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The map label for this gene is dacF [H]

Identifier: 18310958

GI number: 18310958

Start: 2263061

End: 2264332

Strand: Direct

Name: dacF [H]

Synonym: CPE1976

Alternate gene names: 18310958

Gene position: 2263061-2264332 (Clockwise)

Preceding gene: 18310955

Following gene: 18310960

Centisome position: 74.65

GC content: 30.97

Gene sequence:

>1272_bases
TTGATTAAATTTAAGAAAAAGCTTTTAAGTTCAATATTGATTGCTTTATCAGTATCACTTTTTACGCCAACAAAAGCTAC
TGTTCAGGCTGCCGAAATTTCCCAACCTAATATAGTTGGAAAGTATGCTGTCACTTTAGATTATGACACTGGTGAAATTA
TTTATGCAAAGGGAATTGATGAAAAAGCATATCCTGCTAGTACAACAAAGGTAATGACAAGTCTTTTATTTGCTGAACAT
GCTTCAAAGAATGATTCTTTTCCATATACAGCAGATGCAAAAGCTCAACAACCTTATACATTAAACAATAGCTTTGGACC
AATACCTGTCGGCGAAGGAATGAATGCTAATGATTTAATGAAAGCTTTACTTATGTTTTCAGCTAATGATGCTGCCGCTG
TAATTGCAGACGGGGTGGCTGGAAGTGCTGAAAAATTTAGTGTAATGATGAATGACGAAGTAAAAAAATTAGGATTAAAA
AATACTCACTTTGTTACTCCAAATGGCTTACATAATGATGATCACTATTCAACAGCTTACGATTTAGCTGTTATTTTACA
AAATGCTTATAAAAATCCTTGGGTAAGCGAAACTATGGCACTTAAGGATAGCGACATAACTGTAAACGGAAAAAAAGTAC
TTTTAGAAAATAGAAATAAAGAACTTGGTATCGACGGAAATATTGGTGGAAAGACTGGATTTACAACTCCTGCTGGAAGA
TGTTTAGTATCAGTATACGAAAGAAATGGTAGAAAAATAATAGGTGCTGTTTTAAATTCTCAATATGATGCTAAGGATGA
AATTGTATTTAATGATATGAATAAAATTATTGATTACAGTTACTCTGTAGATAAAGTTCCATACATAAAGGCTGGTACAA
CAATAGATACTATTCCAGTTGAATATAAACTTTTTAGATGGTTTGGACCAACTAAGAAAATCGACGTTCCTTTTGTTGCA
ACTGAAAACATAGATTATTATAAAAATTATGTAAATGAAAAAGAAACATCAAAATCAATAAACTTAAATGATATGAATGC
TTGGCAATTAGCTTCTAATCCTGAATCAGCTGCTGTTACTGTTACTCAAAGAGCTTACGTTAAGGATTATCCAGTAAAAG
CTGATATAGGTACCTTTACTCTTATAAAAGCTAATTTCTTAAGTTATTTAGGAATAATTGTTCTAGTAGTTGTTGCGATT
GTATTAATATTACTTATTATAAGAGCAATAAATTTAAGAAAACGTAGAAGACGTAGAAGAAATATATTTTAA

Upstream 100 bases:

>100_bases
AAAGCTTTACATCTCTTTCTTTATTATTATTGTTATTATGCCCTATAGATGTTATAATAAAAAAAGTTTAATGAGACTTT
GAAATTAGGAGAGATGAAAA

Downstream 100 bases:

>100_bases
TAAAAAAAAGATTTAGTCTTAGACTAAATCTTTTTTTATTAAAAACCTATTAAATTAAGAATTTAATTTTTCAACAAGAT
CTAAAATTTCTAAAGGTTTA

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MIKFKKKLLSSILIALSVSLFTPTKATVQAAEISQPNIVGKYAVTLDYDTGEIIYAKGIDEKAYPASTTKVMTSLLFAEH
ASKNDSFPYTADAKAQQPYTLNNSFGPIPVGEGMNANDLMKALLMFSANDAAAVIADGVAGSAEKFSVMMNDEVKKLGLK
NTHFVTPNGLHNDDHYSTAYDLAVILQNAYKNPWVSETMALKDSDITVNGKKVLLENRNKELGIDGNIGGKTGFTTPAGR
CLVSVYERNGRKIIGAVLNSQYDAKDEIVFNDMNKIIDYSYSVDKVPYIKAGTTIDTIPVEYKLFRWFGPTKKIDVPFVA
TENIDYYKNYVNEKETSKSINLNDMNAWQLASNPESAAVTVTQRAYVKDYPVKADIGTFTLIKANFLSYLGIIVLVVVAI
VLILLIIRAINLRKRRRRRRNIF

Sequences:

>Translated_423_residues
MIKFKKKLLSSILIALSVSLFTPTKATVQAAEISQPNIVGKYAVTLDYDTGEIIYAKGIDEKAYPASTTKVMTSLLFAEH
ASKNDSFPYTADAKAQQPYTLNNSFGPIPVGEGMNANDLMKALLMFSANDAAAVIADGVAGSAEKFSVMMNDEVKKLGLK
NTHFVTPNGLHNDDHYSTAYDLAVILQNAYKNPWVSETMALKDSDITVNGKKVLLENRNKELGIDGNIGGKTGFTTPAGR
CLVSVYERNGRKIIGAVLNSQYDAKDEIVFNDMNKIIDYSYSVDKVPYIKAGTTIDTIPVEYKLFRWFGPTKKIDVPFVA
TENIDYYKNYVNEKETSKSINLNDMNAWQLASNPESAAVTVTQRAYVKDYPVKADIGTFTLIKANFLSYLGIIVLVVVAI
VLILLIIRAINLRKRRRRRRNIF
>Mature_423_residues
MIKFKKKLLSSILIALSVSLFTPTKATVQAAEISQPNIVGKYAVTLDYDTGEIIYAKGIDEKAYPASTTKVMTSLLFAEH
ASKNDSFPYTADAKAQQPYTLNNSFGPIPVGEGMNANDLMKALLMFSANDAAAVIADGVAGSAEKFSVMMNDEVKKLGLK
NTHFVTPNGLHNDDHYSTAYDLAVILQNAYKNPWVSETMALKDSDITVNGKKVLLENRNKELGIDGNIGGKTGFTTPAGR
CLVSVYERNGRKIIGAVLNSQYDAKDEIVFNDMNKIIDYSYSVDKVPYIKAGTTIDTIPVEYKLFRWFGPTKKIDVPFVA
TENIDYYKNYVNEKETSKSINLNDMNAWQLASNPESAAVTVTQRAYVKDYPVKADIGTFTLIKANFLSYLGIIVLVVVAI
VLILLIIRAINLRKRRRRRRNIF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=326, Percent_Identity=32.2085889570552, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786851, Length=321, Percent_Identity=29.9065420560748, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082022, Length=294, Percent_Identity=28.9115646258503, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082054, Length=254, Percent_Identity=31.1023622047244, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46896; Mature: 46896

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFKKKLLSSILIALSVSLFTPTKATVQAAEISQPNIVGKYAVTLDYDTGEIIYAKGID
CCCHHHHHHHHHHHHHHHHHCCCCHHHEEEHHCCCCCEEEEEEEEEECCCCCEEEEECCC
EKAYPASTTKVMTSLLFAEHASKNDSFPYTADAKAQQPYTLNNSFGPIPVGEGMNANDLM
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHH
KALLMFSANDAAAVIADGVAGSAEKFSVMMNDEVKKLGLKNTHFVTPNGLHNDDHYSTAY
HHHHHHCCCCCEEEEECCCCCCHHHEEEEECCHHHHCCCCCCEEECCCCCCCCCCCHHHH
DLAVILQNAYKNPWVSETMALKDSDITVNGKKVLLENRNKELGIDGNIGGKTGFTTPAGR
HHHHHHHHHCCCCCCCHHEEECCCCEEECCCEEEEECCCCEECCCCCCCCCCCCCCCHHH
CLVSVYERNGRKIIGAVLNSQYDAKDEIVFNDMNKIIDYSYSVDKVPYIKAGTTIDTIPV
HHHHHHHCCCCEEEEHHHCCCCCCCCCEEHHHHHHHHCCCCCCCCCCEEECCCEEEECCC
EYKLFRWFGPTKKIDVPFVATENIDYYKNYVNEKETSKSINLNDMNAWQLASNPESAAVT
CEEEEEEECCCCCCCCCEEEECCCHHHHHHCCHHHCCCCCCCCCCCCEEECCCCCCCEEE
VTQRAYVKDYPVKADIGTFTLIKANFLSYLGIIVLVVVAIVLILLIIRAINLRKRRRRRR
EEEEHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIF
CCC
>Mature Secondary Structure
MIKFKKKLLSSILIALSVSLFTPTKATVQAAEISQPNIVGKYAVTLDYDTGEIIYAKGID
CCCHHHHHHHHHHHHHHHHHCCCCHHHEEEHHCCCCCEEEEEEEEEECCCCCEEEEECCC
EKAYPASTTKVMTSLLFAEHASKNDSFPYTADAKAQQPYTLNNSFGPIPVGEGMNANDLM
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHH
KALLMFSANDAAAVIADGVAGSAEKFSVMMNDEVKKLGLKNTHFVTPNGLHNDDHYSTAY
HHHHHHCCCCCEEEEECCCCCCHHHEEEEECCHHHHCCCCCCEEECCCCCCCCCCCHHHH
DLAVILQNAYKNPWVSETMALKDSDITVNGKKVLLENRNKELGIDGNIGGKTGFTTPAGR
HHHHHHHHHCCCCCCCHHEEECCCCEEECCCEEEEECCCCEECCCCCCCCCCCCCCCHHH
CLVSVYERNGRKIIGAVLNSQYDAKDEIVFNDMNKIIDYSYSVDKVPYIKAGTTIDTIPV
HHHHHHHCCCCEEEEHHHCCCCCCCCCEEHHHHHHHHCCCCCCCCCCEEECCCEEEECCC
EYKLFRWFGPTKKIDVPFVATENIDYYKNYVNEKETSKSINLNDMNAWQLASNPESAAVT
CEEEEEEECCCCCCCCCEEEECCCHHHHHHCCHHHCCCCCCCCCCCCEEECCCCCCCEEE
VTQRAYVKDYPVKADIGTFTLIKANFLSYLGIIVLVVVAIVLILLIIRAINLRKRRRRRR
EEEEHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NIF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]