=240, Percent_Identity=34.1666666666667, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19920494, Length=175, Percent_Identity=31.4285714285714, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356579, Length=225, Percent_Identity=28.4444444444444, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 35851; Mature: 35851

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYLEEFRKINEKLVERYSKLDESEDFWKYLYKPLRPSIRINTLKGHLNEIKSLLEEKFE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHC
LEPIPWTKGEGFFIKRYEVNFGELIEYSLGLIIPQEASSMIPPVVLDPQPGELVLDMAAA
CCCCCCCCCCCEEEEEEECCHHHHHHHHHCEEECCCHHHCCCCEEECCCCCCEEEEEECC
PGSKTTQIAQYMNNEGCIIANDAKRDRANILISNLNKAGVLIAKVTVKDGAYFRRYENTF
CCCCHHHHHHHHCCCCEEEECCCCCCCHHEEEECCCCCCEEEEEEEECCCHHHHHHHHHH
DKILLDAPCSSVGMIRKNFKFARTWSMGKVYYHSKLQKRLILAAYKALKPGGVLVYSTCT
HHEEECCCCCHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
VDPLENEEVVDFLLQKTDAKLEKINLPLKSTEPVLEWEGKKYSDELKKTLRIHPQDNDTE
CCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEECCCCHHHHHHHHEEECCCCCCCC
AFYIAKIRKPR
EEEEEEECCCC
>Mature Secondary Structure
MEYLEEFRKINEKLVERYSKLDESEDFWKYLYKPLRPSIRINTLKGHLNEIKSLLEEKFE
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHC
LEPIPWTKGEGFFIKRYEVNFGELIEYSLGLIIPQEASSMIPPVVLDPQPGELVLDMAAA
CCCCCCCCCCCEEEEEEECCHHHHHHHHHCEEECCCHHHCCCCEEECCCCCCEEEEEECC
PGSKTTQIAQYMNNEGCIIANDAKRDRANILISNLNKAGVLIAKVTVKDGAYFRRYENTF
CCCCHHHHHHHHCCCCEEEECCCCCCCHHEEEECCCCCCEEEEEEEECCCHHHHHHHHHH
DKILLDAPCSSVGMIRKNFKFARTWSMGKVYYHSKLQKRLILAAYKALKPGGVLVYSTCT
HHEEECCCCCHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
VDPLENEEVVDFLLQKTDAKLEKINLPLKSTEPVLEWEGKKYSDELKKTLRIHPQDNDTE
CCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCEEECCCCHHHHHHHHEEECCCCCCCC
AFYIAKIRKPR
EEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA