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The map label for this gene is asnB [C]

Identifier: 18977443

GI number: 18977443

Start: 1021338

End: 1022753

Strand: Direct

Name: asnB [C]

Synonym: PF1071

Alternate gene names: 18977443

Gene position: 1021338-1022753 (Clockwise)

Preceding gene: 18977442

Following gene: 18977444

Centisome position: 53.52

GC content: 41.38

Gene sequence:

>1416_bases
ATGTGTCTAATAACCGGAGGAATAAATCTAACAAAGGAAGAAATCGTCAAGATGATAATTACTGGAAAGCATAGAGGACC
AGATTCTTTTGGCGTATGGAGCGATGGGTTAGTTATAAAATCTGAGGACTTCTCAGAGATAAAAGAAATTAGAGGGGGCA
ACGTTGTTTTAGCCCAGTGTAGGCTTGCAATGACTGGATCTAAAAACTATACCCAACCCTTTTACAATGACATTGTTCTC
GTCCACAATGGAGAGATATATAACCACGAAAGTTTGAGGAAGTGGTTAATAGAGAGGGGAGCATCATTTGAGAGTGACGT
GGACAGTGAGGTTATCCTAAGACTTTTGGAGTATTTTATATTTGACAAAAAGATGAAAGTTGAGGAGGCCGTTAAAAAAG
CGATGCTTATGTTGAGGGGAGACTACGCCGTCGCATTTGCTCTCAATGGTAAAATCTATTTGTTTAGGGATCCTATTGGA
GTTAGACCACTCTACTATTCCCCAAGGGGGAGGTTTGGGTCAGAGAAGAAGGTGTTGTGGAGTGTAGGTGAAGAAGCAAT
ACCAGTTAACCCAGGTGAGCTCGTTAAGTTGCCAGAAGAAGAGAAGATGAAGCTACTTTCCCCCTGGGACATTAAAGGTT
CTGGAAAAGGATACTACGACGGTCTTAAGAATTTACTTCAAGATGCTGTAAGAATAAGGACAGCTAAAAAAGTTGGAGTG
CTCTTTTCAGGAGGATTGGACAGCTCATTAATAGCTTTGTTAGCTTCAAAATACTCTAAGGTAGTTCTCTACACTGCGGG
AACGGAGGATAGTAAAGACGTTGAGTGGGCCAGAAAAGTTAGTGATGAGCTTAGACTTGAGCTTAGAGAGTATATATTTA
CCCGGGAGGAAGTTGAGAGGGAGATTAAGAATGTAATGTTCGCTATAGAGGAGCCAAATCCAATGAACTTAGCCATTGCA
ATTCCTCTATATTTTGCCACAAAGCTTGCTAGGAAGGATGATGTTAAGGTCCTTTTGAGTGGCCAGGGTGCTGATGAACT
GTTTGGAGGATATGCAAAGTATCTCGAGAATCCCTCTTTGATGAGAAAAGATATAGAGGAGATAGCCGAGAGAAACTTAG
GTAGAGATGATAAAGTGGGAATGCTAAATGGTGTGGAAGTTAGATACCCCTACTTGGATTTGCCCTTTGTCTCTCTAGCC
TTAAGGGTTCCCCTAGAAGAAAAGATAAGAGAGGGAGTTAGAAAGGCAATTTTGAGGAGAATTGCACTAGATTTTGGCTT
GCCAGAAGAAGTTGCCTTTAGGGAGAAAAAGGCCGCACAATACGGGAGCAATGCTCAGAAGATACTCTCTAAGATAGCAA
AGAAGAGTCTTAGAGAATTCGCCCAGAAGTTATTTTCTGAGGTATTCTCTACATAA

Upstream 100 bases:

>100_bases
ATAGTTGTTGAATAGGCATTCATTGTATTTTACACCCGTTAAATACTATCCCACATGGAAAATGCAAGAGTTTTTACGTC
CTCTTGAAAAGTAAGATCCG

Downstream 100 bases:

>100_bases
TTGCACAATTAAATAATTCAACAATTATTGTATTCTGTAATTACACAGACAGATAAATTTTTATATACTTTTCACCCTAA
TTTAGTACAGAAAAATGTAC

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MCLITGGINLTKEEIVKMIITGKHRGPDSFGVWSDGLVIKSEDFSEIKEIRGGNVVLAQCRLAMTGSKNYTQPFYNDIVL
VHNGEIYNHESLRKWLIERGASFESDVDSEVILRLLEYFIFDKKMKVEEAVKKAMLMLRGDYAVAFALNGKIYLFRDPIG
VRPLYYSPRGRFGSEKKVLWSVGEEAIPVNPGELVKLPEEEKMKLLSPWDIKGSGKGYYDGLKNLLQDAVRIRTAKKVGV
LFSGGLDSSLIALLASKYSKVVLYTAGTEDSKDVEWARKVSDELRLELREYIFTREEVEREIKNVMFAIEEPNPMNLAIA
IPLYFATKLARKDDVKVLLSGQGADELFGGYAKYLENPSLMRKDIEEIAERNLGRDDKVGMLNGVEVRYPYLDLPFVSLA
LRVPLEEKIREGVRKAILRRIALDFGLPEEVAFREKKAAQYGSNAQKILSKIAKKSLREFAQKLFSEVFST

Sequences:

>Translated_471_residues
MCLITGGINLTKEEIVKMIITGKHRGPDSFGVWSDGLVIKSEDFSEIKEIRGGNVVLAQCRLAMTGSKNYTQPFYNDIVL
VHNGEIYNHESLRKWLIERGASFESDVDSEVILRLLEYFIFDKKMKVEEAVKKAMLMLRGDYAVAFALNGKIYLFRDPIG
VRPLYYSPRGRFGSEKKVLWSVGEEAIPVNPGELVKLPEEEKMKLLSPWDIKGSGKGYYDGLKNLLQDAVRIRTAKKVGV
LFSGGLDSSLIALLASKYSKVVLYTAGTEDSKDVEWARKVSDELRLELREYIFTREEVEREIKNVMFAIEEPNPMNLAIA
IPLYFATKLARKDDVKVLLSGQGADELFGGYAKYLENPSLMRKDIEEIAERNLGRDDKVGMLNGVEVRYPYLDLPFVSLA
LRVPLEEKIREGVRKAILRRIALDFGLPEEVAFREKKAAQYGSNAQKILSKIAKKSLREFAQKLFSEVFST
>Mature_471_residues
MCLITGGINLTKEEIVKMIITGKHRGPDSFGVWSDGLVIKSEDFSEIKEIRGGNVVLAQCRLAMTGSKNYTQPFYNDIVL
VHNGEIYNHESLRKWLIERGASFESDVDSEVILRLLEYFIFDKKMKVEEAVKKAMLMLRGDYAVAFALNGKIYLFRDPIG
VRPLYYSPRGRFGSEKKVLWSVGEEAIPVNPGELVKLPEEEKMKLLSPWDIKGSGKGYYDGLKNLLQDAVRIRTAKKVGV
LFSGGLDSSLIALLASKYSKVVLYTAGTEDSKDVEWARKVSDELRLELREYIFTREEVEREIKNVMFAIEEPNPMNLAIA
IPLYFATKLARKDDVKVLLSGQGADELFGGYAKYLENPSLMRKDIEEIAERNLGRDDKVGMLNGVEVRYPYLDLPFVSLA
LRVPLEEKIREGVRKAILRRIALDFGLPEEVAFREKKAAQYGSNAQKILSKIAKKSLREFAQKLFSEVFST

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=481, Percent_Identity=29.1060291060291, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI168229252, Length=481, Percent_Identity=29.1060291060291, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI168229250, Length=481, Percent_Identity=29.1060291060291, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI296010848, Length=481, Percent_Identity=29.1060291060291, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI296010852, Length=397, Percent_Identity=29.7229219143577, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI296010850, Length=397, Percent_Identity=29.7229219143577, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1786889, Length=496, Percent_Identity=27.8225806451613, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17560178, Length=432, Percent_Identity=30.0925925925926, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI25147557, Length=496, Percent_Identity=29.4354838709677, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=496, Percent_Identity=29.4354838709677, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25147560, Length=295, Percent_Identity=29.1525423728814, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321563, Length=515, Percent_Identity=28.5436893203883, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6325403, Length=485, Percent_Identity=29.0721649484536, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323542, Length=443, Percent_Identity=28.4424379232506, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI45553209, Length=459, Percent_Identity=27.8867102396514, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI221329991, Length=572, Percent_Identity=23.4265734265734, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 53351; Mature: 53351

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLITGGINLTKEEIVKMIITGKHRGPDSFGVWSDGLVIKSEDFSEIKEIRGGNVVLAQC
CEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCEEEEEE
RLAMTGSKNYTQPFYNDIVLVHNGEIYNHESLRKWLIERGASFESDVDSEVILRLLEYFI
EEEECCCCCCCCCHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FDKKMKVEEAVKKAMLMLRGDYAVAFALNGKIYLFRDPIGVRPLYYSPRGRFGSEKKVLW
HCCCHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECCCCCCCEEECCCCCCCCCCHHHH
SVGEEAIPVNPGELVKLPEEEKMKLLSPWDIKGSGKGYYDGLKNLLQDAVRIRTAKKVGV
HCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCE
LFSGGLDSSLIALLASKYSKVVLYTAGTEDSKDVEWARKVSDELRLELREYIFTREEVER
EEECCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKNVMFAIEEPNPMNLAIAIPLYFATKLARKDDVKVLLSGQGADELFGGYAKYLENPSL
HHHHHEEEECCCCCCEEEEEEHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCHHH
MRKDIEEIAERNLGRDDKVGMLNGVEVRYPYLDLPFVSLALRVPLEEKIREGVRKAILRR
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
IALDFGLPEEVAFREKKAAQYGSNAQKILSKIAKKSLREFAQKLFSEVFST
HHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCLITGGINLTKEEIVKMIITGKHRGPDSFGVWSDGLVIKSEDFSEIKEIRGGNVVLAQC
CEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCEEEEEE
RLAMTGSKNYTQPFYNDIVLVHNGEIYNHESLRKWLIERGASFESDVDSEVILRLLEYFI
EEEECCCCCCCCCHHCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
FDKKMKVEEAVKKAMLMLRGDYAVAFALNGKIYLFRDPIGVRPLYYSPRGRFGSEKKVLW
HCCCHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEECCCCCCCEEECCCCCCCCCCHHHH
SVGEEAIPVNPGELVKLPEEEKMKLLSPWDIKGSGKGYYDGLKNLLQDAVRIRTAKKVGV
HCCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCE
LFSGGLDSSLIALLASKYSKVVLYTAGTEDSKDVEWARKVSDELRLELREYIFTREEVER
EEECCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIKNVMFAIEEPNPMNLAIAIPLYFATKLARKDDVKVLLSGQGADELFGGYAKYLENPSL
HHHHHEEEECCCCCCEEEEEEHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCHHH
MRKDIEEIAERNLGRDDKVGMLNGVEVRYPYLDLPFVSLALRVPLEEKIREGVRKAILRR
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
IALDFGLPEEVAFREKKAAQYGSNAQKILSKIAKKSLREFAQKLFSEVFST
HHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]