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The map label for this gene is pheS

Identifier: 18977361

GI number: 18977361

Start: 942767

End: 944266

Strand: Direct

Name: pheS

Synonym: PF0989

Alternate gene names: 18977361

Gene position: 942767-944266 (Clockwise)

Preceding gene: 18977360

Following gene: 18977362

Centisome position: 49.4

GC content: 39.87

Gene sequence:

>1500_bases
ATGTTAGGATACAATGAGAAGCTTGTTCTACTTAAGCTCAACGAAGTAAAAAAAGCAAGAATAGAGGAACTTGTAAAGCT
CACAGGACTTGAGCAAGTTGCGATAATGAGAGCCCTTTTAACCCTTGAAAAAGAGGGTTTAGCCAAGATTTCGGAAAGAA
AAGAGAGAATAATACGATTAACAGATCTAGGCAGAAAATACATTCAGATGGGATTACCTGAAATAAGAGCTTTAAACGTT
TTAAAAAGCAAGAAAAAGGTAAAACTAGATGAATTAAGAGGAGTGCTGACGGAAGATGAGCTAAAGCCAATAGTTGGTAT
TCTAAGAAAGGAAGGATGGGTAAAGGTAGAGAAAACTCCAGAAGGTTTGGTGTTAGAAATAACAGAAAGAGGAGAAAATC
CAGAAGAGAGACCAATAGATAAGGCTCTAAAAATATTATCAGAAAAAGAATATGCCACTTCTGAAGAAATATCAAAAATA
GTTCCCATAAATGAACTCAAGAGAAGAAAAGTTGCCCAGGAGGAGGAAGTAGTTGAGAGGATTGCGGAGATTACTGAAAA
GGGAGTTGAACTAGTGAAAAAAGGAATAGAGTTAAAGAGAGAGGTCACTAGTTTAACTCCAGAACTTATAGCAAGTGGAA
AATGGAGAGAAGTAGAGTTCAAACCTTTCAACATTAAGGCCCCTGTAAAGAAGATATACCCGGGCAAGAAGCAACCATAT
AGAGTATTCTTAGACAAGATAAGGAGAAAATTAATCGAGATGGGATTCATTGAAATAACCGTGGATAGCTTAATTGAAAC
CCAGTTCTGGAACTTTGATGCTCTATTCCAGCCTCAAAACCACCCTGCCAGAGAATGGACAGACACCTACCAGCTGAAAT
ATCCAGAAAAAGGCTATTTACCTAATAGAGAATTAGTGGAAAGAGTAAAGGAGGCCCATGAACGTGGATTGGCTGGCTCA
AGAGGGTGGGGTTACGTATGGAGCCCCGAAAGAGCAATGTTTCTAATGCCCAGGGCCCATGCCACGGCCTTAAGTGCTAG
ACAGCTCGCGAAAGGAATAGAAATTCCTGGGAAGTATTTCACAATACAGAGAGTATTTAGGCCTGATGTCCTAGATAGAA
CACATTTGATAGAATTCAACCAAATTGATGGGTTTGTGGCAGCTGAGGACTTAACCTTTAGGCATCTACTGGGCATACTA
AAGAAGTTTGCAATTGAGATTGCAGGAGCAAAAAAGGTAAAGTTCTTCCCAGATTATTATCCGTTTACAGAACCTAGTGT
TCAGCTAAGTGCATATCACCCTGAATTAGGATGGGTCGAGTTTGGAGGTGCAGGAGTTTTTAGGGAGGAGATGACTAAAG
CTCTAGGAATAGATGTCCCAGTTATAGCATGGGGAATTGGAATAGATAGGCTTGCAATGTTCAGGCTTGGAATAGATGAT
ATTAGATATCTCTTCAGCTACGATCTGAAGTGGCTTAGGGAAGCTAAGCTCATTTGGTAG

Upstream 100 bases:

>100_bases
CACGCTCCCTCGGTCGGGCCGCCCGGGCGGCAGGTCGGACATGTGAATATACCAAAAAGAATTTTTAAATGTATGCCGCT
CACAAAATGGAGGTGTGGAA

Downstream 100 bases:

>100_bases
GTGATGAGAAATGCCAAAGTTTGACGTTGCAAAATCAGACTTGGAAAGATTAGTTGGAAGAGAGTTTTCAGTTGAAGAGT
GGGAAGACCTTGTTCTTTAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLGYNEKLVLLKLNEVKKARIEELVKLTGLEQVAIMRALLTLEKEGLAKISERKERIIRLTDLGRKYIQMGLPEIRALNV
LKSKKKVKLDELRGVLTEDELKPIVGILRKEGWVKVEKTPEGLVLEITERGENPEERPIDKALKILSEKEYATSEEISKI
VPINELKRRKVAQEEEVVERIAEITEKGVELVKKGIELKREVTSLTPELIASGKWREVEFKPFNIKAPVKKIYPGKKQPY
RVFLDKIRRKLIEMGFIEITVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPEKGYLPNRELVERVKEAHERGLAGS
RGWGYVWSPERAMFLMPRAHATALSARQLAKGIEIPGKYFTIQRVFRPDVLDRTHLIEFNQIDGFVAAEDLTFRHLLGIL
KKFAIEIAGAKKVKFFPDYYPFTEPSVQLSAYHPELGWVEFGGAGVFREEMTKALGIDVPVIAWGIGIDRLAMFRLGIDD
IRYLFSYDLKWLREAKLIW

Sequences:

>Translated_499_residues
MLGYNEKLVLLKLNEVKKARIEELVKLTGLEQVAIMRALLTLEKEGLAKISERKERIIRLTDLGRKYIQMGLPEIRALNV
LKSKKKVKLDELRGVLTEDELKPIVGILRKEGWVKVEKTPEGLVLEITERGENPEERPIDKALKILSEKEYATSEEISKI
VPINELKRRKVAQEEEVVERIAEITEKGVELVKKGIELKREVTSLTPELIASGKWREVEFKPFNIKAPVKKIYPGKKQPY
RVFLDKIRRKLIEMGFIEITVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPEKGYLPNRELVERVKEAHERGLAGS
RGWGYVWSPERAMFLMPRAHATALSARQLAKGIEIPGKYFTIQRVFRPDVLDRTHLIEFNQIDGFVAAEDLTFRHLLGIL
KKFAIEIAGAKKVKFFPDYYPFTEPSVQLSAYHPELGWVEFGGAGVFREEMTKALGIDVPVIAWGIGIDRLAMFRLGIDD
IRYLFSYDLKWLREAKLIW
>Mature_499_residues
MLGYNEKLVLLKLNEVKKARIEELVKLTGLEQVAIMRALLTLEKEGLAKISERKERIIRLTDLGRKYIQMGLPEIRALNV
LKSKKKVKLDELRGVLTEDELKPIVGILRKEGWVKVEKTPEGLVLEITERGENPEERPIDKALKILSEKEYATSEEISKI
VPINELKRRKVAQEEEVVERIAEITEKGVELVKKGIELKREVTSLTPELIASGKWREVEFKPFNIKAPVKKIYPGKKQPY
RVFLDKIRRKLIEMGFIEITVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPEKGYLPNRELVERVKEAHERGLAGS
RGWGYVWSPERAMFLMPRAHATALSARQLAKGIEIPGKYFTIQRVFRPDVLDRTHLIEFNQIDGFVAAEDLTFRHLLGIL
KKFAIEIAGAKKVKFFPDYYPFTEPSVQLSAYHPELGWVEFGGAGVFREEMTKALGIDVPVIAWGIGIDRLAMFRLGIDD
IRYLFSYDLKWLREAKLIW

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=338, Percent_Identity=40.8284023668639, Blast_Score=248, Evalue=7e-66,
Organism=Escherichia coli, GI1788007, Length=277, Percent_Identity=27.4368231046931, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17508957, Length=486, Percent_Identity=32.0987654320988, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI32563657, Length=315, Percent_Identity=40, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321087, Length=466, Percent_Identity=30.4721030042918, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI18858079, Length=506, Percent_Identity=31.4229249011858, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PYRFU (Q8U261)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578718.1
- ProteinModelPortal:   Q8U261
- SMR:   Q8U261
- EnsemblBacteria:   EBPYRT00000006035
- GeneID:   1468854
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0989
- NMPDR:   fig|186497.1.peg.1025
- GeneTree:   EBGT00050000023140
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- ProtClustDB:   PRK04172
- BRENDA:   6.1.1.20
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 57751; Mature: 57751

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGYNEKLVLLKLNEVKKARIEELVKLTGLEQVAIMRALLTLEKEGLAKISERKERIIRL
CCCCCCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDLGRKYIQMGLPEIRALNVLKSKKKVKLDELRGVLTEDELKPIVGILRKEGWVKVEKTP
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCC
EGLVLEITERGENPEERPIDKALKILSEKEYATSEEISKIVPINELKRRKVAQEEEVVER
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCHHHHHHHH
IAEITEKGVELVKKGIELKREVTSLTPELIASGKWREVEFKPFNIKAPVKKIYPGKKQPY
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCHH
RVFLDKIRRKLIEMGFIEITVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPEKGYL
HHHHHHHHHHHHHHCHHEEEHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEECCCCCCC
PNRELVERVKEAHERGLAGSRGWGYVWSPERAMFLMPRAHATALSARQLAKGIEIPGKYF
CCHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCEE
TIQRVFRPDVLDRTHLIEFNQIDGFVAAEDLTFRHLLGILKKFAIEIAGAKKVKFFPDYY
EHHHHHCCCCCCCHHEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PFTEPSVQLSAYHPELGWVEFGGAGVFREEMTKALGIDVPVIAWGIGIDRLAMFRLGIDD
CCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCHHEECCCHHHHHHHHHCHHH
IRYLFSYDLKWLREAKLIW
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLGYNEKLVLLKLNEVKKARIEELVKLTGLEQVAIMRALLTLEKEGLAKISERKERIIRL
CCCCCCCEEEEEECHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDLGRKYIQMGLPEIRALNVLKSKKKVKLDELRGVLTEDELKPIVGILRKEGWVKVEKTP
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCCCCEEEECCC
EGLVLEITERGENPEERPIDKALKILSEKEYATSEEISKIVPINELKRRKVAQEEEVVER
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCHHHHHHHH
IAEITEKGVELVKKGIELKREVTSLTPELIASGKWREVEFKPFNIKAPVKKIYPGKKQPY
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCCCHHHHCCCCCCHH
RVFLDKIRRKLIEMGFIEITVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPEKGYL
HHHHHHHHHHHHHHCHHEEEHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEECCCCCCC
PNRELVERVKEAHERGLAGSRGWGYVWSPERAMFLMPRAHATALSARQLAKGIEIPGKYF
CCHHHHHHHHHHHHCCCCCCCCCCEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCEE
TIQRVFRPDVLDRTHLIEFNQIDGFVAAEDLTFRHLLGILKKFAIEIAGAKKVKFFPDYY
EHHHHHCCCCCCCHHEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PFTEPSVQLSAYHPELGWVEFGGAGVFREEMTKALGIDVPVIAWGIGIDRLAMFRLGIDD
CCCCCCEEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCHHEECCCHHHHHHHHHCHHH
IRYLFSYDLKWLREAKLIW
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA