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The map label for this gene is hycG [H]

Identifier: 18977804

GI number: 18977804

Start: 1341753

End: 1342256

Strand: Direct

Name: hycG [H]

Synonym: PF1432

Alternate gene names: 18977804

Gene position: 1341753-1342256 (Clockwise)

Preceding gene: 33359564

Following gene: 18977805

Centisome position: 70.31

GC content: 44.64

Gene sequence:

>504_bases
TTGACTAACAATTCGGAGAGAAAAAGGTTGGAGAAGAGAATTGCCCAACTATGTAAGTTCATTGGAAGGTCCCCCTGGGT
CTTTCACGTAAACTCAGGTTCATGTAATGGATGTGACATTGAGATAATCGCAGCATTAACTCCAAGATATGATGCGGAAA
GATTTGGAGTAAAGCTAGTGGGAAGTCCAAGACATGCCGACATTCTTTTAGTCACCGGTCCAGTTACAAACCAAAGCCTC
GAGAGAGTAAAGCTAGTGTATGAACAAACACCAGATCCCAAGATAGTAATAGCCATTGGAGCCTGCCCAACTGGAGGAAG
CGTCTTCTATGAAAGCCCATTTACAAATGCCCCACTTGACAGGATAATCCCAGTAGACGTCTTTGTCCCAGGATGTCCAC
CTAGGCCAGAGGCAATTCTCCATGGAGTCGTATTAGCTCTAGAAAAGCTTGCCAAGATGATAAAAGGTGAAGTTCCGCCT
GAGGAAGGTGAGGAAAATGAGTAA

Upstream 100 bases:

>100_bases
CTAAAAAATGACCATACTGGATTACTGCCCGATTATGTCAGCTATCTCCTAATGACAACTGCATTTATCCTCGTTATACT
CCTACTAAGGGGGTGATAAT

Downstream 100 bases:

>100_bases
AGCTGAAATGGTTGCAAATAAGATAAAGGAGAGGTTTCCAAACGCTGAAGTTGTAGTAAAAACCAACAAGTGGGGAAGAG
AAAGAGTCTGGGTTAGAATA

Product: mbh10 NADH dehydrogenase subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTNNSERKRLEKRIAQLCKFIGRSPWVFHVNSGSCNGCDIEIIAALTPRYDAERFGVKLVGSPRHADILLVTGPVTNQSL
ERVKLVYEQTPDPKIVIAIGACPTGGSVFYESPFTNAPLDRIIPVDVFVPGCPPRPEAILHGVVLALEKLAKMIKGEVPP
EEGEENE

Sequences:

>Translated_167_residues
MTNNSERKRLEKRIAQLCKFIGRSPWVFHVNSGSCNGCDIEIIAALTPRYDAERFGVKLVGSPRHADILLVTGPVTNQSL
ERVKLVYEQTPDPKIVIAIGACPTGGSVFYESPFTNAPLDRIIPVDVFVPGCPPRPEAILHGVVLALEKLAKMIKGEVPP
EEGEENE
>Mature_166_residues
TNNSERKRLEKRIAQLCKFIGRSPWVFHVNSGSCNGCDIEIIAALTPRYDAERFGVKLVGSPRHADILLVTGPVTNQSLE
RVKLVYEQTPDPKIVIAIGACPTGGSVFYESPFTNAPLDRIIPVDVFVPGCPPRPEAILHGVVLALEKLAKMIKGEVPPE
EGEENE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=115, Percent_Identity=39.1304347826087, Blast_Score=111, Evalue=3e-25,
Organism=Escherichia coli, GI1789074, Length=151, Percent_Identity=48.3443708609272, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1788834, Length=149, Percent_Identity=46.3087248322148, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1788624, Length=133, Percent_Identity=36.8421052631579, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=115, Percent_Identity=40.8695652173913, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18859983, Length=118, Percent_Identity=44.0677966101695, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24642371, Length=118, Percent_Identity=44.0677966101695, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24651058, Length=116, Percent_Identity=40.5172413793103, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 18302; Mature: 18171

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNSERKRLEKRIAQLCKFIGRSPWVFHVNSGSCNGCDIEIIAALTPRYDAERFGVKLV
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCCCCHHHCCEEEE
GSPRHADILLVTGPVTNQSLERVKLVYEQTPDPKIVIAIGACPTGGSVFYESPFTNAPLD
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCCCCCHH
RIIPVDVFVPGCPPRPEAILHGVVLALEKLAKMIKGEVPPEEGEENE
HEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TNNSERKRLEKRIAQLCKFIGRSPWVFHVNSGSCNGCDIEIIAALTPRYDAERFGVKLV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEECCCCCHHHCCEEEE
GSPRHADILLVTGPVTNQSLERVKLVYEQTPDPKIVIAIGACPTGGSVFYESPFTNAPLD
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEECCCCCCCHH
RIIPVDVFVPGCPPRPEAILHGVVLALEKLAKMIKGEVPPEEGEENE
HEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]