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The map label for this gene is pbuO [H]

Identifier: 18311564

GI number: 18311564

Start: 2957466

End: 2958758

Strand: Direct

Name: pbuO [H]

Synonym: CPE2582

Alternate gene names: 18311564

Gene position: 2957466-2958758 (Clockwise)

Preceding gene: 18311556

Following gene: 18311565

Centisome position: 97.56

GC content: 30.39

Gene sequence:

>1293_bases
ATGAATAACTTTTTTAAACTTAAAGAACATGAAACAAATATAAGAACAGAATTACTTGCTGGTCTTACCTCATTTTTTGC
TGCAGTTTATATAATTGTAGTAAATGCTAGCATACTTAGTGATGGTGGAGTATCTATGGAACCACTTATAATAGCTACAG
TATTAGCATCATTAATTGGATGTGTACTAGTTGCACTTTTAAGTAATGCTCCTCTTATTATAATGCCTGGTATGGGTATT
AATGCACTATTTACTTATACCATAGTAAATACTATGGGACTTACATTCCAACAAGCTTTAGCAGCTGTTATAGTAGCTGG
TATTTTGTTTATTGTGGTAGCGGTTACACCTTTAGCAGATATGTTAACTAGCTCAATACCAACCTCATTAAAAGAAGCAA
TAACAGTTGGAATCGGTATTTTTATTACTTTCTTAGGATTACAAAAGGCTGGCTTAATAGTAGGAGATGCCTCAACACTT
GTTAAATTAGGTGATATTTCATCTAAAGAAGTTATAGTATTCTTCATAACTATGATACTAATGCTAGTTTTATTTATAAA
AGATGTACCAGGTTCATTCTTAATAGGAATTTTATCTGGAACAATTATATCAATGTTATTTGGAATTGTTGATTTATCAA
ATTTTCAATTCTCATTACCTAATTTCAGTGAATATAAACAAGTTTTCTTATCAGCTAATTTTAGTGGAATAACTTCAATT
TCATTTTGGATTGCAACCTTCTCACTAACTTTAGTCCTAGTATTTGAAAACATTGGTTTACTTCATGGACAAATTGATGG
TATGTTAAAACAACCAAGTAAAAGACAAAGAGCTCTTAACGCTGTTGCCTTTACTACTATAATTTGTGGTATCTGTGGTA
CTAGTCCTTCAGTTTCAACTGTAGAAGGTAGTGCAGGAATAGCAGCTGGTGGTAAAACAGGATTAACTTCTATATTTACA
GGATTTTTATTTTTAATATCAATCTTCTTTATACCAGTTATATCACTTATTCCAAACTCAGCAATAGCTCCAGTCTTAAT
AATAATTGGAGGCTTAATGATTCAAAATATAGTTTCAATAAACTTTGAAGACTTCACTGAATCATTCCCAGCATTCCTTA
CTTTAACTATAATACCTTTAACATTTAGTATAGTTGATGGTATTGCTTTTGGATTTATAGCTTATCCTATTTGTAAGTTA
GCAACAAAGAAGTATAAAGATGTATCTATCCCTATGTACGTTATATCAGCAATCTTTTTAGTATATTTTGTACTTCACGC
TACACAAATATAG

Upstream 100 bases:

>100_bases
TATTTAAAAAACCTTTATTAAACGTTTAACATATGATATAATAACATTTGCATCGATAGATGTGGGAGGGTGAGGGAGAT
ATTTTTGAAAAGGAGAAAGG

Downstream 100 bases:

>100_bases
CTTTAAATTATAAATTAAACTCCCTTATTAATATCAATAAGGGAGTTTTTTTATATACTGATTATATATATTAAATTAGG
AGTTAATTTTATGGAACTAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNNFFKLKEHETNIRTELLAGLTSFFAAVYIIVVNASILSDGGVSMEPLIIATVLASLIGCVLVALLSNAPLIIMPGMGI
NALFTYTIVNTMGLTFQQALAAVIVAGILFIVVAVTPLADMLTSSIPTSLKEAITVGIGIFITFLGLQKAGLIVGDASTL
VKLGDISSKEVIVFFITMILMLVLFIKDVPGSFLIGILSGTIISMLFGIVDLSNFQFSLPNFSEYKQVFLSANFSGITSI
SFWIATFSLTLVLVFENIGLLHGQIDGMLKQPSKRQRALNAVAFTTIICGICGTSPSVSTVEGSAGIAAGGKTGLTSIFT
GFLFLISIFFIPVISLIPNSAIAPVLIIIGGLMIQNIVSINFEDFTESFPAFLTLTIIPLTFSIVDGIAFGFIAYPICKL
ATKKYKDVSIPMYVISAIFLVYFVLHATQI

Sequences:

>Translated_430_residues
MNNFFKLKEHETNIRTELLAGLTSFFAAVYIIVVNASILSDGGVSMEPLIIATVLASLIGCVLVALLSNAPLIIMPGMGI
NALFTYTIVNTMGLTFQQALAAVIVAGILFIVVAVTPLADMLTSSIPTSLKEAITVGIGIFITFLGLQKAGLIVGDASTL
VKLGDISSKEVIVFFITMILMLVLFIKDVPGSFLIGILSGTIISMLFGIVDLSNFQFSLPNFSEYKQVFLSANFSGITSI
SFWIATFSLTLVLVFENIGLLHGQIDGMLKQPSKRQRALNAVAFTTIICGICGTSPSVSTVEGSAGIAAGGKTGLTSIFT
GFLFLISIFFIPVISLIPNSAIAPVLIIIGGLMIQNIVSINFEDFTESFPAFLTLTIIPLTFSIVDGIAFGFIAYPICKL
ATKKYKDVSIPMYVISAIFLVYFVLHATQI
>Mature_430_residues
MNNFFKLKEHETNIRTELLAGLTSFFAAVYIIVVNASILSDGGVSMEPLIIATVLASLIGCVLVALLSNAPLIIMPGMGI
NALFTYTIVNTMGLTFQQALAAVIVAGILFIVVAVTPLADMLTSSIPTSLKEAITVGIGIFITFLGLQKAGLIVGDASTL
VKLGDISSKEVIVFFITMILMLVLFIKDVPGSFLIGILSGTIISMLFGIVDLSNFQFSLPNFSEYKQVFLSANFSGITSI
SFWIATFSLTLVLVFENIGLLHGQIDGMLKQPSKRQRALNAVAFTTIICGICGTSPSVSTVEGSAGIAAGGKTGLTSIFT
GFLFLISIFFIPVISLIPNSAIAPVLIIIGGLMIQNIVSINFEDFTESFPAFLTLTIIPLTFSIVDGIAFGFIAYPICKL
ATKKYKDVSIPMYVISAIFLVYFVLHATQI

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine. May work at purine concentrations higher than 100 uM [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082309, Length=433, Percent_Identity=33.9491916859122, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1790150, Length=431, Percent_Identity=32.7146171693735, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1790499, Length=436, Percent_Identity=28.6697247706422, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI48994909, Length=432, Percent_Identity=27.3148148148148, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45884; Mature: 45884

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFFKLKEHETNIRTELLAGLTSFFAAVYIIVVNASILSDGGVSMEPLIIATVLASLIG
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
CVLVALLSNAPLIIMPGMGINALFTYTIVNTMGLTFQQALAAVIVAGILFIVVAVTPLAD
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTSSIPTSLKEAITVGIGIFITFLGLQKAGLIVGDASTLVKLGDISSKEVIVFFITMIL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHEECCCCCCHHHHHHHHHHH
MLVLFIKDVPGSFLIGILSGTIISMLFGIVDLSNFQFSLPNFSEYKQVFLSANFSGITSI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCHHHH
SFWIATFSLTLVLVFENIGLLHGQIDGMLKQPSKRQRALNAVAFTTIICGICGTSPSVST
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VEGSAGIAAGGKTGLTSIFTGFLFLISIFFIPVISLIPNSAIAPVLIIIGGLMIQNIVSI
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
NFEDFTESFPAFLTLTIIPLTFSIVDGIAFGFIAYPICKLATKKYKDVSIPMYVISAIFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VYFVLHATQI
HHHHHHHHCC
>Mature Secondary Structure
MNNFFKLKEHETNIRTELLAGLTSFFAAVYIIVVNASILSDGGVSMEPLIIATVLASLIG
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
CVLVALLSNAPLIIMPGMGINALFTYTIVNTMGLTFQQALAAVIVAGILFIVVAVTPLAD
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLTSSIPTSLKEAITVGIGIFITFLGLQKAGLIVGDASTLVKLGDISSKEVIVFFITMIL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHEECCCCCCHHHHHHHHHHH
MLVLFIKDVPGSFLIGILSGTIISMLFGIVDLSNFQFSLPNFSEYKQVFLSANFSGITSI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHCCCCCHHHH
SFWIATFSLTLVLVFENIGLLHGQIDGMLKQPSKRQRALNAVAFTTIICGICGTSPSVST
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VEGSAGIAAGGKTGLTSIFTGFLFLISIFFIPVISLIPNSAIAPVLIIIGGLMIQNIVSI
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
NFEDFTESFPAFLTLTIIPLTFSIVDGIAFGFIAYPICKLATKKYKDVSIPMYVISAIFL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VYFVLHATQI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 11591660 [H]