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The map label for this gene is pgk

Identifier: 18977429

GI number: 18977429

Start: 1011463

End: 1012695

Strand: Direct

Name: pgk

Synonym: PF1057

Alternate gene names: 18977429

Gene position: 1011463-1012695 (Clockwise)

Preceding gene: 18977428

Following gene: 18977430

Centisome position: 53.0

GC content: 40.71

Gene sequence:

>1233_bases
ATGTTCAGGTTGAGAGATTTTGAATACTACAACAGAACCGTGTTTCTTCGAGTTGACTTAAACTCACCAATGAGTAATGG
AAAAATAATAAGTGACGCGAGATTTCGAGCAGTCCTTCCAACGATAAAGTACCTAATAGAGAGTGGAGCAAAAGTAGTTG
TTGGCACTCATCAAGGAAAACCCTACAGCGAAGAGTATTCGACTACTGAAGAGCATGCGAGAATTCTCTCTGAGCTCCTA
AATATGCATGTTGAATACGTTGAAGACATTTTTGGAAAGTATGCAAGGGAGAGAATAAAGGCTATGAAACCTGGGGAAGT
TATAGTCCTCGAAAACCTCAGGTTCTCTGCAGAAGAAGTAAAAAACGCAACTATTGAGGAATGCGAAAAGACGTTTTTTG
TTAGGAAGCTCTCCCAGGTAATAGACCTCGTTGTTAACGATGCCTTTGCTGCCGCTCATAGAAGTCAGCCATCTCTCGTT
GGTTTTGCAAGAATAAAGCCTATGATAATGGGATTCCTTATGGAAAAAGAAGTTGATGCTTTAACAAAAGCTTATGAAAG
TGAAGAAAAGCCGAGAGTTTATGTTCTTGGTGGTGCAAAAGTTGATGACTCATTAAAAGTTGCCGAAAACGTTCTTAGGA
AAGAAAAGGCTGATCTCATACTGACAGGGGGATTGGTTGGCCAATTATTCACTCTAGCTAAGGGCTTTGACCTGGGAAGA
GAAAACATTAAATTCTTAGAAAAGAAGGGAATTTTAAAATACGTAGACTGGGCAGAAAAGATTCTTGACGAATTTTATCC
CTACGTTAGAACTCCCGTAGACTTTGCCATTGACTTCAAGGGAGAAAGAGTAGAGATAGATTTGCTCAGCGACGAGAAAA
GGCTCTTTGACGAATATCCAATCCTTGACATCGGTTCTAGAACAGTTGAAAAGTATAGGGAGATACTCTTAAAGGCCAGG
ATAATAGTAGCCAACGGTCCTATGGGAGTGTTCGAAAGGGAAGAATTTGCCGTGGGAACAATTGGGGTATTCAAGGCCAT
CGGAGAGAGTCCAGCCTTTAGCGTCATTGGTGGTGGTCATTCAATAGCAAGCATATACAAGTACAACATAACTGGGATAA
GCCATATCTCTACCGGTGGGGGAGCTATGCTAACATTTTTTGCTGGAGAAAAGTTGCCAGTTCTTGAAGCGCTAAAGATT
AGTTACGAAAAGTTCTCTAACTTACTTTCTTAA

Upstream 100 bases:

>100_bases
ACAGCCCTTCTAAAACAACATGTTTTAGGCTATACAAATCTTTTCTTCCCCTTCCCCACAACTTTCTTAAATCTCCATCC
AACTATTTTATGGTGGTGAA

Downstream 100 bases:

>100_bases
TTTTTAACCAGACATTTAAAAGTAGAATCCTGTGAATATCCTTGGAGGTGAGGAAGTGAAAGGGGTTATTCTAGCTGCTG
GATATGGGAGTAGAATGGGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MFRLRDFEYYNRTVFLRVDLNSPMSNGKIISDARFRAVLPTIKYLIESGAKVVVGTHQGKPYSEEYSTTEEHARILSELL
NMHVEYVEDIFGKYARERIKAMKPGEVIVLENLRFSAEEVKNATIEECEKTFFVRKLSQVIDLVVNDAFAAAHRSQPSLV
GFARIKPMIMGFLMEKEVDALTKAYESEEKPRVYVLGGAKVDDSLKVAENVLRKEKADLILTGGLVGQLFTLAKGFDLGR
ENIKFLEKKGILKYVDWAEKILDEFYPYVRTPVDFAIDFKGERVEIDLLSDEKRLFDEYPILDIGSRTVEKYREILLKAR
IIVANGPMGVFEREEFAVGTIGVFKAIGESPAFSVIGGGHSIASIYKYNITGISHISTGGGAMLTFFAGEKLPVLEALKI
SYEKFSNLLS

Sequences:

>Translated_410_residues
MFRLRDFEYYNRTVFLRVDLNSPMSNGKIISDARFRAVLPTIKYLIESGAKVVVGTHQGKPYSEEYSTTEEHARILSELL
NMHVEYVEDIFGKYARERIKAMKPGEVIVLENLRFSAEEVKNATIEECEKTFFVRKLSQVIDLVVNDAFAAAHRSQPSLV
GFARIKPMIMGFLMEKEVDALTKAYESEEKPRVYVLGGAKVDDSLKVAENVLRKEKADLILTGGLVGQLFTLAKGFDLGR
ENIKFLEKKGILKYVDWAEKILDEFYPYVRTPVDFAIDFKGERVEIDLLSDEKRLFDEYPILDIGSRTVEKYREILLKAR
IIVANGPMGVFEREEFAVGTIGVFKAIGESPAFSVIGGGHSIASIYKYNITGISHISTGGGAMLTFFAGEKLPVLEALKI
SYEKFSNLLS
>Mature_410_residues
MFRLRDFEYYNRTVFLRVDLNSPMSNGKIISDARFRAVLPTIKYLIESGAKVVVGTHQGKPYSEEYSTTEEHARILSELL
NMHVEYVEDIFGKYARERIKAMKPGEVIVLENLRFSAEEVKNATIEECEKTFFVRKLSQVIDLVVNDAFAAAHRSQPSLV
GFARIKPMIMGFLMEKEVDALTKAYESEEKPRVYVLGGAKVDDSLKVAENVLRKEKADLILTGGLVGQLFTLAKGFDLGR
ENIKFLEKKGILKYVDWAEKILDEFYPYVRTPVDFAIDFKGERVEIDLLSDEKRLFDEYPILDIGSRTVEKYREILLKAR
IIVANGPMGVFEREEFAVGTIGVFKAIGESPAFSVIGGGHSIASIYKYNITGISHISTGGGAMLTFFAGEKLPVLEALKI
SYEKFSNLLS

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=32.5301204819277, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=33.2530120481928, Blast_Score=193, Evalue=3e-49,
Organism=Escherichia coli, GI1789294, Length=406, Percent_Identity=35.7142857142857, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17508823, Length=410, Percent_Identity=31.9512195121951, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319857, Length=409, Percent_Identity=35.4523227383863, Blast_Score=202, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=30.6763285024155, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17136394, Length=418, Percent_Identity=31.5789473684211, Blast_Score=174, Evalue=9e-44,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_PYRFU (P61883)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578786.1
- ProteinModelPortal:   P61883
- SMR:   P61883
- EnsemblBacteria:   EBPYRT00000004157
- GeneID:   1468923
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1057
- NMPDR:   fig|186497.1.peg.1094
- GeneTree:   EBGT00050000022333
- HOGENOM:   HBG453500
- OMA:   PGKKDFT
- ProtClustDB:   PRK00073
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 46225; Mature: 46225

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 34-34 BINDING 114-114 BINDING 154-154 BINDING 332-332

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRLRDFEYYNRTVFLRVDLNSPMSNGKIISDARFRAVLPTIKYLIESGAKVVVGTHQGK
CCCCCCCHHHCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCC
PYSEEYSTTEEHARILSELLNMHVEYVEDIFGKYARERIKAMKPGEVIVLENLRFSAEEV
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHH
KNATIEECEKTFFVRKLSQVIDLVVNDAFAAAHRSQPSLVGFARIKPMIMGFLMEKEVDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
LTKAYESEEKPRVYVLGGAKVDDSLKVAENVLRKEKADLILTGGLVGQLFTLAKGFDLGR
HHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCH
ENIKFLEKKGILKYVDWAEKILDEFYPYVRTPVDFAIDFKGERVEIDLLSDEKRLFDEYP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCHHHHHHCCC
ILDIGSRTVEKYREILLKARIIVANGPMGVFEREEFAVGTIGVFKAIGESPAFSVIGGGH
CEECCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEHHHHHHHHHCCCCCEEEECCCC
SIASIYKYNITGISHISTGGGAMLTFFAGEKLPVLEALKISYEKFSNLLS
CEEHEEEEECCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFRLRDFEYYNRTVFLRVDLNSPMSNGKIISDARFRAVLPTIKYLIESGAKVVVGTHQGK
CCCCCCCHHHCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCC
PYSEEYSTTEEHARILSELLNMHVEYVEDIFGKYARERIKAMKPGEVIVLENLRFSAEEV
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHH
KNATIEECEKTFFVRKLSQVIDLVVNDAFAAAHRSQPSLVGFARIKPMIMGFLMEKEVDA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHH
LTKAYESEEKPRVYVLGGAKVDDSLKVAENVLRKEKADLILTGGLVGQLFTLAKGFDLGR
HHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCCH
ENIKFLEKKGILKYVDWAEKILDEFYPYVRTPVDFAIDFKGERVEIDLLSDEKRLFDEYP
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCHHHHHHCCC
ILDIGSRTVEKYREILLKARIIVANGPMGVFEREEFAVGTIGVFKAIGESPAFSVIGGGH
CEECCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEHHHHHHHHHCCCCCEEEECCCC
SIASIYKYNITGISHISTGGGAMLTFFAGEKLPVLEALKISYEKFSNLLS
CEEHEEEEECCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA