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The map label for this gene is ascB [C]

Identifier: 18977580

GI number: 18977580

Start: 1148005

End: 1149537

Strand: Reverse

Name: ascB [C]

Synonym: PF1208

Alternate gene names: 18977580

Gene position: 1149537-1148005 (Counterclockwise)

Preceding gene: 18977586

Following gene: 18977579

Centisome position: 60.24

GC content: 44.03

Gene sequence:

>1533_bases
ATGTTCCCTGAAAAGTTCCTTTGGGGTGTGGCACAATCGGGTTTTCAGTTTGAAATGGGGGATAAACTCAGGAGGAATAT
TGACACTAACACTGATTGGTGGCACTGGGTAAGGGATAAGACAAATATAGAGAAAGGCCTCGTTAGTGGAGATCTTCCCG
AGGAGGGGATTAACAATTACGAGCTTTATGAGAAGGACCATGAGATTGCAAGAAAGCTGGGTCTTAATGCTTACAGAATA
GGCATAGAGTGGAGCAGAATATTCCCATGGCCAACGACATTTATTGATGTTGATTATAGCTATAATGAATCATATAACCT
TATAGAAGATGTAAAGATCACCAAGGACACTTTGGAGGAGTTAGATGAGATCGCCAACAAGAGGGAGGTGGCCTACTATA
GGTCAGTCATAAACAGCCTGAGGAGCAAGGGGTTTAAGGTTATAGTTAATCTAAATCACTTCACCCTTCCATATTGGTTG
CATGATCCCATTGAGGCTAGGGAGAGGGCGTTAACTAATAAGAGGAACGGCTGGGTTAACCCAAGAACAGTTATAGAGTT
TGCAAAGTATGCCGCTTACATAGCCTATAAGTTTGGAGATATAGTGGATATGTGGAGCACGTTTAATGAGCCTATGGTGG
TTGTTGAGCTTGGCTACCTAGCCCCCTACTCTGGCTTCCCTCCAGGGGTTCTAAATCCAGAGGCCGCAAAGCTGGCGATA
CTTCACATGATAAATGCACATGCTTTAGCTTATAGGCAGATAAAGAAGTTTGACACTGAGAAAGCTGATAAGGATTCTAA
AGAGCCTGCAGAAGTTGGTATAATTTACAACAACATTGGAGTTGCTTATCCCAAGGATCCGAACGATTCCAAGGATGTTA
AGGCAGCAGAAAACGACAACTTCTTCCACTCAGGGCTGTTCTTCGAGGCCATACACAAAGGAAAACTTAATATAGAGTTT
GACGGTGAAACGTTTATAGATGCCCCCTATCTAAAGGGCAATGACTGGATAGGGGTTAATTACTACACAAGGGAAGTAGT
TACGTATCAGGAACCAATGTTTCCTTCAATCCCGCTGATCACCTTTAAGGGAGTTCAAGGATATGGCTATGCCTGCAGAC
CTGGAACTCTGTCAAAGGATGACAGACCCGTCAGCGACATAGGATGGGAACTCTATCCAGAGGGGATGTACGATTCAATA
GTTGAAGCTCACAAGTACGGCGTTCCAGTTTACGTGACGGAGAACGGAATAGCGGATTCAAAGGACATCCTAAGACCTTA
CTACATAGCGAGCCACATAAAGATGATAGAGAAGGCCTTTGAGGATGGGTATGAAGTTAAGGGCTACTTCCACTGGGCAT
TAACTGACAACTTCGAGTGGGCTCTCGGGTTTAGAATGCGCTTTGGCCTCTACGAAGTCAACCTAATTACAAAGGAGAGA
ATTCCCAGGGAGAAGAGCGTGTCGATATTCAGAGAGATAGTAGCCAATAATGGTGTTACGAAAAAGATTGAAGAGGAATT
GCTGAGGGGATGA

Upstream 100 bases:

>100_bases
GCTGTATATTTATAATCATTGGGCCAAATTATAGAAATTTAGAAAGCCTTATAAGATATGATAACGATACTCACTATTGA
CAATATAAGGGGGGAGGATC

Downstream 100 bases:

>100_bases
AATATGAAGACAATAGCAGTTGATGAGGAAACATGGGAGGCAATTAAAAAGCTCAAGGCTAGGTTGGATGCAAAATCATA
CGATGAAGTCTTGAAAAAGC

Product: beta-mannosidase

Products: NA

Alternate protein names: Lactase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MFPEKFLWGVAQSGFQFEMGDKLRRNIDTNTDWWHWVRDKTNIEKGLVSGDLPEEGINNYELYEKDHEIARKLGLNAYRI
GIEWSRIFPWPTTFIDVDYSYNESYNLIEDVKITKDTLEELDEIANKREVAYYRSVINSLRSKGFKVIVNLNHFTLPYWL
HDPIEARERALTNKRNGWVNPRTVIEFAKYAAYIAYKFGDIVDMWSTFNEPMVVVELGYLAPYSGFPPGVLNPEAAKLAI
LHMINAHALAYRQIKKFDTEKADKDSKEPAEVGIIYNNIGVAYPKDPNDSKDVKAAENDNFFHSGLFFEAIHKGKLNIEF
DGETFIDAPYLKGNDWIGVNYYTREVVTYQEPMFPSIPLITFKGVQGYGYACRPGTLSKDDRPVSDIGWELYPEGMYDSI
VEAHKYGVPVYVTENGIADSKDILRPYYIASHIKMIEKAFEDGYEVKGYFHWALTDNFEWALGFRMRFGLYEVNLITKER
IPREKSVSIFREIVANNGVTKKIEEELLRG

Sequences:

>Translated_510_residues
MFPEKFLWGVAQSGFQFEMGDKLRRNIDTNTDWWHWVRDKTNIEKGLVSGDLPEEGINNYELYEKDHEIARKLGLNAYRI
GIEWSRIFPWPTTFIDVDYSYNESYNLIEDVKITKDTLEELDEIANKREVAYYRSVINSLRSKGFKVIVNLNHFTLPYWL
HDPIEARERALTNKRNGWVNPRTVIEFAKYAAYIAYKFGDIVDMWSTFNEPMVVVELGYLAPYSGFPPGVLNPEAAKLAI
LHMINAHALAYRQIKKFDTEKADKDSKEPAEVGIIYNNIGVAYPKDPNDSKDVKAAENDNFFHSGLFFEAIHKGKLNIEF
DGETFIDAPYLKGNDWIGVNYYTREVVTYQEPMFPSIPLITFKGVQGYGYACRPGTLSKDDRPVSDIGWELYPEGMYDSI
VEAHKYGVPVYVTENGIADSKDILRPYYIASHIKMIEKAFEDGYEVKGYFHWALTDNFEWALGFRMRFGLYEVNLITKER
IPREKSVSIFREIVANNGVTKKIEEELLRG
>Mature_510_residues
MFPEKFLWGVAQSGFQFEMGDKLRRNIDTNTDWWHWVRDKTNIEKGLVSGDLPEEGINNYELYEKDHEIARKLGLNAYRI
GIEWSRIFPWPTTFIDVDYSYNESYNLIEDVKITKDTLEELDEIANKREVAYYRSVINSLRSKGFKVIVNLNHFTLPYWL
HDPIEARERALTNKRNGWVNPRTVIEFAKYAAYIAYKFGDIVDMWSTFNEPMVVVELGYLAPYSGFPPGVLNPEAAKLAI
LHMINAHALAYRQIKKFDTEKADKDSKEPAEVGIIYNNIGVAYPKDPNDSKDVKAAENDNFFHSGLFFEAIHKGKLNIEF
DGETFIDAPYLKGNDWIGVNYYTREVVTYQEPMFPSIPLITFKGVQGYGYACRPGTLSKDDRPVSDIGWELYPEGMYDSI
VEAHKYGVPVYVTENGIADSKDILRPYYIASHIKMIEKAFEDGYEVKGYFHWALTDNFEWALGFRMRFGLYEVNLITKER
IPREKSVSIFREIVANNGVTKKIEEELLRG

Specific function: Can Hydrolyze Salicin, Cellobiose, And Probably Arbutin. [C]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=533, Percent_Identity=27.3921200750469, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI110681710, Length=548, Percent_Identity=27.5547445255474, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI13273313, Length=536, Percent_Identity=25, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1789070, Length=541, Percent_Identity=23.8447319778189, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI2367174, Length=536, Percent_Identity=22.3880597014925, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI2367270, Length=534, Percent_Identity=23.7827715355805, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17539390, Length=519, Percent_Identity=26.2042389210019, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17552856, Length=527, Percent_Identity=24.6679316888046, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21356577, Length=543, Percent_Identity=25.9668508287293, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 59069; Mature: 59069

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPEKFLWGVAQSGFQFEMGDKLRRNIDTNTDWWHWVRDKTNIEKGLVSGDLPEEGINNY
CCCHHHHHHHHCCCCEECCCHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCCCHHCCCCC
ELYEKDHEIARKLGLNAYRIGIEWSRIFPWPTTFIDVDYSYNESYNLIEDVKITKDTLEE
HHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHCEECHHHHHH
LDEIANKREVAYYRSVINSLRSKGFKVIVNLNHFTLPYWLHDPIEARERALTNKRNGWVN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCEECCHHHHHHHHHHCCCCCCCC
PRTVIEFAKYAAYIAYKFGDIVDMWSTFNEPMVVVELGYLAPYSGFPPGVLNPEAAKLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHH
LHMINAHALAYRQIKKFDTEKADKDSKEPAEVGIIYNNIGVAYPKDPNDSKDVKAAENDN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCCC
FFHSGLFFEAIHKGKLNIEFDGETFIDAPYLKGNDWIGVNYYTREVVTYQEPMFPSIPLI
CEECCHHHHHHHCCEEEEEECCCEEECCCEECCCCEEEEEEEEEEEEEECCCCCCCCCEE
TFKGVQGYGYACRPGTLSKDDRPVSDIGWELYPEGMYDSIVEAHKYGVPVYVTENGIADS
EEECCCCCCEEECCCCCCCCCCCHHHCCCEECCCCHHHHHHHHHHCCCCEEEECCCCCCC
KDILRPYYIASHIKMIEKAFEDGYEVKGYFHWALTDNFEWALGFRMRFGLYEVNLITKER
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEEEECEEEEEEEEHHH
IPREKSVSIFREIVANNGVTKKIEEELLRG
CCCHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MFPEKFLWGVAQSGFQFEMGDKLRRNIDTNTDWWHWVRDKTNIEKGLVSGDLPEEGINNY
CCCHHHHHHHHCCCCEECCCHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCCCHHCCCCC
ELYEKDHEIARKLGLNAYRIGIEWSRIFPWPTTFIDVDYSYNESYNLIEDVKITKDTLEE
HHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEECCCCCCCCCHHHCEECHHHHHH
LDEIANKREVAYYRSVINSLRSKGFKVIVNLNHFTLPYWLHDPIEARERALTNKRNGWVN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCEECCHHHHHHHHHHCCCCCCCC
PRTVIEFAKYAAYIAYKFGDIVDMWSTFNEPMVVVELGYLAPYSGFPPGVLNPEAAKLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHH
LHMINAHALAYRQIKKFDTEKADKDSKEPAEVGIIYNNIGVAYPKDPNDSKDVKAAENDN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCCC
FFHSGLFFEAIHKGKLNIEFDGETFIDAPYLKGNDWIGVNYYTREVVTYQEPMFPSIPLI
CEECCHHHHHHHCCEEEEEECCCEEECCCEECCCCEEEEEEEEEEEEEECCCCCCCCCEE
TFKGVQGYGYACRPGTLSKDDRPVSDIGWELYPEGMYDSIVEAHKYGVPVYVTENGIADS
EEECCCCCCEEECCCCCCCCCCCHHHCCCEECCCCHHHHHHHHHHCCCCEEEECCCCCCC
KDILRPYYIASHIKMIEKAFEDGYEVKGYFHWALTDNFEWALGFRMRFGLYEVNLITKER
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCEEEEEEEEECEEEEEEEEHHH
IPREKSVSIFREIVANNGVTKKIEEELLRG
CCCHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10833397 [H]