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The map label for this gene is apbE [H]

Identifier: 18311088

GI number: 18311088

Start: 2413977

End: 2415008

Strand: Reverse

Name: apbE [H]

Synonym: CPE2106

Alternate gene names: 18311088

Gene position: 2415008-2413977 (Counterclockwise)

Preceding gene: 18311089

Following gene: 18311087

Centisome position: 79.67

GC content: 29.26

Gene sequence:

>1032_bases
ATGTTGAAAAAGAGAGCAATTATTATTTTATTAGTTTCTATACTTTCTATGGGATTAATATCTTGTGATAATTCAAAAAA
AGTGGAAAACAATAATAAAGAAGTTAATTCCTATGAAAAAACAGAAAAGATTTTAGGTACTGTAGTTAGTGGAGTAGCAT
ATGGAGATAATGCTAAGGAAGCCTTAGAAAAAGCTTTTAATAGAGCAAAGGACATTGAGAATATGATGTCAGTAAATATA
GAGAATAGTGAGCTTAGCAAGGTGAATTCTGAAGCTTTTCATAAAAATGTTAAGTTATCAGATGATTTATATTATGTCAT
AGAAAAATCTATATATTATGCCAATTTAACTGATGGAGCTTTGGATCCAACTATTGGACATGTAATTGATTCTTGGGGAA
TAGGAACAGAACATGCTAATATACCTGAAAAAACTTTAATTGATAAGTATAAAGATTTAAAAAACTATAAGAATATAGAA
TTAAATCCTCATACTAAGGAAATAAGATTTTTAAATGAAAATATAAAATTAGATTTAGGAGCTATTGGCAAAGGATATGC
TGGAGATGAAATGAGAAAAGTTTTAAGGGAAGAGGGAATAAATTCAGCATTACTTAATTTAGGGGGAAATGTGGTTGCCT
TAGGAAATAAGATAAACAATGAAAATTGGAGCATAGGAATAAGAAATCCTAAGGAAGAGAATAAGATTTCAGCTTCTGTA
AAAATTAATGATGAAGTTGTGGTAACATCAGGAAATTATGAAAGATATTTTATAAAAGATGGAGTTAGATATCATCATAT
ATTAGATCCTAGCACTGCTTATCCAGCTGAAAAGGGACTTATAAGTTCAACAATAATCACTAAAAATGGAATAGATGCAG
ATGCCTTATCAACAGCTACTTATATTTTAGGGGCAGAAAAAGCTAAGAAACTTATTGAAGGATTAGATGGGGTAGAGGCA
TTATTTATAAAAGATAATATGGACTTCATAGAAACAAGCAACTTGGATAACAAAGGATTTAGGGGGATGTAA

Upstream 100 bases:

>100_bases
TACTTAATTAGATTTAAAAGATGATTTTGCTTAATCAAAGTCATCTTTTAATCTAAATGTGTATTTAAATTTTAAGAGAT
ATTTATTGGAGTCAAAATAT

Downstream 100 bases:

>100_bases
ATGAATAAAAAAGTTCAAGTGGTAATAATTTTTACGATTTTATTGTTATCTACTTTGTTATGTTTTTTTATACATAATAA
AAAAGTTAAAAATCCAGTAG

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLKKRAIIILLVSILSMGLISCDNSKKVENNNKEVNSYEKTEKILGTVVSGVAYGDNAKEALEKAFNRAKDIENMMSVNI
ENSELSKVNSEAFHKNVKLSDDLYYVIEKSIYYANLTDGALDPTIGHVIDSWGIGTEHANIPEKTLIDKYKDLKNYKNIE
LNPHTKEIRFLNENIKLDLGAIGKGYAGDEMRKVLREEGINSALLNLGGNVVALGNKINNENWSIGIRNPKEENKISASV
KINDEVVVTSGNYERYFIKDGVRYHHILDPSTAYPAEKGLISSTIITKNGIDADALSTATYILGAEKAKKLIEGLDGVEA
LFIKDNMDFIETSNLDNKGFRGM

Sequences:

>Translated_343_residues
MLKKRAIIILLVSILSMGLISCDNSKKVENNNKEVNSYEKTEKILGTVVSGVAYGDNAKEALEKAFNRAKDIENMMSVNI
ENSELSKVNSEAFHKNVKLSDDLYYVIEKSIYYANLTDGALDPTIGHVIDSWGIGTEHANIPEKTLIDKYKDLKNYKNIE
LNPHTKEIRFLNENIKLDLGAIGKGYAGDEMRKVLREEGINSALLNLGGNVVALGNKINNENWSIGIRNPKEENKISASV
KINDEVVVTSGNYERYFIKDGVRYHHILDPSTAYPAEKGLISSTIITKNGIDADALSTATYILGAEKAKKLIEGLDGVEA
LFIKDNMDFIETSNLDNKGFRGM
>Mature_343_residues
MLKKRAIIILLVSILSMGLISCDNSKKVENNNKEVNSYEKTEKILGTVVSGVAYGDNAKEALEKAFNRAKDIENMMSVNI
ENSELSKVNSEAFHKNVKLSDDLYYVIEKSIYYANLTDGALDPTIGHVIDSWGIGTEHANIPEKTLIDKYKDLKNYKNIE
LNPHTKEIRFLNENIKLDLGAIGKGYAGDEMRKVLREEGINSALLNLGGNVVALGNKINNENWSIGIRNPKEENKISASV
KINDEVVVTSGNYERYFIKDGVRYHHILDPSTAYPAEKGLISSTIITKNGIDADALSTATYILGAEKAKKLIEGLDGVEA
LFIKDNMDFIETSNLDNKGFRGM

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=317, Percent_Identity=29.6529968454259, Blast_Score=144, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38053; Mature: 38053

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKRAIIILLVSILSMGLISCDNSKKVENNNKEVNSYEKTEKILGTVVSGVAYGDNAKE
CCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALEKAFNRAKDIENMMSVNIENSELSKVNSEAFHKNVKLSDDLYYVIEKSIYYANLTDGA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCC
LDPTIGHVIDSWGIGTEHANIPEKTLIDKYKDLKNYKNIELNPHTKEIRFLNENIKLDLG
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCEEEEC
AIGKGYAGDEMRKVLREEGINSALLNLGGNVVALGNKINNENWSIGIRNPKEENKISASV
CCCCCCCCHHHHHHHHHCCCCHHHHHCCCCEEEECCEECCCCEEEEECCCCCCCCEEEEE
KINDEVVVTSGNYERYFIKDGVRYHHILDPSTAYPAEKGLISSTIITKNGIDADALSTAT
EECCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
YILGAEKAKKLIEGLDGVEALFIKDNMDFIETSNLDNKGFRGM
HHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MLKKRAIIILLVSILSMGLISCDNSKKVENNNKEVNSYEKTEKILGTVVSGVAYGDNAKE
CCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
ALEKAFNRAKDIENMMSVNIENSELSKVNSEAFHKNVKLSDDLYYVIEKSIYYANLTDGA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCC
LDPTIGHVIDSWGIGTEHANIPEKTLIDKYKDLKNYKNIELNPHTKEIRFLNENIKLDLG
CCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHEEEEECCCCEEEEC
AIGKGYAGDEMRKVLREEGINSALLNLGGNVVALGNKINNENWSIGIRNPKEENKISASV
CCCCCCCCHHHHHHHHHCCCCHHHHHCCCCEEEECCEECCCCEEEEECCCCCCCCEEEEE
KINDEVVVTSGNYERYFIKDGVRYHHILDPSTAYPAEKGLISSTIITKNGIDADALSTAT
EECCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
YILGAEKAKKLIEGLDGVEALFIKDNMDFIETSNLDNKGFRGM
HHHHHHHHHHHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]