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The map label for this gene is lysK

Identifier: 18976507

GI number: 18976507

Start: 144607

End: 146178

Strand: Direct

Name: lysK

Synonym: PF0135

Alternate gene names: 18976507

Gene position: 144607-146178 (Clockwise)

Preceding gene: 33359455

Following gene: 18976508

Centisome position: 7.58

GC content: 42.56

Gene sequence:

>1572_bases
ATGGTTCACTGGGCTGACTACATGGCTGAAAAGATCATTAAGGAGAGAGGGGAGAAGGAGGAATATGTGGTTGAGAGTGG
TATAACCCCCAGTGGTTACGTTCACGTAGGAAATTTTAGGGAGCTTTTCACTGCTTACATTGTTGGGCATGCATTGAGAG
ATAGAGGATACAATGTTAGACACATTCACATGTGGGATGATTATGATCGTTTTAGAAAAGTTCCCAAGAATGTTCCTCAG
GAGTGGGAAGAGTACTTAGGAATGCCGGTTAGTGAAGTCCCTGACCCCTGGGGATGTCACGATAGCTATGCTGAGCATTT
TATGGGATTATTTGAGGAAGAGGTTGCGAAGCTTGAGATGGATGTAGAGTTCTTGAGGGCTAGTGAGCTCTACAAGAAGG
GGGAATATGCAGAGGAAATTAGGAAGGCCTTTGAGGCTAAGGGGAAGATAATGGCCATACTTAACAAATATAGGGAAGTT
GCTAAGCAACCTCCTCTTCCAGAAAATTGGTGGCCTGCTATGGTGTATTGTCCAGAACACAGAAAGGAAAGTGAGATAAT
AGATTGGGATGGGGAGTGGGGAGTGAAGTATAGATGTCCTGAAGGTCATGAAGGGTGGACAGATATAAGGGATGGCAATG
TAAAGCTTAGGTGGCGTGTTGATTGGCCAATGAGATGGGCACATTTTGGGGTTGATTTTGAGCCTGCAGGAAAGGATCAC
CTAGCCGCTGGTTCAAGTTATGATACAGGGAAAGAAATTATTAGGGAAGTCTATGGAAAAGAAGCCCCACTAACGTTAAT
GTATGAGTTCGTCGGAATTAAGGGACAGAAGGGAAAAATGAGCGGAAGTAAGGGTAACGTAATTCTATTATCTGACTTAT
ATGAAGTCCTTGAACCTGGGCTTGTGAGGTTTATATATGCTAAGCACAGGCCAAACAAGGAGATAAGGATAGACCTTGGT
CTAGGTCTTTTGAATTTGTACGACGAATTCGATAGGGTTGAGAGGATATACTTTGGAATTGAAAAAGGAAAGGGGGATGA
AGAGGAGCTCAAGAGAACTTATGAACTCTCTGTCCCTAAAAAGCCAAAAAGGTTAGTAGCTCAAGCTCCATTTAGATTCT
TAGCTGTACTCGTTCAACTACCCCATCTCTCTATTGAGGATATAATTTTCACATTGGTAAAGCAGGGGCATGTTCCTGAG
AATCTCACCCAAGAGGACATAGATAGAATTAAGCTTAGGATAAAACTGGCCAAAAATTGGGTTGAGAAGTATGCACCAGA
AGAAGTTAAATTTAAGATTCTCAGCGTTCCTGGAGTTTCAGAAGTTGATCCAACTATTAGGGAAGCTATGCTTGAAGTTG
CAGAGTGGCTCGAATCGCATGAAGACTTTGCCGTTGATGAGCTTAACAACATCCTATTTGAGGTTGCCAAGAAGAGGAAT
ATTCCGAGCAAAGTGTGGTTTTCCACATTGTACAAACTATTCATAGGTAAAGATAGAGGGCCAAAGCTAGCTAATTTCTT
AGCTGCATTGGATAGGGAGTTTGTAGTTAGAAGGCTTAGGTTAGAAGGGTGA

Upstream 100 bases:

>100_bases
AAATTAAAAGATGTGAAGCTTAAAAACTGTTTTTCTTAACTATAGGTTGTTGACCGTAACCTTAAAATGATGTTCTCCCA
GCTACCTGTGGTGGTGAAAA

Downstream 100 bases:

>100_bases
ATTTTATGTTGAATGTTGCGGGAATTGGGGTTGAAATTAGGGGAGATTTAGATGATGGGTTTGAGGATGCTTTTCTCTCC
ACTTTTTCTAAAAGATACTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MVHWADYMAEKIIKERGEKEEYVVESGITPSGYVHVGNFRELFTAYIVGHALRDRGYNVRHIHMWDDYDRFRKVPKNVPQ
EWEEYLGMPVSEVPDPWGCHDSYAEHFMGLFEEEVAKLEMDVEFLRASELYKKGEYAEEIRKAFEAKGKIMAILNKYREV
AKQPPLPENWWPAMVYCPEHRKESEIIDWDGEWGVKYRCPEGHEGWTDIRDGNVKLRWRVDWPMRWAHFGVDFEPAGKDH
LAAGSSYDTGKEIIREVYGKEAPLTLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPGLVRFIYAKHRPNKEIRIDLG
LGLLNLYDEFDRVERIYFGIEKGKGDEEELKRTYELSVPKKPKRLVAQAPFRFLAVLVQLPHLSIEDIIFTLVKQGHVPE
NLTQEDIDRIKLRIKLAKNWVEKYAPEEVKFKILSVPGVSEVDPTIREAMLEVAEWLESHEDFAVDELNNILFEVAKKRN
IPSKVWFSTLYKLFIGKDRGPKLANFLAALDREFVVRRLRLEG

Sequences:

>Translated_523_residues
MVHWADYMAEKIIKERGEKEEYVVESGITPSGYVHVGNFRELFTAYIVGHALRDRGYNVRHIHMWDDYDRFRKVPKNVPQ
EWEEYLGMPVSEVPDPWGCHDSYAEHFMGLFEEEVAKLEMDVEFLRASELYKKGEYAEEIRKAFEAKGKIMAILNKYREV
AKQPPLPENWWPAMVYCPEHRKESEIIDWDGEWGVKYRCPEGHEGWTDIRDGNVKLRWRVDWPMRWAHFGVDFEPAGKDH
LAAGSSYDTGKEIIREVYGKEAPLTLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPGLVRFIYAKHRPNKEIRIDLG
LGLLNLYDEFDRVERIYFGIEKGKGDEEELKRTYELSVPKKPKRLVAQAPFRFLAVLVQLPHLSIEDIIFTLVKQGHVPE
NLTQEDIDRIKLRIKLAKNWVEKYAPEEVKFKILSVPGVSEVDPTIREAMLEVAEWLESHEDFAVDELNNILFEVAKKRN
IPSKVWFSTLYKLFIGKDRGPKLANFLAALDREFVVRRLRLEG
>Mature_523_residues
MVHWADYMAEKIIKERGEKEEYVVESGITPSGYVHVGNFRELFTAYIVGHALRDRGYNVRHIHMWDDYDRFRKVPKNVPQ
EWEEYLGMPVSEVPDPWGCHDSYAEHFMGLFEEEVAKLEMDVEFLRASELYKKGEYAEEIRKAFEAKGKIMAILNKYREV
AKQPPLPENWWPAMVYCPEHRKESEIIDWDGEWGVKYRCPEGHEGWTDIRDGNVKLRWRVDWPMRWAHFGVDFEPAGKDH
LAAGSSYDTGKEIIREVYGKEAPLTLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPGLVRFIYAKHRPNKEIRIDLG
LGLLNLYDEFDRVERIYFGIEKGKGDEEELKRTYELSVPKKPKRLVAQAPFRFLAVLVQLPHLSIEDIIFTLVKQGHVPE
NLTQEDIDRIKLRIKLAKNWVEKYAPEEVKFKILSVPGVSEVDPTIREAMLEVAEWLESHEDFAVDELNNILFEVAKKRN
IPSKVWFSTLYKLFIGKDRGPKLANFLAALDREFVVRRLRLEG

Specific function: Unknown

COG id: COG1384

COG function: function code J; Lysyl-tRNA synthetase (class I)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_PYRFU (Q8U4F3)

Other databases:

- EMBL:   AE009950
- RefSeq:   NP_577864.1
- ProteinModelPortal:   Q8U4F3
- SMR:   Q8U4F3
- EnsemblBacteria:   EBPYRT00000004741
- GeneID:   1467967
- GenomeReviews:   AE009950_GR
- KEGG:   pfu:PF0135
- NMPDR:   fig|186497.1.peg.138
- GeneTree:   EBGT00050000023382
- HOGENOM:   HBG297306
- OMA:   VFAVKDW
- ProtClustDB:   PRK00750
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00177
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR002904
- InterPro:   IPR020754
- InterPro:   IPR020756
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- TIGRFAMs:   TIGR00467

Pfam domain/function: PF01921 tRNA-synt_1f; SSF48163 tRNA-synt_bind

EC number: =6.1.1.6

Molecular weight: Translated: 61055; Mature: 61055

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHWADYMAEKIIKERGEKEEYVVESGITPSGYVHVGNFRELFTAYIVGHALRDRGYNVR
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEE
HIHMWDDYDRFRKVPKNVPQEWEEYLGMPVSEVPDPWGCHDSYAEHFMGLFEEEVAKLEM
EEEECCCHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DVEFLRASELYKKGEYAEEIRKAFEAKGKIMAILNKYREVAKQPPLPENWWPAMVYCPEH
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
RKESEIIDWDGEWGVKYRCPEGHEGWTDIRDGNVKLRWRVDWPMRWAHFGVDFEPAGKDH
CCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCCCC
LAAGSSYDTGKEIIREVYGKEAPLTLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPG
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCH
LVRFIYAKHRPNKEIRIDLGLGLLNLYDEFDRVERIYFGIEKGKGDEEELKRTYELSVPK
HHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
KPKRLVAQAPFRFLAVLVQLPHLSIEDIIFTLVKQGHVPENLTQEDIDRIKLRIKLAKNW
CHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
VEKYAPEEVKFKILSVPGVSEVDPTIREAMLEVAEWLESHEDFAVDELNNILFEVAKKRN
HHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
IPSKVWFSTLYKLFIGKDRGPKLANFLAALDREFVVRRLRLEG
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVHWADYMAEKIIKERGEKEEYVVESGITPSGYVHVGNFRELFTAYIVGHALRDRGYNVR
CCCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCEE
HIHMWDDYDRFRKVPKNVPQEWEEYLGMPVSEVPDPWGCHDSYAEHFMGLFEEEVAKLEM
EEEECCCHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DVEFLRASELYKKGEYAEEIRKAFEAKGKIMAILNKYREVAKQPPLPENWWPAMVYCPEH
HHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCC
RKESEIIDWDGEWGVKYRCPEGHEGWTDIRDGNVKLRWRVDWPMRWAHFGVDFEPAGKDH
CCCCCEECCCCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCCCCCCC
LAAGSSYDTGKEIIREVYGKEAPLTLMYEFVGIKGQKGKMSGSKGNVILLSDLYEVLEPG
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCH
LVRFIYAKHRPNKEIRIDLGLGLLNLYDEFDRVERIYFGIEKGKGDEEELKRTYELSVPK
HHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
KPKRLVAQAPFRFLAVLVQLPHLSIEDIIFTLVKQGHVPENLTQEDIDRIKLRIKLAKNW
CHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
VEKYAPEEVKFKILSVPGVSEVDPTIREAMLEVAEWLESHEDFAVDELNNILFEVAKKRN
HHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
IPSKVWFSTLYKLFIGKDRGPKLANFLAALDREFVVRRLRLEG
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA