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The map label for this gene is purP

Identifier: 18977889

GI number: 18977889

Start: 1415074

End: 1416078

Strand: Reverse

Name: purP

Synonym: PF1517

Alternate gene names: 18977889

Gene position: 1416078-1415074 (Counterclockwise)

Preceding gene: 18977891

Following gene: 18977888

Centisome position: 74.21

GC content: 41.19

Gene sequence:

>1005_bases
ATGAAGGTTAGGATAGCAACTTATGCCTCCCATTCAGCCCTTCAAATACTCAAGGGGGCAAAAGATGAAGGGTTTGAGAC
AATAGCATTTGGTTCCTCAAAGGTGAAGCCTCTCTATACCAAATACTTCCCAGTAGCTGATTATTTCATCGAAGAAAAGT
ATCCAGAAGAAGAACTACTAAATTTGAATGCTGTAGTAGTTCCAACAGGTTCTTTTGTTGCTCACCTGGGAATAGAGCTT
GTTGAAAACATGAAAGTTCCCTATTTCGGAAATAAAAGAGTTCTTAGGTGGGAGAGTGACAGAAATTTAGAGAGAAAATG
GCTCAAAAAAGCAGGAATAAGAGTTCCAGAAGTTTATGAAGATCCTGACGATATAGAGAAACCAGTGATTGTAAAGCCAC
ATGGAGCTAAAGGAGGGAAGGGGTACTTCTTAGCTAAAGATCCAGAAGATTTCTGGAGAAAGGCTGAGAAATTCCTTGGG
ATTAAAAGGAAGGAAGATTTAAAGAACATTCAAATCCAGGAATACGTGTTGGGCGTCCCAGTATATCCCCATTACTTCTA
CTCCAAAGTTAGAGAGGAGTTGGAGCTAATGAGCATAGATAGGAGGTATGAGAGCAACGTTGACGCAATAGGAAGAATAC
CAGCAAAAGATCAGCTTGAATTTGATATGGATATCACCTATACGGTCATAGGAAACATTCCAATAGTCTTAAGAGAGAGC
CTTTTAATGGACGTGATAGAGGCAGGAGAGAGAGTTGTAAAGGCTGCGGAGGAGCTTATGGGAGGGCTATGGGGCCCGTT
CTGTCTCGAGGGGGTATTTACACCGGATCTTGAATTTGTTGTCTTTGAAATATCAGCAAGAATAGTGGCCGGAACTAACA
TCTTTGTTAACGGCTCTCCATACACATGGCTTAGATATGATAGGCCAGTAAGTACTGGAAGAAGGATAGCCATGGAAATT
AGAGAGGCAATTGAGAATGATATGCTGGAGAAAGTTCTTACCTAA

Upstream 100 bases:

>100_bases
CCTTAAACTTTTCTGGTTCGAAGTTTAAGCTTTTTATTTACATAAAAATGTCAAAAAAATCTTTAAAAAACTCGAATTTT
TACATAAAAATGGTGAAAAG

Downstream 100 bases:

>100_bases
TCCTTATAATCTATTTTATCTAAAAATGTCCAGAGGGGAATTTGTTTACAACTTTATGTTTAATTTCGCAAGACTTTTAA
TAAAGTTTAGACAATCGTTT

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKVRIATYASHSALQILKGAKDEGFETIAFGSSKVKPLYTKYFPVADYFIEEKYPEEELLNLNAVVVPTGSFVAHLGIEL
VENMKVPYFGNKRVLRWESDRNLERKWLKKAGIRVPEVYEDPDDIEKPVIVKPHGAKGGKGYFLAKDPEDFWRKAEKFLG
IKRKEDLKNIQIQEYVLGVPVYPHYFYSKVREELELMSIDRRYESNVDAIGRIPAKDQLEFDMDITYTVIGNIPIVLRES
LLMDVIEAGERVVKAAEELMGGLWGPFCLEGVFTPDLEFVVFEISARIVAGTNIFVNGSPYTWLRYDRPVSTGRRIAMEI
REAIENDMLEKVLT

Sequences:

>Translated_334_residues
MKVRIATYASHSALQILKGAKDEGFETIAFGSSKVKPLYTKYFPVADYFIEEKYPEEELLNLNAVVVPTGSFVAHLGIEL
VENMKVPYFGNKRVLRWESDRNLERKWLKKAGIRVPEVYEDPDDIEKPVIVKPHGAKGGKGYFLAKDPEDFWRKAEKFLG
IKRKEDLKNIQIQEYVLGVPVYPHYFYSKVREELELMSIDRRYESNVDAIGRIPAKDQLEFDMDITYTVIGNIPIVLRES
LLMDVIEAGERVVKAAEELMGGLWGPFCLEGVFTPDLEFVVFEISARIVAGTNIFVNGSPYTWLRYDRPVSTGRRIAMEI
REAIENDMLEKVLT
>Mature_334_residues
MKVRIATYASHSALQILKGAKDEGFETIAFGSSKVKPLYTKYFPVADYFIEEKYPEEELLNLNAVVVPTGSFVAHLGIEL
VENMKVPYFGNKRVLRWESDRNLERKWLKKAGIRVPEVYEDPDDIEKPVIVKPHGAKGGKGYFLAKDPEDFWRKAEKFLG
IKRKEDLKNIQIQEYVLGVPVYPHYFYSKVREELELMSIDRRYESNVDAIGRIPAKDQLEFDMDITYTVIGNIPIVLRES
LLMDVIEAGERVVKAAEELMGGLWGPFCLEGVFTPDLEFVVFEISARIVAGTNIFVNGSPYTWLRYDRPVSTGRRIAMEI
REAIENDMLEKVLT

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_PYRFU (Q8U0R7)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579246.1
- PDB:   2R84
- PDB:   2R85
- PDB:   2R86
- PDB:   2R87
- PDBsum:   2R84
- PDBsum:   2R85
- PDBsum:   2R86
- PDBsum:   2R87
- ProteinModelPortal:   Q8U0R7
- SMR:   Q8U0R7
- EnsemblBacteria:   EBPYRT00000004030
- GeneID:   1469393
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1517
- NMPDR:   fig|186497.1.peg.1564
- GeneTree:   EBGT00050000022339
- HOGENOM:   HBG392202
- OMA:   VFEISAR
- ProtClustDB:   PRK13278
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 38299; Mature: 38299

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 10-10 BINDING 11-11 BINDING 71-71 BINDING 75-75 BINDING 204-204 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRIATYASHSALQILKGAKDEGFETIAFGSSKVKPLYTKYFPVADYFIEEKYPEEELL
CEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHCCCCHHHHH
NLNAVVVPTGSFVAHLGIELVENMKVPYFGNKRVLRWESDRNLERKWLKKAGIRVPEVYE
CCCEEEEECCHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCC
DPDDIEKPVIVKPHGAKGGKGYFLAKDPEDFWRKAEKFLGIKRKEDLKNIQIQEYVLGVP
CCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHCCEEEHEEECCC
VYPHYFYSKVREELELMSIDRRYESNVDAIGRIPAKDQLEFDMDITYTVIGNIPIVLRES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEECCCEEEEEECCCHHHHHHH
LLMDVIEAGERVVKAAEELMGGLWGPFCLEGVFTPDLEFVVFEISARIVAGTNIFVNGSP
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEEEEEEEECCEEEECCCC
YTWLRYDRPVSTGRRIAMEIREAIENDMLEKVLT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVRIATYASHSALQILKGAKDEGFETIAFGSSKVKPLYTKYFPVADYFIEEKYPEEELL
CEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHCCCCHHHHH
NLNAVVVPTGSFVAHLGIELVENMKVPYFGNKRVLRWESDRNLERKWLKKAGIRVPEVYE
CCCEEEEECCHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCHHHHHHHHHCCCCCCCCC
DPDDIEKPVIVKPHGAKGGKGYFLAKDPEDFWRKAEKFLGIKRKEDLKNIQIQEYVLGVP
CCCCCCCCEEECCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHCCEEEHEEECCC
VYPHYFYSKVREELELMSIDRRYESNVDAIGRIPAKDQLEFDMDITYTVIGNIPIVLRES
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCEECCCEEEEEECCCHHHHHHH
LLMDVIEAGERVVKAAEELMGGLWGPFCLEGVFTPDLEFVVFEISARIVAGTNIFVNGSP
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEEEEEEEEECCEEEECCCC
YTWLRYDRPVSTGRRIAMEIREAIENDMLEKVLT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA