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The map label for this gene is thiP [H]

Identifier: 18977892

GI number: 18977892

Start: 1418206

End: 1419771

Strand: Reverse

Name: thiP [H]

Synonym: PF1520

Alternate gene names: 18977892

Gene position: 1419771-1418206 (Counterclockwise)

Preceding gene: 18977893

Following gene: 18977891

Centisome position: 74.4

GC content: 41.7

Gene sequence:

>1566_bases
ATGAAGAGATTGTTGCCTTTATCACTTTTGTTCTTGGGGGTGTTTTTCTACCTTCCAATTTTCCTTATAGTCAAGGAGGG
AATCGATGTAAAGGCCTTAGTGGATGTGGTGACTGATAGTTATCACTTAAGAGTTATTTCCTTTACATTTGCCCAGGCAA
TAGGTTCAACTGTGGTAACTCTTCTCATAGGGCTTCCTGGGGCCTACATCTTTGCTAAGTATGACTTCCCGGGGAAAGAT
TTTCTTAAGGCTTTAATCACCATCCCCTTTGTAATGCCAAGCGTCATGGTTGCTTTAGGATTTCTAATACTTTTTGGAAA
GTCTGGGCCCCTTGGGTGGCTTAATGTTCTGTACTCATGGAAGGCGATTGTTCTAGCTCATGCCTTCTACAATTATCCAG
TGGTAGTGAGAATGGTGGGTACTGTACTGGAAAGAACAAATCCCCATTATGAGGAAGCGGCAATGACGTTAGGGGCCAAG
GGTTTTTTCCTCTTTAGGAAGGTGACGTTCCCTCTAATTCTTCCAGGAGTTATATCTTCTGCCCTTCTAACATTTACATT
CTCCTTCATGAGCTTTTCAATTCCTCTAATCTTGGGTGGGTATAGGTACTCAACCCTTGAAGTTGATATCTTTACCTCAA
TAATGGTTCTTTTGGACTTCAAAACAGGCTCCGCGTTAGCTCTTCTTCAACTTACTCTAAGCTTAACCTTTATGTACGCC
TACTTAAAGGTGCTTAGCATTTATGCAAAAATGGAAGAGCAGAGAATAATAATAGGGAAGAAGAAATTGAGAGGGTTGTC
TCTCTACTTAGCTCTCCTCTATTTTGTGGTCATTGGAGTATTTATTATTGGCCCACTGATAGCTGTTGTTATTGACTCTC
TAACTTATGACGGGAGGATTAGTTTGGAATGGTATATGAGGGCGTTTTCGGAAGAATACAATCCAATGTTTGGAACAACG
TCTCTAAGGACAATTCTTAACTCTCTAATGTTCGGTGTCTTCACCATGATAATTTCTCTTTTAGTTGCTCTTCCTCTGGC
CTATACACTAACTAAATACAGATTCAGAGGAAAAATCTTGGTAGATGCCCTTGCAACTCTTCCCTTGGCTAGCTCCTCTG
TAGTTCTGGGCCTTGGATATCTCCTCGCCTTTAGGAAGACTCCTCTCTATGACTCCTGGGTAATAATTGCGTTGGCTCAC
TCAACGGTTGCATATCCCTTTGTGCTAAGAGCTCTTTCGTCCTCTCTAGTGAAGGTAAAGAAATCTCTGTTTGAAGCTGC
ATTAACACTGGGGGCTAAGGAAGGGATTGCCTTTAAAGAGGTAGAGCTTCCTCTAATACAAAAGGGTCTCATAACTGGGG
CACTATTTGCCTTTGCAATAAGCTTGGGAGAGCTCGCAACTACTTACATGCTTGCAAGGCCAGAATACACAACACTAACG
ATGGCCATCTATAAGTTTATATCCGCAAGGCAATTTGGCCCAGCTTCAGCTTTGGCTGTAATCCTAATGCTTGTTTCTGG
AATTAGCTTTTTGATAATTGAAAAAGTTGGTGGTGAGATATGGTAA

Upstream 100 bases:

>100_bases
TTAACCAACGATGCGTTAAATAAAGCAGGAACTCCAGAGCTCAAGTTTTCGGCAGCAAGAATAAGGAAAGTTTCATAACT
CCTTAATCTAACAAACAATC

Downstream 100 bases:

>100_bases
CGGTAGAACTCGAGAATGTAACTAAATCTTTCGAGAACTTTAGGCTTGAGGTGAGCTTAAAAGTTAAAGACGGCGAGCTG
TTAACTCTCTTAGGTCCAAG

Product: ABC transporter integral membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MKRLLPLSLLFLGVFFYLPIFLIVKEGIDVKALVDVVTDSYHLRVISFTFAQAIGSTVVTLLIGLPGAYIFAKYDFPGKD
FLKALITIPFVMPSVMVALGFLILFGKSGPLGWLNVLYSWKAIVLAHAFYNYPVVVRMVGTVLERTNPHYEEAAMTLGAK
GFFLFRKVTFPLILPGVISSALLTFTFSFMSFSIPLILGGYRYSTLEVDIFTSIMVLLDFKTGSALALLQLTLSLTFMYA
YLKVLSIYAKMEEQRIIIGKKKLRGLSLYLALLYFVVIGVFIIGPLIAVVIDSLTYDGRISLEWYMRAFSEEYNPMFGTT
SLRTILNSLMFGVFTMIISLLVALPLAYTLTKYRFRGKILVDALATLPLASSSVVLGLGYLLAFRKTPLYDSWVIIALAH
STVAYPFVLRALSSSLVKVKKSLFEAALTLGAKEGIAFKEVELPLIQKGLITGALFAFAISLGELATTYMLARPEYTTLT
MAIYKFISARQFGPASALAVILMLVSGISFLIIEKVGGEIW

Sequences:

>Translated_521_residues
MKRLLPLSLLFLGVFFYLPIFLIVKEGIDVKALVDVVTDSYHLRVISFTFAQAIGSTVVTLLIGLPGAYIFAKYDFPGKD
FLKALITIPFVMPSVMVALGFLILFGKSGPLGWLNVLYSWKAIVLAHAFYNYPVVVRMVGTVLERTNPHYEEAAMTLGAK
GFFLFRKVTFPLILPGVISSALLTFTFSFMSFSIPLILGGYRYSTLEVDIFTSIMVLLDFKTGSALALLQLTLSLTFMYA
YLKVLSIYAKMEEQRIIIGKKKLRGLSLYLALLYFVVIGVFIIGPLIAVVIDSLTYDGRISLEWYMRAFSEEYNPMFGTT
SLRTILNSLMFGVFTMIISLLVALPLAYTLTKYRFRGKILVDALATLPLASSSVVLGLGYLLAFRKTPLYDSWVIIALAH
STVAYPFVLRALSSSLVKVKKSLFEAALTLGAKEGIAFKEVELPLIQKGLITGALFAFAISLGELATTYMLARPEYTTLT
MAIYKFISARQFGPASALAVILMLVSGISFLIIEKVGGEIW
>Mature_521_residues
MKRLLPLSLLFLGVFFYLPIFLIVKEGIDVKALVDVVTDSYHLRVISFTFAQAIGSTVVTLLIGLPGAYIFAKYDFPGKD
FLKALITIPFVMPSVMVALGFLILFGKSGPLGWLNVLYSWKAIVLAHAFYNYPVVVRMVGTVLERTNPHYEEAAMTLGAK
GFFLFRKVTFPLILPGVISSALLTFTFSFMSFSIPLILGGYRYSTLEVDIFTSIMVLLDFKTGSALALLQLTLSLTFMYA
YLKVLSIYAKMEEQRIIIGKKKLRGLSLYLALLYFVVIGVFIIGPLIAVVIDSLTYDGRISLEWYMRAFSEEYNPMFGTT
SLRTILNSLMFGVFTMIISLLVALPLAYTLTKYRFRGKILVDALATLPLASSSVVLGLGYLLAFRKTPLYDSWVIIALAH
STVAYPFVLRALSSSLVKVKKSLFEAALTLGAKEGIAFKEVELPLIQKGLITGALFAFAISLGELATTYMLARPEYTTLT
MAIYKFISARQFGPASALAVILMLVSGISFLIIEKVGGEIW

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=204, Percent_Identity=34.3137254901961, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786254, Length=507, Percent_Identity=23.8658777120316, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082099, Length=232, Percent_Identity=31.8965517241379, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1787368, Length=210, Percent_Identity=25.7142857142857, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786980, Length=132, Percent_Identity=34.8484848484849, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57668; Mature: 57668

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50928 ABC_TM1 ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLPLSLLFLGVFFYLPIFLIVKEGIDVKALVDVVTDSYHLRVISFTFAQAIGSTVVT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
LLIGLPGAYIFAKYDFPGKDFLKALITIPFVMPSVMVALGFLILFGKSGPLGWLNVLYSW
HHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KAIVLAHAFYNYPVVVRMVGTVLERTNPHYEEAAMTLGAKGFFLFRKVTFPLILPGVISS
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ALLTFTFSFMSFSIPLILGGYRYSTLEVDIFTSIMVLLDFKTGSALALLQLTLSLTFMYA
HHHHHHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YLKVLSIYAKMEEQRIIIGKKKLRGLSLYLALLYFVVIGVFIIGPLIAVVIDSLTYDGRI
HHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
SLEWYMRAFSEEYNPMFGTTSLRTILNSLMFGVFTMIISLLVALPLAYTLTKYRFRGKIL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VDALATLPLASSSVVLGLGYLLAFRKTPLYDSWVIIALAHSTVAYPFVLRALSSSLVKVK
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
KSLFEAALTLGAKEGIAFKEVELPLIQKGLITGALFAFAISLGELATTYMLARPEYTTLT
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHH
MAIYKFISARQFGPASALAVILMLVSGISFLIIEKVGGEIW
HHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRLLPLSLLFLGVFFYLPIFLIVKEGIDVKALVDVVTDSYHLRVISFTFAQAIGSTVVT
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
LLIGLPGAYIFAKYDFPGKDFLKALITIPFVMPSVMVALGFLILFGKSGPLGWLNVLYSW
HHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KAIVLAHAFYNYPVVVRMVGTVLERTNPHYEEAAMTLGAKGFFLFRKVTFPLILPGVISS
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ALLTFTFSFMSFSIPLILGGYRYSTLEVDIFTSIMVLLDFKTGSALALLQLTLSLTFMYA
HHHHHHHHHHHHHHHHEECCCEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YLKVLSIYAKMEEQRIIIGKKKLRGLSLYLALLYFVVIGVFIIGPLIAVVIDSLTYDGRI
HHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
SLEWYMRAFSEEYNPMFGTTSLRTILNSLMFGVFTMIISLLVALPLAYTLTKYRFRGKIL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VDALATLPLASSSVVLGLGYLLAFRKTPLYDSWVIIALAHSTVAYPFVLRALSSSLVKVK
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
KSLFEAALTLGAKEGIAFKEVELPLIQKGLITGALFAFAISLGELATTYMLARPEYTTLT
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHH
MAIYKFISARQFGPASALAVILMLVSGISFLIIEKVGGEIW
HHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]