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The map label for this gene is thiI [H]

Identifier: 18977859

GI number: 18977859

Start: 1388969

End: 1389892

Strand: Reverse

Name: thiI [H]

Synonym: PF1487

Alternate gene names: 18977859

Gene position: 1389892-1388969 (Counterclockwise)

Preceding gene: 18977862

Following gene: 18977856

Centisome position: 72.84

GC content: 38.31

Gene sequence:

>924_bases
ATGAACTCTATTATAGTTCGATATGGGGAGATGGGAACAAAATCAAGGCAAACTAGAAAATGGTTTGAGAGAATACTTGT
GAACAACATTAGTGAGGCCCTAGTAAGTGAGGGAATTGAATACAAAGAAGTATTCTCGAAGCATGGGAGGGTTATTGTTA
GGCTAAACGAGGTTGAGAAAGCAAAAGAAGTTCTTCCCAGGGTTTTTGGAATAGTATCCATTTCACCAGCTATGGAGGTT
GAAGCTTCTCTGGATAAGATTAACAAAACAGCTCTAAAGTTATTTAGAAAGAAAAAGAGAGAGCTTGGAATTGAGAAGCC
TAAATTTAGAGTCACTGCTAGGCGAATAACAAAGGAGTTTCCACTAAATAGTATTGAACTTCAGGCAAAGGTTGGTGAAT
TTATTTTGGAATCTGAAGATGGAGAGGTAGATTTGACCAATTATGACATTGAGGTGGGAATTGAACTAATGGAGGGGAAG
GCATACATCTACACCGAAAAAATCTATGGATGGGGAGGTTTGCCTATAGGAACCCAGGGTAAGATGGTGGGAATTTTGGA
TAGTATTGAGGCCGGAGTGGCGATATTCTTAATGATGAAGAGGGGAGTCGAAGTTATTCCCGTTTATGTAGAAGGCCAAG
GCGATGAAGATCTTGTGAGAACCCTTTGGAGAAATCTCAAAAAGTACAGCTACGGAGCTAAGGTAAGATTCACTGTTGTA
AAAAATTTCGAAAGTATTAACGATATCATTAGACATTTTGGGGCAAAAGGAGTCATTAGAGGAATTAGAAAAGAAGTAAT
TGACAGAGAGATAGAAGAGATAATCAGGAGAGATGCAGAACTTTTCATTGTTCCAGTGTATTATCCAGTAATTGCAATCC
CTGACGAAATATTTGAGAAAGTTAGAAATGCAATTTACCCTTGA

Upstream 100 bases:

>100_bases
CCTTCCAACATTTTTTCTTCACCATTTGTTATTAAATTCTTTTACCTTTTTAAATCATTTGGTGAGAAAAGTATTTATTC
CACTTCCCATATGGTCTTCC

Downstream 100 bases:

>100_bases
TACTCTTCTTCAAAGGTTATTCCTCCTCCCTCCCTTCCGTTGTGGAGCTCTTTAATTTCAAATAACTTTAAGGGAACTTT
CTTTAAGGCCTTACCAACAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MNSIIVRYGEMGTKSRQTRKWFERILVNNISEALVSEGIEYKEVFSKHGRVIVRLNEVEKAKEVLPRVFGIVSISPAMEV
EASLDKINKTALKLFRKKKRELGIEKPKFRVTARRITKEFPLNSIELQAKVGEFILESEDGEVDLTNYDIEVGIELMEGK
AYIYTEKIYGWGGLPIGTQGKMVGILDSIEAGVAIFLMMKRGVEVIPVYVEGQGDEDLVRTLWRNLKKYSYGAKVRFTVV
KNFESINDIIRHFGAKGVIRGIRKEVIDREIEEIIRRDAELFIVPVYYPVIAIPDEIFEKVRNAIYP

Sequences:

>Translated_307_residues
MNSIIVRYGEMGTKSRQTRKWFERILVNNISEALVSEGIEYKEVFSKHGRVIVRLNEVEKAKEVLPRVFGIVSISPAMEV
EASLDKINKTALKLFRKKKRELGIEKPKFRVTARRITKEFPLNSIELQAKVGEFILESEDGEVDLTNYDIEVGIELMEGK
AYIYTEKIYGWGGLPIGTQGKMVGILDSIEAGVAIFLMMKRGVEVIPVYVEGQGDEDLVRTLWRNLKKYSYGAKVRFTVV
KNFESINDIIRHFGAKGVIRGIRKEVIDREIEEIIRRDAELFIVPVYYPVIAIPDEIFEKVRNAIYP
>Mature_307_residues
MNSIIVRYGEMGTKSRQTRKWFERILVNNISEALVSEGIEYKEVFSKHGRVIVRLNEVEKAKEVLPRVFGIVSISPAMEV
EASLDKINKTALKLFRKKKRELGIEKPKFRVTARRITKEFPLNSIELQAKVGEFILESEDGEVDLTNYDIEVGIELMEGK
AYIYTEKIYGWGGLPIGTQGKMVGILDSIEAGVAIFLMMKRGVEVIPVYVEGQGDEDLVRTLWRNLKKYSYGAKVRFTVV
KNFESINDIIRHFGAKGVIRGIRKEVIDREIEEIIRRDAELFIVPVYYPVIAIPDEIFEKVRNAIYP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=261, Percent_Identity=28.3524904214559, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 35207; Mature: 35207

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSIIVRYGEMGTKSRQTRKWFERILVNNISEALVSEGIEYKEVFSKHGRVIVRLNEVEK
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHH
AKEVLPRVFGIVSISPAMEVEASLDKINKTALKLFRKKKRELGIEKPKFRVTARRITKEF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
PLNSIELQAKVGEFILESEDGEVDLTNYDIEVGIELMEGKAYIYTEKIYGWGGLPIGTQG
CCCCEEHHHHHHHHHEECCCCCEEEEECCCEECEEEECCCEEEEEEEEECCCCCCCCCCC
KMVGILDSIEAGVAIFLMMKRGVEVIPVYVEGQGDEDLVRTLWRNLKKYSYGAKVRFTVV
CEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEH
KNFESINDIIRHFGAKGVIRGIRKEVIDREIEEIIRRDAELFIVPVYYPVIAIPDEIFEK
HCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHEECCHHHHHH
VRNAIYP
HHHHCCC
>Mature Secondary Structure
MNSIIVRYGEMGTKSRQTRKWFERILVNNISEALVSEGIEYKEVFSKHGRVIVRLNEVEK
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEHHHHH
AKEVLPRVFGIVSISPAMEVEASLDKINKTALKLFRKKKRELGIEKPKFRVTARRITKEF
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHC
PLNSIELQAKVGEFILESEDGEVDLTNYDIEVGIELMEGKAYIYTEKIYGWGGLPIGTQG
CCCCEEHHHHHHHHHEECCCCCEEEEECCCEECEEEECCCEEEEEEEEECCCCCCCCCCC
KMVGILDSIEAGVAIFLMMKRGVEVIPVYVEGQGDEDLVRTLWRNLKKYSYGAKVRFTVV
CEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEH
KNFESINDIIRHFGAKGVIRGIRKEVIDREIEEIIRRDAELFIVPVYYPVIAIPDEIFEK
HCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHEECCHHHHHH
VRNAIYP
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA