Click here to switch to the map view.

The map label for this gene is gatE

Identifier: 18977834

GI number: 18977834

Start: 1369129

End: 1371015

Strand: Direct

Name: gatE

Synonym: PF1462

Alternate gene names: 18977834

Gene position: 1369129-1371015 (Clockwise)

Preceding gene: 18977833

Following gene: 18977835

Centisome position: 71.75

GC content: 41.92

Gene sequence:

>1887_bases
GTGGAGAAAATAAATTATGAAGAGGTAGGACTTAAAGTAGGATTGGAGATTCATAGACAACTAGATACCAAAAAGCTCTT
CTCTCCAGTTCCAAGTAAGCTTAGTGATGACGTTGACTTTACTTTTAAGAGAAGACTAAGACCTACAATGAGTGAACTTG
GGGAGATTGATCCAGCTGCTCTTGAAGAATTCAAGAAGGGAAGAACTTACATTTATGAGGCAAACAACGAACTTGGCGAT
CTAGTTTATATGGATGAAGAACCTCCCAGGGGGCCAGATGAAGAAGCCTTAGAGGTTGCTCTCCAGATAGCATATTTGCT
AAACGCCAAACCAGTTGACGAAGTCTATTATATGAGAAAGATTGTAATAGATGGCTCAAACGTTTCAGGATTCCAGAGAA
CGGCAATAATAGCCACCGACGGAAAAGTCGAAACTCCCTGGGGAACCGTGGGGATCCCAACAATATGTCTAGAGGAAGAT
GCCGCAAGAATTATCGAAACAAGGGATAGGGAAGTCATCTACAGGATTGACAGACTAGGAATTCCCCTTGTAGAGATAAG
CACCACTCCAGATATTCACCACCCAGAGCAGGCTAAGGTAGTTGCAAAGTTCATTGGAGATGCTCTGAGAGCCACTAGAA
AGGTAAAGAGAGGGCTGGGGACAATAAGACAAGATCTAAACGTCTCAATAAAAGGTGGAGCCAGGATTGAAATAAAGGGA
GTTCAAGAGCTCGACATGATTCCAGTGATCATTGAGAGAGAAGTTCAAAGGCAGCTCAACTTGCTCAAGATTAGAGATGA
GCTCAGGAAGAGAGGAGTTACACCAGAAGACATAAAGGAAGAGTTTTACGATGTTACAGATATATTCAAAGACACAAAAT
CAAAAATAATCGCAAGAATTTTGAAGAAAGGAGGAAAAGTTCTCGCAATAAAACTCCCCAAGTTCAAGGGATTAATAGGA
ATGGAAATCCAACCAGGAAGGAGGTTAGGAACAGAATTTGCAGATAGAGCTAAGAAGTACGTGCCTGGAATATTCCACAG
CGATGAGCTTCCAAACTATGGAATAACCCAGGAGGAGGTAGAAAAGGTTAGAAAACTACTTGAATTGGAAGAGGAAGATG
CGTTTGTCCTTGTTGCCGCCCAAGAGGAAATAGCCAAGAAAGCCCTAAAAGAGGTTATAATAAGGGCAAGAGAAGCAATA
ATCGGAGTTCCAGAAGAGACAAGGAGAGCCCTCCCAGACGGAAACACTCAATACATGAGACCTCTCCCAGGGAAAGCAAG
AATGTACCCAGAAACTGACATTCCACCAATAAGGATAACAGAGGAAATGAAGAGGAGAATAAAAGAAAACTTGCCAGAGT
TGCCACAAGCGAAAGTTGAAAAATACGTTAAGGAGTTTGGAATTGACAAGAGCATGGCCCAAACAATTGTGGACGATGAA
AGGGATGAGCTATTTGAAGAGCTGATTGAAATGGGCGTAAAACCATCACTTGCAGCTTCCATTCTGGCAGTTGTTCTTAA
GGGCCTAAGAAAAGAAGTCCCAATTGAAAACATTACAGAGGAGCATATAAAGGGAGCATTCAGGCTGTATTTGGAAGGAA
AAATTGCCAAGGAAGCTTTTGAGGAAATATTCAAAGAGTTAGCTCAGCATCCAGAAAAAACGGCCGAAGAAGTTGCACAA
GAAAAGGGATTAACCTTGTTGAGCGAGGAGGAAGTGAGAAAGATAGTTGACGAAGTAGTTAACCAATACATAGATGTCAT
AAAAGAGAAAGGAATGGGAGCTATGGGACTAATAATGGGTAGAGTGATGACCAAAGTCAGAGGCAAAGCAGATGGAAAGC
TCGTGAGTCAAATAGTTAAGGAGAAGATTAGGGAGATATCCGGTTGA

Upstream 100 bases:

>100_bases
AGGATACTGGACGAGAGCTACTATGAATATGTAACTTGGGGAGAGTAAATCGCAACTCATTTTAACCCCCTCTATTCTTT
ACATTGTTTGGGGAGGAAAA

Downstream 100 bases:

>100_bases
ACTTTCTTTTTTACTACTTTGTTTAGTTTACAAATTTTAAATTTATAATTTTATGTAAACACTAACCAAAATTTTTTATA
AGTCTATTCTCTGGAGTATT

Product: glutamyl-tRNA(Gln) amidotransferase subunit E

Products: NA

Alternate protein names: Glu-ADT subunit E

Number of amino acids: Translated: 628; Mature: 628

Protein sequence:

>628_residues
MEKINYEEVGLKVGLEIHRQLDTKKLFSPVPSKLSDDVDFTFKRRLRPTMSELGEIDPAALEEFKKGRTYIYEANNELGD
LVYMDEEPPRGPDEEALEVALQIAYLLNAKPVDEVYYMRKIVIDGSNVSGFQRTAIIATDGKVETPWGTVGIPTICLEED
AARIIETRDREVIYRIDRLGIPLVEISTTPDIHHPEQAKVVAKFIGDALRATRKVKRGLGTIRQDLNVSIKGGARIEIKG
VQELDMIPVIIEREVQRQLNLLKIRDELRKRGVTPEDIKEEFYDVTDIFKDTKSKIIARILKKGGKVLAIKLPKFKGLIG
MEIQPGRRLGTEFADRAKKYVPGIFHSDELPNYGITQEEVEKVRKLLELEEEDAFVLVAAQEEIAKKALKEVIIRAREAI
IGVPEETRRALPDGNTQYMRPLPGKARMYPETDIPPIRITEEMKRRIKENLPELPQAKVEKYVKEFGIDKSMAQTIVDDE
RDELFEELIEMGVKPSLAASILAVVLKGLRKEVPIENITEEHIKGAFRLYLEGKIAKEAFEEIFKELAQHPEKTAEEVAQ
EKGLTLLSEEEVRKIVDEVVNQYIDVIKEKGMGAMGLIMGRVMTKVRGKADGKLVSQIVKEKIREISG

Sequences:

>Translated_628_residues
MEKINYEEVGLKVGLEIHRQLDTKKLFSPVPSKLSDDVDFTFKRRLRPTMSELGEIDPAALEEFKKGRTYIYEANNELGD
LVYMDEEPPRGPDEEALEVALQIAYLLNAKPVDEVYYMRKIVIDGSNVSGFQRTAIIATDGKVETPWGTVGIPTICLEED
AARIIETRDREVIYRIDRLGIPLVEISTTPDIHHPEQAKVVAKFIGDALRATRKVKRGLGTIRQDLNVSIKGGARIEIKG
VQELDMIPVIIEREVQRQLNLLKIRDELRKRGVTPEDIKEEFYDVTDIFKDTKSKIIARILKKGGKVLAIKLPKFKGLIG
MEIQPGRRLGTEFADRAKKYVPGIFHSDELPNYGITQEEVEKVRKLLELEEEDAFVLVAAQEEIAKKALKEVIIRAREAI
IGVPEETRRALPDGNTQYMRPLPGKARMYPETDIPPIRITEEMKRRIKENLPELPQAKVEKYVKEFGIDKSMAQTIVDDE
RDELFEELIEMGVKPSLAASILAVVLKGLRKEVPIENITEEHIKGAFRLYLEGKIAKEAFEEIFKELAQHPEKTAEEVAQ
EKGLTLLSEEEVRKIVDEVVNQYIDVIKEKGMGAMGLIMGRVMTKVRGKADGKLVSQIVKEKIREISG
>Mature_628_residues
MEKINYEEVGLKVGLEIHRQLDTKKLFSPVPSKLSDDVDFTFKRRLRPTMSELGEIDPAALEEFKKGRTYIYEANNELGD
LVYMDEEPPRGPDEEALEVALQIAYLLNAKPVDEVYYMRKIVIDGSNVSGFQRTAIIATDGKVETPWGTVGIPTICLEED
AARIIETRDREVIYRIDRLGIPLVEISTTPDIHHPEQAKVVAKFIGDALRATRKVKRGLGTIRQDLNVSIKGGARIEIKG
VQELDMIPVIIEREVQRQLNLLKIRDELRKRGVTPEDIKEEFYDVTDIFKDTKSKIIARILKKGGKVLAIKLPKFKGLIG
MEIQPGRRLGTEFADRAKKYVPGIFHSDELPNYGITQEEVEKVRKLLELEEEDAFVLVAAQEEIAKKALKEVIIRAREAI
IGVPEETRRALPDGNTQYMRPLPGKARMYPETDIPPIRITEEMKRRIKENLPELPQAKVEKYVKEFGIDKSMAQTIVDDE
RDELFEELIEMGVKPSLAASILAVVLKGLRKEVPIENITEEHIKGAFRLYLEGKIAKEAFEEIFKELAQHPEKTAEEVAQ
EKGLTLLSEEEVRKIVDEVVNQYIDVIKEKGMGAMGLIMGRVMTKVRGKADGKLVSQIVKEKIREISG

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG2511

COG function: function code J; Archaeal Glu-tRNAGln amidotransferase subunit E (contains GAD domain)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatE subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATE_PYRFU (Q8U0W9)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579191.1
- ProteinModelPortal:   Q8U0W9
- EnsemblBacteria:   EBPYRT00000005639
- GeneID:   1469338
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1462
- NMPDR:   fig|186497.1.peg.1509
- GeneTree:   EBGT00050000022699
- HOGENOM:   HBG297405
- OMA:   TSGFQRT
- ProtClustDB:   PRK04028
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00588
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004115
- InterPro:   IPR023168
- InterPro:   IPR004414
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00134

Pfam domain/function: PF02938 GAD; PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 71237; Mature: 71237

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKINYEEVGLKVGLEIHRQLDTKKLFSPVPSKLSDDVDFTFKRRLRPTMSELGEIDPAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHCCHHHHHHCCCCHHH
LEEFKKGRTYIYEANNELGDLVYMDEEPPRGPDEEALEVALQIAYLLNAKPVDEVYYMRK
HHHHHCCCEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
IVIDGSNVSGFQRTAIIATDGKVETPWGTVGIPTICLEEDAARIIETRDREVIYRIDRLG
HEECCCCCCCCCEEEEEEECCCEECCCCCCCCCEEEECHHHHHHHHCCCHHHHHHHHHCC
IPLVEISTTPDIHHPEQAKVVAKFIGDALRATRKVKRGLGTIRQDLNVSIKGGARIEIKG
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECC
VQELDMIPVIIEREVQRQLNLLKIRDELRKRGVTPEDIKEEFYDVTDIFKDTKSKIIARI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKGGKVLAIKLPKFKGLIGMEIQPGRRLGTEFADRAKKYVPGIFHSDELPNYGITQEEV
HHCCCCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
EKVRKLLELEEEDAFVLVAAQEEIAKKALKEVIIRAREAIIGVPEETRRALPDGNTQYMR
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
PLPGKARMYPETDIPPIRITEEMKRRIKENLPELPQAKVEKYVKEFGIDKSMAQTIVDDE
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCH
RDELFEELIEMGVKPSLAASILAVVLKGLRKEVPIENITEEHIKGAFRLYLEGKIAKEAF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
EEIFKELAQHPEKTAEEVAQEKGLTLLSEEEVRKIVDEVVNQYIDVIKEKGMGAMGLIMG
HHHHHHHHHCHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RVMTKVRGKADGKLVSQIVKEKIREISG
HHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKINYEEVGLKVGLEIHRQLDTKKLFSPVPSKLSDDVDFTFKRRLRPTMSELGEIDPAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHCCHHHHHHCCCCHHH
LEEFKKGRTYIYEANNELGDLVYMDEEPPRGPDEEALEVALQIAYLLNAKPVDEVYYMRK
HHHHHCCCEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
IVIDGSNVSGFQRTAIIATDGKVETPWGTVGIPTICLEEDAARIIETRDREVIYRIDRLG
HEECCCCCCCCCEEEEEEECCCEECCCCCCCCCEEEECHHHHHHHHCCCHHHHHHHHHCC
IPLVEISTTPDIHHPEQAKVVAKFIGDALRATRKVKRGLGTIRQDLNVSIKGGARIEIKG
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECC
VQELDMIPVIIEREVQRQLNLLKIRDELRKRGVTPEDIKEEFYDVTDIFKDTKSKIIARI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKGGKVLAIKLPKFKGLIGMEIQPGRRLGTEFADRAKKYVPGIFHSDELPNYGITQEEV
HHCCCCEEEEECCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
EKVRKLLELEEEDAFVLVAAQEEIAKKALKEVIIRAREAIIGVPEETRRALPDGNTQYMR
HHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCC
PLPGKARMYPETDIPPIRITEEMKRRIKENLPELPQAKVEKYVKEFGIDKSMAQTIVDDE
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCH
RDELFEELIEMGVKPSLAASILAVVLKGLRKEVPIENITEEHIKGAFRLYLEGKIAKEAF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
EEIFKELAQHPEKTAEEVAQEKGLTLLSEEEVRKIVDEVVNQYIDVIKEKGMGAMGLIMG
HHHHHHHHHCHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RVMTKVRGKADGKLVSQIVKEKIREISG
HHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA