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The map label for this gene is tfb [H]

Identifier: 18977059

GI number: 18977059

Start: 694475

End: 695326

Strand: Reverse

Name: tfb [H]

Synonym: PF0687

Alternate gene names: 18977059

Gene position: 695326-694475 (Counterclockwise)

Preceding gene: 18977062

Following gene: 18977058

Centisome position: 36.44

GC content: 40.61

Gene sequence:

>852_bases
ATGTCATCTACTGAGCCCGGAGGTGGTTGGTTGATATATCCTGTGAAATGCCCATATTGTAAGTCCCGTGATTTAGTTTA
TGATAGACAACATGGAGAAGTATTTTGCAAAAAATGTGGCTCTATCCTAGCAACAAACCTTGTCGATTCAGAGTTAAGTA
GAAAGACCAAGACTAATGATATCCCAAGATACACTAAAAGAATTGGAGAGTTCACTAGAGAGAAAATTTACAGGCTTAGA
AAGTGGCAGAAAAAGATCTCTTCCGAGAGAAACCTTGTCCTTGCTATGAGTGAGCTTAGAAGACTTAGTGGAATGTTAAA
ACTGCCAAAATATGTTGAAGAAGAAGCAGCATACCTTTACAGAGAAGCCGCTAAAAGAGGATTAACTAGAAGAATACCTA
TCGAAACAACTGTTGCGGCATGCATCTATGCGACTTGTAGGCTTTTTAAGGTTCCCAGGACTCTTAACGAGATTGCCAGC
TATTCCAAGACCGAGAAGAAAGAGATTATGAAAGCCTTTAGAGTGATAGTTAGGAATCTCAACTTAACACCAAAGATGCT
CTTGGCTAGGCCTACGGATTATGTAGATAAGTTTGCAGATGAGCTCGAATTAAGCGAAAGAGTCAGAAGAAGAACCGTTG
ATATTCTAAGGAGAGCTAATGAAGAAGGAATAACTAGTGGAAAAAATCCTCTTTCCCTTGTGGCTGCCGCTCTATACATA
GCTTCTCTCCTTGAAGGTGAGAGAAGAAGCCAGAAGGAAATTGCTAGAGTAACTGGAGTTAGCGAGATGACCGTGAGAAA
CCGATACAAGGAATTGGTCATGAAACTTGGGTTAAAGAGCTTGTGGTGGTGA

Upstream 100 bases:

>100_bases
TTGTAGGGAATACTAGCTATATTATGAAAAGAAATACTAGCATAACCCTCAAAAGGTCAGCACTTCTAATGATAGTTAAT
AGAGATAACGGGCTCAAAGA

Downstream 100 bases:

>100_bases
TAAGAAGTGAGAGTTATTAAGATTCAAACAAAAGGGGATGTTAGGATTTCTTATGCTATTGAAACTATAAGAATAAATGA
GTGGACTAAGGCTATTGCAG

Product: transcription initiation factor IIB chain b

Products: NA

Alternate protein names: TFIIB [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSSTEPGGGWLIYPVKCPYCKSRDLVYDRQHGEVFCKKCGSILATNLVDSELSRKTKTNDIPRYTKRIGEFTREKIYRLR
KWQKKISSERNLVLAMSELRRLSGMLKLPKYVEEEAAYLYREAAKRGLTRRIPIETTVAACIYATCRLFKVPRTLNEIAS
YSKTEKKEIMKAFRVIVRNLNLTPKMLLARPTDYVDKFADELELSERVRRRTVDILRRANEEGITSGKNPLSLVAAALYI
ASLLEGERRSQKEIARVTGVSEMTVRNRYKELVMKLGLKSLWW

Sequences:

>Translated_283_residues
MSSTEPGGGWLIYPVKCPYCKSRDLVYDRQHGEVFCKKCGSILATNLVDSELSRKTKTNDIPRYTKRIGEFTREKIYRLR
KWQKKISSERNLVLAMSELRRLSGMLKLPKYVEEEAAYLYREAAKRGLTRRIPIETTVAACIYATCRLFKVPRTLNEIAS
YSKTEKKEIMKAFRVIVRNLNLTPKMLLARPTDYVDKFADELELSERVRRRTVDILRRANEEGITSGKNPLSLVAAALYI
ASLLEGERRSQKEIARVTGVSEMTVRNRYKELVMKLGLKSLWW
>Mature_282_residues
SSTEPGGGWLIYPVKCPYCKSRDLVYDRQHGEVFCKKCGSILATNLVDSELSRKTKTNDIPRYTKRIGEFTREKIYRLRK
WQKKISSERNLVLAMSELRRLSGMLKLPKYVEEEAAYLYREAAKRGLTRRIPIETTVAACIYATCRLFKVPRTLNEIASY
SKTEKKEIMKAFRVIVRNLNLTPKMLLARPTDYVDKFADELELSERVRRRTVDILRRANEEGITSGKNPLSLVAAALYIA
SLLEGERRSQKEIARVTGVSEMTVRNRYKELVMKLGLKSLWW

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB) [H]

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger [H]

Homologues:

Organism=Homo sapiens, GI4504193, Length=283, Percent_Identity=27.208480565371, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI22035556, Length=271, Percent_Identity=24.7232472324723, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI22035562, Length=264, Percent_Identity=25.7575757575758, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17564892, Length=284, Percent_Identity=30.2816901408451, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17531397, Length=263, Percent_Identity=22.8136882129278, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321685, Length=265, Percent_Identity=24.5283018867925, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6325343, Length=297, Percent_Identity=24.9158249158249, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136758, Length=270, Percent_Identity=28.5185185185185, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI281361961, Length=271, Percent_Identity=25.4612546125461, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24647658, Length=271, Percent_Identity=25.4612546125461, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137 [H]

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon [H]

EC number: NA

Molecular weight: Translated: 32783; Mature: 32652

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS51134 ZF_TFIIB ; PS00782 TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTEPGGGWLIYPVKCPYCKSRDLVYDRQHGEVFCKKCGSILATNLVDSELSRKTKTND
CCCCCCCCCEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IPRYTKRIGEFTREKIYRLRKWQKKISSERNLVLAMSELRRLSGMLKLPKYVEEEAAYLY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAAKRGLTRRIPIETTVAACIYATCRLFKVPRTLNEIASYSKTEKKEIMKAFRVIVRNL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
NLTPKMLLARPTDYVDKFADELELSERVRRRTVDILRRANEEGITSGKNPLSLVAAALYI
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ASLLEGERRSQKEIARVTGVSEMTVRNRYKELVMKLGLKSLWW
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSTEPGGGWLIYPVKCPYCKSRDLVYDRQHGEVFCKKCGSILATNLVDSELSRKTKTND
CCCCCCCCEEEEECCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
IPRYTKRIGEFTREKIYRLRKWQKKISSERNLVLAMSELRRLSGMLKLPKYVEEEAAYLY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAAKRGLTRRIPIETTVAACIYATCRLFKVPRTLNEIASYSKTEKKEIMKAFRVIVRNL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
NLTPKMLLARPTDYVDKFADELELSERVRRRTVDILRRANEEGITSGKNPLSLVAAALYI
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
ASLLEGERRSQKEIARVTGVSEMTVRNRYKELVMKLGLKSLWW
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8564536 [H]