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The map label for this gene is rihA [H]

Identifier: 18310985

GI number: 18310985

Start: 2293974

End: 2294954

Strand: Reverse

Name: rihA [H]

Synonym: CPE2003

Alternate gene names: 18310985

Gene position: 2294954-2293974 (Counterclockwise)

Preceding gene: 18310986

Following gene: 18310984

Centisome position: 75.71

GC content: 32.01

Gene sequence:

>981_bases
ATGGATAAGAGAAAGGTAATTATTGATTGTGATCCAGGGATAGACGATGCTTTAGCCATTATTCTTGCATTAAAGTCAAA
AGAGATTGAGGTTGTTGGAATAACCACCGTATCAGGAAATGTTGAAAGTTTACAGGGAGCTAAAAATGCCTTAAAGGTAC
TTAAGCTTTTAGGTAGATTGGATATTCCTGTTTACTTAGGAGAAGGTAAGCCAGTTAAAAGAGAGCTTGTAACAGCACAG
GATACTCATGGGGAAGATGGCTTAGGAGAAACTTTTTTAGAAGAGGTCTCTAGTGAGTATATTAGAGAAAATGGTGTTGA
TTTTATTTTAAATACTTTAAAAAATCATGAAAATGTTAGTATTATTGCTCTTGGACCCCTAACAAACCTATGTAGAGCTA
TAGAAAAGGATTCAGAAAGTTTTCATAGAGTTAAGGAAATAGTTTCTATGGGTGGAGCTTATAAGAGTCATGGAAATTGT
TCACCAGTAGCTGAATTTAATTACTGGGTAGATCCTCATGGAGCAAGGGAGTTTCTAAAAAAGTTTAATGGTGAATTTAC
CATGGTTGGTTTAGATGTTACAAGAGAGATAGTTTTAACACCAAATTTAAGAGAAATGATACATCAATTTAAAGATGAAA
TTGGTGATTTTATATATGATATTACTAGATTCTACGTTGATTTCCATTGGGAACAAGAGAGAACACTTGGATGTGTTATA
AATGACCCCTTAGCAGTAGAGTATTTTATAAATAGAGAGCTTTGTGAGGGTTTTAAGGCTTATGTGGACATAGCTTGCGA
AGATATATCAATGGGACAAAGTGTTGTTGATGTTGCAGATTTTTATAAGAAAAGAAAAAATGTATTTGTTTTAGATAAAG
TTAATAGCAAAGAATTTATGGTAAGTTTTCTTAATAAAATATTTCCGATCCATAAAGAGGATATTAAAAATGTACTTAAT
AATCCAAAGTATGGTATTTAA

Upstream 100 bases:

>100_bases
TTAGTTTTAAAATTTATCTTAGTAAAAAGATAGTTATAAGGAAGCTTAAAGATAATAACAGTTCCTTTTGGTGAAGTTAA
ATCAAAGGGAGGAAATTAAA

Downstream 100 bases:

>100_bases
TAGGGAGGGAATATTAATATGAGAAATTCAAAAAGTTTAAAGTTAACAATAATGGGAATAGGAATAGCATTAAATATAAT
TGGAGCTTTTATAGCCTTAA

Product: nucleoside hydrolase, IUNH family

Products: cytosine; ribose; uracil [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MDKRKVIIDCDPGIDDALAIILALKSKEIEVVGITTVSGNVESLQGAKNALKVLKLLGRLDIPVYLGEGKPVKRELVTAQ
DTHGEDGLGETFLEEVSSEYIRENGVDFILNTLKNHENVSIIALGPLTNLCRAIEKDSESFHRVKEIVSMGGAYKSHGNC
SPVAEFNYWVDPHGAREFLKKFNGEFTMVGLDVTREIVLTPNLREMIHQFKDEIGDFIYDITRFYVDFHWEQERTLGCVI
NDPLAVEYFINRELCEGFKAYVDIACEDISMGQSVVDVADFYKKRKNVFVLDKVNSKEFMVSFLNKIFPIHKEDIKNVLN
NPKYGI

Sequences:

>Translated_326_residues
MDKRKVIIDCDPGIDDALAIILALKSKEIEVVGITTVSGNVESLQGAKNALKVLKLLGRLDIPVYLGEGKPVKRELVTAQ
DTHGEDGLGETFLEEVSSEYIRENGVDFILNTLKNHENVSIIALGPLTNLCRAIEKDSESFHRVKEIVSMGGAYKSHGNC
SPVAEFNYWVDPHGAREFLKKFNGEFTMVGLDVTREIVLTPNLREMIHQFKDEIGDFIYDITRFYVDFHWEQERTLGCVI
NDPLAVEYFINRELCEGFKAYVDIACEDISMGQSVVDVADFYKKRKNVFVLDKVNSKEFMVSFLNKIFPIHKEDIKNVLN
NPKYGI
>Mature_326_residues
MDKRKVIIDCDPGIDDALAIILALKSKEIEVVGITTVSGNVESLQGAKNALKVLKLLGRLDIPVYLGEGKPVKRELVTAQ
DTHGEDGLGETFLEEVSSEYIRENGVDFILNTLKNHENVSIIALGPLTNLCRAIEKDSESFHRVKEIVSMGGAYKSHGNC
SPVAEFNYWVDPHGAREFLKKFNGEFTMVGLDVTREIVLTPNLREMIHQFKDEIGDFIYDITRFYVDFHWEQERTLGCVI
NDPLAVEYFINRELCEGFKAYVDIACEDISMGQSVVDVADFYKKRKNVFVLDKVNSKEFMVSFLNKIFPIHKEDIKNVLN
NPKYGI

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=301, Percent_Identity=31.8936877076412, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1786871, Length=299, Percent_Identity=32.1070234113712, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=29.3729372937294, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32565474, Length=199, Percent_Identity=34.6733668341709, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17565698, Length=198, Percent_Identity=30.8080808080808, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320608, Length=285, Percent_Identity=30.5263157894737, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583915, Length=195, Percent_Identity=32.8205128205128, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641839, Length=198, Percent_Identity=34.3434343434343, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24641837, Length=197, Percent_Identity=32.48730964467, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 36904; Mature: 36904

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKRKVIIDCDPGIDDALAIILALKSKEIEVVGITTVSGNVESLQGAKNALKVLKLLGRL
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCC
DIPVYLGEGKPVKRELVTAQDTHGEDGLGETFLEEVSSEYIRENGVDFILNTLKNHENVS
CCEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
IIALGPLTNLCRAIEKDSESFHRVKEIVSMGGAYKSHGNCSPVAEFNYWVDPHGAREFLK
EEEECCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCHHHHHHH
KFNGEFTMVGLDVTREIVLTPNLREMIHQFKDEIGDFIYDITRFYVDFHWEQERTLGCVI
HHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEE
NDPLAVEYFINRELCEGFKAYVDIACEDISMGQSVVDVADFYKKRKNVFVLDKVNSKEFM
CCCEEEHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCHHHH
VSFLNKIFPIHKEDIKNVLNNPKYGI
HHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDKRKVIIDCDPGIDDALAIILALKSKEIEVVGITTVSGNVESLQGAKNALKVLKLLGRL
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHCCC
DIPVYLGEGKPVKRELVTAQDTHGEDGLGETFLEEVSSEYIRENGVDFILNTLKNHENVS
CCEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEE
IIALGPLTNLCRAIEKDSESFHRVKEIVSMGGAYKSHGNCSPVAEFNYWVDPHGAREFLK
EEEECCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCHHHHHHH
KFNGEFTMVGLDVTREIVLTPNLREMIHQFKDEIGDFIYDITRFYVDFHWEQERTLGCVI
HHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEE
NDPLAVEYFINRELCEGFKAYVDIACEDISMGQSVVDVADFYKKRKNVFVLDKVNSKEFM
CCCEEEHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCHHHH
VSFLNKIFPIHKEDIKNVLNNPKYGI
HHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA