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The map label for this gene is pyrE

Identifier: 18977406

GI number: 18977406

Start: 990043

End: 990591

Strand: Reverse

Name: pyrE

Synonym: PF1034

Alternate gene names: 18977406

Gene position: 990591-990043 (Counterclockwise)

Preceding gene: 18977410

Following gene: 18977405

Centisome position: 51.91

GC content: 39.89

Gene sequence:

>549_bases
ATGAAGGAAGAACTAATAAGGATGATACTTGAAGAAGAATGCATAAAGTTTGGCCACTTTATTTTAACTTCAGGGAAGGA
GAGCTCCTATTACATAGATATAAAGAAGCTCATAACGAATCCCAAAGCCCTTCGATTAATAGCAAGACTAATCAAAGAGA
AGGCAGAAGAAGAAGGAATTCAGTTTGATAAAGTAGCAGGTCCAGAGCTTGGAGCCGTTCCAATTGCAACAGCCTTAGCG
TTGGAGACAGATAGGCCCTTGCTAATTGTAAGAAAGAAAAAGAAAGAACATGGAACTGGAAGGCAGATAGAAGGAGAAGT
AAAAGAAGGGGATAGGGTTCTTCTAGTTGAAGATGTAACTACAACTGGAGGAAGCGTATTAAGAGCTGCAAAAATACTGA
AAGAAGCTGGGGCAGAGATAACTGGGATATTCGTTGTGGTAGATAGAGAAGAAGGTGCCAAAGAGGCCATAGAAAAAGAA
GGATTCAAGCTTTATCCTCTAGTTTTGGTTCATGAATTATTTGAAGCTGCTGGAGTCTCTTCTGAGTGA

Upstream 100 bases:

>100_bases
AGGTCAGGTATTTATAAAGATTTATCCCAAAGCAAAGTCGAGTATAATATCTCCCATCTAAATTTTTAAAGTTAAGCATA
ACTTATTCTGAGGGTTCACT

Downstream 100 bases:

>100_bases
CTTTTCTCTTTCTTTTTGCTTTGCTACTTTCTTAATATTGTTCTCTAATTTGTATCTTATTAGGCAAACCGAAAAGTTTA
TATATTCGAACTATATTAGT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKEELIRMILEEECIKFGHFILTSGKESSYYIDIKKLITNPKALRLIARLIKEKAEEEGIQFDKVAGPELGAVPIATALA
LETDRPLLIVRKKKKEHGTGRQIEGEVKEGDRVLLVEDVTTTGGSVLRAAKILKEAGAEITGIFVVVDREEGAKEAIEKE
GFKLYPLVLVHELFEAAGVSSE

Sequences:

>Translated_182_residues
MKEELIRMILEEECIKFGHFILTSGKESSYYIDIKKLITNPKALRLIARLIKEKAEEEGIQFDKVAGPELGAVPIATALA
LETDRPLLIVRKKKKEHGTGRQIEGEVKEGDRVLLVEDVTTTGGSVLRAAKILKEAGAEITGIFVVVDREEGAKEAIEKE
GFKLYPLVLVHELFEAAGVSSE
>Mature_182_residues
MKEELIRMILEEECIKFGHFILTSGKESSYYIDIKKLITNPKALRLIARLIKEKAEEEGIQFDKVAGPELGAVPIATALA
LETDRPLLIVRKKKKEHGTGRQIEGEVKEGDRVLLVEDVTTTGGSVLRAAKILKEAGAEITGIFVVVDREEGAKEAIEKE
GFKLYPLVLVHELFEAAGVSSE

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=171, Percent_Identity=34.5029239766082, Blast_Score=130, Evalue=7e-31,
Organism=Escherichia coli, GI1790073, Length=156, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17554964, Length=169, Percent_Identity=37.2781065088757, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17508631, Length=167, Percent_Identity=31.1377245508982, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323530, Length=159, Percent_Identity=26.4150943396226, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323927, Length=159, Percent_Identity=28.3018867924528, Blast_Score=71, Evalue=9e-14,
Organism=Drosophila melanogaster, GI17933654, Length=172, Percent_Identity=32.5581395348837, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_PYRFU (P58861)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578763.1
- ProteinModelPortal:   P58861
- SMR:   P58861
- EnsemblBacteria:   EBPYRT00000005562
- GeneID:   1468900
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF1034
- NMPDR:   fig|186497.1.peg.1071
- GeneTree:   EBGT00050000023182
- HOGENOM:   HBG404341
- OMA:   GKEADYY
- ProtClustDB:   PRK00455
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 20099; Mature: 20099

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 91-91 BINDING 92-92 BINDING 95-95 BINDING 97-97 BINDING 121-121 BINDING 149-149

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEELIRMILEEECIKFGHFILTSGKESSYYIDIKKLITNPKALRLIARLIKEKAEEEGI
CHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCC
QFDKVAGPELGAVPIATALALETDRPLLIVRKKKKEHGTGRQIEGEVKEGDRVLLVEDVT
EEECCCCCCCCCHHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECC
TTGGSVLRAAKILKEAGAEITGIFVVVDREEGAKEAIEKEGFKLYPLVLVHELFEAAGVS
CCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCEEHHHHHHHHHHHHCCCC
SE
CC
>Mature Secondary Structure
MKEELIRMILEEECIKFGHFILTSGKESSYYIDIKKLITNPKALRLIARLIKEKAEEEGI
CHHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHCCC
QFDKVAGPELGAVPIATALALETDRPLLIVRKKKKEHGTGRQIEGEVKEGDRVLLVEDVT
EEECCCCCCCCCHHHHHHHEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECC
TTGGSVLRAAKILKEAGAEITGIFVVVDREEGAKEAIEKEGFKLYPLVLVHELFEAAGVS
CCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCEEHHHHHHHHHHHHCCCC
SE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA