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The map label for this gene is ispF

Identifier: 18311298

GI number: 18311298

Start: 2663009

End: 2663479

Strand: Direct

Name: ispF

Synonym: CPE2316

Alternate gene names: 18311298

Gene position: 2663009-2663479 (Clockwise)

Preceding gene: 18311294

Following gene: 18311302

Centisome position: 87.85

GC content: 30.79

Gene sequence:

>471_bases
ATGAGAATAGGAATGGGATATGATGTTCATAAATTAGTTGAAAATAGAGATTTAATATTAGGTGGAGTAAAAATTCCTTA
TGAAAAAGGACTTTTAGGCCATTCAGATGCTGATGTATTATTACACGCAATAATGGATTCACTATTAGGAGCAGCGGCTT
TAGGAGATATAGGAAAGCATTTTCCAGATACAGACCCTAAATACAAAGGTGCTGATAGCATAAAACTTCTAGAATTTGTA
GGTGAACTTTTAAATAAAAATAACTATAAAATATCAAATATAGATGCAACTATAATAGCTCAAAGACCAAAAATGGCTCC
TCATATACCTACTATGAGAGAAAATATAGCTAAAGCATTAAATATAGACTTAGATCAAATAAATGTTAAAGCAACTACTG
AGGAAGGACTTGGTTTTACTGGTTCTGGAGAAGGAATCTCATCTCAAAGTATTTGTCTTTTAGTAAAATAA

Upstream 100 bases:

>100_bases
TTTAATAGCTATTGATGCTTTGATAACATAACAGAATTATATATAATTAAACCATAAAAATAAATTTTCATTGAAAAAAT
AAATCTAAGAGGTGATAAAA

Downstream 100 bases:

>100_bases
AAAAATAGGCCCAATTAATAAAACTTACAAACATATCTTGAGAATTATTAAATATCCTCTTCATATTGTTTGTAAGTTTT
ATTTTCTATAGCCTATCTTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFV
GELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLVK

Sequences:

>Translated_156_residues
MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFV
GELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLVK
>Mature_156_residues
MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKHFPDTDPKYKGADSIKLLEFV
GELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKALNIDLDQINVKATTEEGLGFTGSGEGISSQSICLLVK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=60.2564102564103, Blast_Score=186, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CLOP1 (Q0TMY4)

Other databases:

- EMBL:   CP000246
- RefSeq:   YP_697006.1
- ProteinModelPortal:   Q0TMY4
- SMR:   Q0TMY4
- STRING:   Q0TMY4
- GeneID:   4203168
- GenomeReviews:   CP000246_GR
- KEGG:   cpf:CPF_2616
- TIGR:   CPF_2616
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   CPER195103:CPF_2616-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16866; Mature: 16866

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEEECEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FPDTDPKYKGADSIKLLEFVGELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKAL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
NIDLDQINVKATTEEGLGFTGSGEGISSQSICLLVK
CCCHHHEEEEEECCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MRIGMGYDVHKLVENRDLILGGVKIPYEKGLLGHSDADVLLHAIMDSLLGAAALGDIGKH
CCCCCCCCHHHHHCCCCEEEECEECCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FPDTDPKYKGADSIKLLEFVGELLNKNNYKISNIDATIIAQRPKMAPHIPTMRENIAKAL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
NIDLDQINVKATTEEGLGFTGSGEGISSQSICLLVK
CCCHHHEEEEEECCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA