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The map label for this gene is gatD

Identifier: 18977833

GI number: 18977833

Start: 1367474

End: 1368790

Strand: Direct

Name: gatD

Synonym: PF1461

Alternate gene names: 18977833

Gene position: 1367474-1368790 (Clockwise)

Preceding gene: 18977831

Following gene: 18977834

Centisome position: 71.66

GC content: 42.07

Gene sequence:

>1317_bases
ATGAGAGTCGAAGAGTTTCTTAAACAAAAGGGAATTGAAGTGGGCGACTATGTCAGGATTATTAAAGTTGAAGACGGAGA
AAAAGTAGAATATGAAGGAATAGTAATGCCCCCTTACGAGCTCTCAGAGGGAGATACGGTTGTAATAAAGCTTGACAATG
GGTATAACATTGGTATTGCAATTGAAAAGATACAAGAAATAAATGTGATAGAAAAAGCTAAGGCTAAGCCCGAAGTGCAC
TTCAAAGCAGAACTTGAGCCTAGGAAAGAGTTACCCACAATAACTATCCTGGGAACTGGAGGGACTATAGCTAGTAGAAT
AGATTACGAGACTGGAGCTGTTTATCCAGCATTTACAGCTGAGGAACTTGCAAAAGCTGTTCCCGAAATTTTTGAGATAG
CAAACATAAAACCTAAGCTACTCTTCAACATATTTAGTGAGGACATGAAGCCTAAACATTGGATTGAGATTGCTCATGAA
ACGGCAAAGGCTCTGAATTCTGGAAACGAAGGAGTTGTGATAGCCCACGGAACAGATACAATGGGATACACTGCAGCAGC
ACTGAGCTTCATGTTGAGAAACTTAACAAAGCCCGTTGTCTTAGTTGGAGCACAGAGAAGTAGCGATAGACCTAGTAGTG
ATGCTGCTATGAATCTTATTTGTGCCACAAGAATGGCAGTAAGCGATGCTGCTGAGGTTATGGTAGTTATGCACGGAGAG
ACAAGCGATACTTACTGCTTAGCTCATAGAGGAACAAAGGTTAGGAAGATGCATACTTCAAGGAGAGATGCTTTTAGGAG
TATAAACGACATTCCCATAGCCAAGATATGGAGTGATGGAAAAATAGAATTCCTTAGAGACGATTACAGGAAGAGAAGCG
AGGGAGAAGTTTGGGTTGATGATAAGCTGGAAGAAAAAGTTGCCCTAGTGAAGGTTTATCCTGGGATGTCAGCAGAGTTA
ATCGACTTCTTAGTGGATAAGGGGTATAAAGGAATAGTAATTGAGGGGACTGGACTAGGGCATACCCCCTCAGATCTCAT
TCCAAGTATAAAGAGGGCGGTTGATGAGGGTGTGGCAGTTTGCATGACGAGCCAGTGCCTTTATGGGAGGGTGAACTTAA
ACGTCTATGCAACTGGAAGAAAGTTGCTTAAGGCAGGGGTTATACCGTGTGAAGACATGCTTCCAGAAACTGCCTACGTA
AAGCTAATGTGGGTTCTCGGTCATACAAATGACTTAAGGGAAGCTAAGAAAATGATGCTCACAAACTATGCTGGGGAAAT
AACCCCCTATACTAAACCAAACACTTTCCTTATTTGA

Upstream 100 bases:

>100_bases
AACTATTCCAAAAGATGAGCCTCCAAATATTATTCCCATAAAAGAAACTTAATATCCAACCTTTATAAGAGTTAATTGCA
AAAATACGCAGGGGGTATAA

Downstream 100 bases:

>100_bases
AACATTTTCTTCACATTTAACATTTTCTTCAAAAAATTTAGTTTTTATGTGTTAAATGTCAAAAAATTTATATACAACTA
AGGCAATATACCAAAGAAAT

Product: glutamyl-tRNA(Gln) amidotransferase subunit D

Products: L-aspartate; NH3

Alternate protein names: Glu-ADT subunit D

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MRVEEFLKQKGIEVGDYVRIIKVEDGEKVEYEGIVMPPYELSEGDTVVIKLDNGYNIGIAIEKIQEINVIEKAKAKPEVH
FKAELEPRKELPTITILGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEIANIKPKLLFNIFSEDMKPKHWIEIAHE
TAKALNSGNEGVVIAHGTDTMGYTAAALSFMLRNLTKPVVLVGAQRSSDRPSSDAAMNLICATRMAVSDAAEVMVVMHGE
TSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIAKIWSDGKIEFLRDDYRKRSEGEVWVDDKLEEKVALVKVYPGMSAEL
IDFLVDKGYKGIVIEGTGLGHTPSDLIPSIKRAVDEGVAVCMTSQCLYGRVNLNVYATGRKLLKAGVIPCEDMLPETAYV
KLMWVLGHTNDLREAKKMMLTNYAGEITPYTKPNTFLI

Sequences:

>Translated_438_residues
MRVEEFLKQKGIEVGDYVRIIKVEDGEKVEYEGIVMPPYELSEGDTVVIKLDNGYNIGIAIEKIQEINVIEKAKAKPEVH
FKAELEPRKELPTITILGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEIANIKPKLLFNIFSEDMKPKHWIEIAHE
TAKALNSGNEGVVIAHGTDTMGYTAAALSFMLRNLTKPVVLVGAQRSSDRPSSDAAMNLICATRMAVSDAAEVMVVMHGE
TSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIAKIWSDGKIEFLRDDYRKRSEGEVWVDDKLEEKVALVKVYPGMSAEL
IDFLVDKGYKGIVIEGTGLGHTPSDLIPSIKRAVDEGVAVCMTSQCLYGRVNLNVYATGRKLLKAGVIPCEDMLPETAYV
KLMWVLGHTNDLREAKKMMLTNYAGEITPYTKPNTFLI
>Mature_438_residues
MRVEEFLKQKGIEVGDYVRIIKVEDGEKVEYEGIVMPPYELSEGDTVVIKLDNGYNIGIAIEKIQEINVIEKAKAKPEVH
FKAELEPRKELPTITILGTGGTIASRIDYETGAVYPAFTAEELAKAVPEIFEIANIKPKLLFNIFSEDMKPKHWIEIAHE
TAKALNSGNEGVVIAHGTDTMGYTAAALSFMLRNLTKPVVLVGAQRSSDRPSSDAAMNLICATRMAVSDAAEVMVVMHGE
TSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIAKIWSDGKIEFLRDDYRKRSEGEVWVDDKLEEKVALVKVYPGMSAEL
IDFLVDKGYKGIVIEGTGLGHTPSDLIPSIKRAVDEGVAVCMTSQCLYGRVNLNVYATGRKLLKAGVIPCEDMLPETAYV
KLMWVLGHTNDLREAKKMMLTNYAGEITPYTKPNTFLI

Specific function: Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-ph

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family. GatD subfamily

Homologues:

Organism=Homo sapiens, GI122937331, Length=360, Percent_Identity=32.7777777777778, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1788065, Length=287, Percent_Identity=35.191637630662, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789327, Length=339, Percent_Identity=33.9233038348083, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI193207372, Length=373, Percent_Identity=32.171581769437, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17558280, Length=373, Percent_Identity=32.171581769437, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI193207374, Length=373, Percent_Identity=32.171581769437, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558278, Length=373, Percent_Identity=32.171581769437, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323189, Length=270, Percent_Identity=31.4814814814815, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323187, Length=270, Percent_Identity=31.4814814814815, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323186, Length=270, Percent_Identity=31.4814814814815, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323184, Length=270, Percent_Identity=31.4814814814815, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320527, Length=347, Percent_Identity=26.2247838616715, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24645530, Length=279, Percent_Identity=34.4086021505376, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639609, Length=308, Percent_Identity=31.8181818181818, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATD_PYRFU (Q8U0X0)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_579190.1
- ProteinModelPortal:   Q8U0X0
- SMR:   Q8U0X0
- EnsemblBacteria:   EBPYRT00000004063
- GeneID:   1469337
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1461
- NMPDR:   fig|186497.1.peg.1508
- GeneTree:   EBGT00050000022811
- HOGENOM:   HBG497495
- OMA:   PEFYHDE
- ProtClustDB:   PRK04183
- GO:   GO:0006412
- HAMAP:   MF_00586
- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827
- InterPro:   IPR011878
- PANTHER:   PTHR11707
- PRINTS:   PR00139
- SMART:   SM00870
- TIGRFAMs:   TIGR00519
- TIGRFAMs:   TIGR02153

Pfam domain/function: PF00710 Asparaginase; SSF53774 Asp/Glutamnse

EC number: 3.5.1.1

Molecular weight: Translated: 48635; Mature: 48635

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00144 ASN_GLN_ASE_1; PS00917 ASN_GLN_ASE_2

Important sites: ACT_SITE 102-102 ACT_SITE 178-178 ACT_SITE 179-179 ACT_SITE 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVEEFLKQKGIEVGDYVRIIKVEDGEKVEYEGIVMPPYELSEGDTVVIKLDNGYNIGIA
CCHHHHHHHCCCCCCCEEEEEEECCCCEEEECCEEECCCCCCCCCEEEEEECCCCEEEEE
IEKIQEINVIEKAKAKPEVHFKAELEPRKELPTITILGTGGTIASRIDYETGAVYPAFTA
EECHHHCCHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCEEHEEECCCCCCCCCCCH
EELAKAVPEIFEIANIKPKLLFNIFSEDMKPKHWIEIAHETAKALNSGNEGVVIAHGTDT
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
MGYTAAALSFMLRNLTKPVVLVGAQRSSDRPSSDAAMNLICATRMAVSDAAEVMVVMHGE
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECC
TSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIAKIWSDGKIEFLRDDYRKRSEGEVWVD
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHCCCCCCEEEC
DKLEEKVALVKVYPGMSAELIDFLVDKGYKGIVIEGTGLGHTPSDLIPSIKRAVDEGVAV
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEE
CMTSQCLYGRVNLNVYATGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTNDLREAKKMML
EEECCEEEEEEEEEEEECCHHHHHHCCCCHHHHCCCCHHEEEEEEECCCHHHHHHHHHHH
TNYAGEITPYTKPNTFLI
HHCCCCCCCCCCCCCEEC
>Mature Secondary Structure
MRVEEFLKQKGIEVGDYVRIIKVEDGEKVEYEGIVMPPYELSEGDTVVIKLDNGYNIGIA
CCHHHHHHHCCCCCCCEEEEEEECCCCEEEECCEEECCCCCCCCCEEEEEECCCCEEEEE
IEKIQEINVIEKAKAKPEVHFKAELEPRKELPTITILGTGGTIASRIDYETGAVYPAFTA
EECHHHCCHHHHHCCCCCEEEEECCCCCCCCCEEEEEECCCCEEHEEECCCCCCCCCCCH
EELAKAVPEIFEIANIKPKLLFNIFSEDMKPKHWIEIAHETAKALNSGNEGVVIAHGTDT
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCC
MGYTAAALSFMLRNLTKPVVLVGAQRSSDRPSSDAAMNLICATRMAVSDAAEVMVVMHGE
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECC
TSDTYCLAHRGTKVRKMHTSRRDAFRSINDIPIAKIWSDGKIEFLRDDYRKRSEGEVWVD
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEHHHHHHHCCCCCCEEEC
DKLEEKVALVKVYPGMSAELIDFLVDKGYKGIVIEGTGLGHTPSDLIPSIKRAVDEGVAV
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEE
CMTSQCLYGRVNLNVYATGRKLLKAGVIPCEDMLPETAYVKLMWVLGHTNDLREAKKMML
EEECCEEEEEEEEEEEECCHHHHHHCCCCHHHHCCCCHHEEEEEEECCCHHHHHHHHHHH
TNYAGEITPYTKPNTFLI
HHCCCCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine; H2O

Specific reaction: L-asparagine + H2O = L-aspartate + NH3

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: 5-Bromo-4-oxo-L-norvaline; Hg2+; NH3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA