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The map label for this gene is nirB [C]

Identifier: 18977558

GI number: 18977558

Start: 1131551

End: 1132879

Strand: Reverse

Name: nirB [C]

Synonym: PF1186

Alternate gene names: 18977558

Gene position: 1132879-1131551 (Counterclockwise)

Preceding gene: 18977559

Following gene: 18977556

Centisome position: 59.37

GC content: 44.39

Gene sequence:

>1329_bases
ATGGAAAAGAAAAAGGTAGTCATAATTGGTGGTGGAGCAGCAGGAATGAGTGCCGCTTCTAGAGTAAAAAGATTGAGGCC
TGAATGGGACGTTAAAGTTTTCGAGGCAACGGAGTGGGTGAGCCATGCTCCTTGTGGTATCCCGTATGTAGTTGAAGGTA
TCGCACCAAAAGAAAAGCTCATGCACTATCCTCCAGAAGTGTTCATTAAGAAGAGAGGAATTGACCTTCACTTAAAAGCT
GAAGTTATTGAGGTCGAGACGGGATACGTGAGAGTTAGGGAGAATGGAGAAGAAAAGAGCTATGAGTGGGATTATCTGGT
GTTCGCCAATGGAGCTTCTCCCCAGATTCCTGAAATAGAAGGAGTGGACTTACCAGGGGTATTTACAGCTGATTTACCTC
CGGATGCCGTAGCTATAACAGAATATATGGAGAAGAATAAGGTTGAAGATGTTGTGATTATAGGAACTGGTTACATAGCC
TTAGAGATGGCCGAGGCCTTTGTTACTAGAGGGAAGAATGTAACTCTAATCGGGAGAAGCGAGAGAGTTCTTAGAAAGAC
CTTTGACAAGGAAATAACAGATATCGTGGAGGAAAAGCTTAGACAGCATTTAAACTTGAGGTTACATGAGAAAACCCTTA
GCATAGAGGGAAGGGAGAGAGTAGAAAAAGTGATTACAGATGGGGGAGAATACAAGGCCGACTTAGTAATAATAGCTACG
GGGATAAAGCCCAACGTTGAATTAGCAAAGCAATTGGGGGTAAAGATAGGAGAGACAGGTGCAATATGGACTAACGAAAA
AATGCAGACTAGCGTTGAGAATGTATATGCCGCAGGGGATGTTGCCGAAACAAAACATGTAATTACTGGGAAGAGAGTGT
GGATCCCCTTAGCTCCTGCAGGTAATAAAATGGGGTACGTTGCTGGAAGCAACATAGCTGGAAAGGAGATAGAATTCCCA
GGAGTCCTGGGAACTAGTATTACCAAGTTCATGGACTTGGAAATCGGAAAAACGGGGTTAACCGAAAATGAGGCTGTAAA
AGAAGGATATGATGTTAGGACAGCATTCATTAAGGCAAATACTAAGCCTCACTATTACCCAGGAGGAAGGGAGATATGGC
TAAAGGGAGTTGTGGATAATGAAACCAACAGGCTCTTAGGAGTGCAGGCAGTTGGAGCTGAAATTCTTCCCAGGATCGAC
AGTGCTGCAGCAATGATTACAGCTGGCTTCACAACTAAGGATGTCTTCTTCACAGACCTAGCCTATGCACCACCCTTTGC
CCCGGTTTGGGATCCCTTGATAGTCCTCGCCAGGGTTTTGAAGTTCTAG

Upstream 100 bases:

>100_bases
GGAAGCGTTCTAGATTGGGTAAGACTTCCCCTAAATGTTTGAATTTCTTCCCCACTAAAATTTTTTAACTAAAGGTTTTA
AACCTGGGATGGGTGAGATT

Downstream 100 bases:

>100_bases
CTCTTTCCATATATTTCATTTACATACATTAGGAATTCACTTGCCTTTACTACATCCCTCACATCAATTCTTTCGTTCTT
TGTGTGTGAGATGTCAAGGT

Product: NADH oxidase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MEKKKVVIIGGGAAGMSAASRVKRLRPEWDVKVFEATEWVSHAPCGIPYVVEGIAPKEKLMHYPPEVFIKKRGIDLHLKA
EVIEVETGYVRVRENGEEKSYEWDYLVFANGASPQIPEIEGVDLPGVFTADLPPDAVAITEYMEKNKVEDVVIIGTGYIA
LEMAEAFVTRGKNVTLIGRSERVLRKTFDKEITDIVEEKLRQHLNLRLHEKTLSIEGRERVEKVITDGGEYKADLVIIAT
GIKPNVELAKQLGVKIGETGAIWTNEKMQTSVENVYAAGDVAETKHVITGKRVWIPLAPAGNKMGYVAGSNIAGKEIEFP
GVLGTSITKFMDLEIGKTGLTENEAVKEGYDVRTAFIKANTKPHYYPGGREIWLKGVVDNETNRLLGVQAVGAEILPRID
SAAAMITAGFTTKDVFFTDLAYAPPFAPVWDPLIVLARVLKF

Sequences:

>Translated_442_residues
MEKKKVVIIGGGAAGMSAASRVKRLRPEWDVKVFEATEWVSHAPCGIPYVVEGIAPKEKLMHYPPEVFIKKRGIDLHLKA
EVIEVETGYVRVRENGEEKSYEWDYLVFANGASPQIPEIEGVDLPGVFTADLPPDAVAITEYMEKNKVEDVVIIGTGYIA
LEMAEAFVTRGKNVTLIGRSERVLRKTFDKEITDIVEEKLRQHLNLRLHEKTLSIEGRERVEKVITDGGEYKADLVIIAT
GIKPNVELAKQLGVKIGETGAIWTNEKMQTSVENVYAAGDVAETKHVITGKRVWIPLAPAGNKMGYVAGSNIAGKEIEFP
GVLGTSITKFMDLEIGKTGLTENEAVKEGYDVRTAFIKANTKPHYYPGGREIWLKGVVDNETNRLLGVQAVGAEILPRID
SAAAMITAGFTTKDVFFTDLAYAPPFAPVWDPLIVLARVLKF
>Mature_442_residues
MEKKKVVIIGGGAAGMSAASRVKRLRPEWDVKVFEATEWVSHAPCGIPYVVEGIAPKEKLMHYPPEVFIKKRGIDLHLKA
EVIEVETGYVRVRENGEEKSYEWDYLVFANGASPQIPEIEGVDLPGVFTADLPPDAVAITEYMEKNKVEDVVIIGTGYIA
LEMAEAFVTRGKNVTLIGRSERVLRKTFDKEITDIVEEKLRQHLNLRLHEKTLSIEGRERVEKVITDGGEYKADLVIIAT
GIKPNVELAKQLGVKIGETGAIWTNEKMQTSVENVYAAGDVAETKHVITGKRVWIPLAPAGNKMGYVAGSNIAGKEIEFP
GVLGTSITKFMDLEIGKTGLTENEAVKEGYDVRTAFIKANTKPHYYPGGREIWLKGVVDNETNRLLGVQAVGAEILPRID
SAAAMITAGFTTKDVFFTDLAYAPPFAPVWDPLIVLARVLKF

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI21389617, Length=323, Percent_Identity=23.8390092879257, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226437568, Length=323, Percent_Identity=23.8390092879257, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI65787454, Length=323, Percent_Identity=23.8390092879257, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI50301238, Length=352, Percent_Identity=25.2840909090909, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789765, Length=411, Percent_Identity=25.5474452554745, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1786307, Length=347, Percent_Identity=28.2420749279539, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789065, Length=289, Percent_Identity=24.5674740484429, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788892, Length=293, Percent_Identity=25.5972696245734, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=24.235807860262, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789915, Length=349, Percent_Identity=25.5014326647564, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17557007, Length=322, Percent_Identity=25.776397515528, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71983419, Length=310, Percent_Identity=28.7096774193548, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71983429, Length=310, Percent_Identity=28.7096774193548, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=221, Percent_Identity=25.7918552036199, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325166, Length=322, Percent_Identity=25.776397515528, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24585130, Length=321, Percent_Identity=26.791277258567, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639257, Length=218, Percent_Identity=29.8165137614679, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281359715, Length=218, Percent_Identity=29.8165137614679, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281359713, Length=218, Percent_Identity=29.8165137614679, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639250, Length=218, Percent_Identity=29.8165137614679, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18543267, Length=218, Percent_Identity=29.8165137614679, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24639252, Length=218, Percent_Identity=29.8165137614679, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CDR_PYRFU (Q8U1M0)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578915.1
- ProteinModelPortal:   Q8U1M0
- SMR:   Q8U1M0
- EnsemblBacteria:   EBPYRT00000005950
- GeneID:   1469056
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1186
- NMPDR:   fig|186497.1.peg.1227
- GeneTree:   EBGT00050000022426
- HOGENOM:   HBG535576
- OMA:   MQTSVEN
- ProtClustDB:   CLSK541377
- BRENDA:   1.8.1.14
- GO:   GO:0005737
- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PRINTS:   PR00368
- TIGRFAMs:   TIGR03385

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.14

Molecular weight: Translated: 48721; Mature: 48721

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: ACT_SITE 45-45 ACT_SITE 45-45 BINDING 17-17 BINDING 21-21 BINDING 24-24 BINDING 41-41 BINDING 72-72 BINDING 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKKVVIIGGGAAGMSAASRVKRLRPEWDVKVFEATEWVSHAPCGIPYVVEGIAPKEKL
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCEECCCCCHHHH
MHYPPEVFIKKRGIDLHLKAEVIEVETGYVRVRENGEEKSYEWDYLVFANGASPQIPEIE
HCCCCHHEEEECCCEEEEEEEEEEEECCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCC
GVDLPGVFTADLPPDAVAITEYMEKNKVEDVVIIGTGYIALEMAEAFVTRGKNVTLIGRS
CCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECC
ERVLRKTFDKEITDIVEEKLRQHLNLRLHEKTLSIEGRERVEKVITDGGEYKADLVIIAT
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEE
GIKPNVELAKQLGVKIGETGAIWTNEKMQTSVENVYAAGDVAETKHVITGKRVWIPLAPA
CCCCCHHHHHHCCCEECCCCCEEECHHHHHHHHHHEECCCHHHCCEEEECCEEEEEECCC
GNKMGYVAGSNIAGKEIEFPGVLGTSITKFMDLEIGKTGLTENEAVKEGYDVRTAFIKAN
CCCEEEEECCCCCCCEEECCCCHHHHHHHHHEEECCCCCCCCCHHHHCCCCEEEEEEEEC
TKPHYYPGGREIWLKGVVDNETNRLLGVQAVGAEILPRIDSAAAMITAGFTTKDVFFTDL
CCCCCCCCCCEEEEEEEECCCCCCEEEEEHHCHHHHCCCCCCCEEEEECCCCCCEEEEHH
AYAPPFAPVWDPLIVLARVLKF
CCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKKKVVIIGGGAAGMSAASRVKRLRPEWDVKVFEATEWVSHAPCGIPYVVEGIAPKEKL
CCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCCCEECCCCCHHHH
MHYPPEVFIKKRGIDLHLKAEVIEVETGYVRVRENGEEKSYEWDYLVFANGASPQIPEIE
HCCCCHHEEEECCCEEEEEEEEEEEECCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCC
GVDLPGVFTADLPPDAVAITEYMEKNKVEDVVIIGTGYIALEMAEAFVTRGKNVTLIGRS
CCCCCCEEECCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCEEEEEECC
ERVLRKTFDKEITDIVEEKLRQHLNLRLHEKTLSIEGRERVEKVITDGGEYKADLVIIAT
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEEEEEEE
GIKPNVELAKQLGVKIGETGAIWTNEKMQTSVENVYAAGDVAETKHVITGKRVWIPLAPA
CCCCCHHHHHHCCCEECCCCCEEECHHHHHHHHHHEECCCHHHCCEEEECCEEEEEECCC
GNKMGYVAGSNIAGKEIEFPGVLGTSITKFMDLEIGKTGLTENEAVKEGYDVRTAFIKAN
CCCEEEEECCCCCCCEEECCCCHHHHHHHHHEEECCCCCCCCCHHHHCCCCEEEEEEEEC
TKPHYYPGGREIWLKGVVDNETNRLLGVQAVGAEILPRIDSAAAMITAGFTTKDVFFTDL
CCCCCCCCCCEEEEEEEECCCCCCEEEEEHHCHHHHCCCCCCCEEEEECCCCCCEEEEHH
AYAPPFAPVWDPLIVLARVLKF
CCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA