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The map label for this gene is truA

Identifier: 18977290

GI number: 18977290

Start: 886345

End: 887145

Strand: Direct

Name: truA

Synonym: PF0918

Alternate gene names: 18977290

Gene position: 886345-887145 (Clockwise)

Preceding gene: 33359526

Following gene: 18977291

Centisome position: 46.45

GC content: 41.07

Gene sequence:

>801_bases
ATGAGGGTTGCCCTTAAAATAGCCTACGATGGAACTAAGTTTCATGGCTTTCAGAGGCAGCCAAATGTAAGGACCATAGA
GGGGGAAATTTTAAGGGCTTTGAAGGATGCTGGCATTGAATTTGAAAATTTCAAAAGTGCATCAAGGACTGACAGGGGAG
TTAGTGCAAGAGGAAACGTTATTGCCTTATCAACGGAAGATGACAGGATAAAGAATCCTATGGTTTTAAATTCTAGAATG
TCTGATGTGTGGATCTGGGGAATAGCTGAGGTTCCTGAGGACTTCCATCCCAGATTTTGGGCCAATACCAAAGTTTATAG
ATACTATCTCCCCTCCCTGGGCATGAATATTAAGAAGATGAAAGAATGCTCCCTTCTCTTCCTGGGAACTCATGACTTCT
CCGCATTCTCAAGGGTGGATGGGAGAGACACAATTAGGAGCATAGATAGGATTGAAATCTGGGAGAAATGCAATGTTGTT
GTAGTGGAGATTGAGGGTAAGAGCTTTCTGTGGGAGATGGTTAGGAGAATAGTTTCAGCCCTAGTTCTTTGTTCCCAGGG
GGTATTGGCAGAAGAAAGAATTGTAGAAATGCTCAATGGAAAGTTTGAAAAAAGCAGAAAGGTTCCGCCTGCACCTCCCG
AAGGGTTATTGCTTTGGGATATTAAGTATGAGAATGTCGAGTTCCAAATTGACAATGCGTCACTGAAAAAGTTCCAGAGA
GAAATTGTAGAGAGATTTAAGCTACACGCTTCTCTTTCCGCACTTTATGAAGATTTAATATTAAATGAACAAAAAATATA
A

Upstream 100 bases:

>100_bases
TAAACTCTTCCTCATTATCAAACCTCACTATCCTACTCACGCTACCCCTCATCTCTCCTCATCCCGTGAAGTTATAAAGG
GAGCACACTCATTTTTAAAT

Downstream 100 bases:

>100_bases
ACTCCGTAGTCCAGGAAGATGCGGTGGGTAGTGGATGAGAAAAGTAATCTCAAACGGACATTCATATGCGATCCCAAAAA
GGCTTTCCAAGTCGAAAAGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRVALKIAYDGTKFHGFQRQPNVRTIEGEILRALKDAGIEFENFKSASRTDRGVSARGNVIALSTEDDRIKNPMVLNSRM
SDVWIWGIAEVPEDFHPRFWANTKVYRYYLPSLGMNIKKMKECSLLFLGTHDFSAFSRVDGRDTIRSIDRIEIWEKCNVV
VVEIEGKSFLWEMVRRIVSALVLCSQGVLAEERIVEMLNGKFEKSRKVPPAPPEGLLLWDIKYENVEFQIDNASLKKFQR
EIVERFKLHASLSALYEDLILNEQKI

Sequences:

>Translated_266_residues
MRVALKIAYDGTKFHGFQRQPNVRTIEGEILRALKDAGIEFENFKSASRTDRGVSARGNVIALSTEDDRIKNPMVLNSRM
SDVWIWGIAEVPEDFHPRFWANTKVYRYYLPSLGMNIKKMKECSLLFLGTHDFSAFSRVDGRDTIRSIDRIEIWEKCNVV
VVEIEGKSFLWEMVRRIVSALVLCSQGVLAEERIVEMLNGKFEKSRKVPPAPPEGLLLWDIKYENVEFQIDNASLKKFQR
EIVERFKLHASLSALYEDLILNEQKI
>Mature_266_residues
MRVALKIAYDGTKFHGFQRQPNVRTIEGEILRALKDAGIEFENFKSASRTDRGVSARGNVIALSTEDDRIKNPMVLNSRM
SDVWIWGIAEVPEDFHPRFWANTKVYRYYLPSLGMNIKKMKECSLLFLGTHDFSAFSRVDGRDTIRSIDRIEIWEKCNVV
VVEIEGKSFLWEMVRRIVSALVLCSQGVLAEERIVEMLNGKFEKSRKVPPAPPEGLLLWDIKYENVEFQIDNASLKKFQR
EIVERFKLHASLSALYEDLILNEQKI

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI199559785, Length=278, Percent_Identity=29.4964028776978, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI70166634, Length=274, Percent_Identity=25.9124087591241, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI70166599, Length=274, Percent_Identity=25.9124087591241, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI70166645, Length=274, Percent_Identity=25.9124087591241, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=26.2962962962963, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788657, Length=245, Percent_Identity=29.7959183673469, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71985046, Length=308, Percent_Identity=23.7012987012987, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17565126, Length=273, Percent_Identity=24.5421245421245, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321112, Length=297, Percent_Identity=26.2626262626263, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6325044, Length=126, Percent_Identity=32.5396825396825, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281362146, Length=270, Percent_Identity=23.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648455, Length=270, Percent_Identity=23.3333333333333, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PYRFU (Q8U2C1)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_578647.1
- ProteinModelPortal:   Q8U2C1
- SMR:   Q8U2C1
- EnsemblBacteria:   EBPYRT00000005212
- GeneID:   1468782
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0918
- NMPDR:   fig|186497.1.peg.953
- GeneTree:   EBGT00050000022495
- HOGENOM:   HBG621020
- OMA:   KSFLWEM
- ProtClustDB:   PRK00021
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30774; Mature: 30774

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALKIAYDGTKFHGFQRQPNVRTIEGEILRALKDAGIEFENFKSASRTDRGVSARGNV
CEEEEEEEECCCEECCCCCCCCCEEHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCE
IALSTEDDRIKNPMVLNSRMSDVWIWGIAEVPEDFHPRFWANTKVYRYYLPSLGMNIKKM
EEEECCCCCCCCCEEECCCCCEEEEEEHHHCCHHHCCCCCCCCEEEEEECCCCCCCHHHH
KECSLLFLGTHDFSAFSRVDGRDTIRSIDRIEIWEKCNVVVVEIEGKSFLWEMVRRIVSA
CCCEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
LVLCSQGVLAEERIVEMLNGKFEKSRKVPPAPPEGLLLWDIKYENVEFQIDNASLKKFQR
HHHHCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCEEEEECCHHHHHHHH
EIVERFKLHASLSALYEDLILNEQKI
HHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRVALKIAYDGTKFHGFQRQPNVRTIEGEILRALKDAGIEFENFKSASRTDRGVSARGNV
CEEEEEEEECCCEECCCCCCCCCEEHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCE
IALSTEDDRIKNPMVLNSRMSDVWIWGIAEVPEDFHPRFWANTKVYRYYLPSLGMNIKKM
EEEECCCCCCCCCEEECCCCCEEEEEEHHHCCHHHCCCCCCCCEEEEEECCCCCCCHHHH
KECSLLFLGTHDFSAFSRVDGRDTIRSIDRIEIWEKCNVVVVEIEGKSFLWEMVRRIVSA
CCCEEEEEECCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHH
LVLCSQGVLAEERIVEMLNGKFEKSRKVPPAPPEGLLLWDIKYENVEFQIDNASLKKFQR
HHHHCCCCHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCEEEEECCHHHHHHHH
EIVERFKLHASLSALYEDLILNEQKI
HHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA