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The map label for this gene is narK [H]

Identifier: 18311256

GI number: 18311256

Start: 2613396

End: 2614688

Strand: Direct

Name: narK [H]

Synonym: CPE2274

Alternate gene names: 18311256

Gene position: 2613396-2614688 (Clockwise)

Preceding gene: 18311240

Following gene: 18311273

Centisome position: 86.21

GC content: 33.72

Gene sequence:

>1293_bases
ATGAAAAACTTAAATCTTAAAGGTAATCCAAATCGTGGATTAATAGGAGCTACATTAGGTTTCTTCATAGGTTTTGCAGC
AGTTTCTCTTTATGGACCAACTTCTGCAGTATTTAAAGAAGCTTTCGTAAACCTTAATCCAGTATTATTAGCATTACTTA
TTGCAGCCCCTAACTTATCAGGTTCTTTACTTAGAATACCTTTTTCAGCATGGGTTGACACAACTGGTGGGAGAAAACCA
CTTATAGTTTTACTACTTTTATCAATAATAGGTATGGGTGGTTTATACATAGTATTAGCTTTCTTTAGCGATAACTTAAA
TCAATATTATTATTTATTATTTGCACTTGGACTATTATCTGGATGTGGTATAGCAACATTCTCTGTTGGTGTCAGCCAAG
CATCATATTGGTTCCCAAAAAGCAAACAAGGTGTAGCTTTAGGAATATATGGTGGTGTTGGTAACTTAGCACCTGGAATA
TTTGCATTATTAATTCCAAACTTAGCACTTCCTCTATTAGGATTACCTGGTTCTTACTTAGCTTGGTTAATATTCTTAAT
AGTTGGTACTGTAATTTACATAAAAATAGGTCAAAACGCTTGGTACTTCCAACTTGTTGACAAAGGAATAAATAAAGATG
AAGCTAAGGAAATAGCATCAAAAGAGTATGGTCAAGAATTATTCCCTAAGGGAAAAGCAAGTGAAACACTTTTAATATCA
GCTAAGTCATGGAAAACTTGGGCTTTAGTATTTATCTATTTCACAACTTTCGGTGGATTCTTAGCATTAACAGGTTGGTT
CCCTAACTACTGGATGACTTATTTCGGATTAAATATGAAAGTTGCAGGTCTTTTAACAGCTTTATATTCTATATTAACTT
CATTAACTAGAATATATGGTGGAAAAGTTGCTGATAAAAATGGTGGAGAAATAACTACTATAGTTTCTCTAGGAATAGCA
CTTATCGGTGCAATTTGTATGACTTTTGCTTCAACTATGACTTTAGCAATAATAGGTATAATCTTACTTGCTATTGGTAT
GGGAGTTGCAAACGCAGCAGTATTTAAAATAGTTCCTAACGCAGTTCCAGAGGCAATGGGTGGAGCTTCAGGATGGATTG
GTGGATTAGGTGCTTTAGGTGGATTCTTAATACCTCCTGTAATGGCATCATTCCTAAACAGATCAGGATTTGCTGGATAT
TCACAAGGATTTTCAGTATTTATAGTTTTAATAATTGTAGCAATTGGAGTAATAGCTTTATTCCAAGCTTTACAAAAGAA
ATCAGCAAAATAA

Upstream 100 bases:

>100_bases
TATTCTTTTATTTGATGATATACTTTCAAAGTATTTATTAGATAATTATAAATTTCAGAGTTAAAAAATTAACAATCAAA
GAAAAAACGGAGTGAATGAG

Downstream 100 bases:

>100_bases
AACATTTATATAAAAGAAAAAGTAGCTATGAAATTTCATAGCTACTTTTTTTATTTTAAATTATAAGTAATTAAGTAATT
TATAAATATAAAATTATCCA

Product: nitrate extrusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKNLNLKGNPNRGLIGATLGFFIGFAAVSLYGPTSAVFKEAFVNLNPVLLALLIAAPNLSGSLLRIPFSAWVDTTGGRKP
LIVLLLLSIIGMGGLYIVLAFFSDNLNQYYYLLFALGLLSGCGIATFSVGVSQASYWFPKSKQGVALGIYGGVGNLAPGI
FALLIPNLALPLLGLPGSYLAWLIFLIVGTVIYIKIGQNAWYFQLVDKGINKDEAKEIASKEYGQELFPKGKASETLLIS
AKSWKTWALVFIYFTTFGGFLALTGWFPNYWMTYFGLNMKVAGLLTALYSILTSLTRIYGGKVADKNGGEITTIVSLGIA
LIGAICMTFASTMTLAIIGIILLAIGMGVANAAVFKIVPNAVPEAMGGASGWIGGLGALGGFLIPPVMASFLNRSGFAGY
SQGFSVFIVLIIVAIGVIALFQALQKKSAK

Sequences:

>Translated_430_residues
MKNLNLKGNPNRGLIGATLGFFIGFAAVSLYGPTSAVFKEAFVNLNPVLLALLIAAPNLSGSLLRIPFSAWVDTTGGRKP
LIVLLLLSIIGMGGLYIVLAFFSDNLNQYYYLLFALGLLSGCGIATFSVGVSQASYWFPKSKQGVALGIYGGVGNLAPGI
FALLIPNLALPLLGLPGSYLAWLIFLIVGTVIYIKIGQNAWYFQLVDKGINKDEAKEIASKEYGQELFPKGKASETLLIS
AKSWKTWALVFIYFTTFGGFLALTGWFPNYWMTYFGLNMKVAGLLTALYSILTSLTRIYGGKVADKNGGEITTIVSLGIA
LIGAICMTFASTMTLAIIGIILLAIGMGVANAAVFKIVPNAVPEAMGGASGWIGGLGALGGFLIPPVMASFLNRSGFAGY
SQGFSVFIVLIIVAIGVIALFQALQKKSAK
>Mature_430_residues
MKNLNLKGNPNRGLIGATLGFFIGFAAVSLYGPTSAVFKEAFVNLNPVLLALLIAAPNLSGSLLRIPFSAWVDTTGGRKP
LIVLLLLSIIGMGGLYIVLAFFSDNLNQYYYLLFALGLLSGCGIATFSVGVSQASYWFPKSKQGVALGIYGGVGNLAPGI
FALLIPNLALPLLGLPGSYLAWLIFLIVGTVIYIKIGQNAWYFQLVDKGINKDEAKEIASKEYGQELFPKGKASETLLIS
AKSWKTWALVFIYFTTFGGFLALTGWFPNYWMTYFGLNMKVAGLLTALYSILTSLTRIYGGKVADKNGGEITTIVSLGIA
LIGAICMTFASTMTLAIIGIILLAIGMGVANAAVFKIVPNAVPEAMGGASGWIGGLGALGGFLIPPVMASFLNRSGFAGY
SQGFSVFIVLIIVAIGVIALFQALQKKSAK

Specific function: Probably required for nitrate uptake under anoxic conditions. Also possibly involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45531; Mature: 45531

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLNLKGNPNRGLIGATLGFFIGFAAVSLYGPTSAVFKEAFVNLNPVLLALLIAAPNLS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCC
GSLLRIPFSAWVDTTGGRKPLIVLLLLSIIGMGGLYIVLAFFSDNLNQYYYLLFALGLLS
CCEEEECHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
GCGIATFSVGVSQASYWFPKSKQGVALGIYGGVGNLAPGIFALLIPNLALPLLGLPGSYL
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
AWLIFLIVGTVIYIKIGQNAWYFQLVDKGINKDEAKEIASKEYGQELFPKGKASETLLIS
HHHHHHHHHHHEEEEECCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
AKSWKTWALVFIYFTTFGGFLALTGWFPNYWMTYFGLNMKVAGLLTALYSILTSLTRIYG
ECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
GKVADKNGGEITTIVSLGIALIGAICMTFASTMTLAIIGIILLAIGMGVANAAVFKIVPN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCH
AVPEAMGGASGWIGGLGALGGFLIPPVMASFLNRSGFAGYSQGFSVFIVLIIVAIGVIAL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FQALQKKSAK
HHHHHHHCCC
>Mature Secondary Structure
MKNLNLKGNPNRGLIGATLGFFIGFAAVSLYGPTSAVFKEAFVNLNPVLLALLIAAPNLS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCC
GSLLRIPFSAWVDTTGGRKPLIVLLLLSIIGMGGLYIVLAFFSDNLNQYYYLLFALGLLS
CCEEEECHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHH
GCGIATFSVGVSQASYWFPKSKQGVALGIYGGVGNLAPGIFALLIPNLALPLLGLPGSYL
CCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
AWLIFLIVGTVIYIKIGQNAWYFQLVDKGINKDEAKEIASKEYGQELFPKGKASETLLIS
HHHHHHHHHHHEEEEECCCCEEHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
AKSWKTWALVFIYFTTFGGFLALTGWFPNYWMTYFGLNMKVAGLLTALYSILTSLTRIYG
ECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
GKVADKNGGEITTIVSLGIALIGAICMTFASTMTLAIIGIILLAIGMGVANAAVFKIVPN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCH
AVPEAMGGASGWIGGLGALGGFLIPPVMASFLNRSGFAGYSQGFSVFIVLIIVAIGVIAL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FQALQKKSAK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA