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The map label for this gene is rsmB [H]

Identifier: 18977038

GI number: 18977038

Start: 674979

End: 676337

Strand: Reverse

Name: rsmB [H]

Synonym: PF0666

Alternate gene names: 18977038

Gene position: 676337-674979 (Counterclockwise)

Preceding gene: 18977039

Following gene: 18977037

Centisome position: 35.44

GC content: 41.43

Gene sequence:

>1359_bases
ATGGAAGCCGAGAAAGAAAAGAAGCTATCAATCCCTCCTAGGGGAATAAGAGCGATAATAGAGGCTGTTAGACTTGGAGA
AGTTATAAAACCTAGCCAATATGCTAAAAGAGAAGCCTTTAAGAAGCATGATATTAAGGAGGCCTGGCTAAATAGAGTTT
TAACTATGATATTTTATGACATTATGAAGAAGCAGGGGTTAATTGATAAGGCTATAAAGGATGTTGTTGGTGTTACTCCC
TTGATCTTGGATCCCTGGTTAAGAGCCGCCCTTCGTGTGGCTTTTGATGTTGTTCTTTTCCATGACCCCAATCAAAACAC
ATTGAAAAACCTAAAATGGAAGGCTTCTGACTTCATTTCATCTAAAACCCATCCTTACGTTGGAATGTATTATTGGGATA
TTTTTGAGAGAATATTGGAATACAAGCCTAATCCTAAAACCGAGCTAGAGAGGCTAGAATGGGAATACTTAGCCCCAGCC
TGGCTCATAGAAAGGGTTAGAAAGTTACTTGGGGATGAAACCAAGGCATTCTTTGAGGCCGTAAATAGAAAGCATGAGTG
GATAAGCATAAGGGTAAACACCCTAAAAACTACCCCAGAGGAGGTAATAAAAGAGTTCGAGGAGGAGGGTGTTGAAGTAG
TGCAGAGCAAAAGAATTCCAGTTGTGTTAAAGATAAAGGGGCCGTATGATTTTGATTCTAGCTATTTGTTTAGGAAAGGG
AAGATAATAGTTCAAGAAGAAGCCTCGGCAGTAGCTTCCGTTATTCTAGACCCAAAGCCTGGTGAAGTGGTCGTTGACTT
GGCAGCGGCCCCAGGAGGAAAGACGACACATATGGCAGAACTTATGAAAAACAGGGGAAAGATATTTGCATTTGATATAG
ATAAGGCTAGGATGAAAAGGTTAAAAGACTTCGTTTCAAGAATGGGCATAAAAATAGTAAAACCCATAGTTAAAGACGCC
AGAAAGGCTCCAGAAATTTTAGGAGAAGAGGTAGCTGACAGAGTTATGCTTGACGCTCCCTGTACCTCTTCCGGGACTAT
AGGAAAGAATCCCGAACTTAGGTGGAGGTTGAGAGAGCAGAAAATAGAAGAAATGGCTCAACTTCAGAGGGAGCTTTTGG
AAAGTGCCGCCAAGCTTGTAAAACCTGGAGGTAGGTTACTGTACACAACTTGTAGCATTTTTATTGAGGAAGATGAGGAC
AATGTTTCCTGGTTCTTGGAGACATACAAAGAGTTTAGGCTTGTAAAGCTTTCCGGCCCCTACGACCCAGGATTCCTTGA
GGGAACTATGAGAGCATGGCCCCACAGACATGAAACAATAGGCTTTTTCTATGCACTATTTGAAAAAGTGACACAATAA

Upstream 100 bases:

>100_bases
ATTTAATTCTACAAAACTTGATAAAGTAAAAAATTTGGAAATTTTACTCAATCTGTTAGTATAGATGAATTTAAAAATAG
TAAAAAGAAGTTAGATAGTG

Downstream 100 bases:

>100_bases
ATCCCTCTTCTTTAAATTTATGCTTAATTTTATTATGATTTCTTGTAATATTGCGTATTTTACATTTAGAAAAGGGTTTA
AAGTTTTACATTTCAGTAAA

Product: nol1-nop2-sun family putative nucleolar protein IV

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MEAEKEKKLSIPPRGIRAIIEAVRLGEVIKPSQYAKREAFKKHDIKEAWLNRVLTMIFYDIMKKQGLIDKAIKDVVGVTP
LILDPWLRAALRVAFDVVLFHDPNQNTLKNLKWKASDFISSKTHPYVGMYYWDIFERILEYKPNPKTELERLEWEYLAPA
WLIERVRKLLGDETKAFFEAVNRKHEWISIRVNTLKTTPEEVIKEFEEEGVEVVQSKRIPVVLKIKGPYDFDSSYLFRKG
KIIVQEEASAVASVILDPKPGEVVVDLAAAPGGKTTHMAELMKNRGKIFAFDIDKARMKRLKDFVSRMGIKIVKPIVKDA
RKAPEILGEEVADRVMLDAPCTSSGTIGKNPELRWRLREQKIEEMAQLQRELLESAAKLVKPGGRLLYTTCSIFIEEDED
NVSWFLETYKEFRLVKLSGPYDPGFLEGTMRAWPHRHETIGFFYALFEKVTQ

Sequences:

>Translated_452_residues
MEAEKEKKLSIPPRGIRAIIEAVRLGEVIKPSQYAKREAFKKHDIKEAWLNRVLTMIFYDIMKKQGLIDKAIKDVVGVTP
LILDPWLRAALRVAFDVVLFHDPNQNTLKNLKWKASDFISSKTHPYVGMYYWDIFERILEYKPNPKTELERLEWEYLAPA
WLIERVRKLLGDETKAFFEAVNRKHEWISIRVNTLKTTPEEVIKEFEEEGVEVVQSKRIPVVLKIKGPYDFDSSYLFRKG
KIIVQEEASAVASVILDPKPGEVVVDLAAAPGGKTTHMAELMKNRGKIFAFDIDKARMKRLKDFVSRMGIKIVKPIVKDA
RKAPEILGEEVADRVMLDAPCTSSGTIGKNPELRWRLREQKIEEMAQLQRELLESAAKLVKPGGRLLYTTCSIFIEEDED
NVSWFLETYKEFRLVKLSGPYDPGFLEGTMRAWPHRHETIGFFYALFEKVTQ
>Mature_452_residues
MEAEKEKKLSIPPRGIRAIIEAVRLGEVIKPSQYAKREAFKKHDIKEAWLNRVLTMIFYDIMKKQGLIDKAIKDVVGVTP
LILDPWLRAALRVAFDVVLFHDPNQNTLKNLKWKASDFISSKTHPYVGMYYWDIFERILEYKPNPKTELERLEWEYLAPA
WLIERVRKLLGDETKAFFEAVNRKHEWISIRVNTLKTTPEEVIKEFEEEGVEVVQSKRIPVVLKIKGPYDFDSSYLFRKG
KIIVQEEASAVASVILDPKPGEVVVDLAAAPGGKTTHMAELMKNRGKIFAFDIDKARMKRLKDFVSRMGIKIVKPIVKDA
RKAPEILGEEVADRVMLDAPCTSSGTIGKNPELRWRLREQKIEEMAQLQRELLESAAKLVKPGGRLLYTTCSIFIEEDED
NVSWFLETYKEFRLVKLSGPYDPGFLEGTMRAWPHRHETIGFFYALFEKVTQ

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=296, Percent_Identity=35.1351351351351, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI76150623, Length=296, Percent_Identity=35.1351351351351, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI270288816, Length=292, Percent_Identity=32.1917808219178, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI8922322, Length=292, Percent_Identity=32.1917808219178, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI23199998, Length=292, Percent_Identity=32.1917808219178, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI270288818, Length=292, Percent_Identity=32.1917808219178, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI32698918, Length=200, Percent_Identity=34.5, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI39995082, Length=185, Percent_Identity=28.6486486486486, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI301336155, Length=185, Percent_Identity=28.6486486486486, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI11545785, Length=184, Percent_Identity=28.804347826087, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI40316918, Length=165, Percent_Identity=31.5151515151515, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI2367212, Length=292, Percent_Identity=32.5342465753425, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081985, Length=284, Percent_Identity=31.3380281690141, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536757, Length=313, Percent_Identity=30.3514376996805, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71998419, Length=303, Percent_Identity=29.7029702970297, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115534021, Length=242, Percent_Identity=31.404958677686, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25143471, Length=273, Percent_Identity=27.1062271062271, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=317, Percent_Identity=32.807570977918, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319447, Length=267, Percent_Identity=26.9662921348315, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324306, Length=342, Percent_Identity=26.3157894736842, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24668781, Length=234, Percent_Identity=34.1880341880342, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356513, Length=234, Percent_Identity=34.1880341880342, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI22024126, Length=288, Percent_Identity=31.25, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356579, Length=226, Percent_Identity=29.2035398230088, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21355201, Length=178, Percent_Identity=28.6516853932584, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920494, Length=167, Percent_Identity=28.7425149700599, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 52213; Mature: 52213

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAEKEKKLSIPPRGIRAIIEAVRLGEVIKPSQYAKREAFKKHDIKEAWLNRVLTMIFYD
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IMKKQGLIDKAIKDVVGVTPLILDPWLRAALRVAFDVVLFHDPNQNTLKNLKWKASDFIS
HHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCHHHHHHC
SKTHPYVGMYYWDIFERILEYKPNPKTELERLEWEYLAPAWLIERVRKLLGDETKAFFEA
CCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
VNRKHEWISIRVNTLKTTPEEVIKEFEEEGVEVVQSKRIPVVLKIKGPYDFDSSYLFRKG
HCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEECC
KIIVQEEASAVASVILDPKPGEVVVDLAAAPGGKTTHMAELMKNRGKIFAFDIDKARMKR
CEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHHHH
LKDFVSRMGIKIVKPIVKDARKAPEILGEEVADRVMLDAPCTSSGTIGKNPELRWRLREQ
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHH
KIEEMAQLQRELLESAAKLVKPGGRLLYTTCSIFIEEDEDNVSWFLETYKEFRLVKLSGP
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCEEEEECCC
YDPGFLEGTMRAWPHRHETIGFFYALFEKVTQ
CCCCHHCCHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEAEKEKKLSIPPRGIRAIIEAVRLGEVIKPSQYAKREAFKKHDIKEAWLNRVLTMIFYD
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IMKKQGLIDKAIKDVVGVTPLILDPWLRAALRVAFDVVLFHDPNQNTLKNLKWKASDFIS
HHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCHHHHHHC
SKTHPYVGMYYWDIFERILEYKPNPKTELERLEWEYLAPAWLIERVRKLLGDETKAFFEA
CCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
VNRKHEWISIRVNTLKTTPEEVIKEFEEEGVEVVQSKRIPVVLKIKGPYDFDSSYLFRKG
HCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCEEECC
KIIVQEEASAVASVILDPKPGEVVVDLAAAPGGKTTHMAELMKNRGKIFAFDIDKARMKR
CEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCHHHHHH
LKDFVSRMGIKIVKPIVKDARKAPEILGEEVADRVMLDAPCTSSGTIGKNPELRWRLREQ
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHH
KIEEMAQLQRELLESAAKLVKPGGRLLYTTCSIFIEEDEDNVSWFLETYKEFRLVKLSGP
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHCEEEEECCC
YDPGFLEGTMRAWPHRHETIGFFYALFEKVTQ
CCCCHHCCHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA