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The map label for this gene is ilvD

Identifier: 18977314

GI number: 18977314

Start: 906942

End: 908597

Strand: Direct

Name: ilvD

Synonym: PF0942

Alternate gene names: 18977314

Gene position: 906942-908597 (Clockwise)

Preceding gene: 18977313

Following gene: 18977315

Centisome position: 47.53

GC content: 45.11

Gene sequence:

>1656_bases
ATGAGAAGTGATGTAATCAAGAAGGGAATTGAAAGAGCTCCCCATCGTTCTCTATTTAAGGCCATGGGCTTCACAGATGA
GGAACTAGAAAGACCATTAATTGGAGTTGCCAATTCATTTAACGAGCTGATCCCTGGGCATATCCATCTAAGAAAGATTG
CTGAGGCGGTAAAAGCTGGAATAAGGATGGCAGGTGGCACTCCTCTAGAGTTTAACACAATAGGAATATGCGATGGAATA
GCAATGAACCACCTGGGAATGAAATATTCACTACCCTCAAGAGAGCTAATAGCGGACAGCGTCGAACTAGTTGCTAGAGT
CTACCACTTTGATGGCATAGTGGTCATAGCATCTTGTGACAAAATAATTCCAGGAATGCTAATGGCCATAGCAAGGTTGA
ACATTCCAGCCATATTCGTCTCCGGTGGTCCAATGCTTCCCGGCAGATTTAAGGGAGAATATGTGGATGTAAAAACTGTC
TTCGAGGCAGTGGGTGCTGTAAAGGCTGGAAAAATGAGTTATGAGGAGCTAAAGCTTCTAGAGAACTTTGCATGCCCAGG
GTGTGGAAGCTGTGCCGGAATGTTTACAGCCAATACCATGAATGCCCTCACAGAGGCTTTGGGAATTTCTCTGCCCTGGA
ACGGAACAGCTCCTGCTCCCTATGCCCACAGGATAAGGATAGCTAAGGAAACTGGAATGCAGATAGTTAAGTTAGTGGAG
AAAGATGTCAAAGCTAGAGACATCCTTACTAGGGAAGCCTTCGAAGATGCAATAGCTGTGGATATGGCCCTGGGAGGATC
AACAAACACGGTCCTCCACCTAATGGCCATTGCAAATGAAGCTAAGGTTGATTTATCTTTGGAAGACTTTGACAGGATTA
GCGAGAAGACCCCAACGTTGGCAAAGCTGAGTCCCGCAGGAAAGCACTTTGTAGTTGATCTTTACGAGGCAGGAGGAATA
CTCGGAGTTATGAAGAGGTTGAGTGAACTGGGGCTTATTCACGAAGACAGGTTAACAGTATCGTTAAAAACAGTGGGAGA
ATTGTTGAAGAGCGCTTTTGTCTCAAGAGATGATGTAATAAGGCCGGTGACAAATCCCTATCATCCAAGGGGAGGAATAA
TGATTCTTAGAGGAACTTTAGCTCCCAACGGTGCTGTGATAAAGGTTTCCGCCGTGGAAGGAGTTGAAGTGTTTGAAGGG
CCAGCAAAGGTCTTTGATAGCGAAGAGGAAGCAACAGCAGCAATTCTATCCGGAAAGATTGAGAGGGGAGATGTTGTTGT
TATAAGATACGAAGGGCCCAAAGGAGGTCCAGGAATGAGAGAAATGCTGACTCCAACTTCTGCAATAGCGGGAATGGGGC
TTGACAAGGATGTTGCATTGGTGACGGATGGGAGATTTTCCGGAGCAACAAGGGGACTTTCAGTTGGTCATGTCTCTCCT
GAAGCTGCTGAAGGAGGGCCAATAGCACTAGTAAAGGATGGAGACATTATCAGAATTGATTTGAAAAATAAGAGGATTGA
TTTACTTGTCGATGAGGAGGAGCTAAGAAAAAGAAGAGAAGAGTGGAAGCCAAAGGTCAAAGAGCTTACAGGATATCTTA
AGAGATACTCAACTCTTGTCACCTCTGCCAACACTGGTGCTATCTTGAGACATTAA

Upstream 100 bases:

>100_bases
CATGGGGCACTGTGGGAGTGTCAACAAACATCATCGAGGCGAGCTTAAACGCAATAATTGACAGTATGGAGTACTGGCTC
ATGAAGGGGAGGGAGAACAA

Downstream 100 bases:

>100_bases
CTTCTCTCTTCTTTCTGTTTTATATCGAAAGAGTTAAGACTCTGTTGGATAGAATGAAAATGGTGGGATAATGCACTATG
AAGAAGTCGAAATCCTCTTT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MRSDVIKKGIERAPHRSLFKAMGFTDEELERPLIGVANSFNELIPGHIHLRKIAEAVKAGIRMAGGTPLEFNTIGICDGI
AMNHLGMKYSLPSRELIADSVELVARVYHFDGIVVIASCDKIIPGMLMAIARLNIPAIFVSGGPMLPGRFKGEYVDVKTV
FEAVGAVKAGKMSYEELKLLENFACPGCGSCAGMFTANTMNALTEALGISLPWNGTAPAPYAHRIRIAKETGMQIVKLVE
KDVKARDILTREAFEDAIAVDMALGGSTNTVLHLMAIANEAKVDLSLEDFDRISEKTPTLAKLSPAGKHFVVDLYEAGGI
LGVMKRLSELGLIHEDRLTVSLKTVGELLKSAFVSRDDVIRPVTNPYHPRGGIMILRGTLAPNGAVIKVSAVEGVEVFEG
PAKVFDSEEEATAAILSGKIERGDVVVIRYEGPKGGPGMREMLTPTSAIAGMGLDKDVALVTDGRFSGATRGLSVGHVSP
EAAEGGPIALVKDGDIIRIDLKNKRIDLLVDEEELRKRREEWKPKVKELTGYLKRYSTLVTSANTGAILRH

Sequences:

>Translated_551_residues
MRSDVIKKGIERAPHRSLFKAMGFTDEELERPLIGVANSFNELIPGHIHLRKIAEAVKAGIRMAGGTPLEFNTIGICDGI
AMNHLGMKYSLPSRELIADSVELVARVYHFDGIVVIASCDKIIPGMLMAIARLNIPAIFVSGGPMLPGRFKGEYVDVKTV
FEAVGAVKAGKMSYEELKLLENFACPGCGSCAGMFTANTMNALTEALGISLPWNGTAPAPYAHRIRIAKETGMQIVKLVE
KDVKARDILTREAFEDAIAVDMALGGSTNTVLHLMAIANEAKVDLSLEDFDRISEKTPTLAKLSPAGKHFVVDLYEAGGI
LGVMKRLSELGLIHEDRLTVSLKTVGELLKSAFVSRDDVIRPVTNPYHPRGGIMILRGTLAPNGAVIKVSAVEGVEVFEG
PAKVFDSEEEATAAILSGKIERGDVVVIRYEGPKGGPGMREMLTPTSAIAGMGLDKDVALVTDGRFSGATRGLSVGHVSP
EAAEGGPIALVKDGDIIRIDLKNKRIDLLVDEEELRKRREEWKPKVKELTGYLKRYSTLVTSANTGAILRH
>Mature_551_residues
MRSDVIKKGIERAPHRSLFKAMGFTDEELERPLIGVANSFNELIPGHIHLRKIAEAVKAGIRMAGGTPLEFNTIGICDGI
AMNHLGMKYSLPSRELIADSVELVARVYHFDGIVVIASCDKIIPGMLMAIARLNIPAIFVSGGPMLPGRFKGEYVDVKTV
FEAVGAVKAGKMSYEELKLLENFACPGCGSCAGMFTANTMNALTEALGISLPWNGTAPAPYAHRIRIAKETGMQIVKLVE
KDVKARDILTREAFEDAIAVDMALGGSTNTVLHLMAIANEAKVDLSLEDFDRISEKTPTLAKLSPAGKHFVVDLYEAGGI
LGVMKRLSELGLIHEDRLTVSLKTVGELLKSAFVSRDDVIRPVTNPYHPRGGIMILRGTLAPNGAVIKVSAVEGVEVFEG
PAKVFDSEEEATAAILSGKIERGDVVVIRYEGPKGGPGMREMLTPTSAIAGMGLDKDVALVTDGRFSGATRGLSVGHVSP
EAAEGGPIALVKDGDIIRIDLKNKRIDLLVDEEELRKRREEWKPKVKELTGYLKRYSTLVTSANTGAILRH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=45.049504950495, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1786464, Length=496, Percent_Identity=34.4758064516129, Blast_Score=225, Evalue=7e-60,
Organism=Escherichia coli, GI1788157, Length=533, Percent_Identity=32.6454033771107, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI2367371, Length=570, Percent_Identity=31.7543859649123, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6322476, Length=545, Percent_Identity=43.1192660550459, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_PYRFU (Q8U297)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578671.1
- ProteinModelPortal:   Q8U297
- SMR:   Q8U297
- EnsemblBacteria:   EBPYRT00000004363
- GeneID:   1468806
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0942
- NMPDR:   fig|186497.1.peg.977
- GeneTree:   EBGT00050000022695
- HOGENOM:   HBG671001
- OMA:   AKETPQL
- ProtClustDB:   PRK00911
- BRENDA:   4.2.1.9
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59442; Mature: 59442

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDVIKKGIERAPHRSLFKAMGFTDEELERPLIGVANSFNELIPGHIHLRKIAEAVKAG
CCHHHHHHHHHHCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IRMAGGTPLEFNTIGICDGIAMNHLGMKYSLPSRELIADSVELVARVYHFDGIVVIASCD
HEECCCCCEEECEEEEECCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
KIIPGMLMAIARLNIPAIFVSGGPMLPGRFKGEYVDVKTVFEAVGAVKAGKMSYEELKLL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHCCHHCCCCCHHHHHHH
ENFACPGCGSCAGMFTANTMNALTEALGISLPWNGTAPAPYAHRIRIAKETGMQIVKLVE
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHH
KDVKARDILTREAFEDAIAVDMALGGSTNTVLHLMAIANEAKVDLSLEDFDRISEKTPTL
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHEEEEECCCCCEEECCHHHHHHHHHCCCCC
AKLSPAGKHFVVDLYEAGGILGVMKRLSELGLIHEDRLTVSLKTVGELLKSAFVSRDDVI
EECCCCCCEEEEEEHHCCCHHHHHHHHHHCCCEECCCEEEEHHHHHHHHHHHHCCCCCCC
RPVTNPYHPRGGIMILRGTLAPNGAVIKVSAVEGVEVFEGPAKVFDSEEEATAAILSGKI
CCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHCCHHHHCCCCHHHHHHHHHCCC
ERGDVVVIRYEGPKGGPGMREMLTPTSAIAGMGLDKDVALVTDGRFSGATRGLSVGHVSP
CCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCCEECCCCC
EAAEGGPIALVKDGDIIRIDLKNKRIDLLVDEEELRKRREEWKPKVKELTGYLKRYSTLV
CCCCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TSANTGAILRH
HCCCCCCEECC
>Mature Secondary Structure
MRSDVIKKGIERAPHRSLFKAMGFTDEELERPLIGVANSFNELIPGHIHLRKIAEAVKAG
CCHHHHHHHHHHCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IRMAGGTPLEFNTIGICDGIAMNHLGMKYSLPSRELIADSVELVARVYHFDGIVVIASCD
HEECCCCCEEECEEEEECCHHHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEEECC
KIIPGMLMAIARLNIPAIFVSGGPMLPGRFKGEYVDVKTVFEAVGAVKAGKMSYEELKLL
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEHHHHHHHHHCCHHCCCCCHHHHHHH
ENFACPGCGSCAGMFTANTMNALTEALGISLPWNGTAPAPYAHRIRIAKETGMQIVKLVE
HHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEHHHHHHHHHHHH
KDVKARDILTREAFEDAIAVDMALGGSTNTVLHLMAIANEAKVDLSLEDFDRISEKTPTL
HHHHHHHHHHHHHHHHHHEEEEECCCCCHHHEEEEECCCCCEEECCHHHHHHHHHCCCCC
AKLSPAGKHFVVDLYEAGGILGVMKRLSELGLIHEDRLTVSLKTVGELLKSAFVSRDDVI
EECCCCCCEEEEEEHHCCCHHHHHHHHHHCCCEECCCEEEEHHHHHHHHHHHHCCCCCCC
RPVTNPYHPRGGIMILRGTLAPNGAVIKVSAVEGVEVFEGPAKVFDSEEEATAAILSGKI
CCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCHHHCCHHHHCCCCHHHHHHHHHCCC
ERGDVVVIRYEGPKGGPGMREMLTPTSAIAGMGLDKDVALVTDGRFSGATRGLSVGHVSP
CCCCEEEEEECCCCCCCCHHHHHCCHHHHHCCCCCCCEEEEECCCCCCCCCCCEECCCCC
EAAEGGPIALVKDGDIIRIDLKNKRIDLLVDEEELRKRREEWKPKVKELTGYLKRYSTLV
CCCCCCCEEEEECCCEEEEEECCCEEEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TSANTGAILRH
HCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA