Click here to switch to the map view.

The map label for this gene is thrA [H]

Identifier: 18977424

GI number: 18977424

Start: 1006040

End: 1007161

Strand: Direct

Name: thrA [H]

Synonym: PF1052

Alternate gene names: 18977424

Gene position: 1006040-1007161 (Clockwise)

Preceding gene: 18977423

Following gene: 161484711

Centisome position: 52.72

GC content: 39.84

Gene sequence:

>1122_bases
ATGATCGTGCTAAAATTTGGGGGAAGCTCAATTAAGTATGACTTCGAGGAAGCAACAAATTTAGCCCTCAGTCTCTTTGA
AGATGATGCTGTCATTGTAGTAGTTTCTGCAATAAAAGGGATAACAGATAAGCTGATTCTGTACTCCCAGACCTTGGATA
AGACAATAGCAACAAAAATAGCTTCTGAGTATGTTAGCTTTGCAAAATCCCATGGTATAGATCCAGTGATATTGAAACCC
TACTTAGATGAGCTTTTTAACCTTCCAGACCTTCCAGAGGAGGCACTAAGGGATTATATCCTGTCTTTGGGCGAAGTTCT
TTCTGCTGTAATTTTCTCAGCCTCAATTGGAGGAAAATTCGTCCCCTCTTGGGATATTTTTGAGGCCGAGGGTAACTTTG
GAAATGCATTCATAGACATAAAAAGTAGCAAGAAAAAATTCAAGAGAGTATTTGAGGTGCTTGAAGAAGGCTATATTCCA
GTTATCCCAGGATTTACGGCTGGAAAAGATGGCTACATTCTCACCCTGGGAAGAGGGGGGAGTGACTATTCGGGAGTTGC
CGCTGGAGTCCTTGGAAAGGCAAAATTAGTGGCGATTATGAGTGATGTCGAGGGAATTTATACAGCAGATCCAAAGCTAG
TCCCCTCGGCAAGGTTAATTCCCTACGTTTCTTATGATGAGATCATAATATCCTCAAAACATGGCATGAGGGCTTTACAA
TGGAAAGCTGCAGAATTAGCTAAAGAGTATAAGATTCCAGTCCTCTTTGGAAGGACTAGGAACTGGAGAATGGGAACTTT
GATGAGCGAAAGGAGCTCAGGAATGCCGCTTATGACATACAAAGATGGGCTGCTACTTGTTAATGTAACTGAAGAGATAA
AATATCCGATTATAGCTGAAGGGCCTTTCTGGAAACTCTACAAAGTTTCCCAAGAAGAGGGAATTCACTTAATGAGAGAG
CTTCATACAAAATTATTTCAACCGTCATTTATCCAATATAGTGTTTACAAATCTAGTGAAAATGGGCAAAAAACTCAAGA
ATTTAACGAAAAAATATCAATTGAACAAGGAGAAAATCGAAAAATTTATTACAGTGAGGAAGAGGTTAACCTTCCTGGGT
GA

Upstream 100 bases:

>100_bases
ATATTACTGCTGGTTATTATTAGTTGATGAAAATATGTCGAAAGATATATAAAATTGAAGACAAAATTGGGGTGGAGAGA
AAATAGGAGGAGGGAGAGAG

Downstream 100 bases:

>100_bases
ACGACCATCGGTGGTGGAGAAACCTGTTGTTGTAAAGTTTGGAGGAACTTCAGTAAGGTACGCCTTTGAAGAAGCACTAG
AGCTAGTTGCAAATCTAAAC

Product: aspartate kinase

Products: NA

Alternate protein names: AK-HD; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIVLKFGGSSIKYDFEEATNLALSLFEDDAVIVVVSAIKGITDKLILYSQTLDKTIATKIASEYVSFAKSHGIDPVILKP
YLDELFNLPDLPEEALRDYILSLGEVLSAVIFSASIGGKFVPSWDIFEAEGNFGNAFIDIKSSKKKFKRVFEVLEEGYIP
VIPGFTAGKDGYILTLGRGGSDYSGVAAGVLGKAKLVAIMSDVEGIYTADPKLVPSARLIPYVSYDEIIISSKHGMRALQ
WKAAELAKEYKIPVLFGRTRNWRMGTLMSERSSGMPLMTYKDGLLLVNVTEEIKYPIIAEGPFWKLYKVSQEEGIHLMRE
LHTKLFQPSFIQYSVYKSSENGQKTQEFNEKISIEQGENRKIYYSEEEVNLPG

Sequences:

>Translated_373_residues
MIVLKFGGSSIKYDFEEATNLALSLFEDDAVIVVVSAIKGITDKLILYSQTLDKTIATKIASEYVSFAKSHGIDPVILKP
YLDELFNLPDLPEEALRDYILSLGEVLSAVIFSASIGGKFVPSWDIFEAEGNFGNAFIDIKSSKKKFKRVFEVLEEGYIP
VIPGFTAGKDGYILTLGRGGSDYSGVAAGVLGKAKLVAIMSDVEGIYTADPKLVPSARLIPYVSYDEIIISSKHGMRALQ
WKAAELAKEYKIPVLFGRTRNWRMGTLMSERSSGMPLMTYKDGLLLVNVTEEIKYPIIAEGPFWKLYKVSQEEGIHLMRE
LHTKLFQPSFIQYSVYKSSENGQKTQEFNEKISIEQGENRKIYYSEEEVNLPG
>Mature_373_residues
MIVLKFGGSSIKYDFEEATNLALSLFEDDAVIVVVSAIKGITDKLILYSQTLDKTIATKIASEYVSFAKSHGIDPVILKP
YLDELFNLPDLPEEALRDYILSLGEVLSAVIFSASIGGKFVPSWDIFEAEGNFGNAFIDIKSSKKKFKRVFEVLEEGYIP
VIPGFTAGKDGYILTLGRGGSDYSGVAAGVLGKAKLVAIMSDVEGIYTADPKLVPSARLIPYVSYDEIIISSKHGMRALQ
WKAAELAKEYKIPVLFGRTRNWRMGTLMSERSSGMPLMTYKDGLLLVNVTEEIKYPIIAEGPFWKLYKVSQEEGIHLMRE
LHTKLFQPSFIQYSVYKSSENGQKTQEFNEKISIEQGENRKIYYSEEEVNLPG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=301, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790455, Length=297, Percent_Identity=28.956228956229, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790376, Length=278, Percent_Identity=29.8561151079137, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 41629; Mature: 41629

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLKFGGSSIKYDFEEATNLALSLFEDDAVIVVVSAIKGITDKLILYSQTLDKTIATKI
CEEEEECCCCEEECHHHHCCCEEEEECCCCEEEEEEHHHCCHHHHEEEHHHHHHHHHHHH
ASEYVSFAKSHGIDPVILKPYLDELFNLPDLPEEALRDYILSLGEVLSAVIFSASIGGKF
HHHHHHHHHHCCCCCEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPSWDIFEAEGNFGNAFIDIKSSKKKFKRVFEVLEEGYIPVIPGFTAGKDGYILTLGRGG
CCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCC
SDYSGVAAGVLGKAKLVAIMSDVEGIYTADPKLVPSARLIPYVSYDEIIISSKHGMRALQ
CCCCCHHHHHHCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCEEEECCCCCHHHH
WKAAELAKEYKIPVLFGRTRNWRMGTLMSERSSGMPLMTYKDGLLLVNVTEEIKYPIIAE
HHHHHHHHHCCCEEEEECCCCCEEHHHHHHHCCCCEEEEECCCEEEEEEHHHCCCCEEEC
GPFWKLYKVSQEEGIHLMRELHTKLFQPSFIQYSVYKSSENGQKTQEFNEKISIEQGENR
CCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHEECCCCCCCC
KIYYSEEEVNLPG
EEEECCCCCCCCC
>Mature Secondary Structure
MIVLKFGGSSIKYDFEEATNLALSLFEDDAVIVVVSAIKGITDKLILYSQTLDKTIATKI
CEEEEECCCCEEECHHHHCCCEEEEECCCCEEEEEEHHHCCHHHHEEEHHHHHHHHHHHH
ASEYVSFAKSHGIDPVILKPYLDELFNLPDLPEEALRDYILSLGEVLSAVIFSASIGGKF
HHHHHHHHHHCCCCCEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VPSWDIFEAEGNFGNAFIDIKSSKKKFKRVFEVLEEGYIPVIPGFTAGKDGYILTLGRGG
CCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCC
SDYSGVAAGVLGKAKLVAIMSDVEGIYTADPKLVPSARLIPYVSYDEIIISSKHGMRALQ
CCCCCHHHHHHCHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCCEEEECCCCCHHHH
WKAAELAKEYKIPVLFGRTRNWRMGTLMSERSSGMPLMTYKDGLLLVNVTEEIKYPIIAE
HHHHHHHHHCCCEEEEECCCCCEEHHHHHHHCCCCEEEEECCCEEEEEEHHHCCCCEEEC
GPFWKLYKVSQEEGIHLMRELHTKLFQPSFIQYSVYKSSENGQKTQEFNEKISIEQGENR
CCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHEECCCCCCCC
KIYYSEEEVNLPG
EEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]