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The map label for this gene is argG

Identifier: 18976579

GI number: 18976579

Start: 222845

End: 224077

Strand: Direct

Name: argG

Synonym: PF0207

Alternate gene names: 18976579

Gene position: 222845-224077 (Clockwise)

Preceding gene: 18976578

Following gene: 18976580

Centisome position: 11.68

GC content: 41.69

Gene sequence:

>1233_bases
GTGAGAATAGTGTTGGCTTATTCAGGTGGATTGGATACTTCGGTGATACTAAAACTAATGCAGGAAAAGCTGGGAGCTGA
AGTAATAACTGTTACTGTTGATGTTGGGCAGAAGGATGACTTCGAAAAAATAGAAGAGAAGGCCTACAAGTTTGGTGCCG
TGAAGCACTACTACATAGATGCCAAGGAGGAATTCGCCGAGAACTATGTTTGTAAAGCTATAAAAGCAAATGCTCTTTAC
GAAAATGCGTATCCTCTTTCAACGGCCCTAGCTAGGCCTCTTATAGTAGAAAAGTTAGTTGAGGTTGCAAAGAAGGAGGG
GGCTGGAATAATAGCTCATGGTTGTACAGGAAAGGGAAATGATCAGGTGCGCTTTAACCTAGGAATTAAGGCCTTGATGC
CTGAGGCTGAAATTCTCCAGCCAGTAGCTGAGTGGAACCTTACAAGGGATTGGGAAATGGAGTATGCAAAGAAGCATGGA
ATTCCTGTAAGTGACAAGATATATAGCATAGATGAAAACATCTGGGGAAGGAGCATTGAAGGTGGGGTACTTGAAGATCC
ATCAATAGAACCTCCAGAAGAGGTCTTTGAATGGACAGTATCTATAGAAAAAGCTCCAGACAAACCAGAATATGTGACTA
TTGGCTTTGAAAATGGAGTTCCAGTTTCACTTAATGGGGAAAAAATGAAGCTTCTCGAGCTAATTCTAAAGCTCAACGAA
ATCGCTGGTAAGCATGGGGTTGGAAGGATTGACCATATTGAGGATAGGAGTGTTGGGATTAAGAGCAGGGAGGTTTATGA
AGCCCCTGCGGCTGTTACTCTAATAAAGGCTCATCAGGACTTAGAAAAGTTAACTTTAACAAAGTGGGTTATAGAATTCA
AGAGTATAGTGGACTCTAAGTGGTCTTGGCTTGTATATAATGGCCTATGGTATGAACCACTTAGGTTAGCCTTAGAAGGG
TTCATAGATGAAGCTGAAAAGGCTGTCAACGGCGAAGTTACAGTCAAGCTGTGGAAAGGAAATGCAATTGTCGTTGGAAG
GAAATCGGACAATGCCCTCTACGATGTAAAGATGGCAACTTATGAAAAGTTCAGCACATTTGATCAGAAGCTTGCAAAGG
GATTCATAGAGCTCTTTGGAATGCAGAGTGTTCTAGCTTATAATATGCTTCATGGAGTCCACTCCACCTCAAATATTTCG
GAAATTAAAGAGGCAATAAAGACTTTGGAGTGA

Upstream 100 bases:

>100_bases
TCTGGAGATTAGTTTCATTTGCAACTAATTTTTAATTCTTGAAACATTAATTACAAAACAATTAAAAGACTGTTCTTAAT
GGATCCACTTGGTGATAAAT

Downstream 100 bases:

>100_bases
CATGGTGGGAAATATGTACAGAAAGGCCTTGTTAGGGTCTACTAGGCTTGACATTCTCTCTTACATTTCTTCCATGGAGG
AGGATAGGGAGATTGTTGAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MRIVLAYSGGLDTSVILKLMQEKLGAEVITVTVDVGQKDDFEKIEEKAYKFGAVKHYYIDAKEEFAENYVCKAIKANALY
ENAYPLSTALARPLIVEKLVEVAKKEGAGIIAHGCTGKGNDQVRFNLGIKALMPEAEILQPVAEWNLTRDWEMEYAKKHG
IPVSDKIYSIDENIWGRSIEGGVLEDPSIEPPEEVFEWTVSIEKAPDKPEYVTIGFENGVPVSLNGEKMKLLELILKLNE
IAGKHGVGRIDHIEDRSVGIKSREVYEAPAAVTLIKAHQDLEKLTLTKWVIEFKSIVDSKWSWLVYNGLWYEPLRLALEG
FIDEAEKAVNGEVTVKLWKGNAIVVGRKSDNALYDVKMATYEKFSTFDQKLAKGFIELFGMQSVLAYNMLHGVHSTSNIS
EIKEAIKTLE

Sequences:

>Translated_410_residues
MRIVLAYSGGLDTSVILKLMQEKLGAEVITVTVDVGQKDDFEKIEEKAYKFGAVKHYYIDAKEEFAENYVCKAIKANALY
ENAYPLSTALARPLIVEKLVEVAKKEGAGIIAHGCTGKGNDQVRFNLGIKALMPEAEILQPVAEWNLTRDWEMEYAKKHG
IPVSDKIYSIDENIWGRSIEGGVLEDPSIEPPEEVFEWTVSIEKAPDKPEYVTIGFENGVPVSLNGEKMKLLELILKLNE
IAGKHGVGRIDHIEDRSVGIKSREVYEAPAAVTLIKAHQDLEKLTLTKWVIEFKSIVDSKWSWLVYNGLWYEPLRLALEG
FIDEAEKAVNGEVTVKLWKGNAIVVGRKSDNALYDVKMATYEKFSTFDQKLAKGFIELFGMQSVLAYNMLHGVHSTSNIS
EIKEAIKTLE
>Mature_410_residues
MRIVLAYSGGLDTSVILKLMQEKLGAEVITVTVDVGQKDDFEKIEEKAYKFGAVKHYYIDAKEEFAENYVCKAIKANALY
ENAYPLSTALARPLIVEKLVEVAKKEGAGIIAHGCTGKGNDQVRFNLGIKALMPEAEILQPVAEWNLTRDWEMEYAKKHG
IPVSDKIYSIDENIWGRSIEGGVLEDPSIEPPEEVFEWTVSIEKAPDKPEYVTIGFENGVPVSLNGEKMKLLELILKLNE
IAGKHGVGRIDHIEDRSVGIKSREVYEAPAAVTLIKAHQDLEKLTLTKWVIEFKSIVDSKWSWLVYNGLWYEPLRLALEG
FIDEAEKAVNGEVTVKLWKGNAIVVGRKSDNALYDVKMATYEKFSTFDQKLAKGFIELFGMQSVLAYNMLHGVHSTSNIS
EIKEAIKTLE

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=396, Percent_Identity=40.6565656565657, Blast_Score=279, Evalue=4e-75,
Organism=Homo sapiens, GI16950633, Length=396, Percent_Identity=40.6565656565657, Blast_Score=279, Evalue=4e-75,
Organism=Escherichia coli, GI1789563, Length=388, Percent_Identity=32.4742268041237, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324514, Length=399, Percent_Identity=39.8496240601504, Blast_Score=286, Evalue=5e-78,
Organism=Drosophila melanogaster, GI21358151, Length=393, Percent_Identity=41.4758269720102, Blast_Score=276, Evalue=2e-74,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_PYRFU (Q8U484)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_577936.1
- ProteinModelPortal:   Q8U484
- SMR:   Q8U484
- EnsemblBacteria:   EBPYRT00000005529
- GeneID:   1468039
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0207
- NMPDR:   fig|186497.1.peg.210
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- BRENDA:   6.3.4.5
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45973; Mature: 45973

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 84-84 BINDING 114-114 BINDING 116-116 BINDING 120-120 BINDING 120-120 BINDING 121-121 BINDING 124-124 BINDING 169-169 BINDING 178-178 BINDING 254-254 BINDING 266-266

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVLAYSGGLDTSVILKLMQEKLGAEVITVTVDVGQKDDFEKIEEKAYKFGAVKHYYID
CEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEC
AKEEFAENYVCKAIKANALYENAYPLSTALARPLIVEKLVEVAKKEGAGIIAHGCTGKGN
CHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
DQVRFNLGIKALMPEAEILQPVAEWNLTRDWEMEYAKKHGIPVSDKIYSIDENIWGRSIE
CEEEEEECCHHHCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCC
GGVLEDPSIEPPEEVFEWTVSIEKAPDKPEYVTIGFENGVPVSLNGEKMKLLELILKLNE
CCCCCCCCCCCHHHHHEEEEEEECCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHH
IAGKHGVGRIDHIEDRSVGIKSREVYEAPAAVTLIKAHQDLEKLTLTKWVIEFKSIVDSK
HHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
WSWLVYNGLWYEPLRLALEGFIDEAEKAVNGEVTVKLWKGNAIVVGRKSDNALYDVKMAT
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCEEEEEHHH
YEKFSTFDQKLAKGFIELFGMQSVLAYNMLHGVHSTSNISEIKEAIKTLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRIVLAYSGGLDTSVILKLMQEKLGAEVITVTVDVGQKDDFEKIEEKAYKFGAVKHYYID
CEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCCEEEEEEC
AKEEFAENYVCKAIKANALYENAYPLSTALARPLIVEKLVEVAKKEGAGIIAHGCTGKGN
CHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
DQVRFNLGIKALMPEAEILQPVAEWNLTRDWEMEYAKKHGIPVSDKIYSIDENIWGRSIE
CEEEEEECCHHHCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCC
GGVLEDPSIEPPEEVFEWTVSIEKAPDKPEYVTIGFENGVPVSLNGEKMKLLELILKLNE
CCCCCCCCCCCHHHHHEEEEEEECCCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHH
IAGKHGVGRIDHIEDRSVGIKSREVYEAPAAVTLIKAHQDLEKLTLTKWVIEFKSIVDSK
HHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCC
WSWLVYNGLWYEPLRLALEGFIDEAEKAVNGEVTVKLWKGNAIVVGRKSDNALYDVKMAT
CCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCEEEEEHHH
YEKFSTFDQKLAKGFIELFGMQSVLAYNMLHGVHSTSNISEIKEAIKTLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA