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The map label for this gene is gidB

Identifier: 18311635

GI number: 18311635

Start: 3024120

End: 3024839

Strand: Reverse

Name: gidB

Synonym: CPE2653

Alternate gene names: 18311635

Gene position: 3024839-3024120 (Counterclockwise)

Preceding gene: 18311636

Following gene: 18311634

Centisome position: 99.78

GC content: 28.61

Gene sequence:

>720_bases
ATGCAATATTTTGATTTAATGAAAAAAGCTTGCGATAGTGTGGGTATGGAATTTAATGAAGACAAATATCAAAAATTTAT
GCTTTACAAAGATTTATTAAAGGAATGGAATGAAAAAATAAATTTAACGGCAATAACAGAGGATGAAGAGATAGTAAAAA
AACATTTTATAGATTGTATAAAAGCTTTTAAAGCAGATGAGTTTAAAAAAGCTAAGACAGTAATAGATGTTGGAACAGGT
GCAGGGTTCCCTGGGTTACCTATAGCTATTATGAGAGAAGATGTAGAGGTTACTCTATTAGACTCATTAAATAAGAGAAT
AAATTTTTTAAATGAAGTTGTAAATAAACTAGGATTAAAAAATGTTAAGACTATACATTCTAGAGCAGAAGATGGTGCAA
GAAAAAAAGAGTTAAGAGAAAATTTTGATATTGCTACATCAAGAGCTGTTGCTAATATGTGTGTTCTAAGTGAATTTTGT
ATTCCATATGTAAAGGTAAATGGGAATTTTATAGCTTTAAAAGGCCCAAATATAACTGAAGAGTTAAATGATTCAAAAAA
TGCTATTGGTACTTTAGGTGGTAAGCTTAAAGGTATAACTGAGGTTGAAATTGAGGGAACAGACTTAAATCATAACTTAG
TAATAGTAGATAAAATAAAAAGTACGCCAAAGACTTTTCCTAGAAAAGCAGGTAATGTAACTAAGAAACCATTAAAATAA

Upstream 100 bases:

>100_bases
TTTAAAAAGTTATCCACATGTGGATAACTTTAGTTTTTTAGTAAAAAATAAAATTATTAAAATAAATTATTTATACTCTT
AAAGTTTTGGAGGATTAAGA

Downstream 100 bases:

>100_bases
ATATAATTTTTAAAATACTTTTTAGTTTAAGAAAATATTATAATTGAAAGTTATAAAAGCTTTTAAAAAATAATAAATAT
ATTGACCATAAAAATAGGAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MQYFDLMKKACDSVGMEFNEDKYQKFMLYKDLLKEWNEKINLTAITEDEEIVKKHFIDCIKAFKADEFKKAKTVIDVGTG
AGFPGLPIAIMREDVEVTLLDSLNKRINFLNEVVNKLGLKNVKTIHSRAEDGARKKELRENFDIATSRAVANMCVLSEFC
IPYVKVNGNFIALKGPNITEELNDSKNAIGTLGGKLKGITEVEIEGTDLNHNLVIVDKIKSTPKTFPRKAGNVTKKPLK

Sequences:

>Translated_239_residues
MQYFDLMKKACDSVGMEFNEDKYQKFMLYKDLLKEWNEKINLTAITEDEEIVKKHFIDCIKAFKADEFKKAKTVIDVGTG
AGFPGLPIAIMREDVEVTLLDSLNKRINFLNEVVNKLGLKNVKTIHSRAEDGARKKELRENFDIATSRAVANMCVLSEFC
IPYVKVNGNFIALKGPNITEELNDSKNAIGTLGGKLKGITEVEIEGTDLNHNLVIVDKIKSTPKTFPRKAGNVTKKPLK
>Mature_239_residues
MQYFDLMKKACDSVGMEFNEDKYQKFMLYKDLLKEWNEKINLTAITEDEEIVKKHFIDCIKAFKADEFKKAKTVIDVGTG
AGFPGLPIAIMREDVEVTLLDSLNKRINFLNEVVNKLGLKNVKTIHSRAEDGARKKELRENFDIATSRAVANMCVLSEFC
IPYVKVNGNFIALKGPNITEELNDSKNAIGTLGGKLKGITEVEIEGTDLNHNLVIVDKIKSTPKTFPRKAGNVTKKPLK

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=202, Percent_Identity=28.7128712871287, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_CLOP1 (Q0TLZ7)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_697343.1
- ProteinModelPortal:   Q0TLZ7
- SMR:   Q0TLZ7
- STRING:   Q0TLZ7
- GeneID:   4203784
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2989
- TIGR:   CPF_2989
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   AITHPNS
- ProtClustDB:   PRK00107
- BioCyc:   CPER195103:CPF_2989-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 26887; Mature: 26887

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYFDLMKKACDSVGMEFNEDKYQKFMLYKDLLKEWNEKINLTAITEDEEIVKKHFIDCI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
KAFKADEFKKAKTVIDVGTGAGFPGLPIAIMREDVEVTLLDSLNKRINFLNEVVNKLGLK
HHHCCHHHHHHHHEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NVKTIHSRAEDGARKKELRENFDIATSRAVANMCVLSEFCIPYVKVNGNFIALKGPNITE
HHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHH
ELNDSKNAIGTLGGKLKGITEVEIEGTDLNHNLVIVDKIKSTPKTFPRKAGNVTKKPLK
HHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQYFDLMKKACDSVGMEFNEDKYQKFMLYKDLLKEWNEKINLTAITEDEEIVKKHFIDCI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
KAFKADEFKKAKTVIDVGTGAGFPGLPIAIMREDVEVTLLDSLNKRINFLNEVVNKLGLK
HHHCCHHHHHHHHEEEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCC
NVKTIHSRAEDGARKKELRENFDIATSRAVANMCVLSEFCIPYVKVNGNFIALKGPNITE
HHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCHH
ELNDSKNAIGTLGGKLKGITEVEIEGTDLNHNLVIVDKIKSTPKTFPRKAGNVTKKPLK
HHCCHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA