| Definition | Pyrococcus furiosus DSM 3638, complete genome. |
|---|---|
| Accession | NC_003413 |
| Length | 1,908,256 |
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The map label for this gene is tuf [H]
Identifier: 18977747
GI number: 18977747
Start: 1290148
End: 1291434
Strand: Direct
Name: tuf [H]
Synonym: PF1375
Alternate gene names: 18977747
Gene position: 1290148-1291434 (Clockwise)
Preceding gene: 18977745
Following gene: 18977748
Centisome position: 67.61
GC content: 47.32
Gene sequence:
>1287_bases ATGCCAAAGGACAAGCCCCACGTTAACATTGTATTTATTGGACACGTAGACCACGGAAAGAGCACAACAATTGGAAGGTT ACTCTACGACACAGGAAACATTCCAGAGCAGATCATCAAGAAGTTCGAGGAAATGGGTGAAAAAGGTAAATCCTTCAAGT TCGCTTGGGTCATGGACAGACTTAAGGAAGAGAGAGAAAGAGGTATTACCATTGACGTTGCACACACAAAGTTCGAGACC CCACACAGGTACATCACAATTATCGATGCCCCTGGTCACAGGGACTTCGTAAAGAACATGATTACCGGTGCCTCACAGGC TGATGCTGCAGTTCTCGTAGTTGCAGCTACAGATGGAGTCATGCCACAGACCAAGGAGCACGCCTTCCTTGCAAGGACAC TCGGTATTAAGCATATCATTGTAGCTATCAACAAGATGGATATGGTTAACTACAACCAGAAGAGATTCGAAGAAGTTAAG GCCCAGGTAGAGAAGCTCTTGAAGATGCTCGGTTACAAGGACTTCCCAGTCATCCCAATCAGCGCTTGGGAAGGTGAGAA CGTAGTTAAGAAGAGCGACAAGATGCCATGGTACAACGGCCCAACCCTAATCGAGGCTCTTGATCAGATTCCAGAGCCAG AGAAGCCAGTTGACAAGCCACTCAGAATACCCATCCAGGATGTCTACTCAATCAAGGGTGTCGGTACAGTTCCAGTCGGT AGAGTCGAGACTGGAAAGCTTAGGGTTGGAGAAGTAGTAATATTTGAACCAGCATCCACCATATTCCACAAGCCCATCCA GGGTGAAGTTAAGAGCATCGAGATGCACCACGAGCCACTTGAAGAAGCTCTTCCAGGTGACAACATCGGATTCAACGTCA GAGGTGTTAGCAAGAACGACATCAAGAGAGGCGACGTTGCTGGACACACAACCAACCCACCGACAGTAGTTAGGACAAAG GACACATTCAAGGCTCAAATTATCGTACTCAACCACCCAACTGCAATTACTGTTGGATATAGCCCAGTACTACACGCTCA CACTGCCCAGGTTCCAGTAAGATTCGAGCAACTCCTAGCAAAGCTTGATCCAAAGACCGGTAACATTGTTGAGGAGAACC CACAATTCATCAAGACTGGTGATGCTGCAATCGTAATCCTAAGGCCAATGAAGCCTGTGGTTCTTGAACCAGTCAAAGAG ATTCCACAGCTCGGTAGATTCGCTATCAGAGACATGGGTATGACAATTGCTGCCGGTATGGTCATCTCAATCCAGAGAGG AGAGTGA
Upstream 100 bases:
>100_bases CAACTAAAGGCGGGTTTCCCGCCTGAGGGGGCTGATGTGCGAAAGCTTTAAAAAGTAAGTTCAAAAGTAGGGTTAGGCAA GAAATTGGAGGTGAATGAAA
Downstream 100 bases:
>100_bases TTTCTCTCCCAAATTCTTTTTCTTTTGAGGTGATCAATTATGCAAAAAGCGAGAATCAAGATAGCGAGCACAAATGTTCG TTCTCTTGATGAAGTTGCAA
Product: elongation factor 1-alpha
Products: GDP; phosphate
Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu [H]
Number of amino acids: Translated: 428; Mature: 427
Protein sequence:
>428_residues MPKDKPHVNIVFIGHVDHGKSTTIGRLLYDTGNIPEQIIKKFEEMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFET PHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGVMPQTKEHAFLARTLGIKHIIVAINKMDMVNYNQKRFEEVK AQVEKLLKMLGYKDFPVIPISAWEGENVVKKSDKMPWYNGPTLIEALDQIPEPEKPVDKPLRIPIQDVYSIKGVGTVPVG RVETGKLRVGEVVIFEPASTIFHKPIQGEVKSIEMHHEPLEEALPGDNIGFNVRGVSKNDIKRGDVAGHTTNPPTVVRTK DTFKAQIIVLNHPTAITVGYSPVLHAHTAQVPVRFEQLLAKLDPKTGNIVEENPQFIKTGDAAIVILRPMKPVVLEPVKE IPQLGRFAIRDMGMTIAAGMVISIQRGE
Sequences:
>Translated_428_residues MPKDKPHVNIVFIGHVDHGKSTTIGRLLYDTGNIPEQIIKKFEEMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFET PHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGVMPQTKEHAFLARTLGIKHIIVAINKMDMVNYNQKRFEEVK AQVEKLLKMLGYKDFPVIPISAWEGENVVKKSDKMPWYNGPTLIEALDQIPEPEKPVDKPLRIPIQDVYSIKGVGTVPVG RVETGKLRVGEVVIFEPASTIFHKPIQGEVKSIEMHHEPLEEALPGDNIGFNVRGVSKNDIKRGDVAGHTTNPPTVVRTK DTFKAQIIVLNHPTAITVGYSPVLHAHTAQVPVRFEQLLAKLDPKTGNIVEENPQFIKTGDAAIVILRPMKPVVLEPVKE IPQLGRFAIRDMGMTIAAGMVISIQRGE >Mature_427_residues PKDKPHVNIVFIGHVDHGKSTTIGRLLYDTGNIPEQIIKKFEEMGEKGKSFKFAWVMDRLKEERERGITIDVAHTKFETP HRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGVMPQTKEHAFLARTLGIKHIIVAINKMDMVNYNQKRFEEVKA QVEKLLKMLGYKDFPVIPISAWEGENVVKKSDKMPWYNGPTLIEALDQIPEPEKPVDKPLRIPIQDVYSIKGVGTVPVGR VETGKLRVGEVVIFEPASTIFHKPIQGEVKSIEMHHEPLEEALPGDNIGFNVRGVSKNDIKRGDVAGHTTNPPTVVRTKD TFKAQIIVLNHPTAITVGYSPVLHAHTAQVPVRFEQLLAKLDPKTGNIVEENPQFIKTGDAAIVILRPMKPVVLEPVKEI PQLGRFAIRDMGMTIAAGMVISIQRGE
Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]
COG id: COG5256
COG function: function code J; Translation elongation factor EF-1alpha (GTPase)
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]
Homologues:
Organism=Homo sapiens, GI4503471, Length=451, Percent_Identity=52.549889135255, Blast_Score=463, Evalue=1e-130, Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=52.212389380531, Blast_Score=460, Evalue=1e-129, Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=39.5402298850575, Blast_Score=325, Evalue=5e-89, Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=39.5402298850575, Blast_Score=325, Evalue=7e-89, Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=39.1705069124424, Blast_Score=303, Evalue=2e-82, Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=39.1705069124424, Blast_Score=303, Evalue=2e-82, Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=39.1705069124424, Blast_Score=303, Evalue=2e-82, Organism=Homo sapiens, GI46094014, Length=434, Percent_Identity=38.4792626728111, Blast_Score=300, Evalue=2e-81, Organism=Homo sapiens, GI34147630, Length=432, Percent_Identity=34.4907407407407, Blast_Score=211, Evalue=9e-55, Organism=Homo sapiens, GI54607086, Length=336, Percent_Identity=25.5952380952381, Blast_Score=111, Evalue=1e-24, Organism=Homo sapiens, GI82546879, Length=411, Percent_Identity=25.0608272506083, Blast_Score=97, Evalue=4e-20, Organism=Homo sapiens, GI157426893, Length=307, Percent_Identity=30.6188925081433, Blast_Score=88, Evalue=2e-17, Organism=Homo sapiens, GI4503507, Length=346, Percent_Identity=25.4335260115607, Blast_Score=79, Evalue=6e-15, Organism=Homo sapiens, GI169162126, Length=145, Percent_Identity=34.4827586206897, Blast_Score=78, Evalue=2e-14, Organism=Homo sapiens, GI239752724, Length=145, Percent_Identity=34.4827586206897, Blast_Score=78, Evalue=2e-14, Organism=Homo sapiens, GI169162824, Length=145, Percent_Identity=34.4827586206897, Blast_Score=78, Evalue=2e-14, Organism=Homo sapiens, GI18390331, Length=147, Percent_Identity=36.734693877551, Blast_Score=71, Evalue=2e-12, Organism=Homo sapiens, GI94966754, Length=143, Percent_Identity=32.1678321678322, Blast_Score=69, Evalue=6e-12, Organism=Homo sapiens, GI53729339, Length=253, Percent_Identity=26.4822134387352, Blast_Score=67, Evalue=4e-11, Organism=Homo sapiens, GI53729337, Length=253, Percent_Identity=26.4822134387352, Blast_Score=67, Evalue=4e-11, Organism=Escherichia coli, GI1790412, Length=430, Percent_Identity=34.4186046511628, Blast_Score=212, Evalue=4e-56, Organism=Escherichia coli, GI1789737, Length=430, Percent_Identity=34.4186046511628, Blast_Score=212, Evalue=4e-56, Organism=Escherichia coli, GI1789108, Length=432, Percent_Identity=33.3333333333333, Blast_Score=207, Evalue=1e-54, Organism=Escherichia coli, GI2367247, Length=395, Percent_Identity=29.873417721519, Blast_Score=134, Evalue=1e-32, Organism=Escherichia coli, GI48994988, Length=248, Percent_Identity=32.6612903225806, Blast_Score=100, Evalue=2e-22, Organism=Escherichia coli, GI1789738, Length=152, Percent_Identity=36.1842105263158, Blast_Score=70, Evalue=3e-13, Organism=Escherichia coli, GI1788922, Length=307, Percent_Identity=23.7785016286645, Blast_Score=68, Evalue=1e-12, Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=51.6556291390728, Blast_Score=453, Evalue=1e-128, Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=51.6556291390728, Blast_Score=453, Evalue=1e-128, Organism=Caenorhabditis elegans, GI32566303, Length=446, Percent_Identity=49.1031390134529, Blast_Score=415, Evalue=1e-116, Organism=Caenorhabditis elegans, GI32566629, Length=437, Percent_Identity=37.2997711670481, Blast_Score=288, Evalue=5e-78, Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=37.7622377622378, Blast_Score=286, Evalue=1e-77, Organism=Caenorhabditis elegans, GI115532065, Length=429, Percent_Identity=37.7622377622378, Blast_Score=285, Evalue=4e-77, Organism=Caenorhabditis elegans, GI25141371, Length=362, Percent_Identity=35.6353591160221, Blast_Score=188, Evalue=6e-48, Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=63.0872483221477, Blast_Score=182, Evalue=3e-46, Organism=Caenorhabditis elegans, GI17556456, Length=448, Percent_Identity=33.2589285714286, Blast_Score=176, Evalue=2e-44, Organism=Caenorhabditis elegans, GI17565438, Length=421, Percent_Identity=23.9904988123515, Blast_Score=91, Evalue=8e-19, Organism=Caenorhabditis elegans, GI17557151, Length=244, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=3e-18, Organism=Caenorhabditis elegans, GI17506081, Length=253, Percent_Identity=25.6916996047431, Blast_Score=82, Evalue=5e-16, Organism=Caenorhabditis elegans, GI17509919, Length=342, Percent_Identity=26.0233918128655, Blast_Score=82, Evalue=7e-16, Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=60.9375, Blast_Score=77, Evalue=2e-14, Organism=Saccharomyces cerevisiae, GI6325337, Length=451, Percent_Identity=51.219512195122, Blast_Score=466, Evalue=1e-132, Organism=Saccharomyces cerevisiae, GI6319594, Length=451, Percent_Identity=51.219512195122, Blast_Score=466, Evalue=1e-132, Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=37.2997711670481, Blast_Score=269, Evalue=5e-73, Organism=Saccharomyces cerevisiae, GI6324761, Length=442, Percent_Identity=35.7466063348416, Blast_Score=227, Evalue=3e-60, Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=32.739420935412, Blast_Score=226, Evalue=7e-60, Organism=Saccharomyces cerevisiae, GI6323320, Length=252, Percent_Identity=30.5555555555556, Blast_Score=87, Evalue=5e-18, Organism=Saccharomyces cerevisiae, GI6324650, Length=242, Percent_Identity=26.0330578512397, Blast_Score=75, Evalue=2e-14, Organism=Saccharomyces cerevisiae, GI6320863, Length=341, Percent_Identity=23.4604105571848, Blast_Score=74, Evalue=3e-14, Organism=Saccharomyces cerevisiae, GI6322359, Length=140, Percent_Identity=35.7142857142857, Blast_Score=67, Evalue=4e-12, Organism=Saccharomyces cerevisiae, GI6323098, Length=219, Percent_Identity=31.5068493150685, Blast_Score=64, Evalue=3e-11, Organism=Saccharomyces cerevisiae, GI6324550, Length=188, Percent_Identity=31.3829787234043, Blast_Score=64, Evalue=4e-11, Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=53.6585365853659, Blast_Score=464, Evalue=1e-131, Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=53.6585365853659, Blast_Score=464, Evalue=1e-131, Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=53.1111111111111, Blast_Score=462, Evalue=1e-130, Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=53.1111111111111, Blast_Score=462, Evalue=1e-130, Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=53.1111111111111, Blast_Score=462, Evalue=1e-130, Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=53.1111111111111, Blast_Score=462, Evalue=1e-130, Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=38.1609195402299, Blast_Score=308, Evalue=5e-84, Organism=Drosophila melanogaster, GI17137380, Length=436, Percent_Identity=37.6146788990826, Blast_Score=290, Evalue=9e-79, Organism=Drosophila melanogaster, GI281363316, Length=432, Percent_Identity=36.1111111111111, Blast_Score=233, Evalue=3e-61, Organism=Drosophila melanogaster, GI17864358, Length=432, Percent_Identity=36.1111111111111, Blast_Score=233, Evalue=3e-61, Organism=Drosophila melanogaster, GI19921738, Length=429, Percent_Identity=31.002331002331, Blast_Score=183, Evalue=2e-46, Organism=Drosophila melanogaster, GI19922690, Length=313, Percent_Identity=27.7955271565495, Blast_Score=100, Evalue=3e-21, Organism=Drosophila melanogaster, GI24647048, Length=348, Percent_Identity=24.7126436781609, Blast_Score=86, Evalue=5e-17, Organism=Drosophila melanogaster, GI24647046, Length=348, Percent_Identity=24.7126436781609, Blast_Score=85, Evalue=7e-17, Organism=Drosophila melanogaster, GI78706572, Length=308, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=1e-15, Organism=Drosophila melanogaster, GI28572034, Length=290, Percent_Identity=30, Blast_Score=69, Evalue=5e-12, Organism=Drosophila melanogaster, GI24582462, Length=147, Percent_Identity=34.6938775510204, Blast_Score=68, Evalue=9e-12,
Paralogues:
None
Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR000795 - InterPro: IPR005225 - InterPro: IPR009001 - InterPro: IPR004539 - InterPro: IPR004161 - InterPro: IPR004160 - InterPro: IPR009000 [H]
Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]
EC number: 3.6.5.3
Molecular weight: Translated: 47636; Mature: 47505
Theoretical pI: Translated: 9.32; Mature: 9.32
Prosite motif: PS00301 EFACTOR_GTP
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.0 %Cys (Translated Protein) 3.3 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 0.0 %Cys (Mature Protein) 3.0 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPKDKPHVNIVFIGHVDHGKSTTIGRLLYDTGNIPEQIIKKFEEMGEKGKSFKFAWVMDR CCCCCCEEEEEEEEECCCCCCCCHHEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHH LKEERERGITIDVAHTKFETPHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGV HHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCC MPQTKEHAFLARTLGIKHIIVAINKMDMVNYNQKRFEEVKAQVEKLLKMLGYKDFPVIPI CCCCHHHHHHHHHHCCEEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE SAWEGENVVKKSDKMPWYNGPTLIEALDQIPEPEKPVDKPLRIPIQDVYSIKGVGTVPVG ECCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC RVETGKLRVGEVVIFEPASTIFHKPIQGEVKSIEMHHEPLEEALPGDNIGFNVRGVSKND CCCCCCEEECEEEEECCCHHHHCCCCCCCHHEEEHHHHHHHHHCCCCCCCEEEECCCCCC IKRGDVAGHTTNPPTVVRTKDTFKAQIIVLNHPTAITVGYSPVLHAHTAQVPVRFEQLLA CCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCEEEECCCCEEECCCCCCCHHHHHHHH KLDPKTGNIVEENPQFIKTGDAAIVILRPMKPVVLEPVKEIPQLGRFAIRDMGMTIAAGM HCCCCCCCEECCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCE VISIQRGE EEEEECCC >Mature Secondary Structure PKDKPHVNIVFIGHVDHGKSTTIGRLLYDTGNIPEQIIKKFEEMGEKGKSFKFAWVMDR CCCCCEEEEEEEEECCCCCCCCHHEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHH LKEERERGITIDVAHTKFETPHRYITIIDAPGHRDFVKNMITGASQADAAVLVVAATDGV HHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCEEEEEEECCCC MPQTKEHAFLARTLGIKHIIVAINKMDMVNYNQKRFEEVKAQVEKLLKMLGYKDFPVIPI CCCCHHHHHHHHHHCCEEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEE SAWEGENVVKKSDKMPWYNGPTLIEALDQIPEPEKPVDKPLRIPIQDVYSIKGVGTVPVG ECCCCCCHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHEECCCCCCCCC RVETGKLRVGEVVIFEPASTIFHKPIQGEVKSIEMHHEPLEEALPGDNIGFNVRGVSKND CCCCCCEEECEEEEECCCHHHHCCCCCCCHHEEEHHHHHHHHHCCCCCCCEEEECCCCCC IKRGDVAGHTTNPPTVVRTKDTFKAQIIVLNHPTAITVGYSPVLHAHTAQVPVRFEQLLA CCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCEEEECCCCEEECCCCCCCHHHHHHHH KLDPKTGNIVEENPQFIKTGDAAIVILRPMKPVVLEPVKEIPQLGRFAIRDMGMTIAAGM HCCCCCCCEECCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCE VISIQRGE EEEEECCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: GTP; H2O
Specific reaction: GTP + H2O = GDP + phosphate
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1723106 [H]