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The map label for this gene is leuB [H]

Identifier: 18977312

GI number: 18977312

Start: 904320

End: 905387

Strand: Direct

Name: leuB [H]

Synonym: PF0940

Alternate gene names: 18977312

Gene position: 904320-905387 (Clockwise)

Preceding gene: 18977311

Following gene: 18977313

Centisome position: 47.39

GC content: 44.1

Gene sequence:

>1068_bases
ATGATAAAGATAGCTGTGATCCCAGGTGATGGGATAGGAAAGGAGGTAGTGGCTGAGGGGCTAAAGGTTCTTCGCAAAAT
AGAGGAGCTGAGCAACGTTAAGTTCGACTTTCAAGAGTATCCATTCGGAGCAGAACATTACTTGAAAACTGGAGAAACCC
TTCCAGACTGGGCTCTTGAGGAGTTTAGACACTTTGATGCAATTTACTTTGGAGCAATTGGGGATCCAAGGGTAAAGCCG
GGAATTCTAGAACACGGAATTCTACTTAAGCTCAGATTTTCCCTAGACCTTTACGTAAACTTAAGACCTGTAAAGTTATA
CCACCCAAAACTAACTCCGCTAAAGGGAAAGGAAAAGATTGACATGGTGTTCATAAGGGAAAACACTGAGGGCCTATATG
CAGGAGCGGGTGGTTTTCTTAGGAAGGGGACCCCTCATGAGGTCGCTATTCAAGAAATGATAAATACTAGGTTTGGAGTC
GAGAGAACCATTAGGTTTGCCTTTGAATATGCAAAGACTAAGGGGAGAAAAAAGGTAACTCTAGTAGATAAGGCCAACGT
TCTTACCTATGCTCACGATCTCTGGCAGAGAGTGTTTAAGGAAGTTGCTTCGGAATACCAGGAAATAGAGACTGACCACT
ACTATGTTGATGCTATGGCAATGAAAATGATCCGTTCTCCAGAGATCTTCGAAGTAGTTGTAACTCCAAACATGTTTGGA
GACATTCTCACTGACTTGGGAGCAGAGATTGTGGGAGGATTAGGATTAGCAGCTTCTGGAAATATCAATCCAAGGGGAGT
TTCCATGTTTGAGCCAGTCCACGGTTCTGCCCCAGACATTGCAGGAAAGGGCATTGCAAATCCACTAGCTGCGATTTTAA
CAGCAGCTCTAATGCTTGAGCACCTGGGATTGGACAGGGAAGCCTCACTTGTGGAAAAGGCCGTTGCGAAGACAATAGAA
GAAAACAAAGTAACACCAGACTTGGGAGGAGAGTTGAAGACTAGCGAAGTTGGTAATGAAGTTGTGAAAAATTTAGAGGT
GTTATGGGATGAGGGAGAGGAGAGTTGA

Upstream 100 bases:

>100_bases
CAAAGGGGGAAGTATATCACGCCAACCCATTTCCCGAATTTATAATGGAAATCATCAAGGCTGGAGGGCTTGTTGAATGG
GCTAAGAGGAGGTTGGCAAA

Downstream 100 bases:

>100_bases
GCTATATGATACAACACTTAGAGATGGCTCCCAAATGGAGGGGATAAGCTTTTCCCTTGAGGATAAGCTAAAGATAACTG
AAAAGCTCGATGAATTTGGA

Product: 3-isopropylmalate dehydrogenase 2

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIKIAVIPGDGIGKEVVAEGLKVLRKIEELSNVKFDFQEYPFGAEHYLKTGETLPDWALEEFRHFDAIYFGAIGDPRVKP
GILEHGILLKLRFSLDLYVNLRPVKLYHPKLTPLKGKEKIDMVFIRENTEGLYAGAGGFLRKGTPHEVAIQEMINTRFGV
ERTIRFAFEYAKTKGRKKVTLVDKANVLTYAHDLWQRVFKEVASEYQEIETDHYYVDAMAMKMIRSPEIFEVVVTPNMFG
DILTDLGAEIVGGLGLAASGNINPRGVSMFEPVHGSAPDIAGKGIANPLAAILTAALMLEHLGLDREASLVEKAVAKTIE
ENKVTPDLGGELKTSEVGNEVVKNLEVLWDEGEES

Sequences:

>Translated_355_residues
MIKIAVIPGDGIGKEVVAEGLKVLRKIEELSNVKFDFQEYPFGAEHYLKTGETLPDWALEEFRHFDAIYFGAIGDPRVKP
GILEHGILLKLRFSLDLYVNLRPVKLYHPKLTPLKGKEKIDMVFIRENTEGLYAGAGGFLRKGTPHEVAIQEMINTRFGV
ERTIRFAFEYAKTKGRKKVTLVDKANVLTYAHDLWQRVFKEVASEYQEIETDHYYVDAMAMKMIRSPEIFEVVVTPNMFG
DILTDLGAEIVGGLGLAASGNINPRGVSMFEPVHGSAPDIAGKGIANPLAAILTAALMLEHLGLDREASLVEKAVAKTIE
ENKVTPDLGGELKTSEVGNEVVKNLEVLWDEGEES
>Mature_355_residues
MIKIAVIPGDGIGKEVVAEGLKVLRKIEELSNVKFDFQEYPFGAEHYLKTGETLPDWALEEFRHFDAIYFGAIGDPRVKP
GILEHGILLKLRFSLDLYVNLRPVKLYHPKLTPLKGKEKIDMVFIRENTEGLYAGAGGFLRKGTPHEVAIQEMINTRFGV
ERTIRFAFEYAKTKGRKKVTLVDKANVLTYAHDLWQRVFKEVASEYQEIETDHYYVDAMAMKMIRSPEIFEVVVTPNMFG
DILTDLGAEIVGGLGLAASGNINPRGVSMFEPVHGSAPDIAGKGIANPLAAILTAALMLEHLGLDREASLVEKAVAKTIE
ENKVTPDLGGELKTSEVGNEVVKNLEVLWDEGEES

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=36.2857142857143, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI4758582, Length=355, Percent_Identity=31.5492957746479, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI28178821, Length=347, Percent_Identity=31.9884726224784, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=31.8840579710145, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28178838, Length=324, Percent_Identity=31.4814814814815, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI28178819, Length=197, Percent_Identity=37.0558375634518, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1788101, Length=353, Percent_Identity=43.0594900849858, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI87081683, Length=353, Percent_Identity=38.5269121813031, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1787381, Length=392, Percent_Identity=30.3571428571429, Blast_Score=141, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71986051, Length=348, Percent_Identity=34.7701149425287, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17550882, Length=353, Percent_Identity=33.9943342776204, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17505779, Length=350, Percent_Identity=32, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI25144293, Length=349, Percent_Identity=31.5186246418338, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6322097, Length=356, Percent_Identity=38.4831460674157, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=36.5650969529086, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=35.3448275862069, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=35.6741573033708, Blast_Score=174, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24643268, Length=347, Percent_Identity=36.8876080691643, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24643270, Length=347, Percent_Identity=36.8876080691643, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24661184, Length=352, Percent_Identity=33.8068181818182, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI161078633, Length=355, Percent_Identity=30.4225352112676, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24650122, Length=355, Percent_Identity=30.4225352112676, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078639, Length=353, Percent_Identity=30.5949008498584, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI161078637, Length=345, Percent_Identity=30.4347826086957, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI161078635, Length=345, Percent_Identity=30.4347826086957, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=31.2138728323699, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=31.2138728323699, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI20130355, Length=227, Percent_Identity=28.6343612334802, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39412; Mature: 39412

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIAVIPGDGIGKEVVAEGLKVLRKIEELSNVKFDFQEYPFGAEHYLKTGETLPDWALE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHCCCCCCHHHHH
EFRHFDAIYFGAIGDPRVKPGILEHGILLKLRFSLDLYVNLRPVKLYHPKLTPLKGKEKI
HHHHHCEEEECCCCCCCCCCCHHCCCEEEEEEEEEEEEEEEEEEEEECCCCCCCCCCCCE
DMVFIRENTEGLYAGAGGFLRKGTPHEVAIQEMINTRFGVERTIRFAFEYAKTKGRKKVT
EEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEE
LVDKANVLTYAHDLWQRVFKEVASEYQEIETDHYYVDAMAMKMIRSPEIFEVVVTPNMFG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHH
DILTDLGAEIVGGLGLAASGNINPRGVSMFEPVHGSAPDIAGKGIANPLAAILTAALMLE
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HLGLDREASLVEKAVAKTIEENKVTPDLGGELKTSEVGNEVVKNLEVLWDEGEES
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIKIAVIPGDGIGKEVVAEGLKVLRKIEELSNVKFDFQEYPFGAEHYLKTGETLPDWALE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHCCCCCCHHHHH
EFRHFDAIYFGAIGDPRVKPGILEHGILLKLRFSLDLYVNLRPVKLYHPKLTPLKGKEKI
HHHHHCEEEECCCCCCCCCCCHHCCCEEEEEEEEEEEEEEEEEEEEECCCCCCCCCCCCE
DMVFIRENTEGLYAGAGGFLRKGTPHEVAIQEMINTRFGVERTIRFAFEYAKTKGRKKVT
EEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEE
LVDKANVLTYAHDLWQRVFKEVASEYQEIETDHYYVDAMAMKMIRSPEIFEVVVTPNMFG
EEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCHHHH
DILTDLGAEIVGGLGLAASGNINPRGVSMFEPVHGSAPDIAGKGIANPLAAILTAALMLE
HHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HLGLDREASLVEKAVAKTIEENKVTPDLGGELKTSEVGNEVVKNLEVLWDEGEES
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA