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The map label for this gene is panB

Identifier: 18977515

GI number: 18977515

Start: 1096655

End: 1097506

Strand: Direct

Name: panB

Synonym: PF1143

Alternate gene names: 18977515

Gene position: 1096655-1097506 (Clockwise)

Preceding gene: 18977514

Following gene: 18977518

Centisome position: 57.47

GC content: 44.95

Gene sequence:

>852_bases
ATGAGAGAAATCACCCCCAAGAGAATTATGGAAATGAAGGGAAAAGAAAAGATCACTATGATAACAGCCTATGATTATCC
CTCAGCACTTCTTGCAGATAAGGCAGGTTTTGACATAGTCTTTGTCGGTGACTCCCTTGGAATGGTTGTCTATGGAGAGC
AAAACACCCTCAACGTTACAATGGATCAGATGATTTTCCATACTAGGGCAGTTGCCAAAGCCGTGAAGAGGGCATTGGTT
TTGGCAGACATGCCCTTCGGCAGCTATGAAGTTTCTGTTGAAGAGGGAGTGAAGAACGCCATAAAGCTAATTCAGGCCGG
AGCAGATGCAGTTAAGATAGAGGGGGGCTATGACCATAGAAAACTTGTCAAAAAGCTGGTAAGGATGGGAATCCCAGTTA
TGGGCCACACAGGGCTTACTCCCCAGAGATACCTAAGGCTCGGTGGTTACAGAATAATGGGAGAGAATGAAGAGGAAGTT
GAGGAAATATTGAGGGATGCAAAAGCTTTGGAAAAAGCTGGGGCCTTTGCCGTCGTTCTGGAATTTGTCCTAGCCGACGT
GGCAAAGCTAGTTACTGAAGAGGTCTCAATCCCCACCATAGGAATTGGCTCTGGACCCTACGTTGATGGCCAAGTTTTGG
TTTGGCACGATGTTTTGGGGCTATATGAAAGTTCACCCCCCTTCGCCAAGAGGTATGCGAACCTTAGAGAAGAAATACTG
AGGGCAATATCGGAGTTTAGAAAAGAGGTTAAAGAAGGAAAATTCCCAGGAAAGGAGCACTACTGGGAATACCAAGATAA
AGAAACATTTAATAGGATTAAAGAGAACGTCATGAGAAAGCTTAGGCTTTAG

Upstream 100 bases:

>100_bases
ATGCAGAGGGCAATGATGGAGCAAGTCACTCCAAAGTTGCCTCATGAAGAGGAAGACAGGGGATACCTCTAAATTTTCTC
TTTTTTTCTAGGTGTTGGAG

Downstream 100 bases:

>100_bases
TTAGGCCCAAGGTTCCTAAAGTTATTAGGACAAACCCAGCAACATGCCAGAGAGTTACAGGCGTTCCGGTAAGTAGAGCT
ATTCCTATGGCAACTGCTGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MREITPKRIMEMKGKEKITMITAYDYPSALLADKAGFDIVFVGDSLGMVVYGEQNTLNVTMDQMIFHTRAVAKAVKRALV
LADMPFGSYEVSVEEGVKNAIKLIQAGADAVKIEGGYDHRKLVKKLVRMGIPVMGHTGLTPQRYLRLGGYRIMGENEEEV
EEILRDAKALEKAGAFAVVLEFVLADVAKLVTEEVSIPTIGIGSGPYVDGQVLVWHDVLGLYESSPPFAKRYANLREEIL
RAISEFRKEVKEGKFPGKEHYWEYQDKETFNRIKENVMRKLRL

Sequences:

>Translated_283_residues
MREITPKRIMEMKGKEKITMITAYDYPSALLADKAGFDIVFVGDSLGMVVYGEQNTLNVTMDQMIFHTRAVAKAVKRALV
LADMPFGSYEVSVEEGVKNAIKLIQAGADAVKIEGGYDHRKLVKKLVRMGIPVMGHTGLTPQRYLRLGGYRIMGENEEEV
EEILRDAKALEKAGAFAVVLEFVLADVAKLVTEEVSIPTIGIGSGPYVDGQVLVWHDVLGLYESSPPFAKRYANLREEIL
RAISEFRKEVKEGKFPGKEHYWEYQDKETFNRIKENVMRKLRL
>Mature_283_residues
MREITPKRIMEMKGKEKITMITAYDYPSALLADKAGFDIVFVGDSLGMVVYGEQNTLNVTMDQMIFHTRAVAKAVKRALV
LADMPFGSYEVSVEEGVKNAIKLIQAGADAVKIEGGYDHRKLVKKLVRMGIPVMGHTGLTPQRYLRLGGYRIMGENEEEV
EEILRDAKALEKAGAFAVVLEFVLADVAKLVTEEVSIPTIGIGSGPYVDGQVLVWHDVLGLYESSPPFAKRYANLREEIL
RAISEFRKEVKEGKFPGKEHYWEYQDKETFNRIKENVMRKLRL

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=250, Percent_Identity=42.8, Blast_Score=200, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6319653, Length=248, Percent_Identity=35.8870967741936, Blast_Score=178, Evalue=1e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PYRFU (Q8U1R2)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578872.1
- ProteinModelPortal:   Q8U1R2
- SMR:   Q8U1R2
- EnsemblBacteria:   EBPYRT00000005356
- GeneID:   1469012
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1143
- NMPDR:   fig|186497.1.peg.1183
- GeneTree:   EBGT00050000022489
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 31891; Mature: 31891

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: ACT_SITE 181-181 BINDING 83-83 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREITPKRIMEMKGKEKITMITAYDYPSALLADKAGFDIVFVGDSLGMVVYGEQNTLNVT
CCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCEEEEECCCCCEEEECCCCCEEHH
MDQMIFHTRAVAKAVKRALVLADMPFGSYEVSVEEGVKNAIKLIQAGADAVKIEGGYDHR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCHH
KLVKKLVRMGIPVMGHTGLTPQRYLRLGGYRIMGENEEEVEEILRDAKALEKAGAFAVVL
HHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCHHHHHH
EFVLADVAKLVTEEVSIPTIGIGSGPYVDGQVLVWHDVLGLYESSPPFAKRYANLREEIL
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHH
RAISEFRKEVKEGKFPGKEHYWEYQDKETFNRIKENVMRKLRL
HHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MREITPKRIMEMKGKEKITMITAYDYPSALLADKAGFDIVFVGDSLGMVVYGEQNTLNVT
CCCCCHHHHHHHCCCCEEEEEEECCCCHHHHHHCCCCEEEEECCCCCEEEECCCCCEEHH
MDQMIFHTRAVAKAVKRALVLADMPFGSYEVSVEEGVKNAIKLIQAGADAVKIEGGYDHR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCCCCHH
KLVKKLVRMGIPVMGHTGLTPQRYLRLGGYRIMGENEEEVEEILRDAKALEKAGAFAVVL
HHHHHHHHHCCCCCCCCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHCHHHHHH
EFVLADVAKLVTEEVSIPTIGIGSGPYVDGQVLVWHDVLGLYESSPPFAKRYANLREEIL
HHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHH
RAISEFRKEVKEGKFPGKEHYWEYQDKETFNRIKENVMRKLRL
HHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA