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The map label for this gene is pheT

Identifier: 18977362

GI number: 18977362

Start: 944277

End: 945947

Strand: Direct

Name: pheT

Synonym: PF0990

Alternate gene names: 18977362

Gene position: 944277-945947 (Clockwise)

Preceding gene: 18977361

Following gene: 18977369

Centisome position: 49.48

GC content: 40.57

Gene sequence:

>1671_bases
ATGCCAAAGTTTGACGTTGCAAAATCAGACTTGGAAAGATTAGTTGGAAGAGAGTTTTCAGTTGAAGAGTGGGAAGACCT
TGTTCTTTATGCTAAGTGCGAGCTCGATGACGTTTGGGAAGAAGATGGAAAAATATATTTCAAGTTGGACTCAAAAGACA
CTAATAGGCCAGATCTTTGGAGCGCTGAGGGCGTTGCTCGACAAATAAGATGGGCTCTTGGATTAGCCAAGGGATTGCCA
AAATATGAAGTCGAGGAAAGTGATGTTGTTGTTTATGTTGATAAAAAGCTCAGAAACATAAGGCCCTACGGTGTTTACGC
CGTAGTAGAAGGATTAAAGCTAGATGAAGAAGCCTTGAGTCAGTTAATTCAGCTCCAAGAAAAAGTTGCCCTAACCTATG
GAAGGAGGAGAAGAGAAGTCGCAATAGGGATATTTGATTTTGACAAAGTAAAGCCCCCAATATATTACAGAGCTGCAGAA
AAGACAGAAAAATTTGTCCCCCTTGGATATAGTGAGGAAATGACGTTAGAAGAAATCCTTGAAAAGCACGAGAAAGGAAG
GGAGTATGGCCACTTAATAAGAGACAAGCCTTACTACCCACTTCTTGTTGATTCAGAAGGTAACGTCCTTTCAATGCCTC
CAATAATAAACTCCGAGCTAACGGGGAGAGTGACCACAGAGACCAAGAATGTGTTCGTGGACGTCACGGGATGGGATCTG
AGAAAAGTCATGTTAGCCCTGAATGTAATAGTCACGGCGCTCGCCGAAAGAGGAGGGAAAATCAAAAGGGTTAAAGTGAT
TTACCCTGACTTCGAGATCACGACTCCTGACTTGACTCCTAAAGAATTTGAAGTTAGTTTTGAGTACATAAGAAAGCTTT
CTGGGTTAGAGTTAAGCAATGAAGAAATCAAGGAACTTTTAGAAAGAATGATGTATGAAGTTGAAATTCTCAGTGAAAAC
AAGGCAAAAGTAAAGTATCCAGCCTTCAGAGATGACATAATGCACACTAGAGATGTACTTGAGGATGTGTTAATTGCTTA
CGGGTACAATAATATAGACCCAGAAGAGCCAAAGTTAGCTGTCCAAGGGAGAGGTGATCCATTTAAGGACTTTGAGGATG
CAATTAGGGATTTAATGGTGGGATTTGGTCTCCAGGAAGTCATGACTTTTAACTTAACAAGCAAAGAAGTCCAGTTTGAC
AAGATGAACATTCCAGAGGAGGAAATTGTTGAAATAGCAAATCCAATTAGTAGCAGATGGAGCGCATTAAGAAAATGGTT
ACTTCCAAGTTTGATGGAATTCCTTAGCAACAACACTCATGAAGAATATCCACAAAGGATATTCGAGGTTGGTTTAGCAA
CTTTGATAGACGAGAGCAGAGAAACTAAGACTGTTAGTGAACCTAAGCTTGCAGTAGCTCTAGCCGGAAGTGGATACACC
TTCACCAACGCAAAGGAAATACTTGACTCCTTAATGAGACACCTAGGAATTGAATATGATATAGAAGAGACAGTGCATGG
AAGCTTTATCCCAGGGAGAGTTGGAAAGATACTTGTGGATGGAAAGGAAATCGGAATCATAGGAGAGATTCATCCACAAG
TTCTAGAAAATTGGAATATCCAAGTTCCAGTTGTTGCTTTTGAGATATTTTTGAAGCCTCTCTATCGCTGA

Upstream 100 bases:

>100_bases
CTTGCAATGTTCAGGCTTGGAATAGATGATATTAGATATCTCTTCAGCTACGATCTGAAGTGGCTTAGGGAAGCTAAGCT
CATTTGGTAGGTGATGAGAA

Downstream 100 bases:

>100_bases
CTCTTCCTATATATTTTTTTGCACAGTTTAGCATTATTTCGAAATTTTCAAGGTTTACAGCAGTCTCTATTTCCATTCTC
TCACCAAGATGTTGCATACA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MPKFDVAKSDLERLVGREFSVEEWEDLVLYAKCELDDVWEEDGKIYFKLDSKDTNRPDLWSAEGVARQIRWALGLAKGLP
KYEVEESDVVVYVDKKLRNIRPYGVYAVVEGLKLDEEALSQLIQLQEKVALTYGRRRREVAIGIFDFDKVKPPIYYRAAE
KTEKFVPLGYSEEMTLEEILEKHEKGREYGHLIRDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETKNVFVDVTGWDL
RKVMLALNVIVTALAERGGKIKRVKVIYPDFEITTPDLTPKEFEVSFEYIRKLSGLELSNEEIKELLERMMYEVEILSEN
KAKVKYPAFRDDIMHTRDVLEDVLIAYGYNNIDPEEPKLAVQGRGDPFKDFEDAIRDLMVGFGLQEVMTFNLTSKEVQFD
KMNIPEEEIVEIANPISSRWSALRKWLLPSLMEFLSNNTHEEYPQRIFEVGLATLIDESRETKTVSEPKLAVALAGSGYT
FTNAKEILDSLMRHLGIEYDIEETVHGSFIPGRVGKILVDGKEIGIIGEIHPQVLENWNIQVPVVAFEIFLKPLYR

Sequences:

>Translated_556_residues
MPKFDVAKSDLERLVGREFSVEEWEDLVLYAKCELDDVWEEDGKIYFKLDSKDTNRPDLWSAEGVARQIRWALGLAKGLP
KYEVEESDVVVYVDKKLRNIRPYGVYAVVEGLKLDEEALSQLIQLQEKVALTYGRRRREVAIGIFDFDKVKPPIYYRAAE
KTEKFVPLGYSEEMTLEEILEKHEKGREYGHLIRDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETKNVFVDVTGWDL
RKVMLALNVIVTALAERGGKIKRVKVIYPDFEITTPDLTPKEFEVSFEYIRKLSGLELSNEEIKELLERMMYEVEILSEN
KAKVKYPAFRDDIMHTRDVLEDVLIAYGYNNIDPEEPKLAVQGRGDPFKDFEDAIRDLMVGFGLQEVMTFNLTSKEVQFD
KMNIPEEEIVEIANPISSRWSALRKWLLPSLMEFLSNNTHEEYPQRIFEVGLATLIDESRETKTVSEPKLAVALAGSGYT
FTNAKEILDSLMRHLGIEYDIEETVHGSFIPGRVGKILVDGKEIGIIGEIHPQVLENWNIQVPVVAFEIFLKPLYR
>Mature_555_residues
PKFDVAKSDLERLVGREFSVEEWEDLVLYAKCELDDVWEEDGKIYFKLDSKDTNRPDLWSAEGVARQIRWALGLAKGLPK
YEVEESDVVVYVDKKLRNIRPYGVYAVVEGLKLDEEALSQLIQLQEKVALTYGRRRREVAIGIFDFDKVKPPIYYRAAEK
TEKFVPLGYSEEMTLEEILEKHEKGREYGHLIRDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETKNVFVDVTGWDLR
KVMLALNVIVTALAERGGKIKRVKVIYPDFEITTPDLTPKEFEVSFEYIRKLSGLELSNEEIKELLERMMYEVEILSENK
AKVKYPAFRDDIMHTRDVLEDVLIAYGYNNIDPEEPKLAVQGRGDPFKDFEDAIRDLMVGFGLQEVMTFNLTSKEVQFDK
MNIPEEEIVEIANPISSRWSALRKWLLPSLMEFLSNNTHEEYPQRIFEVGLATLIDESRETKTVSEPKLAVALAGSGYTF
TNAKEILDSLMRHLGIEYDIEETVHGSFIPGRVGKILVDGKEIGIIGEIHPQVLENWNIQVPVVAFEIFLKPLYR

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B5 domain

Homologues:

Organism=Homo sapiens, GI124028525, Length=594, Percent_Identity=28.7878787878788, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI24308207, Length=165, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788006, Length=432, Percent_Identity=24.537037037037, Blast_Score=82, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17533403, Length=595, Percent_Identity=30.4201680672269, Blast_Score=258, Evalue=5e-69,
Organism=Saccharomyces cerevisiae, GI6323089, Length=600, Percent_Identity=28.5, Blast_Score=230, Evalue=4e-61,
Organism=Drosophila melanogaster, GI21355211, Length=591, Percent_Identity=29.6108291032149, Blast_Score=255, Evalue=6e-68,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_PYRFU (Q8U260)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578719.1
- ProteinModelPortal:   Q8U260
- SMR:   Q8U260
- EnsemblBacteria:   EBPYRT00000004571
- GeneID:   1468855
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0990
- NMPDR:   fig|186497.1.peg.1026
- GeneTree:   EBGT00050000023212
- HOGENOM:   HBG497667
- OMA:   LLTRMCL
- ProtClustDB:   PRK09616
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00284
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR004531
- InterPro:   IPR022918
- InterPro:   IPR002319
- Gene3D:   G3DSA:3.30.56.20
- SMART:   SM00873
- SMART:   SM00874
- TIGRFAMs:   TIGR00471

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF01409 tRNA-synt_2d; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 63964; Mature: 63833

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS51483 B5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFDVAKSDLERLVGREFSVEEWEDLVLYAKCELDDVWEEDGKIYFKLDSKDTNRPDLW
CCCCCCCHHHHHHHHCCCCCHHHHCCEEEEEECCCHHHHCCCCEEEEEECCCCCCCCCCC
SAEGVARQIRWALGLAKGLPKYEVEESDVVVYVDKKLRNIRPYGVYAVVEGLKLDEEALS
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHCCCCCHHHHH
QLIQLQEKVALTYGRRRREVAIGIFDFDKVKPPIYYRAAEKTEKFVPLGYSEEMTLEEIL
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHH
EKHEKGREYGHLIRDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETKNVFVDVTGWDL
HHHHCCCHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEECCCEEEEEECCHHH
RKVMLALNVIVTALAERGGKIKRVKVIYPDFEITTPDLTPKEFEVSFEYIRKLSGLELSN
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCH
EEIKELLERMMYEVEILSENKAKVKYPAFRDDIMHTRDVLEDVLIAYGYNNIDPEEPKLA
HHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VQGRGDPFKDFEDAIRDLMVGFGLQEVMTFNLTSKEVQFDKMNIPEEEIVEIANPISSRW
EECCCCCCHHHHHHHHHHHHHCCHHHHHEECCCCCCEEECCCCCCHHHHHHHHHHHHHHH
SALRKWLLPSLMEFLSNNTHEEYPQRIFEVGLATLIDESRETKTVSEPKLAVALAGSGYT
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCE
FTNAKEILDSLMRHLGIEYDIEETVHGSFIPGRVGKILVDGKEIGIIGEIHPQVLENWNI
ECCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCEEEECCCEEEEEECCCHHHHHCCCC
QVPVVAFEIFLKPLYR
EEHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKFDVAKSDLERLVGREFSVEEWEDLVLYAKCELDDVWEEDGKIYFKLDSKDTNRPDLW
CCCCCCHHHHHHHHCCCCCHHHHCCEEEEEECCCHHHHCCCCEEEEEECCCCCCCCCCC
SAEGVARQIRWALGLAKGLPKYEVEESDVVVYVDKKLRNIRPYGVYAVVEGLKLDEEALS
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHCCCCCCCHHHHHHCCCCCHHHHH
QLIQLQEKVALTYGRRRREVAIGIFDFDKVKPPIYYRAAEKTEKFVPLGYSEEMTLEEIL
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCHHHHH
EKHEKGREYGHLIRDKPYYPLLVDSEGNVLSMPPIINSELTGRVTTETKNVFVDVTGWDL
HHHHCCCHHHHHHCCCCCCCEEECCCCCEEECCCCCCCCCCCEEEECCCEEEEEECCHHH
RKVMLALNVIVTALAERGGKIKRVKVIYPDFEITTPDLTPKEFEVSFEYIRKLSGLELSN
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCCCCCH
EEIKELLERMMYEVEILSENKAKVKYPAFRDDIMHTRDVLEDVLIAYGYNNIDPEEPKLA
HHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
VQGRGDPFKDFEDAIRDLMVGFGLQEVMTFNLTSKEVQFDKMNIPEEEIVEIANPISSRW
EECCCCCCHHHHHHHHHHHHHCCHHHHHEECCCCCCEEECCCCCCHHHHHHHHHHHHHHH
SALRKWLLPSLMEFLSNNTHEEYPQRIFEVGLATLIDESRETKTVSEPKLAVALAGSGYT
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCE
FTNAKEILDSLMRHLGIEYDIEETVHGSFIPGRVGKILVDGKEIGIIGEIHPQVLENWNI
ECCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCEEEECCCEEEEEECCCHHHHHCCCC
QVPVVAFEIFLKPLYR
EEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA