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The map label for this gene is aegA [H]

Identifier: 18977699

GI number: 18977699

Start: 1247601

End: 1249025

Strand: Reverse

Name: aegA [H]

Synonym: PF1327

Alternate gene names: 18977699

Gene position: 1249025-1247601 (Counterclockwise)

Preceding gene: 18977700

Following gene: 18977698

Centisome position: 65.45

GC content: 44.98

Gene sequence:

>1425_bases
ATGCCTAGGCTTATTAAAGATAGGGTTCCTACTCCTGAGAGGTCTGTTGGGGAGAGGGTTAGGGATTTTGGTGAGGTTAA
TCTTGGTTATTCTTGGGAGTTGGCTTTGAGGGAGGCTGAGCGTTGTCTCCAGTGTCCTGTTGAGTATGCTCCTTGCATTA
AGGGTTGTCCTGTGCATATTAACATTCCGGGTTTCATTAAAGCCTTGAGGGAGAATAGGGATAATCCTAGTAAGGCTGTG
AGGGAGGCTCTTAGGATTATTTGGAGGGATAATACGTTACCAGCAATTACTGGTAGAGTTTGTCCCCAGGAAGAGCAGTG
TGAGGGTGCTTGTGTGGTTGGAAAGGTTGGTGATCCAATAAACATTGGAAAATTGGAGAGGTTTGTGGCTGATTATGCTA
GGGAGCATGGGATTGACGATGAATTATTACTAGAGGAGATTAAGGGGATTAAGAGGAATGGGAAGAAAGTCGCAATCATT
GGAGCTGGGCCCGCTGGCCTAACTTGTGCCGCAGACCTGGCAAAAATGGGTTATGAGGTAACAATCTACGAAGCCCTCCA
CCAACCAGGAGGAGTACTAATATATGGCATTCCCGAATTTAGGTTACCAAAGGAGATCGTCAAGAAGGAGCTTGAGAATC
TGAGGAGGCTTGGAGTTAAAATTGAAACAAATGTTTTGGTAGGAAAAACTATTACATTTGAAGAGCTTAGGGAAGAGTAC
GATGCAATATTCATAGGAACTGGAGCTGGAACGCCAAGGATATACCCCTGGCCTGGGGTAAATCTCAATGGTATTTATTC
TGCAAATGAGTTCCTCACGAGGATTAACCTAATGAAGGCCTACAAATTCCCAGAATACGACACTCCAATAAAAGTCGGGA
AGAGAGTTGCGGTTATAGGTGGAGGAAACACAGCAATGGATGCTGCTAGGTCAGCACTAAGACTAGGGGCTGAAGTTTGG
ATTCTTTACAGAAGAACAAGGAAGGAGATGACGGCTAGAGAGGAAGAAATAAAGCATGCCGAGGAGGAGGGAGTTAAGTT
CATGTTCTTGGTAACCCCCAAGAGGTTCATTGGGGATGAAAATGGAAACTTAAAAGCTATAGAGCTGGAGAAGATGAAGC
TAGGAGAGCCCGATGAAAGTGGAAGAAGAAGACCAATTCCAACAGGAGAAACCTTCATAATGGAGTTCGATACAGCAATA
ATAGCAATAGGCCAAACCCCGAACAAAACATTCTTGGAGACCGTCCCAGGGCTTAAGGTGGATGAGTGGGGTAGGATTGT
TGTGGATGAGAATTTGATGACTAGTATTCCTGGGGTTTTTGCTGGTGGTGATGCTATTAGGGGTGAGGCTACGGTTATTC
TAGCAATGGGCGACGGGAGAAAAGCAGCAAAAGCCATCCACCAATACCTAAGCAAAGAAAAGTAA

Upstream 100 bases:

>100_bases
CCTTGTCGACTGGGACCAATTGATGAATAGACTTGCTTATTATAGAGATCTGGAAAAGATAAGCCTTGAAAAATGGGAAA
GGGAAAGGAGGATGGTCTAA

Downstream 100 bases:

>100_bases
CGTTTTTATTCCCGCTTTCATTCTCTTTTTTAGGTGGACATATTGGGCAGAGCTGTTAGAACTAAACCTATTGAAATCGT
TGGAATCAAGATAGGGGACA

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MPRLIKDRVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALRENRDNPSKAV
REALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAII
GAGPAGLTCAADLAKMGYEVTIYEALHQPGGVLIYGIPEFRLPKEIVKKELENLRRLGVKIETNVLVGKTITFEELREEY
DAIFIGTGAGTPRIYPWPGVNLNGIYSANEFLTRINLMKAYKFPEYDTPIKVGKRVAVIGGGNTAMDAARSALRLGAEVW
ILYRRTRKEMTAREEEIKHAEEEGVKFMFLVTPKRFIGDENGNLKAIELEKMKLGEPDESGRRRPIPTGETFIMEFDTAI
IAIGQTPNKTFLETVPGLKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKEK

Sequences:

>Translated_474_residues
MPRLIKDRVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALRENRDNPSKAV
REALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAII
GAGPAGLTCAADLAKMGYEVTIYEALHQPGGVLIYGIPEFRLPKEIVKKELENLRRLGVKIETNVLVGKTITFEELREEY
DAIFIGTGAGTPRIYPWPGVNLNGIYSANEFLTRINLMKAYKFPEYDTPIKVGKRVAVIGGGNTAMDAARSALRLGAEVW
ILYRRTRKEMTAREEEIKHAEEEGVKFMFLVTPKRFIGDENGNLKAIELEKMKLGEPDESGRRRPIPTGETFIMEFDTAI
IAIGQTPNKTFLETVPGLKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKEK
>Mature_473_residues
PRLIKDRVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHINIPGFIKALRENRDNPSKAVR
EALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPINIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAIIG
AGPAGLTCAADLAKMGYEVTIYEALHQPGGVLIYGIPEFRLPKEIVKKELENLRRLGVKIETNVLVGKTITFEELREEYD
AIFIGTGAGTPRIYPWPGVNLNGIYSANEFLTRINLMKAYKFPEYDTPIKVGKRVAVIGGGNTAMDAARSALRLGAEVWI
LYRRTRKEMTAREEEIKHAEEEGVKFMFLVTPKRFIGDENGNLKAIELEKMKLGEPDESGRRRPIPTGETFIMEFDTAII
AIGQTPNKTFLETVPGLKVDEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKEK

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=456, Percent_Identity=32.6754385964912, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI111118981, Length=160, Percent_Identity=30.625, Blast_Score=66, Evalue=6e-11,
Organism=Homo sapiens, GI111118983, Length=160, Percent_Identity=30.625, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788811, Length=464, Percent_Identity=38.3620689655172, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=36.864406779661, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI87082180, Length=458, Percent_Identity=37.3362445414847, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1788468, Length=451, Percent_Identity=33.0376940133038, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1789243, Length=428, Percent_Identity=36.6822429906542, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI71984108, Length=471, Percent_Identity=33.7579617834395, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17570289, Length=487, Percent_Identity=31.6221765913758, Blast_Score=191, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17543860, Length=169, Percent_Identity=31.9526627218935, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=29.5918367346939, Blast_Score=189, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28574881, Length=486, Percent_Identity=32.3045267489712, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24665539, Length=486, Percent_Identity=32.3045267489712, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=31.7525773195876, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=31.7525773195876, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI24640763, Length=482, Percent_Identity=30.4979253112033, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI18858217, Length=482, Percent_Identity=30.4979253112033, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 52730; Mature: 52599

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLIKDRVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHI
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEE
NIPGFIKALRENRDNPSKAVREALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPI
ECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCCEEEEECCCCCC
NIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAIIGAGPAGLTCAADLAKMGYEV
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCEE
TIYEALHQPGGVLIYGIPEFRLPKEIVKKELENLRRLGVKIETNVLVGKTITFEELREEY
EEEHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCEEEECEECHHHHHHHH
DAIFIGTGAGTPRIYPWPGVNLNGIYSANEFLTRINLMKAYKFPEYDTPIKVGKRVAVIG
CEEEEECCCCCCEEECCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEE
GGNTAMDAARSALRLGAEVWILYRRTRKEMTAREEEIKHAEEEGVKFMFLVTPKRFIGDE
CCCHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCC
NGNLKAIELEKMKLGEPDESGRRRPIPTGETFIMEFDTAIIAIGQTPNKTFLETVPGLKV
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCCHHHHHCCCCEE
DEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKEK
CCCCCEEECCCHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PRLIKDRVPTPERSVGERVRDFGEVNLGYSWELALREAERCLQCPVEYAPCIKGCPVHI
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEE
NIPGFIKALRENRDNPSKAVREALRIIWRDNTLPAITGRVCPQEEQCEGACVVGKVGDPI
ECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCHHCCCCEEEEECCCCCC
NIGKLERFVADYAREHGIDDELLLEEIKGIKRNGKKVAIIGAGPAGLTCAADLAKMGYEV
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCEE
TIYEALHQPGGVLIYGIPEFRLPKEIVKKELENLRRLGVKIETNVLVGKTITFEELREEY
EEEHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCEEEECEECHHHHHHHH
DAIFIGTGAGTPRIYPWPGVNLNGIYSANEFLTRINLMKAYKFPEYDTPIKVGKRVAVIG
CEEEEECCCCCCEEECCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEE
GGNTAMDAARSALRLGAEVWILYRRTRKEMTAREEEIKHAEEEGVKFMFLVTPKRFIGDE
CCCHHHHHHHHHHHCCHHEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCC
NGNLKAIELEKMKLGEPDESGRRRPIPTGETFIMEFDTAIIAIGQTPNKTFLETVPGLKV
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECEEEEEECCCCCCHHHHHCCCCEE
DEWGRIVVDENLMTSIPGVFAGGDAIRGEATVILAMGDGRKAAKAIHQYLSKEK
CCCCCEEECCCHHHHCCCEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]