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The map label for this gene is tilS [H]

Identifier: 18311454

GI number: 18311454

Start: 2829427

End: 2830836

Strand: Reverse

Name: tilS [H]

Synonym: CPE2472

Alternate gene names: 18311454

Gene position: 2830836-2829427 (Counterclockwise)

Preceding gene: 18311455

Following gene: 18311453

Centisome position: 93.38

GC content: 25.25

Gene sequence:

>1410_bases
ATGAAGAAAAAGGTTATAGATTTTATAAAGGAAAACTCCATGATTAAAAGTGGAGATAAGGTTTTAGTAGCTTTATCAGG
TGGTCCAGACTCAGTATGTTTACTACATATTTTAAGTGAACTTAGGGAATTATTACACATAGAAGTATATGCAGCCCATG
TGAATCATTGTTTAAGAGGAGAAAGTGCTTTAAAAGATGAAGCTTATGTAGAAGAACTTTGTAAGAATTTAAATATAAAA
TGTTTTGTAAAAAGAGTAGATATAAACAAGATAAGTGAAGAACAAAACATTTCTACAGAAATGGCTGGAAGAGAAGAGAG
ATATAAATTCTTTGAAGAACTAAAAAATGAATATTCATTAGATAAAATAGCTATAGCACACAATGCTAATGATCAAGCAG
AAACTCTTATTATGAGAGCTTTAAGAGGAACTGGTATAGAAGGATTAGTTGGAATTAAACCAGTTAGAGATGGTATTTTT
ATAAGGCCTATATTGATTTTAAGAAGAAAAGAAATAGAAGAATACTGTGAAATAAATAATTTAAATCCTAGAATAGATGA
AACTAATTTAGAAGAAATATATTCTAGAAATAAAATAAGGCTTAAGGCAATACCATTTATAGAAGATAATTTTAATCCAG
ATATAGTTGCTACTTTAAATAGATTAGCTTATAGCTGTAGTAAGGATGTAGAGTTTATACAAGAAGAAGTGGAAAAGAGA
TTTCCTAAACTTTGTATAAAAGAAAATCACAGCATTCTTATTAAAGAAGAAGCCTTTAATGAAAAGGAAGCTCTATTAAC
AAGAATAATAAAAAAGGCATTATTTGAAGTAAGTTCTAAACATAATAACTTTGAATTAAAACATATACAGGACATAATTG
CCTTAAAAGATAAAGGGACAGGAAAGCAAATTAACATAACTAATGGGGTAATTGCTTTAAATGAATATGGAGATATAAGA
ATAAAATTAGTAGATAGCAAAAAGGCTAAAGAGAATAAAGTTTTAAATTTAGAAAATATTAAAGATGAACTAGATAAAAA
TCAAAAAGTTGTAATAGAAGATGATATTTTAGGAAATTATGAATTAATAGTAGAAGATTTAAAAAAAGGTGAAAAATTTT
CAAAAGATAGATTTATAAAGTCTTTTGATTATGATAAAATTTCCAATATAGATATTAGATTTAGACAAAATGGAGATAAA
ATAATTCCTTTAGGAATGAAGAGTAGCAAAAAGCTAAAAGATATATTTATAAATAATAAAATACCTAAAGAAGAAAGAGA
TTTTATACCTTTAGTGCTATTTAATAATGAAATAGCATGGATTGTAGGATCTAATGTTAGTGAAACATTTAAAGTTACAA
ATAAAACTAAAAAGGTAATTAAAATTACTTTTAAAGGAAAGGAAAATTAA

Upstream 100 bases:

>100_bases
ATTATTTAAAATTAATTATACGAACTTTTTTAAGAACATAAGGGATTTTTTATGTGTTATAATATTAGGGCAAAATATAA
TATTTATGGAGTGAGTTACA

Downstream 100 bases:

>100_bases
TTATGATAAGAGACATAAAAGATGATGTTAAAAAAGTTTTATATTCAGAAGAACAATTAGCTAAACGTGTAAAAGAGATG
GCTGAAGAAATTAGTAAAGA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKKKVIDFIKENSMIKSGDKVLVALSGGPDSVCLLHILSELRELLHIEVYAAHVNHCLRGESALKDEAYVEELCKNLNIK
CFVKRVDINKISEEQNISTEMAGREERYKFFEELKNEYSLDKIAIAHNANDQAETLIMRALRGTGIEGLVGIKPVRDGIF
IRPILILRRKEIEEYCEINNLNPRIDETNLEEIYSRNKIRLKAIPFIEDNFNPDIVATLNRLAYSCSKDVEFIQEEVEKR
FPKLCIKENHSILIKEEAFNEKEALLTRIIKKALFEVSSKHNNFELKHIQDIIALKDKGTGKQINITNGVIALNEYGDIR
IKLVDSKKAKENKVLNLENIKDELDKNQKVVIEDDILGNYELIVEDLKKGEKFSKDRFIKSFDYDKISNIDIRFRQNGDK
IIPLGMKSSKKLKDIFINNKIPKEERDFIPLVLFNNEIAWIVGSNVSETFKVTNKTKKVIKITFKGKEN

Sequences:

>Translated_469_residues
MKKKVIDFIKENSMIKSGDKVLVALSGGPDSVCLLHILSELRELLHIEVYAAHVNHCLRGESALKDEAYVEELCKNLNIK
CFVKRVDINKISEEQNISTEMAGREERYKFFEELKNEYSLDKIAIAHNANDQAETLIMRALRGTGIEGLVGIKPVRDGIF
IRPILILRRKEIEEYCEINNLNPRIDETNLEEIYSRNKIRLKAIPFIEDNFNPDIVATLNRLAYSCSKDVEFIQEEVEKR
FPKLCIKENHSILIKEEAFNEKEALLTRIIKKALFEVSSKHNNFELKHIQDIIALKDKGTGKQINITNGVIALNEYGDIR
IKLVDSKKAKENKVLNLENIKDELDKNQKVVIEDDILGNYELIVEDLKKGEKFSKDRFIKSFDYDKISNIDIRFRQNGDK
IIPLGMKSSKKLKDIFINNKIPKEERDFIPLVLFNNEIAWIVGSNVSETFKVTNKTKKVIKITFKGKEN
>Mature_469_residues
MKKKVIDFIKENSMIKSGDKVLVALSGGPDSVCLLHILSELRELLHIEVYAAHVNHCLRGESALKDEAYVEELCKNLNIK
CFVKRVDINKISEEQNISTEMAGREERYKFFEELKNEYSLDKIAIAHNANDQAETLIMRALRGTGIEGLVGIKPVRDGIF
IRPILILRRKEIEEYCEINNLNPRIDETNLEEIYSRNKIRLKAIPFIEDNFNPDIVATLNRLAYSCSKDVEFIQEEVEKR
FPKLCIKENHSILIKEEAFNEKEALLTRIIKKALFEVSSKHNNFELKHIQDIIALKDKGTGKQINITNGVIALNEYGDIR
IKLVDSKKAKENKVLNLENIKDELDKNQKVVIEDDILGNYELIVEDLKKGEKFSKDRFIKSFDYDKISNIDIRFRQNGDK
IIPLGMKSSKKLKDIFINNKIPKEERDFIPLVLFNNEIAWIVGSNVSETFKVTNKTKKVIKITFKGKEN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=224, Percent_Identity=29.0178571428571, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54139; Mature: 54139

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVIDFIKENSMIKSGDKVLVALSGGPDSVCLLHILSELRELLHIEVYAAHVNHCLRG
CCCHHHHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ESALKDEAYVEELCKNLNIKCFVKRVDINKISEEQNISTEMAGREERYKFFEELKNEYSL
CHHCCHHHHHHHHHHCCCEEEEEEEECCHHCCCHHCCCHHHCCHHHHHHHHHHHHHHCCC
DKIAIAHNANDQAETLIMRALRGTGIEGLVGIKPVRDGIFIRPILILRRKEIEEYCEINN
CEEEEEECCCHHHHHHHHHHHHCCCCCCCEECEECCCCEEEHHHHHHHHHHHHHHHCCCC
LNPRIDETNLEEIYSRNKIRLKAIPFIEDNFNPDIVATLNRLAYSCSKDVEFIQEEVEKR
CCCCCCCCCHHHHHCCCCEEEEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FPKLCIKENHSILIKEEAFNEKEALLTRIIKKALFEVSSKHNNFELKHIQDIIALKDKGT
CCHHEECCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCC
GKQINITNGVIALNEYGDIRIKLVDSKKAKENKVLNLENIKDELDKNQKVVIEDDILGNY
CCEEEECCCEEEEECCCCEEEEEECCCCCCCCCCEEHHHHHHHHCCCCEEEEECCCCCCH
ELIVEDLKKGEKFSKDRFIKSFDYDKISNIDIRFRQNGDKIIPLGMKSSKKLKDIFINNK
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHCCCC
IPKEERDFIPLVLFNNEIAWIVGSNVSETFKVTNKTKKVIKITFKGKEN
CCCCCCCEEEEEEECCEEEEEECCCCCHHHHCCCCCEEEEEEEEECCCC
>Mature Secondary Structure
MKKKVIDFIKENSMIKSGDKVLVALSGGPDSVCLLHILSELRELLHIEVYAAHVNHCLRG
CCCHHHHHHHCCCEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ESALKDEAYVEELCKNLNIKCFVKRVDINKISEEQNISTEMAGREERYKFFEELKNEYSL
CHHCCHHHHHHHHHHCCCEEEEEEEECCHHCCCHHCCCHHHCCHHHHHHHHHHHHHHCCC
DKIAIAHNANDQAETLIMRALRGTGIEGLVGIKPVRDGIFIRPILILRRKEIEEYCEINN
CEEEEEECCCHHHHHHHHHHHHCCCCCCCEECEECCCCEEEHHHHHHHHHHHHHHHCCCC
LNPRIDETNLEEIYSRNKIRLKAIPFIEDNFNPDIVATLNRLAYSCSKDVEFIQEEVEKR
CCCCCCCCCHHHHHCCCCEEEEEECEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FPKLCIKENHSILIKEEAFNEKEALLTRIIKKALFEVSSKHNNFELKHIQDIIALKDKGT
CCHHEECCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCC
GKQINITNGVIALNEYGDIRIKLVDSKKAKENKVLNLENIKDELDKNQKVVIEDDILGNY
CCEEEECCCEEEEECCCCEEEEEECCCCCCCCCCEEHHHHHHHHCCCCEEEEECCCCCCH
ELIVEDLKKGEKFSKDRFIKSFDYDKISNIDIRFRQNGDKIIPLGMKSSKKLKDIFINNK
HHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHCCCC
IPKEERDFIPLVLFNNEIAWIVGSNVSETFKVTNKTKKVIKITFKGKEN
CCCCCCCEEEEEEECCEEEEEECCCCCHHHHCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA