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The map label for this gene is thiF [H]

Identifier: 18977661

GI number: 18977661

Start: 1216166

End: 1217128

Strand: Reverse

Name: thiF [H]

Synonym: PF1289

Alternate gene names: 18977661

Gene position: 1217128-1216166 (Counterclockwise)

Preceding gene: 18977664

Following gene: 18977660

Centisome position: 63.78

GC content: 38.73

Gene sequence:

>963_bases
ATGAGAATTGACTTTTCAAGGCATTTTCCCATAATAGGAATTGAAGGACAGAGAAAACTCCAAGAGAAAAAGGTTGCTGT
TGTAGGAGCTGGAGCTTTAGGAAGTTGGGAAGTTTACTTTCTCAAAAAATTGGGGGTTGGTGAAATAATAGTAGTGGATA
GGGATTTTGTAGAGGCGAGTGATATTCCCAGAACAATCTATACGGAAAAAGACATTGGAAGGCCAAAGGTTGACGTGCTT
AGAGATAGGTTTGGTGTTAAAGGATATTTCGAAGATTTAAACCCAGGAACTCTTTATCTCTTAGACGAAGCTGATCTCAT
ACTTGATGGGACGGACAATATATATACTAGACAGGTAATAAATGACTACGCAATAAAAACTGGAAAACCATGGATATATG
TGGGTGTTTTAGCTACTTACGGCAATATAATGCCAATTATCCCAGAAAAAACTGCATGTTTTAGGTGTATAATGCCCAAA
TTGCCCTCCCGTCCTCTACCAACATGTGCCACAGCAGGAATCATGAGCTATGTACCCCCACTAGCTGCTGCAATTGCAGT
AAGCCTCGCAACGAAAATCCTGCTAGGAGAGGAAGTCAAGAGCGAACTCATATTCTTTGACACAAAAACACTAGAATTTG
AAAAAATTGAGATTCCCAGGAGAGATGATTGTCCTGCATGTGTTAGAAAAGAATTAACATTTCTGGAGAAGCAAATAAAA
ATCGAGAGAATGTGTGATGGATCAATACAAGTAGTACCTCCTGAGAGGCTTGAGGTAGACCTTGAAGATCTTGCTAAAAG
ATTAGAAGAAATGGGAATTGAATACATTTTGACCTCTCAGTTTATCCAGTTTGAAGATGAAGACTACGAAGTTCTTATAT
TCAAAGGAGGGAGAATGATTATAAGAGGAGCAGAGGATGAAAAAACTGCAAAGAACATTTTCGCCAGATACTTAGGTGGA
TAA

Upstream 100 bases:

>100_bases
AAAATCTTCATGTATCTTACTTAGTTTTGAAGTCTATAAGGGTTGCTGATCATCAGTTAATTTATTAATTCCCAAATATC
AATCTAAGTAGGGTGAGAGC

Downstream 100 bases:

>100_bases
CAATGATTATTGTTAGATATGGTGAAATTGCAATAAAAAGGGGAAAACGAAAAGAATTTGAAAAAAAGCTAGCTGATAAT
ATAGAAAAAATACTCAAGAG

Product: molybdopterin biosynthesis protein moeb

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRIDFSRHFPIIGIEGQRKLQEKKVAVVGAGALGSWEVYFLKKLGVGEIIVVDRDFVEASDIPRTIYTEKDIGRPKVDVL
RDRFGVKGYFEDLNPGTLYLLDEADLILDGTDNIYTRQVINDYAIKTGKPWIYVGVLATYGNIMPIIPEKTACFRCIMPK
LPSRPLPTCATAGIMSYVPPLAAAIAVSLATKILLGEEVKSELIFFDTKTLEFEKIEIPRRDDCPACVRKELTFLEKQIK
IERMCDGSIQVVPPERLEVDLEDLAKRLEEMGIEYILTSQFIQFEDEDYEVLIFKGGRMIIRGAEDEKTAKNIFARYLGG

Sequences:

>Translated_320_residues
MRIDFSRHFPIIGIEGQRKLQEKKVAVVGAGALGSWEVYFLKKLGVGEIIVVDRDFVEASDIPRTIYTEKDIGRPKVDVL
RDRFGVKGYFEDLNPGTLYLLDEADLILDGTDNIYTRQVINDYAIKTGKPWIYVGVLATYGNIMPIIPEKTACFRCIMPK
LPSRPLPTCATAGIMSYVPPLAAAIAVSLATKILLGEEVKSELIFFDTKTLEFEKIEIPRRDDCPACVRKELTFLEKQIK
IERMCDGSIQVVPPERLEVDLEDLAKRLEEMGIEYILTSQFIQFEDEDYEVLIFKGGRMIIRGAEDEKTAKNIFARYLGG
>Mature_320_residues
MRIDFSRHFPIIGIEGQRKLQEKKVAVVGAGALGSWEVYFLKKLGVGEIIVVDRDFVEASDIPRTIYTEKDIGRPKVDVL
RDRFGVKGYFEDLNPGTLYLLDEADLILDGTDNIYTRQVINDYAIKTGKPWIYVGVLATYGNIMPIIPEKTACFRCIMPK
LPSRPLPTCATAGIMSYVPPLAAAIAVSLATKILLGEEVKSELIFFDTKTLEFEKIEIPRRDDCPACVRKELTFLEKQIK
IERMCDGSIQVVPPERLEVDLEDLAKRLEEMGIEYILTSQFIQFEDEDYEVLIFKGGRMIIRGAEDEKTAKNIFARYLGG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=305, Percent_Identity=28.5245901639344, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI87082356, Length=242, Percent_Identity=31.404958677686, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787048, Length=227, Percent_Identity=26.431718061674, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17540406, Length=244, Percent_Identity=25.4098360655738, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321903, Length=272, Percent_Identity=27.2058823529412, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=30, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322639, Length=186, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325323, Length=171, Percent_Identity=31.5789473684211, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=256, Percent_Identity=27.34375, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 36200; Mature: 36200

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDFSRHFPIIGIEGQRKLQEKKVAVVGAGALGSWEVYFLKKLGVGEIIVVDRDFVEAS
CCCCCCCCCCEEEECCHHHHHHCEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCC
DIPRTIYTEKDIGRPKVDVLRDRFGVKGYFEDLNPGTLYLLDEADLILDGTDNIYTRQVI
CCCCEEECCCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEEECCCEEEECCCCCHHHHHH
NDYAIKTGKPWIYVGVLATYGNIMPIIPEKTACFRCIMPKLPSRPLPTCATAGIMSYVPP
HHHHEECCCCEEEEEEEHHCCCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LAAAIAVSLATKILLGEEVKSELIFFDTKTLEFEKIEIPRRDDCPACVRKELTFLEKQIK
HHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IERMCDGSIQVVPPERLEVDLEDLAKRLEEMGIEYILTSQFIQFEDEDYEVLIFKGGRMI
HHHHCCCCEEEECHHHHEECHHHHHHHHHHCCCEEEEEHHHEEECCCCEEEEEEECCEEE
IRGAEDEKTAKNIFARYLGG
EECCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIDFSRHFPIIGIEGQRKLQEKKVAVVGAGALGSWEVYFLKKLGVGEIIVVDRDFVEAS
CCCCCCCCCCEEEECCHHHHHHCEEEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCC
DIPRTIYTEKDIGRPKVDVLRDRFGVKGYFEDLNPGTLYLLDEADLILDGTDNIYTRQVI
CCCCEEECCCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEEECCCEEEECCCCCHHHHHH
NDYAIKTGKPWIYVGVLATYGNIMPIIPEKTACFRCIMPKLPSRPLPTCATAGIMSYVPP
HHHHEECCCCEEEEEEEHHCCCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
LAAAIAVSLATKILLGEEVKSELIFFDTKTLEFEKIEIPRRDDCPACVRKELTFLEKQIK
HHHHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IERMCDGSIQVVPPERLEVDLEDLAKRLEEMGIEYILTSQFIQFEDEDYEVLIFKGGRMI
HHHHCCCCEEEECHHHHEECHHHHHHHHHHCCCEEEEEHHHEEECCCCEEEEEEECCEEE
IRGAEDEKTAKNIFARYLGG
EECCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]