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The map label for this gene is adh1 [H]

Identifier: 18976980

GI number: 18976980

Start: 630051

End: 631178

Strand: Direct

Name: adh1 [H]

Synonym: PF0608

Alternate gene names: 18976980

Gene position: 630051-631178 (Clockwise)

Preceding gene: 18976979

Following gene: 18976981

Centisome position: 33.02

GC content: 45.39

Gene sequence:

>1128_bases
ATGTTTTTCCTAAAGACTAAGATTATCGAGGGAAGGGGAAGTTTGGAGTATCTTCAAAAGGAAGCTATTGGGTTCGAGAG
GGCTTTAATCTTAGCGTCAAGCTCTATGAAAAGGTTAGGGTTTCTGAAGGAAGCTGAAGACTATCTAAGGGAAGGTGGAG
TTGAGGTAATTTCAATAGCAGGCCTTCCTCCTGAGCCCACGAAAGACGTTGTCGACGAGTTCCTCCCAAAAGTTAGGGAG
TTTTCTCCAGAGATCCTCATTGCAATTGGTGGAGGAAGTGTTATCGATATGGCCAAAGCTCTAAAGGTATTTTACGATGT
CCCAGGAATTTCATTTGAGGAAGTTGCAATCAAGAGTAGATTTTCGAAGCCGAGACCCGTTCCAAAGCTGAAAACTCCCC
TTATAGCTATTCCCTCAACGAGCGGTGCTGGAAGTGAGGTTTCAGCAGCGAGCGTGATTAAGGTAGGGGACGTTAAGTAC
ACGATTGTAACTCCCGAGATAGCTCCTGAAGTGGCTATACTTGACCCTAGATTACCCGAAGGAATGCCCCCAGAAGTTGC
CAGGAATTCTGGAATGGATGTTTTAGTTCATGCGATAGAGGCTTATGTTTCAAAGGCTTCCTCACCCTTTACAGATGCAA
TGGCAGTTAAAGCGGCAAAAGCTGTGTTGGAGCATTTAGAGGAGAGCATTAGAGGAGACAAAAATGCCAGAGAGAAAATC
CACTATGCAGCTACAATGGCTGGCATAGCTTTCTTAAACGCAAGGCTTGGACTCGTTCATGCGATGAGTCATAAGGCCGC
ATGGATAGGACCGCATGGTCTCGTAAATGCCATTCTACTTCCCTATGTGATGGAGTATAACATGGCACGCAGGAGAGAGA
AATATGATGCATTGGCGAGGGAATTGGGATTTGACAATGCTGAAGAGCTCCTTGAAGAGATAAAAGCTCTGAACGAAAGG
CTTGGAATAGGACCCATCTCAAGTTACGTCAGCGAGGAGGAGTTCTTAGCGAAGCTTGATGAAATGGTAGAGAGAGCCTA
TGAAGATCCCCTCGTTAACTTCAACCCAGTTGAGCCGAGAAAAGAGGACATTAGAAGAATTTATGAGAGGGCCTTCTATG
GAGATTGA

Upstream 100 bases:

>100_bases
GAAGGTCTACAACAAATTTGGCAAAGAGGTTCCTCTAGAAGAGTTCGTTGAAGAAACTCAGGACTTCATTAAGGAAGTAT
TTGAGAGCTAGGGGATGGTT

Downstream 100 bases:

>100_bases
CAAAGGACTTGCAAAGTTCGGAGATTCGCTGATAAACTTTCTTTACTCTTTAGCACTAACTGAATTCCTGGGAAAGCCCA
CTGGAGATAGAGTTCCAAAT

Product: alcohol dehydrogenase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: BDH [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MFFLKTKIIEGRGSLEYLQKEAIGFERALILASSSMKRLGFLKEAEDYLREGGVEVISIAGLPPEPTKDVVDEFLPKVRE
FSPEILIAIGGGSVIDMAKALKVFYDVPGISFEEVAIKSRFSKPRPVPKLKTPLIAIPSTSGAGSEVSAASVIKVGDVKY
TIVTPEIAPEVAILDPRLPEGMPPEVARNSGMDVLVHAIEAYVSKASSPFTDAMAVKAAKAVLEHLEESIRGDKNAREKI
HYAATMAGIAFLNARLGLVHAMSHKAAWIGPHGLVNAILLPYVMEYNMARRREKYDALARELGFDNAEELLEEIKALNER
LGIGPISSYVSEEEFLAKLDEMVERAYEDPLVNFNPVEPRKEDIRRIYERAFYGD

Sequences:

>Translated_375_residues
MFFLKTKIIEGRGSLEYLQKEAIGFERALILASSSMKRLGFLKEAEDYLREGGVEVISIAGLPPEPTKDVVDEFLPKVRE
FSPEILIAIGGGSVIDMAKALKVFYDVPGISFEEVAIKSRFSKPRPVPKLKTPLIAIPSTSGAGSEVSAASVIKVGDVKY
TIVTPEIAPEVAILDPRLPEGMPPEVARNSGMDVLVHAIEAYVSKASSPFTDAMAVKAAKAVLEHLEESIRGDKNAREKI
HYAATMAGIAFLNARLGLVHAMSHKAAWIGPHGLVNAILLPYVMEYNMARRREKYDALARELGFDNAEELLEEIKALNER
LGIGPISSYVSEEEFLAKLDEMVERAYEDPLVNFNPVEPRKEDIRRIYERAFYGD
>Mature_375_residues
MFFLKTKIIEGRGSLEYLQKEAIGFERALILASSSMKRLGFLKEAEDYLREGGVEVISIAGLPPEPTKDVVDEFLPKVRE
FSPEILIAIGGGSVIDMAKALKVFYDVPGISFEEVAIKSRFSKPRPVPKLKTPLIAIPSTSGAGSEVSAASVIKVGDVKY
TIVTPEIAPEVAILDPRLPEGMPPEVARNSGMDVLVHAIEAYVSKASSPFTDAMAVKAAKAVLEHLEESIRGDKNAREKI
HYAATMAGIAFLNARLGLVHAMSHKAAWIGPHGLVNAILLPYVMEYNMARRREKYDALARELGFDNAEELLEEIKALNER
LGIGPISSYVSEEEFLAKLDEMVERAYEDPLVNFNPVEPRKEDIRRIYERAFYGD

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=329, Percent_Identity=26.7477203647416, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1787493, Length=404, Percent_Identity=35.1485148514851, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789163, Length=378, Percent_Identity=34.3915343915344, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87082107, Length=381, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI48994951, Length=365, Percent_Identity=33.1506849315069, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1789386, Length=307, Percent_Identity=26.7100977198697, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=321, Percent_Identity=26.791277258567, Blast_Score=101, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321181, Length=378, Percent_Identity=32.2751322751323, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24657991, Length=347, Percent_Identity=24.4956772334294, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1

Molecular weight: Translated: 41375; Mature: 41375

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFLKTKIIEGRGSLEYLQKEAIGFERALILASSSMKRLGFLKEAEDYLREGGVEVISIA
CCEEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GLPPEPTKDVVDEFLPKVREFSPEILIAIGGGSVIDMAKALKVFYDVPGISFEEVAIKSR
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FSKPRPVPKLKTPLIAIPSTSGAGSEVSAASVIKVGDVKYTIVTPEIAPEVAILDPRLPE
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHEEEECCEEEEEECCCCCCCEEEECCCCCC
GMPPEVARNSGMDVLVHAIEAYVSKASSPFTDAMAVKAAKAVLEHLEESIRGDKNAREKI
CCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
HYAATMAGIAFLNARLGLVHAMSHKAAWIGPHGLVNAILLPYVMEYNMARRREKYDALAR
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGFDNAEELLEEIKALNERLGIGPISSYVSEEEFLAKLDEMVERAYEDPLVNFNPVEPR
HHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KEDIRRIYERAFYGD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MFFLKTKIIEGRGSLEYLQKEAIGFERALILASSSMKRLGFLKEAEDYLREGGVEVISIA
CCEEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
GLPPEPTKDVVDEFLPKVREFSPEILIAIGGGSVIDMAKALKVFYDVPGISFEEVAIKSR
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FSKPRPVPKLKTPLIAIPSTSGAGSEVSAASVIKVGDVKYTIVTPEIAPEVAILDPRLPE
CCCCCCCCCCCCCEEEECCCCCCCCCCCHHHEEEECCEEEEEECCCCCCCEEEECCCCCC
GMPPEVARNSGMDVLVHAIEAYVSKASSPFTDAMAVKAAKAVLEHLEESIRGDKNAREKI
CCCHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
HYAATMAGIAFLNARLGLVHAMSHKAAWIGPHGLVNAILLPYVMEYNMARRREKYDALAR
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGFDNAEELLEEIKALNERLGIGPISSYVSEEEFLAKLDEMVERAYEDPLVNFNPVEPR
HHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KEDIRRIYERAFYGD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]