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The map label for this gene is nuoB [H]

Identifier: 18977816

GI number: 18977816

Start: 1352727

End: 1353305

Strand: Reverse

Name: nuoB [H]

Synonym: PF1444

Alternate gene names: 18977816

Gene position: 1353305-1352727 (Counterclockwise)

Preceding gene: 18977817

Following gene: 18977815

Centisome position: 70.92

GC content: 41.28

Gene sequence:

>579_bases
ATGGTTGATTGGAGGTTATTTGAGCCATTATTTAATTGGGCCAGAAAAAAGAGTCTTTGGATTGTTGCATTTTGTACCGG
ATGTGGTGGAATAGAGATGCCTCCACTAATGACCGCTAGATATGACTTAGAGAGATTCGGTATAATGCCAGATCCCAGCC
CAAGACAGTATGACCTCTTCCTAATAACGGGTTATGTAACTCCAAAGACTCTAAAGAGAATAATTATAACCTATGAAATG
GCTCCCGATCCCAAGTACGTTCTAGCTCACGGCTCTTGTCCAATTAACGGTGGAATATACTGGGACGCTTATAATGCAAT
AAAACAACTTGACAAGTACATCCCCGTTGACGTATATATAGCTGGATGTATGCCAAGACCCGAGGCTGTAATGGATGGAA
TAAAGAAGCTAATGGAAATGATTGAGAACGGAGAAGCTGATGGATGGAAGAGGTACAAGGAAAACTACGAGTGGTATAGA
AAGAATCAGGATGAGCTATTGGGAGAGGGATGGAGAGAGAAAGAAGCTAGAAAATGGATTCCATGGTTAATGGATAAGAG
GAAGGAGGTTAAAGAATGA

Upstream 100 bases:

>100_bases
CCAAGATTCACAATTGACCAAGCGGCGAAATTCTATTGGAAGTGGCCAACAATAATAGCTGCAATTGGAGCCATAATGGC
TAGCTTGTGAGGTGAGGGGA

Downstream 100 bases:

>100_bases
CGTGGGAAAAGGGAGAAGAGATTGTCAAGCAGATACTAGAGAAAGCTCCATACGCAGAAGGAAAAGTAAGGAGAGAAAGA
AGGCTAGAATTTAGAGTTCC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MVDWRLFEPLFNWARKKSLWIVAFCTGCGGIEMPPLMTARYDLERFGIMPDPSPRQYDLFLITGYVTPKTLKRIIITYEM
APDPKYVLAHGSCPINGGIYWDAYNAIKQLDKYIPVDVYIAGCMPRPEAVMDGIKKLMEMIENGEADGWKRYKENYEWYR
KNQDELLGEGWREKEARKWIPWLMDKRKEVKE

Sequences:

>Translated_192_residues
MVDWRLFEPLFNWARKKSLWIVAFCTGCGGIEMPPLMTARYDLERFGIMPDPSPRQYDLFLITGYVTPKTLKRIIITYEM
APDPKYVLAHGSCPINGGIYWDAYNAIKQLDKYIPVDVYIAGCMPRPEAVMDGIKKLMEMIENGEADGWKRYKENYEWYR
KNQDELLGEGWREKEARKWIPWLMDKRKEVKE
>Mature_192_residues
MVDWRLFEPLFNWARKKSLWIVAFCTGCGGIEMPPLMTARYDLERFGIMPDPSPRQYDLFLITGYVTPKTLKRIIITYEM
APDPKYVLAHGSCPINGGIYWDAYNAIKQLDKYIPVDVYIAGCMPRPEAVMDGIKKLMEMIENGEADGWKRYKENYEWYR
KNQDELLGEGWREKEARKWIPWLMDKRKEVKE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=155, Percent_Identity=37.4193548387097, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1788624, Length=133, Percent_Identity=42.1052631578947, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1789074, Length=121, Percent_Identity=40.495867768595, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=37.1900826446281, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=155, Percent_Identity=35.4838709677419, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859983, Length=163, Percent_Identity=35.5828220858896, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24642371, Length=163, Percent_Identity=35.5828220858896, Blast_Score=118, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24651058, Length=155, Percent_Identity=36.1290322580645, Blast_Score=116, Evalue=1e-26,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 22702; Mature: 22702

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDWRLFEPLFNWARKKSLWIVAFCTGCGGIEMPPLMTARYDLERFGIMPDPSPRQYDLF
CCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEE
LITGYVTPKTLKRIIITYEMAPDPKYVLAHGSCPINGGIYWDAYNAIKQLDKYIPVDVYI
EEEECCCHHHHHHHHEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHCCCEEEEE
AGCMPRPEAVMDGIKKLMEMIENGEADGWKRYKENYEWYRKNQDELLGEGWREKEARKWI
ECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHH
PWLMDKRKEVKE
HHHHHHHHHHCC
>Mature Secondary Structure
MVDWRLFEPLFNWARKKSLWIVAFCTGCGGIEMPPLMTARYDLERFGIMPDPSPRQYDLF
CCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEE
LITGYVTPKTLKRIIITYEMAPDPKYVLAHGSCPINGGIYWDAYNAIKQLDKYIPVDVYI
EEEECCCHHHHHHHHEEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHCCCEEEEE
AGCMPRPEAVMDGIKKLMEMIENGEADGWKRYKENYEWYRKNQDELLGEGWREKEARKWI
ECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHH
PWLMDKRKEVKE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA