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The map label for this gene is purB [H]

Identifier: 18977039

GI number: 18977039

Start: 676584

End: 677936

Strand: Reverse

Name: purB [H]

Synonym: PF0667

Alternate gene names: 18977039

Gene position: 677936-676584 (Counterclockwise)

Preceding gene: 18977040

Following gene: 18977038

Centisome position: 35.53

GC content: 42.79

Gene sequence:

>1353_bases
ATGGCGATTCACCCAATTGATTATCGCTATGGTAGCGAGGAGATGAAGAGGATATGGGATGAGGAGAATAAGTTGCAAAA
GCTACTTGACGTGGAGGCCGCACTTGCTAGGGCTCATGCTAAGGTAGGAAATATCCCAGAGGAAAGTGCAAGGGTGATTT
CAGAGAGGGCCAACACCAAGTGGGTAAAGCTTGAGAGGGTTAAAGAGATAGAGGCCGAGATTCATCACGACATAATGGCC
GTGGTTAAGGCCTTAAGCGAAGTTTGCGGTGAGCATGGGAAGTACGTTCACCTGGGAGCGACTTCCAACGACATCATCGA
TACTGCAAACGCCCTTCTAATAAAGGAAAGCCTTGAGATCGTGGAGAGAGACCTAAAGGAACTCAGGTCAATATTGAAAA
AGTTAGCTAAGGAGCATATAGATACAGTTTGCATTGGAAGAACTCATGGACAGCATGCAATCCCAACAACATACGGAATG
AAATTTGCCTTGTGGTTGGACGAGATTCAGAGGCACATCGAGAGGTTGTACCAATTAAAGGAGAGAGTTTTAGTTGGAAA
GATGAGAGGAGCTGTGGGAACTGCAGCCTCATTTGGAGAAAAGGCCTTTGAAATAGAGAGGCTAGTTATGGAAGACTTGG
GATTAAAGCCGGCAAGAATAACAAATCAAATAATCCAGAGAGATGTTTATGCCGAGCTTATGTTCTTCTTGGCATTGGTA
GCTTCAACTTTGGACAAGATGGGATTAGAGATAAGAAACCTTCAGAGAACGGAAATTCTTGAGGTTAGTGAGCCCTTTGG
AGAGAAGCAGGTTGGTTCTTCAACAATGCCACACAAGAGGAATCCAATAAGGACTGAGAAAGTCTGTGGATTGGCCAGGG
TTCTTTACTCAAACGTTATTCCAGCCCTCTTAAACAATCCATTGTGGCATGAGAGAGATTTGACGAACTCTTCAGTTGAG
AGGGTAATCCTCCCAGAGAGCTTTATATTGCTTGACGAAATGTTGAAAACAATGAAAAAGGTTCTTTCGGGATTGGAGTT
CTTCCCCGAGAATATTAAGAGAAACCTATATCTAACGAAGAATTTGATAATGGCTGAACCATTAATGCTCAAGCTTGCGG
AGAAAGGAATGGGAAGGCAGGAGGCACATGAGCTAGTTAGGCAAATATCCATGAAGGCCTTTAAGGAGAACAGAGATCTC
TTGGAAGTGGCAAAGGAGAATGAGGAAGTTAGAAAATATTTAACAGAGAAAGATTTTGAATCTTTGAAGCCAGAAAACTA
CATAGGAAAGGCCAGAGAAATAGTAGAGAATGTTATAGCTTATGTTGAAGAAATGGAGGCTAAAGGTCTTTAA

Upstream 100 bases:

>100_bases
GATTCCTTATGTTTTTAATCTAATCTTGTATCTTTTTAACATTCTTACGTCAAATAATAACATTTATATTTCAAAATTTT
ATCACAAAGGTGGTGATTGG

Downstream 100 bases:

>100_bases
TTGTTTTTGAAGACTTTAGAGTGAGCCTCCATATTTTTACTTTTTATCATTGTTGTTTCTTGTGAAACTTTCTAGTATAA
TTAAATTTACTTGCATTTAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAIHPIDYRYGSEEMKRIWDEENKLQKLLDVEAALARAHAKVGNIPEESARVISERANTKWVKLERVKEIEAEIHHDIMA
VVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEIVERDLKELRSILKKLAKEHIDTVCIGRTHGQHAIPTTYGM
KFALWLDEIQRHIERLYQLKERVLVGKMRGAVGTAASFGEKAFEIERLVMEDLGLKPARITNQIIQRDVYAELMFFLALV
ASTLDKMGLEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRTEKVCGLARVLYSNVIPALLNNPLWHERDLTNSSVE
RVILPESFILLDEMLKTMKKVLSGLEFFPENIKRNLYLTKNLIMAEPLMLKLAEKGMGRQEAHELVRQISMKAFKENRDL
LEVAKENEEVRKYLTEKDFESLKPENYIGKAREIVENVIAYVEEMEAKGL

Sequences:

>Translated_450_residues
MAIHPIDYRYGSEEMKRIWDEENKLQKLLDVEAALARAHAKVGNIPEESARVISERANTKWVKLERVKEIEAEIHHDIMA
VVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEIVERDLKELRSILKKLAKEHIDTVCIGRTHGQHAIPTTYGM
KFALWLDEIQRHIERLYQLKERVLVGKMRGAVGTAASFGEKAFEIERLVMEDLGLKPARITNQIIQRDVYAELMFFLALV
ASTLDKMGLEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRTEKVCGLARVLYSNVIPALLNNPLWHERDLTNSSVE
RVILPESFILLDEMLKTMKKVLSGLEFFPENIKRNLYLTKNLIMAEPLMLKLAEKGMGRQEAHELVRQISMKAFKENRDL
LEVAKENEEVRKYLTEKDFESLKPENYIGKAREIVENVIAYVEEMEAKGL
>Mature_449_residues
AIHPIDYRYGSEEMKRIWDEENKLQKLLDVEAALARAHAKVGNIPEESARVISERANTKWVKLERVKEIEAEIHHDIMAV
VKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEIVERDLKELRSILKKLAKEHIDTVCIGRTHGQHAIPTTYGMK
FALWLDEIQRHIERLYQLKERVLVGKMRGAVGTAASFGEKAFEIERLVMEDLGLKPARITNQIIQRDVYAELMFFLALVA
STLDKMGLEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRTEKVCGLARVLYSNVIPALLNNPLWHERDLTNSSVER
VILPESFILLDEMLKTMKKVLSGLEFFPENIKRNLYLTKNLIMAEPLMLKLAEKGMGRQEAHELVRQISMKAFKENRDLL
EVAKENEEVRKYLTEKDFESLKPENYIGKAREIVENVIAYVEEMEAKGL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=450, Percent_Identity=28.4444444444444, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=29.6103896103896, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI19743875, Length=348, Percent_Identity=26.1494252873563, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1787376, Length=469, Percent_Identity=27.7185501066098, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1790398, Length=383, Percent_Identity=25.3263707571802, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=400, Percent_Identity=27.75, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32564234, Length=290, Percent_Identity=29.6551724137931, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17553882, Length=359, Percent_Identity=28.6908077994429, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32565146, Length=353, Percent_Identity=28.8951841359773, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=452, Percent_Identity=30.7522123893805, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324993, Length=204, Percent_Identity=30.8823529411765, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24647570, Length=400, Percent_Identity=30.25, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24640179, Length=349, Percent_Identity=27.5071633237822, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24640177, Length=349, Percent_Identity=27.5071633237822, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI78710009, Length=349, Percent_Identity=27.7936962750716, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI221473854, Length=333, Percent_Identity=23.4234234234234, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI78706858, Length=333, Percent_Identity=23.4234234234234, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24662684, Length=353, Percent_Identity=26.3456090651558, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51587; Mature: 51456

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIHPIDYRYGSEEMKRIWDEENKLQKLLDVEAALARAHAKVGNIPEESARVISERANTK
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
WVKLERVKEIEAEIHHDIMAVVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
VERDLKELRSILKKLAKEHIDTVCIGRTHGQHAIPTTYGMKFALWLDEIQRHIERLYQLK
HHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVLVGKMRGAVGTAASFGEKAFEIERLVMEDLGLKPARITNQIIQRDVYAELMFFLALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASTLDKMGLEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRTEKVCGLARVLYSNVI
HHHHHHHCCHHHCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
PALLNNPLWHERDLTNSSVERVILPESFILLDEMLKTMKKVLSGLEFFPENIKRNLYLTK
HHHHCCCCCCCCCCCCHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NLIMAEPLMLKLAEKGMGRQEAHELVRQISMKAFKENRDLLEVAKENEEVRKYLTEKDFE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
SLKPENYIGKAREIVENVIAYVEEMEAKGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIHPIDYRYGSEEMKRIWDEENKLQKLLDVEAALARAHAKVGNIPEESARVISERANTK
CCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
WVKLERVKEIEAEIHHDIMAVVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
VERDLKELRSILKKLAKEHIDTVCIGRTHGQHAIPTTYGMKFALWLDEIQRHIERLYQLK
HHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ERVLVGKMRGAVGTAASFGEKAFEIERLVMEDLGLKPARITNQIIQRDVYAELMFFLALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASTLDKMGLEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRTEKVCGLARVLYSNVI
HHHHHHHCCHHHCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
PALLNNPLWHERDLTNSSVERVILPESFILLDEMLKTMKKVLSGLEFFPENIKRNLYLTK
HHHHCCCCCCCCCCCCHHHCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
NLIMAEPLMLKLAEKGMGRQEAHELVRQISMKAFKENRDLLEVAKENEEVRKYLTEKDFE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
SLKPENYIGKAREIVENVIAYVEEMEAKGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]