Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 18977307

GI number: 18977307

Start: 898325

End: 900019

Strand: Direct

Name: ilvB [H]

Synonym: PF0935

Alternate gene names: 18977307

Gene position: 898325-900019 (Clockwise)

Preceding gene: 18977306

Following gene: 18977308

Centisome position: 47.08

GC content: 45.66

Gene sequence:

>1695_bases
ATGGAAATGTCCGGTGCAAAAGCCCTCGTCCGGGCCCTCGAAAAAGAAGGCGTGCAACACACGTTTGGGATAATTGGTGG
AAGTATAATGCCAGTGTTTGATGAACTACTAGATAGCAACATAAGACACATAACCACTAGACACGAGCAGGGAGCAGCCC
ATGCTGCAGATGGATACGCAAGAGTAAGTGGAAGGCCTGGGGTGGCAATAACTACCTCAGGCCCAGGGGCCACAAATCTA
GTTACTGGAATAGCTACGGCATATATGGACTCATCACCTATAGTAGCATTGACAGGGCAGGTTCCAACATACATGGTAGG
AAAGATGGCGTTTCAAGAAACGGATATCATAAACATAACTAAGCCAATAACAAAGTGGAATTACCAGGTGAAAAGACCGA
GGGAGATCCCCGAAGCTGTAAAAATGGCATTCACAATCGCCACACTAGGAAGACCTGGCCCTACACTAATTGACCTTCCT
AAGGATGCTCAAACAGATGTTGAAGATGTAGATTTTGACGTTGATATTTCCCAAAAGGTAACGCCCTGGAGACCACCTAA
GCTTGATGCTTGCCCTGAAGAGGTAAAGAAAGCTGTTGACATGATTCTCTCAGCTGAGAGACCTGTGATCATTGTTGGTG
GAGGAGTTGTTTGGTCAGGAGCAACTGATGAAGTTTTGGCTATAGCTGAGTACTTGCTAATCCCAATAATGGCCACCTTC
ATGGGGAAAGGAGCCGTCCCAGAAAATCATCCACTCTACGTGGGAAATCTTGGAATGCACGGAAAAATTGCTGCAAACAA
ACTCCTTCCTCAGACTGATTTGATAATAGCTGTAGGAATGAGGTGGAGCGATAGGACAGTTAGCGAATTTGAGAACTTTG
CTCCAGAGGCTAAGATAATACACATTGACATTGACCCGAAGGAAGTTGGGAAGAACGTAAAAGTGGACCTAGGGATTATA
GGGGATGCCAAGAGAGTACTCAGGGCTATCTACAACGAAATTGTTAGAAGGGCAAAGAAAAGAGAGAGCTGGCCATGGTT
AGAGAAAGTTAGGGAATTCAAAGAGAAATATAAAGAAGAGCTAGTCCCAATTGATGGGGACTATCTCAGACCCCCTGAGA
TTTTGAAGGAGTTGAGGAAGCTCCTCCCTCCAGATGCCATAGTTGCAACTGAAGTTGGACAAAATCAAATGTGGGTAGCG
TTGTTCTTCCCAATACTAAAGCCTAGGACTTTCTTGACTTCGGGTGGATTGGGGACAATGGGCTTTGGCTTCCCAGCGGC
CATAGGGGCAAAGGTAGCAAAGCCAGAGAAGGTAGTTGTGGATATTGCGGGTGATGGGAGCTTTATGATGTCCGAAAGAG
AATTGGCCACAGCAGTCAACGAAAATCTTCCAGTAATAGTGATAATTCTCAACAACTCCTCCCTGGGAATGGTCGCCCAA
TGGCAGAGAATGTTCTACAATAGAAGATACATAGCTACTTATTTCAAAAAGAATCCTGATTTTGTCAAGCTGGCTGAGGC
TTATGGAGCCAATGGATACAAGGCTGAGACTATGGATGAGTTGCTCAAAGCGGTGAAGGAAGGAATGAACTCTGATGTTC
CCACGGTTATAGACGTCCCAATCCATCCGGAGGATGATGTTCTCCCAATGGTTCCTCCTGGGCAACACATCTCAAACGTT
GTGACAAGGTATTGA

Upstream 100 bases:

>100_bases
TTATTCTAGCTATTATTGGGCTTATTATTCTTCTCTGAAGGGAAAGGGGCAACTTCTGGCCCCTTCTAAGCTTGTATAAA
AATTTTGGGGGTGCAACTCC

Downstream 100 bases:

>100_bases
GGTGGTTAGAGTGGTAAAGGTTTGGTATGACAACGAGGTAAGTATGGATATACTCAAGGACAAGACCGTGGCTGTTATAG
GGTATGGAAGCCAGGGTGAA

Product: acetolactate synthase

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MEMSGAKALVRALEKEGVQHTFGIIGGSIMPVFDELLDSNIRHITTRHEQGAAHAADGYARVSGRPGVAITTSGPGATNL
VTGIATAYMDSSPIVALTGQVPTYMVGKMAFQETDIINITKPITKWNYQVKRPREIPEAVKMAFTIATLGRPGPTLIDLP
KDAQTDVEDVDFDVDISQKVTPWRPPKLDACPEEVKKAVDMILSAERPVIIVGGGVVWSGATDEVLAIAEYLLIPIMATF
MGKGAVPENHPLYVGNLGMHGKIAANKLLPQTDLIIAVGMRWSDRTVSEFENFAPEAKIIHIDIDPKEVGKNVKVDLGII
GDAKRVLRAIYNEIVRRAKKRESWPWLEKVREFKEKYKEELVPIDGDYLRPPEILKELRKLLPPDAIVATEVGQNQMWVA
LFFPILKPRTFLTSGGLGTMGFGFPAAIGAKVAKPEKVVVDIAGDGSFMMSERELATAVNENLPVIVIILNNSSLGMVAQ
WQRMFYNRRYIATYFKKNPDFVKLAEAYGANGYKAETMDELLKAVKEGMNSDVPTVIDVPIHPEDDVLPMVPPGQHISNV
VTRY

Sequences:

>Translated_564_residues
MEMSGAKALVRALEKEGVQHTFGIIGGSIMPVFDELLDSNIRHITTRHEQGAAHAADGYARVSGRPGVAITTSGPGATNL
VTGIATAYMDSSPIVALTGQVPTYMVGKMAFQETDIINITKPITKWNYQVKRPREIPEAVKMAFTIATLGRPGPTLIDLP
KDAQTDVEDVDFDVDISQKVTPWRPPKLDACPEEVKKAVDMILSAERPVIIVGGGVVWSGATDEVLAIAEYLLIPIMATF
MGKGAVPENHPLYVGNLGMHGKIAANKLLPQTDLIIAVGMRWSDRTVSEFENFAPEAKIIHIDIDPKEVGKNVKVDLGII
GDAKRVLRAIYNEIVRRAKKRESWPWLEKVREFKEKYKEELVPIDGDYLRPPEILKELRKLLPPDAIVATEVGQNQMWVA
LFFPILKPRTFLTSGGLGTMGFGFPAAIGAKVAKPEKVVVDIAGDGSFMMSERELATAVNENLPVIVIILNNSSLGMVAQ
WQRMFYNRRYIATYFKKNPDFVKLAEAYGANGYKAETMDELLKAVKEGMNSDVPTVIDVPIHPEDDVLPMVPPGQHISNV
VTRY
>Mature_564_residues
MEMSGAKALVRALEKEGVQHTFGIIGGSIMPVFDELLDSNIRHITTRHEQGAAHAADGYARVSGRPGVAITTSGPGATNL
VTGIATAYMDSSPIVALTGQVPTYMVGKMAFQETDIINITKPITKWNYQVKRPREIPEAVKMAFTIATLGRPGPTLIDLP
KDAQTDVEDVDFDVDISQKVTPWRPPKLDACPEEVKKAVDMILSAERPVIIVGGGVVWSGATDEVLAIAEYLLIPIMATF
MGKGAVPENHPLYVGNLGMHGKIAANKLLPQTDLIIAVGMRWSDRTVSEFENFAPEAKIIHIDIDPKEVGKNVKVDLGII
GDAKRVLRAIYNEIVRRAKKRESWPWLEKVREFKEKYKEELVPIDGDYLRPPEILKELRKLLPPDAIVATEVGQNQMWVA
LFFPILKPRTFLTSGGLGTMGFGFPAAIGAKVAKPEKVVVDIAGDGSFMMSERELATAVNENLPVIVIILNNSSLGMVAQ
WQRMFYNRRYIATYFKKNPDFVKLAEAYGANGYKAETMDELLKAVKEGMNSDVPTVIDVPIHPEDDVLPMVPPGQHISNV
VTRY

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=26.8551236749117, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI21361361, Length=595, Percent_Identity=26.3865546218487, Blast_Score=185, Evalue=9e-47,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=40.5357142857143, Blast_Score=440, Evalue=1e-124,
Organism=Escherichia coli, GI1790104, Length=555, Percent_Identity=41.6216216216216, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=32.7956989247312, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=27.2232304900181, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=27.2890484739677, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17531301, Length=571, Percent_Identity=28.0210157618214, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17531299, Length=571, Percent_Identity=28.0210157618214, Blast_Score=188, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17542570, Length=566, Percent_Identity=27.208480565371, Blast_Score=172, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=38.5542168674699, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320816, Length=570, Percent_Identity=25.6140350877193, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323163, Length=499, Percent_Identity=24.0480961923848, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=537, Percent_Identity=24.0223463687151, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=494, Percent_Identity=23.4817813765182, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=25.9649122807018, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62048; Mature: 62048

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMSGAKALVRALEKEGVQHTFGIIGGSIMPVFDELLDSNIRHITTRHEQGAAHAADGYA
CCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEE
RVSGRPGVAITTSGPGATNLVTGIATAYMDSSPIVALTGQVPTYMVGKMAFQETDIINIT
EECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEC
KPITKWNYQVKRPREIPEAVKMAFTIATLGRPGPTLIDLPKDAQTDVEDVDFDVDISQKV
CCHHHCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCCCCCCCCC
TPWRPPKLDACPEEVKKAVDMILSAERPVIIVGGGVVWSGATDEVLAIAEYLLIPIMATF
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHH
MGKGAVPENHPLYVGNLGMHGKIAANKLLPQTDLIIAVGMRWSDRTVSEFENFAPEAKII
HCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEE
HIDIDPKEVGKNVKVDLGIIGDAKRVLRAIYNEIVRRAKKRESWPWLEKVREFKEKYKEE
EEECCHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LVPIDGDYLRPPEILKELRKLLPPDAIVATEVGQNQMWVALFFPILKPRTFLTSGGLGTM
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHHHHCCCCEEECCCCCCC
GFGFPAAIGAKVAKPEKVVVDIAGDGSFMMSERELATAVNENLPVIVIILNNSSLGMVAQ
CCCCHHHHCCCCCCCCEEEEEEECCCCEEECHHHHHHHHCCCCCEEEEEECCCCCCHHHH
WQRMFYNRRYIATYFKKNPDFVKLAEAYGANGYKAETMDELLKAVKEGMNSDVPTVIDVP
HHHHHHCCHHEEHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
IHPEDDVLPMVPPGQHISNVVTRY
CCCCCCEECCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEMSGAKALVRALEKEGVQHTFGIIGGSIMPVFDELLDSNIRHITTRHEQGAAHAADGYA
CCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEE
RVSGRPGVAITTSGPGATNLVTGIATAYMDSSPIVALTGQVPTYMVGKMAFQETDIINIT
EECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEC
KPITKWNYQVKRPREIPEAVKMAFTIATLGRPGPTLIDLPKDAQTDVEDVDFDVDISQKV
CCHHHCCCEECCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCCCCCCCCC
TPWRPPKLDACPEEVKKAVDMILSAERPVIIVGGGVVWSGATDEVLAIAEYLLIPIMATF
CCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHH
MGKGAVPENHPLYVGNLGMHGKIAANKLLPQTDLIIAVGMRWSDRTVSEFENFAPEAKII
HCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEE
HIDIDPKEVGKNVKVDLGIIGDAKRVLRAIYNEIVRRAKKRESWPWLEKVREFKEKYKEE
EEECCHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LVPIDGDYLRPPEILKELRKLLPPDAIVATEVGQNQMWVALFFPILKPRTFLTSGGLGTM
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHHHHCCCCEEECCCCCCC
GFGFPAAIGAKVAKPEKVVVDIAGDGSFMMSERELATAVNENLPVIVIILNNSSLGMVAQ
CCCCHHHHCCCCCCCCEEEEEEECCCCEEECHHHHHHHHCCCCCEEEEEECCCCCCHHHH
WQRMFYNRRYIATYFKKNPDFVKLAEAYGANGYKAETMDELLKAVKEGMNSDVPTVIDVP
HHHHHHCCHHEEHHHHCCCCCEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
IHPEDDVLPMVPPGQHISNVVTRY
CCCCCCEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]