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The map label for this gene is pccB [H]

Identifier: 18977043

GI number: 18977043

Start: 681821

End: 683389

Strand: Direct

Name: pccB [H]

Synonym: PF0671

Alternate gene names: 18977043

Gene position: 681821-683389 (Clockwise)

Preceding gene: 33359497

Following gene: 18977044

Centisome position: 35.73

GC content: 46.72

Gene sequence:

>1569_bases
ATGAGCATGGAAGAGAAGGTCAAAGATCTTTACGAGAGGAAGAAAAAGATAATGCAAATGGGTGGGGAAGAGGCAATAAA
GAAGCAACATGATAAAGGAAAGCTTACAGCTAGAGAAAGAATCGAGCTTCTCCTAGACCCAGGAAGCTTCGTGGAAATAG
GCATGTTCGTTAAGCACAGGGCCACGGAGTTTGGGATGGACAAGAGAGAACTCCCAGCAGATGGAGTAATAACGGGTTAC
GGAACAATTGATGGAAGGCTTGTCTTTGTCTATGCCCAGGACTTCACGGTAATGGGAGGTTCTCTTGGGGAAATGCATGC
AATGAAGATAAAGAGGATAATGGAGCTGGCTTTGGAGGCTGGAGCTCCAGTTATAGGGCTAAACGATTCTGGTGGAGCTA
GAATACAGGAGGGCGTTGATTCACTTAAGGGGTATGGAGAGATATTCAAGATGAACACAATTCTCAGCGGTGTAGTGCCT
CAAATTACGGCAATTATGGGCCCATGTGCAGGGGGTGCCGTCTACAGTCCAGCAATTGGAGACTTCATACTGATGGTCGA
TAATCCATCAACGTTCATGTTCATCACCGGACCCCAGGTAGTTAAGGCCGTTACGGGAGTGGAGGTATCTCCAGTTCAGC
TTGGTGGTGCAATGGTTCATGCCCAGAAGAGCGGACAGGCTCACTTGATAGGGAAGAGCGATGAGGAAGTTATTATGCTT
ATAAAGAAGCTCTTAAGCTACCTCCCCTCCAACAACATGGAGAAGCCTCCAAAGATAAAGCCCAAGGATGAGCCCTTCAG
AAGAACCCCAGAATTGTATGATATAGTTCCAGATGATCCAAACAAGGGTTACGACGTTAGGCAAGTAATTTATGCAATAG
TTGATAGGGACGAAAACGGAAACCCCGACTTCCTGGAGCTACAGCCATACTTTGCTCCAAATGCTGTGATTGGGTTTGGA
AGGATTAATGGACAGCCCGTTGGAATTGTGGCAAACAATCCAATCCACTTAGCTGGAGTTCTTGACATTGACAGTTCTGA
TAAGATAGCGAGATTCGTGAGGTTCTGTGATGCGTTCAACATACCGATAGTCACCTTAGTTGACGTCCCAGGATATCTCC
CAGGGGTTGACCAGGAGAGTAGGGGAATAATTAGGCACGGGGCAAAGGTTCTCTATGCTTACGCTGAGGCGACAGTCCCA
ATGGTCACTGTTATCCTAAGAAAAGCTTATGGAGGAGCTTACCTTGCAATGGGAAGCAAGCACCTGGGAGCAGACTTCGT
CTTTGCATGGCCTACAGCTGAGATAGCTGTTATGGGGCCTGAAGGTGCTGCAAACATAATATTCAGAAAAGAAATAGCTG
CTGCCGAGAACCCAGAGGAGGTAAGACAGCAAAAGATCAAGGAGTACAGGGAGAAGTTTGCAAACCCATACGTAGCTGCG
GCAAGAGGTTACATTGACGACGTAATAGATCCAGCTGAGACAAGAGCAAAGATAGTTATGGCTTTAGAAGCTTTGGAGAA
CAAGAGGGTTAAGCTTCCTCCAAAGAAGCATGGAAACATTCCACTGTGA

Upstream 100 bases:

>100_bases
TTTGCTGATATTTGTTGGAGAAGAAGGTATTTCATCTTCATAAAAATCCTTTTAAGTTAAAACTTTACAAATATCAACCA
GCCTGGTAGGGGGTAGGAGG

Downstream 100 bases:

>100_bases
GGTGCCCAAAATGAGCCAACTCCTTGAGGGCCTCTATATTACAATTTTAGGAATAGTAGTTGTCTTTTCAGTCCTTGCAA
TCCTAGCAATTGTCATGGAC

Product: methylmalonyl-coadecarboxylase, subunit alpha

Products: NA

Alternate protein names: PCCase; Propanoyl-CoA:carbon dioxide ligase [H]

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MSMEEKVKDLYERKKKIMQMGGEEAIKKQHDKGKLTARERIELLLDPGSFVEIGMFVKHRATEFGMDKRELPADGVITGY
GTIDGRLVFVYAQDFTVMGGSLGEMHAMKIKRIMELALEAGAPVIGLNDSGGARIQEGVDSLKGYGEIFKMNTILSGVVP
QITAIMGPCAGGAVYSPAIGDFILMVDNPSTFMFITGPQVVKAVTGVEVSPVQLGGAMVHAQKSGQAHLIGKSDEEVIML
IKKLLSYLPSNNMEKPPKIKPKDEPFRRTPELYDIVPDDPNKGYDVRQVIYAIVDRDENGNPDFLELQPYFAPNAVIGFG
RINGQPVGIVANNPIHLAGVLDIDSSDKIARFVRFCDAFNIPIVTLVDVPGYLPGVDQESRGIIRHGAKVLYAYAEATVP
MVTVILRKAYGGAYLAMGSKHLGADFVFAWPTAEIAVMGPEGAANIIFRKEIAAAENPEEVRQQKIKEYREKFANPYVAA
ARGYIDDVIDPAETRAKIVMALEALENKRVKLPPKKHGNIPL

Sequences:

>Translated_522_residues
MSMEEKVKDLYERKKKIMQMGGEEAIKKQHDKGKLTARERIELLLDPGSFVEIGMFVKHRATEFGMDKRELPADGVITGY
GTIDGRLVFVYAQDFTVMGGSLGEMHAMKIKRIMELALEAGAPVIGLNDSGGARIQEGVDSLKGYGEIFKMNTILSGVVP
QITAIMGPCAGGAVYSPAIGDFILMVDNPSTFMFITGPQVVKAVTGVEVSPVQLGGAMVHAQKSGQAHLIGKSDEEVIML
IKKLLSYLPSNNMEKPPKIKPKDEPFRRTPELYDIVPDDPNKGYDVRQVIYAIVDRDENGNPDFLELQPYFAPNAVIGFG
RINGQPVGIVANNPIHLAGVLDIDSSDKIARFVRFCDAFNIPIVTLVDVPGYLPGVDQESRGIIRHGAKVLYAYAEATVP
MVTVILRKAYGGAYLAMGSKHLGADFVFAWPTAEIAVMGPEGAANIIFRKEIAAAENPEEVRQQKIKEYREKFANPYVAA
ARGYIDDVIDPAETRAKIVMALEALENKRVKLPPKKHGNIPL
>Mature_521_residues
SMEEKVKDLYERKKKIMQMGGEEAIKKQHDKGKLTARERIELLLDPGSFVEIGMFVKHRATEFGMDKRELPADGVITGYG
TIDGRLVFVYAQDFTVMGGSLGEMHAMKIKRIMELALEAGAPVIGLNDSGGARIQEGVDSLKGYGEIFKMNTILSGVVPQ
ITAIMGPCAGGAVYSPAIGDFILMVDNPSTFMFITGPQVVKAVTGVEVSPVQLGGAMVHAQKSGQAHLIGKSDEEVIMLI
KKLLSYLPSNNMEKPPKIKPKDEPFRRTPELYDIVPDDPNKGYDVRQVIYAIVDRDENGNPDFLELQPYFAPNAVIGFGR
INGQPVGIVANNPIHLAGVLDIDSSDKIARFVRFCDAFNIPIVTLVDVPGYLPGVDQESRGIIRHGAKVLYAYAEATVPM
VTVILRKAYGGAYLAMGSKHLGADFVFAWPTAEIAVMGPEGAANIIFRKEIAAAENPEEVRQQKIKEYREKFANPYVAAA
RGYIDDVIDPAETRAKIVMALEALENKRVKLPPKKHGNIPL

Specific function: This Protein Is A Component Of The Acetyl Coenzyme A Carboxylase Complex; First, Biotin Carboxylase Catalyzes The Carboxylation Of The Carrier Protein And Then The Transcarboxylase Transfers The Carboxyl Group To Form Malonyl-CoA. [C]

COG id: COG4799

COG function: function code I; Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI119943100, Length=525, Percent_Identity=54.8571428571429, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI295821216, Length=545, Percent_Identity=52.8440366972477, Blast_Score=551, Evalue=1e-157,
Organism=Homo sapiens, GI11545863, Length=521, Percent_Identity=36.084452975048, Blast_Score=268, Evalue=1e-71,
Organism=Homo sapiens, GI134142062, Length=395, Percent_Identity=23.2911392405063, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788655, Length=205, Percent_Identity=30.7317073170732, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25147362, Length=512, Percent_Identity=55.859375, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI25147359, Length=512, Percent_Identity=55.859375, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17552936, Length=530, Percent_Identity=36.0377358490566, Blast_Score=290, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6323863, Length=350, Percent_Identity=23.1428571428571, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24586065, Length=534, Percent_Identity=34.8314606741573, Blast_Score=269, Evalue=4e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000438
- InterPro:   IPR000022
- InterPro:   IPR011763
- InterPro:   IPR011762 [H]

Pfam domain/function: PF01039 Carboxyl_trans [H]

EC number: =6.4.1.3 [H]

Molecular weight: Translated: 57113; Mature: 56982

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50980 COA_CT_NTER ; PS50989 COA_CT_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMEEKVKDLYERKKKIMQMGGEEAIKKQHDKGKLTARERIELLLDPGSFVEIGMFVKHR
CCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHEEEEEECCCCCEEHHHHHHHH
ATEFGMDKRELPADGVITGYGTIDGRLVFVYAQDFTVMGGSLGEMHAMKIKRIMELALEA
HHHHCCCHHCCCCCCEEEEECCCCCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHC
GAPVIGLNDSGGARIQEGVDSLKGYGEIFKMNTILSGVVPQITAIMGPCAGGAVYSPAIG
CCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
DFILMVDNPSTFMFITGPQVVKAVTGVEVSPVQLGGAMVHAQKSGQAHLIGKSDEEVIML
CEEEEEECCCEEEEEECHHHHHHHHCCEECCEEECCEEEEECCCCCEEEECCCCHHHHHH
IKKLLSYLPSNNMEKPPKIKPKDEPFRRTPELYDIVPDDPNKGYDVRQVIYAIVDRDENG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCC
NPDFLELQPYFAPNAVIGFGRINGQPVGIVANNPIHLAGVLDIDSSDKIARFVRFCDAFN
CCCEEEECCCCCCCCEEEEECCCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCC
IPIVTLVDVPGYLPGVDQESRGIIRHGAKVLYAYAEATVPMVTVILRKAYGGAYLAMGSK
CCEEEEEECCCCCCCCCCCCCCHHHHCHHEEEEHHHHHHHHHHHHHHHHCCCEEEEECCC
HLGADFVFAWPTAEIAVMGPEGAANIIFRKEIAAAENPEEVRQQKIKEYREKFANPYVAA
CCCCCEEEECCCCEEEEECCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
ARGYIDDVIDPAETRAKIVMALEALENKRVKLPPKKHGNIPL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCC
>Mature Secondary Structure 
SMEEKVKDLYERKKKIMQMGGEEAIKKQHDKGKLTARERIELLLDPGSFVEIGMFVKHR
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCHHHEEEEEECCCCCEEHHHHHHHH
ATEFGMDKRELPADGVITGYGTIDGRLVFVYAQDFTVMGGSLGEMHAMKIKRIMELALEA
HHHHCCCHHCCCCCCEEEEECCCCCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHC
GAPVIGLNDSGGARIQEGVDSLKGYGEIFKMNTILSGVVPQITAIMGPCAGGAVYSPAIG
CCCEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCC
DFILMVDNPSTFMFITGPQVVKAVTGVEVSPVQLGGAMVHAQKSGQAHLIGKSDEEVIML
CEEEEEECCCEEEEEECHHHHHHHHCCEECCEEECCEEEEECCCCCEEEECCCCHHHHHH
IKKLLSYLPSNNMEKPPKIKPKDEPFRRTPELYDIVPDDPNKGYDVRQVIYAIVDRDENG
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCCC
NPDFLELQPYFAPNAVIGFGRINGQPVGIVANNPIHLAGVLDIDSSDKIARFVRFCDAFN
CCCEEEECCCCCCCCEEEEECCCCEEEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCC
IPIVTLVDVPGYLPGVDQESRGIIRHGAKVLYAYAEATVPMVTVILRKAYGGAYLAMGSK
CCEEEEEECCCCCCCCCCCCCCHHHHCHHEEEEHHHHHHHHHHHHHHHHCCCEEEEECCC
HLGADFVFAWPTAEIAVMGPEGAANIIFRKEIAAAENPEEVRQQKIKEYREKFANPYVAA
CCCCCEEEECCCCEEEEECCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHH
ARGYIDDVIDPAETRAKIVMALEALENKRVKLPPKKHGNIPL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830278 [H]