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The map label for this gene is glnA [H]

Identifier: 18311551

GI number: 18311551

Start: 2940944

End: 2942845

Strand: Direct

Name: glnA [H]

Synonym: CPE2569

Alternate gene names: 18311551

Gene position: 2940944-2942845 (Clockwise)

Preceding gene: 18311541

Following gene: 18311556

Centisome position: 97.02

GC content: 29.86

Gene sequence:

>1902_bases
ATGAATAAAGATTTATTATTTACAATTCCTAAGAAATATCATAATAAAGAAAGTTTAATTTCTATTTTTGAGGACCATCC
TGAAATAAAATTTGTATCTTTAGTTGGGGTTGACTTAGCAGGTAATGATACTGACGAAAAAATTCCTTCTAAACTATTTT
TAAAAGATATAGATTCTTTCCTTCATGGAACTGCCGTACAAACAGACGGTTCTTCAGTTGTCCTTCCAGGAATAGCAACT
TTAAATAACGCAAAAGTGGATATGATTGCCGATTTAGATTGTAAGTGGTTTGTAGATTACAATTATGATTTTATAGACCC
TTTAACTAATAGACCAGTAGGAACACTTAGAATTCCTTGTTTCTTAATCCATGAAGATAAAGCAGTGGACTCTAGACATA
TATTAAAAAATTCAATTGACACATTCAAATCAAACATATTCTCATTATTAAAAAAATATCCTGAAACACTAAATGATTTA
AATATAAAATTCGATGATATAGATGACCTTTTATTAACATCTGCAACAGAATTAGAATTTTGGGTAAAAACACCAAATGA
TAAAGCTTTAATTGAAGAACTTTCAACTTCTGAAGTTTTACAAGAACAATATTGGACAAGAACTAAGGGTAATGTTAGAA
CAGCCTTAGAACAAAGTTTATTATATATGGATGAATATGGATTTGAACCTGAAATGGGACACAAAGAGGTTGGTGGAGTT
AAAGCTAAGCTTACTTCTTCAGGTCAATTTGATCATATAATGGAACAATTAGAAATAGACTGGAAATTCTCAGATGCTAT
GCAAGCTGCAGATAATGAATTATTTATAAGAACATTAGTTAAAGAAACTTTTAGAAGAAACGGATTAGAAGTAACCTTCC
TTGCTAAACCAATATCAGGAGTTGCTGGTAGTGGAGAACACACTCATTTAAGCTTAAGCTTAAAACTAAAAGATGGAAGA
ATGATAAATTTATTTAATCCAACTAAAAATCATTTCTTAAGTAAAATAGGGTATGGTTCTATAATGGGTATATTAAAGAA
CTACGAGGTAATGAATCCATTTATCTCAGCTACAACAGACTCATTAAAAAGATTAAAACCAGGATTTGAAGCTCCTGTTT
GTATAGTTACATCTCTAGGTCAAAGCCCTGAAGTTCCATCTAGAAATAGAACTATATTAGCAGGTTTAATAAGAGATCCT
CACAATCCTTTAGCTACTAGATTTGAACTTAGATCACCAAATCCATTTACAAATACTTATCTTTGTATAGCATCAAGCTA
TATGGCTATGTTAGATGGTATTAAGTATGCTTTAGAAAATGATAAAACAGAAGATGATCTTTTAGCTGAATTATCTAAAA
AACCTGGTGAAGAAGCAGATTACCTAGAAAAATCTAGAGCATATAGAAGTGAAGAAGATGTGTTTGAAGATTTCACAGAT
TCACAAAGAGATGAATACTTTGGTGTGGCTCCTGCTACTGTTTTTGAAAACCTAAGTGCTTTTGATAAATACCCTGAAAA
GGTAGATGTATTAAAAGTAAATTCAGTATTTACAGACAAGCTTATAAATAGTTTCAAAATGGCTACTACTAAGAGATGGA
CTACAGAAATAACTTCAAGAATAATACCTTCTTACACAAAAGATATAAGAGCCGCTAAACAACTTCATTGTTGTGATAAA
GCCTTAGATCTTGACGTATCAACTTGGATGACAATAAATGAATTAAGACATATAACAATGAAAGATTCTTATCACAGAAG
AAGCTTATTTACTCAAATAAAGAATGCTATAAATGAATCTGATTTTGAAAAAGCTTCTGATTTACAAATAAAACTTGATA
AAAACATGAGTGAATTAAATGACCTTTACTCAACTTACAAGAAAAATTTATTAGATATATAA

Upstream 100 bases:

>100_bases
TACGTTTAAAATAACTATTAGTCATAAAAATGAAAGGATGTGAACAGAAGATTATTGCAATTATGTTAATTTATTATAAT
TGTATTACTTCTAAATAATT

Downstream 100 bases:

>100_bases
TAAAAAAAGAAATCAATGCTTTAAATTGATTTCTCATACTTTAAAGAAATGACCTTTGAAATTAACAAAGGTCATTTTAT
ATTCTTATATTAATGATATG

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MNKDLLFTIPKKYHNKESLISIFEDHPEIKFVSLVGVDLAGNDTDEKIPSKLFLKDIDSFLHGTAVQTDGSSVVLPGIAT
LNNAKVDMIADLDCKWFVDYNYDFIDPLTNRPVGTLRIPCFLIHEDKAVDSRHILKNSIDTFKSNIFSLLKKYPETLNDL
NIKFDDIDDLLLTSATELEFWVKTPNDKALIEELSTSEVLQEQYWTRTKGNVRTALEQSLLYMDEYGFEPEMGHKEVGGV
KAKLTSSGQFDHIMEQLEIDWKFSDAMQAADNELFIRTLVKETFRRNGLEVTFLAKPISGVAGSGEHTHLSLSLKLKDGR
MINLFNPTKNHFLSKIGYGSIMGILKNYEVMNPFISATTDSLKRLKPGFEAPVCIVTSLGQSPEVPSRNRTILAGLIRDP
HNPLATRFELRSPNPFTNTYLCIASSYMAMLDGIKYALENDKTEDDLLAELSKKPGEEADYLEKSRAYRSEEDVFEDFTD
SQRDEYFGVAPATVFENLSAFDKYPEKVDVLKVNSVFTDKLINSFKMATTKRWTTEITSRIIPSYTKDIRAAKQLHCCDK
ALDLDVSTWMTINELRHITMKDSYHRRSLFTQIKNAINESDFEKASDLQIKLDKNMSELNDLYSTYKKNLLDI

Sequences:

>Translated_633_residues
MNKDLLFTIPKKYHNKESLISIFEDHPEIKFVSLVGVDLAGNDTDEKIPSKLFLKDIDSFLHGTAVQTDGSSVVLPGIAT
LNNAKVDMIADLDCKWFVDYNYDFIDPLTNRPVGTLRIPCFLIHEDKAVDSRHILKNSIDTFKSNIFSLLKKYPETLNDL
NIKFDDIDDLLLTSATELEFWVKTPNDKALIEELSTSEVLQEQYWTRTKGNVRTALEQSLLYMDEYGFEPEMGHKEVGGV
KAKLTSSGQFDHIMEQLEIDWKFSDAMQAADNELFIRTLVKETFRRNGLEVTFLAKPISGVAGSGEHTHLSLSLKLKDGR
MINLFNPTKNHFLSKIGYGSIMGILKNYEVMNPFISATTDSLKRLKPGFEAPVCIVTSLGQSPEVPSRNRTILAGLIRDP
HNPLATRFELRSPNPFTNTYLCIASSYMAMLDGIKYALENDKTEDDLLAELSKKPGEEADYLEKSRAYRSEEDVFEDFTD
SQRDEYFGVAPATVFENLSAFDKYPEKVDVLKVNSVFTDKLINSFKMATTKRWTTEITSRIIPSYTKDIRAAKQLHCCDK
ALDLDVSTWMTINELRHITMKDSYHRRSLFTQIKNAINESDFEKASDLQIKLDKNMSELNDLYSTYKKNLLDI
>Mature_633_residues
MNKDLLFTIPKKYHNKESLISIFEDHPEIKFVSLVGVDLAGNDTDEKIPSKLFLKDIDSFLHGTAVQTDGSSVVLPGIAT
LNNAKVDMIADLDCKWFVDYNYDFIDPLTNRPVGTLRIPCFLIHEDKAVDSRHILKNSIDTFKSNIFSLLKKYPETLNDL
NIKFDDIDDLLLTSATELEFWVKTPNDKALIEELSTSEVLQEQYWTRTKGNVRTALEQSLLYMDEYGFEPEMGHKEVGGV
KAKLTSSGQFDHIMEQLEIDWKFSDAMQAADNELFIRTLVKETFRRNGLEVTFLAKPISGVAGSGEHTHLSLSLKLKDGR
MINLFNPTKNHFLSKIGYGSIMGILKNYEVMNPFISATTDSLKRLKPGFEAPVCIVTSLGQSPEVPSRNRTILAGLIRDP
HNPLATRFELRSPNPFTNTYLCIASSYMAMLDGIKYALENDKTEDDLLAELSKKPGEEADYLEKSRAYRSEEDVFEDFTD
SQRDEYFGVAPATVFENLSAFDKYPEKVDVLKVNSVFTDKLINSFKMATTKRWTTEITSRIIPSYTKDIRAAKQLHCCDK
ALDLDVSTWMTINELRHITMKDSYHRRSLFTQIKNAINESDFEKASDLQIKLDKNMSELNDLYSTYKKNLLDI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=471, Percent_Identity=28.6624203821656, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 72107; Mature: 72107

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDLLFTIPKKYHNKESLISIFEDHPEIKFVSLVGVDLAGNDTDEKIPSKLFLKDIDSF
CCCCEEEECCHHHCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHH
LHGTAVQTDGSSVVLPGIATLNNAKVDMIADLDCKWFVDYNYDFIDPLTNRPVGTLRIPC
HCCCEEECCCCEEEECCCEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEE
FLIHEDKAVDSRHILKNSIDTFKSNIFSLLKKYPETLNDLNIKFDDIDDLLLTSATELEF
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHCCCCCEEE
WVKTPNDKALIEELSTSEVLQEQYWTRTKGNVRTALEQSLLYMDEYGFEPEMGHKEVGGV
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCE
KAKLTSSGQFDHIMEQLEIDWKFSDAMQAADNELFIRTLVKETFRRNGLEVTFLAKPISG
EEEECCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
VAGSGEHTHLSLSLKLKDGRMINLFNPTKNHFLSKIGYGSIMGILKNYEVMNPFISATTD
CCCCCCCEEEEEEEEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHH
SLKRLKPGFEAPVCIVTSLGQSPEVPSRNRTILAGLIRDPHNPLATRFELRSPNPFTNTY
HHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCHH
LCIASSYMAMLDGIKYALENDKTEDDLLAELSKKPGEEADYLEKSRAYRSEEDVFEDFTD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCC
SQRDEYFGVAPATVFENLSAFDKYPEKVDVLKVNSVFTDKLINSFKMATTKRWTTEITSR
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IIPSYTKDIRAAKQLHCCDKALDLDVSTWMTINELRHITMKDSYHRRSLFTQIKNAINES
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHHHHHCCCHHHHHHHHHHHHHHCHH
DFEKASDLQIKLDKNMSELNDLYSTYKKNLLDI
HCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKDLLFTIPKKYHNKESLISIFEDHPEIKFVSLVGVDLAGNDTDEKIPSKLFLKDIDSF
CCCCEEEECCHHHCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHH
LHGTAVQTDGSSVVLPGIATLNNAKVDMIADLDCKWFVDYNYDFIDPLTNRPVGTLRIPC
HCCCEEECCCCEEEECCCEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEEEE
FLIHEDKAVDSRHILKNSIDTFKSNIFSLLKKYPETLNDLNIKFDDIDDLLLTSATELEF
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHCCCCCEEE
WVKTPNDKALIEELSTSEVLQEQYWTRTKGNVRTALEQSLLYMDEYGFEPEMGHKEVGGV
EEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCE
KAKLTSSGQFDHIMEQLEIDWKFSDAMQAADNELFIRTLVKETFRRNGLEVTFLAKPISG
EEEECCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCCC
VAGSGEHTHLSLSLKLKDGRMINLFNPTKNHFLSKIGYGSIMGILKNYEVMNPFISATTD
CCCCCCCEEEEEEEEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHH
SLKRLKPGFEAPVCIVTSLGQSPEVPSRNRTILAGLIRDPHNPLATRFELRSPNPFTNTY
HHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCHH
LCIASSYMAMLDGIKYALENDKTEDDLLAELSKKPGEEADYLEKSRAYRSEEDVFEDFTD
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCC
SQRDEYFGVAPATVFENLSAFDKYPEKVDVLKVNSVFTDKLINSFKMATTKRWTTEITSR
CCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IIPSYTKDIRAAKQLHCCDKALDLDVSTWMTINELRHITMKDSYHRRSLFTQIKNAINES
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHHHHHCCCHHHHHHHHHHHHHHCHH
DFEKASDLQIKLDKNMSELNDLYSTYKKNLLDI
HCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]