Click here to switch to the map view.

The map label for this gene is tfb

Identifier: 18977749

GI number: 18977749

Start: 1291998

End: 1292900

Strand: Reverse

Name: tfb

Synonym: PF1377

Alternate gene names: 18977749

Gene position: 1292900-1291998 (Counterclockwise)

Preceding gene: 33359562

Following gene: 18977746

Centisome position: 67.75

GC content: 41.09

Gene sequence:

>903_bases
GTGAATAAGCAAAAGGTTTGTCCTGCTTGTGAATCTGCGGAACTTATTTATGATCCAGAGAGGGGGGAAATAGTCTGTGC
CAAGTGCGGTTATGTAATAGAAGAGAACATAATTGATATGGGTCCTGAGTGGCGTGCTTTTGATGCTTCTCAAAGGGAAC
GCAGGTCTAGAACTGGTGCACCAGAAAGTATTCTTCTTCATGACAAGGGGCTTTCAACTGAAATTGGAATTGACAGATCG
CTTTCCGGATTAATGAGAGAGAAGATGTACCGTTTGAGGAAGTGGCAGTCCAGATTAAGAGTTAGTGATGCAGCAGAGAG
GAACCTAGCTTTTGCCCTAAGTGAGTTGGATAGAATTACTGCTCAGTTAAAACTTCCAAGACATGTAGAGGAAGAAGCTG
CAAGGCTGTACAGAGAGGCAGTGAGAAAGGGACTTATTAGAGGTAGATCTATTGAGAGCGTTATGGCGGCATGTGTTTAC
GCTGCTTGTAGGTTATTAAAAGTTCCCAGGACTCTGGATGAGATTGCTGATATTGCTAGAGTTGATAAAAAGGAAATTGG
AAGAAGTTACAGATTCATTGCGAGAAATCTCAATTTAACTCCCAAAAAACTATTTGTCAAGCCAACTGATTATGTAAATA
AATTTGCGGATGAGCTCGGATTAAGTGAAAAAGTTAGGAGAAGAGCTATTGAAATTCTTGATGAGGCTTATAAAAGGGGG
TTAACTAGTGGTAAGAGTCCAGCTGGTTTAGTAGCAGCAGCCCTATACATAGCTTCTTTATTGGAGGGAGAGAAGAGAAC
ACAAAGAGAAGTTGCCGAAGTTGCTAGAGTAACTGAAGTGACTGTGAGAAATAGATACAAGGAGCTCGTAGAGAAGTTGA
AGATTAAAGTTCCTATAGCATGA

Upstream 100 bases:

>100_bases
TAAGGTACTACCCGCCATAGTAACACAAAAGGTTTATATACCTTTTGGAACTAGCAGTTATATTGGAGAGAGGGGGTTTG
CAGACTGTGGGGGTGTTGAT

Downstream 100 bases:

>100_bases
TTTTATATTTTTTCTGAAAACCTTCGCCATTCAGGGCAGAATAAGTCACATTTTTTACCTGTAGAATCTTTTTTATCAGA
AAGGAGAAGTATGGCGCCGG

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGAPESILLHDKGLSTEIGIDRS
LSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVY
AACRLLKVPRTLDEIADIARVDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPIA

Sequences:

>Translated_300_residues
MNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGAPESILLHDKGLSTEIGIDRS
LSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVY
AACRLLKVPRTLDEIADIARVDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPIA
>Mature_300_residues
MNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGAPESILLHDKGLSTEIGIDRS
LSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRITAQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVY
AACRLLKVPRTLDEIADIARVDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPIA

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=283, Percent_Identity=32.8621908127208, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI22035556, Length=288, Percent_Identity=26.7361111111111, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17564892, Length=301, Percent_Identity=33.5548172757475, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17531397, Length=291, Percent_Identity=24.7422680412371, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325343, Length=304, Percent_Identity=28.9473684210526, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321685, Length=289, Percent_Identity=23.8754325259516, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17136758, Length=307, Percent_Identity=31.9218241042345, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI281361961, Length=290, Percent_Identity=27.5862068965517, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24647658, Length=290, Percent_Identity=27.5862068965517, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_PYRFU (P61998)

Other databases:

- EMBL:   U48391
- EMBL:   AE009950
- PIR:   T46883
- RefSeq:   NP_579106.1
- PDB:   1PFT
- PDBsum:   1PFT
- ProteinModelPortal:   P61998
- EnsemblBacteria:   EBPYRT00000005862
- GeneID:   1469253
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1377
- NMPDR:   fig|186497.1.peg.1424
- GeneTree:   EBGT00050000022592
- HOGENOM:   HBG646536
- OMA:   YKRGLTS
- ProtClustDB:   PRK00423
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 34106; Mature: 34106

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGA
CCCCCCCCCCCCCCEEECCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
PESILLHDKGLSTEIGIDRSLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVYAACRLLKVPRTLDEIADIAR
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG
HHHHHHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPIA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKQKVCPACESAELIYDPERGEIVCAKCGYVIEENIIDMGPEWRAFDASQRERRSRTGA
CCCCCCCCCCCCCCEEECCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
PESILLHDKGLSTEIGIDRSLSGLMREKMYRLRKWQSRLRVSDAAERNLAFALSELDRIT
CCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLKLPRHVEEEAARLYREAVRKGLIRGRSIESVMAACVYAACRLLKVPRTLDEIADIAR
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKKEIGRSYRFIARNLNLTPKKLFVKPTDYVNKFADELGLSEKVRRRAIEILDEAYKRG
HHHHHHHHHHHHHHHCCCCCHHHEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
LTSGKSPAGLVAAALYIASLLEGEKRTQREVAEVARVTEVTVRNRYKELVEKLKIKVPIA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8564536