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The map label for this gene is podK [H]

Identifier: 18310993

GI number: 18310993

Start: 2303526

End: 2306156

Strand: Reverse

Name: podK [H]

Synonym: CPE2011

Alternate gene names: 18310993

Gene position: 2306156-2303526 (Counterclockwise)

Preceding gene: 18310994

Following gene: 18310991

Centisome position: 76.07

GC content: 33.75

Gene sequence:

>2631_bases
ATGGAAAAAAAGCAGTATGTTTATCTTTTTAATGAAGGTAACGCAAGTATGAAAAATTTATTAGGTGGAAAAGGTGCTAA
CTTATCCGAGATGACTATTTTAGGAATACCAGTTCCACAAGGATTTACAGTAACAACTGAAGCATGTAATAAGTATTATG
AAGATGACAAAAAGATTTCTCAAGATATTATTGAAGAGATTGAAAATAAAATGTCTGAGTTAGAAAAAATAACTGGCAAA
AAATTTGGTTCTCTTGAAAATCCATTATTAGTATCAGTTAGATCTGGTGCAAGAGTTTCAATGCCGGGTATGATGGATAC
AATTCTAAACCTTGGATTAAATGATGAATCAGTAGAAGCTATGGCTAAATTAACAAATAATCCAAGATTTGCTTATGATT
CATATAGAAGATTTATTCAAATGTTTGCTGATGTTGTAATGGGTGTTGAAAAAAGACTTTTTGAAGATTTACTAGACGAA
GTTAAGGAAGAAAAAGGTTATAAAATAGATACTGATTTAACAGCAGAGGATTTAAAAGATTTAGTAGTAAAATTTAAAGC
GCTTTATAAAAAGGAAAAGGGAGAAGATTTTCCAAGTAATCCAAAAGAACAACTTATAGAGGCAGTTACAGCTGTATTTA
GATCATGGAATAATCCAAGAGCAATTGTTTATAGAAGATTAAATGATATTCCAGGAGAATGGGGTACAGCAGTTAACGTT
CAAGAGATGGTATTTGGAAATAAGGGAGAAACTTCAGGAACTGGAGTTGCATTTTCAAGAAACCCAGCTAATGGGGATAA
TGAATTATATGGTGAGTATTTAATGAATGCTCAAGGTGAGGATGTTGTTGCAGGTATAAGAACACCAGAGCCAATAAGCC
ATTTAGAAGCTCAAAACCCTACAATATATAAGCAATTTACAGATATAGTTAATACATTAGAAAAGCATTATAGAGATATG
CAAGACATGGAATTTACAATAGAAGATGGAAAGTTATATTTCCTTCAAACTAGAAATGGTAAGAGAACTGCTCAAGCAGC
TTTAAAAATAGCTGTTGATTTAGTTGAAGAAGGAATGTTAACTAAGGAAGAGGCTATATTAAAAGTTGAGCCTAAACAAT
TAGATACATTACTTCACCCTGCTTTTGCATCTGATGGCTTAAAAGAAGCTAAAATAGTTGCAAAGGGATTACCAGCATCA
CCAGGAGCTGCATGTGGTAAAATAGCTTTCACAGCTGAAGAAGCTAAGGAAAGAAAAGCAAATGGTGAAAAGGTTGTATT
AGTAAGATTAGAGACTTCACCAGAAGATATAGAAGGTATGATTGCTGCAGAAGGTATCTTAACTGTTAGAGGAGGAATGA
CTTCTCATGCAGCCGTTGTAGCAAGAGGAATGGGTACTTGCTGTGTAGCAGGATGTGGAGAATTAAAAGTTAATGAAGAA
GCTAGAACTTTAGAAGTTAATGGACAAGTATTAACCTTTGATGATTATATCTCAATAGATGGTTCAACAGGACACATCTA
TGCTGAACAAGTTAAGACAGTATCTCCAGAAATTACAGGACATTTCGCTACATTTATGGGATGGGCTGATGAAATAAGAA
AGTTAAAGGTTAGAACTAATGCTGATACACCAAGAGATACTAAACAAGCTGTTGAATTTGGTGCAGAAGGTATTGGATTA
TGTAGAACAGAGCATATGTTCTTTGCTGAAGATAGAATTTTAGCTGTTAGAGAAATGATTTTAGCTAAGAATGAGGATAG
TAGAAGAGTTGCATTAGAAAAACTTCTACCAATGCAAAGAGAAGACTTTATAGGAATATATGAAGCTTTAGAAGAAAGAC
CAGCTACAATAAGATTCTTAGATCCACCTCTACATGAATTCTTACCAAGTGAAGAGGAAGATATAAATGCCTTAGCTAAA
GAAATTGGAGTAAGTCCAGCTGAGATTAAGAATGTAGTTGCTGAATTACATGAATTTAACCCAATGATGGGTCATAGAGG
ATGTCGTTTAGCAGTATCTTATCCAGAGATAGCTGAAATGCAAACAAGAGCTGTCATAGAGGCTGCCATAGAAGTTAAAA
AGAATAAAGGATACAATATAGTTCCTGAAATAATGATTCCTTTAATAGGTGAAATAAAAGAATTAAAATATGTTAAAAAT
GTAGTTGTTGAAACAGCTAAGACAGTAATGGAAGAGAAAGGTGTTCAATTAGATTATAAGGTTGGAACAATGATTGAAAT
TCCAAGAGCTGCTTTAACAGCTGATAAGATAGCAGAAGAGGCTGAGTTCTTCTCCTTTGGAACAAATGACTTGACTCAAA
TGACCTTTGGATTCTCAAGAGATGATGCAGCTAAATTCTTAAAAGATTACTATGAAAAAGGAATATATGAGCAAGATCCA
TTTGCTAAATTAGACCAAGAAGGCGTTGGAGAGCTTATGAGAATTGCTTGTGAAAAAGGTAAGGCAACAAGACCTGATAT
TAAATTAGGTATATGTGGAGAACACGGTGGAGATCCTTCATCTGTTGAATTCTGCCACAACTTAGGATTAAATTACGTAA
GTTGTTCACCTTATAGAGTTCCATTAGCAAGACTTGCAGCTGCACAAGCACAAGTTAAAAATAAAAGATAA

Upstream 100 bases:

>100_bases
ATTTATGGTTAAAAAGAAAGATTAAATGGGTAACTATCGGGGGTAATACTCTGTTAGTATATAAAAATTCAACTATAGTA
TTGAACGGAGGAGTATTGAG

Downstream 100 bases:

>100_bases
TAAATAAGATTATATAAAATAAAGGAGTATTCATTATGAATACTCCTTTATTTAAACTTTAATTTTAAATTCAGTAGAAA
ACAATGTTCTAAGTTCTCCA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 876; Mature: 876

Protein sequence:

>876_residues
MEKKQYVYLFNEGNASMKNLLGGKGANLSEMTILGIPVPQGFTVTTEACNKYYEDDKKISQDIIEEIENKMSELEKITGK
KFGSLENPLLVSVRSGARVSMPGMMDTILNLGLNDESVEAMAKLTNNPRFAYDSYRRFIQMFADVVMGVEKRLFEDLLDE
VKEEKGYKIDTDLTAEDLKDLVVKFKALYKKEKGEDFPSNPKEQLIEAVTAVFRSWNNPRAIVYRRLNDIPGEWGTAVNV
QEMVFGNKGETSGTGVAFSRNPANGDNELYGEYLMNAQGEDVVAGIRTPEPISHLEAQNPTIYKQFTDIVNTLEKHYRDM
QDMEFTIEDGKLYFLQTRNGKRTAQAALKIAVDLVEEGMLTKEEAILKVEPKQLDTLLHPAFASDGLKEAKIVAKGLPAS
PGAACGKIAFTAEEAKERKANGEKVVLVRLETSPEDIEGMIAAEGILTVRGGMTSHAAVVARGMGTCCVAGCGELKVNEE
ARTLEVNGQVLTFDDYISIDGSTGHIYAEQVKTVSPEITGHFATFMGWADEIRKLKVRTNADTPRDTKQAVEFGAEGIGL
CRTEHMFFAEDRILAVREMILAKNEDSRRVALEKLLPMQREDFIGIYEALEERPATIRFLDPPLHEFLPSEEEDINALAK
EIGVSPAEIKNVVAELHEFNPMMGHRGCRLAVSYPEIAEMQTRAVIEAAIEVKKNKGYNIVPEIMIPLIGEIKELKYVKN
VVVETAKTVMEEKGVQLDYKVGTMIEIPRAALTADKIAEEAEFFSFGTNDLTQMTFGFSRDDAAKFLKDYYEKGIYEQDP
FAKLDQEGVGELMRIACEKGKATRPDIKLGICGEHGGDPSSVEFCHNLGLNYVSCSPYRVPLARLAAAQAQVKNKR

Sequences:

>Translated_876_residues
MEKKQYVYLFNEGNASMKNLLGGKGANLSEMTILGIPVPQGFTVTTEACNKYYEDDKKISQDIIEEIENKMSELEKITGK
KFGSLENPLLVSVRSGARVSMPGMMDTILNLGLNDESVEAMAKLTNNPRFAYDSYRRFIQMFADVVMGVEKRLFEDLLDE
VKEEKGYKIDTDLTAEDLKDLVVKFKALYKKEKGEDFPSNPKEQLIEAVTAVFRSWNNPRAIVYRRLNDIPGEWGTAVNV
QEMVFGNKGETSGTGVAFSRNPANGDNELYGEYLMNAQGEDVVAGIRTPEPISHLEAQNPTIYKQFTDIVNTLEKHYRDM
QDMEFTIEDGKLYFLQTRNGKRTAQAALKIAVDLVEEGMLTKEEAILKVEPKQLDTLLHPAFASDGLKEAKIVAKGLPAS
PGAACGKIAFTAEEAKERKANGEKVVLVRLETSPEDIEGMIAAEGILTVRGGMTSHAAVVARGMGTCCVAGCGELKVNEE
ARTLEVNGQVLTFDDYISIDGSTGHIYAEQVKTVSPEITGHFATFMGWADEIRKLKVRTNADTPRDTKQAVEFGAEGIGL
CRTEHMFFAEDRILAVREMILAKNEDSRRVALEKLLPMQREDFIGIYEALEERPATIRFLDPPLHEFLPSEEEDINALAK
EIGVSPAEIKNVVAELHEFNPMMGHRGCRLAVSYPEIAEMQTRAVIEAAIEVKKNKGYNIVPEIMIPLIGEIKELKYVKN
VVVETAKTVMEEKGVQLDYKVGTMIEIPRAALTADKIAEEAEFFSFGTNDLTQMTFGFSRDDAAKFLKDYYEKGIYEQDP
FAKLDQEGVGELMRIACEKGKATRPDIKLGICGEHGGDPSSVEFCHNLGLNYVSCSPYRVPLARLAAAQAQVKNKR
>Mature_876_residues
MEKKQYVYLFNEGNASMKNLLGGKGANLSEMTILGIPVPQGFTVTTEACNKYYEDDKKISQDIIEEIENKMSELEKITGK
KFGSLENPLLVSVRSGARVSMPGMMDTILNLGLNDESVEAMAKLTNNPRFAYDSYRRFIQMFADVVMGVEKRLFEDLLDE
VKEEKGYKIDTDLTAEDLKDLVVKFKALYKKEKGEDFPSNPKEQLIEAVTAVFRSWNNPRAIVYRRLNDIPGEWGTAVNV
QEMVFGNKGETSGTGVAFSRNPANGDNELYGEYLMNAQGEDVVAGIRTPEPISHLEAQNPTIYKQFTDIVNTLEKHYRDM
QDMEFTIEDGKLYFLQTRNGKRTAQAALKIAVDLVEEGMLTKEEAILKVEPKQLDTLLHPAFASDGLKEAKIVAKGLPAS
PGAACGKIAFTAEEAKERKANGEKVVLVRLETSPEDIEGMIAAEGILTVRGGMTSHAAVVARGMGTCCVAGCGELKVNEE
ARTLEVNGQVLTFDDYISIDGSTGHIYAEQVKTVSPEITGHFATFMGWADEIRKLKVRTNADTPRDTKQAVEFGAEGIGL
CRTEHMFFAEDRILAVREMILAKNEDSRRVALEKLLPMQREDFIGIYEALEERPATIRFLDPPLHEFLPSEEEDINALAK
EIGVSPAEIKNVVAELHEFNPMMGHRGCRLAVSYPEIAEMQTRAVIEAAIEVKKNKGYNIVPEIMIPLIGEIKELKYVKN
VVVETAKTVMEEKGVQLDYKVGTMIEIPRAALTADKIAEEAEFFSFGTNDLTQMTFGFSRDDAAKFLKDYYEKGIYEQDP
FAKLDQEGVGELMRIACEKGKATRPDIKLGICGEHGGDPSSVEFCHNLGLNYVSCSPYRVPLARLAAAQAQVKNKR

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=908, Percent_Identity=24.1189427312775, Blast_Score=117, Evalue=4e-27,
Organism=Escherichia coli, GI1788756, Length=465, Percent_Identity=24.7311827956989, Blast_Score=113, Evalue=6e-26,
Organism=Escherichia coli, GI48994992, Length=452, Percent_Identity=25.2212389380531, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789193, Length=418, Percent_Identity=23.6842105263158, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788726, Length=440, Percent_Identity=23.4090909090909, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97414; Mature: 97414

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKQYVYLFNEGNASMKNLLGGKGANLSEMTILGIPVPQGFTVTTEACNKYYEDDKKIS
CCCCEEEEEEECCCHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHH
QDIIEEIENKMSELEKITGKKFGSLENPLLVSVRSGARVSMPGMMDTILNLGLNDESVEA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHH
MAKLTNNPRFAYDSYRRFIQMFADVVMGVEKRLFEDLLDEVKEEKGYKIDTDLTAEDLKD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHH
LVVKFKALYKKEKGEDFPSNPKEQLIEAVTAVFRSWNNPRAIVYRRLNDIPGEWGTAVNV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCH
QEMVFGNKGETSGTGVAFSRNPANGDNELYGEYLMNAQGEDVVAGIRTPEPISHLEAQNP
HHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCC
TIYKQFTDIVNTLEKHYRDMQDMEFTIEDGKLYFLQTRNGKRTAQAALKIAVDLVEEGML
HHHHHHHHHHHHHHHHHCCHHCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
TKEEAILKVEPKQLDTLLHPAFASDGLKEAKIVAKGLPASPGAACGKIAFTAEEAKERKA
CCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
NGEKVVLVRLETSPEDIEGMIAAEGILTVRGGMTSHAAVVARGMGTCCVAGCGELKVNEE
CCCEEEEEEECCCCHHHHHHEEECCEEEECCCCCHHHHHHHHCCCHHHHCCCCCEEECCC
ARTLEVNGQVLTFDDYISIDGSTGHIYAEQVKTVSPEITGHFATFMGWADEIRKLKVRTN
CEEEEECCEEEEEECEEEECCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECC
ADTPRDTKQAVEFGAEGIGLCRTEHMFFAEDRILAVREMILAKNEDSRRVALEKLLPMQR
CCCCCHHHHHHHHCCCCCCEEEECCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
EDFIGIYEALEERPATIRFLDPPLHEFLPSEEEDINALAKEIGVSPAEIKNVVAELHEFN
HHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PMMGHRGCRLAVSYPEIAEMQTRAVIEAAIEVKKNKGYNIVPEIMIPLIGEIKELKYVKN
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVVETAKTVMEEKGVQLDYKVGTMIEIPRAALTADKIAEEAEFFSFGTNDLTQMTFGFSR
HHHHHHHHHHHHCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
DDAAKFLKDYYEKGIYEQDPFAKLDQEGVGELMRIACEKGKATRPDIKLGICGEHGGDPS
HHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCH
SVEFCHNLGLNYVSCSPYRVPLARLAAAQAQVKNKR
HHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKKQYVYLFNEGNASMKNLLGGKGANLSEMTILGIPVPQGFTVTTEACNKYYEDDKKIS
CCCCEEEEEEECCCHHHHHHCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHH
QDIIEEIENKMSELEKITGKKFGSLENPLLVSVRSGARVSMPGMMDTILNLGLNDESVEA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHH
MAKLTNNPRFAYDSYRRFIQMFADVVMGVEKRLFEDLLDEVKEEKGYKIDTDLTAEDLKD
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHH
LVVKFKALYKKEKGEDFPSNPKEQLIEAVTAVFRSWNNPRAIVYRRLNDIPGEWGTAVNV
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCH
QEMVFGNKGETSGTGVAFSRNPANGDNELYGEYLMNAQGEDVVAGIRTPEPISHLEAQNP
HHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCC
TIYKQFTDIVNTLEKHYRDMQDMEFTIEDGKLYFLQTRNGKRTAQAALKIAVDLVEEGML
HHHHHHHHHHHHHHHHHCCHHCCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCC
TKEEAILKVEPKQLDTLLHPAFASDGLKEAKIVAKGLPASPGAACGKIAFTAEEAKERKA
CCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHC
NGEKVVLVRLETSPEDIEGMIAAEGILTVRGGMTSHAAVVARGMGTCCVAGCGELKVNEE
CCCEEEEEEECCCCHHHHHHEEECCEEEECCCCCHHHHHHHHCCCHHHHCCCCCEEECCC
ARTLEVNGQVLTFDDYISIDGSTGHIYAEQVKTVSPEITGHFATFMGWADEIRKLKVRTN
CEEEEECCEEEEEECEEEECCCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECC
ADTPRDTKQAVEFGAEGIGLCRTEHMFFAEDRILAVREMILAKNEDSRRVALEKLLPMQR
CCCCCHHHHHHHHCCCCCCEEEECCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCH
EDFIGIYEALEERPATIRFLDPPLHEFLPSEEEDINALAKEIGVSPAEIKNVVAELHEFN
HHHHHHHHHHHCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PMMGHRGCRLAVSYPEIAEMQTRAVIEAAIEVKKNKGYNIVPEIMIPLIGEIKELKYVKN
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VVVETAKTVMEEKGVQLDYKVGTMIEIPRAALTADKIAEEAEFFSFGTNDLTQMTFGFSR
HHHHHHHHHHHHCCCEEEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
DDAAKFLKDYYEKGIYEQDPFAKLDQEGVGELMRIACEKGKATRPDIKLGICGEHGGDPS
HHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCH
SVEFCHNLGLNYVSCSPYRVPLARLAAAQAQVKNKR
HHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]