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The map label for this gene is murE

Identifier: 18310844

GI number: 18310844

Start: 2130930

End: 2132384

Strand: Reverse

Name: murE

Synonym: CPE1862

Alternate gene names: 18310844

Gene position: 2132384-2130930 (Counterclockwise)

Preceding gene: 18310845

Following gene: 18310843

Centisome position: 70.34

GC content: 29.48

Gene sequence:

>1455_bases
ATGATTCTTAAAAGTTTATTAAAGGGCTTAGATTATGAAGTTATAAAAGGTAATGAAGAGTCAAAAGTACAAAATATAAG
ATATGATAACAGAAAAATAGAACAAGGGGATGCCTTTGTTTGTGTAAAAGGCTTTAAAGTAGATGGACATTCCTTTATTG
GTGATGCTATTAAAAAAGGAGCTAAGACATTAATAGTTCAAGAAGATGTAAGTGTTCAAGAAGACATAACAATAATAAAG
GTTAGAGACACTAGAAAAGCCTTGGCAATAATGTCTTCAAATTATTTTGGAAATCCAAAGGATAAACTTAAAATAATAGG
AATAACAGGAACTAATGGTAAAACCACTTCTGCTTTTATTATTAAATCTATCTTAGAAAAGGCAGGATTTATGACTGGTT
TAATAGGAACCATAGCAAACTATATAGGAAATAAAAAAGTAGACGCGGTAAGAACTACACCAGAATCTTACGAGCTTCAT
GAGCTATTTAAAAATATGGTTGATGCTGGAGTAGAATATTGCGTTATGGAAGTTTCATCCCACTCTCTAGAGTTAGATAG
AGTATATGGAATTCAATTTGAAGAAGGAATTTTCACTAATTTAACTAGAGATCATTTAGATTTTCATAAAACCTTTGAAA
ACTATTATAATGCTAAATTTAAGCTTTTTGAAAGAAGTAATCATAGTATCATAAACTTAGATGATCCATATGGAGCTAAT
ATAGTTAAGGATATAGAAGAAAGAGGAGTAAAGACTAAGGTTTCTACTTTTTCAATTGAAAAAGAGTCAGACTTTAAGGC
TTTTGAGATTAAAAGTCACTCAAATGGTTCAGAATTTAAGGTGAATTTAGAAGGTATAGAAGAATTTTCTATAAACATTC
CTGGAGAATACAATATATATAACTCATTAGGTTGTATAATATGTGCATATAACTTAAATATTCCAATGGATAAAATTAAA
GAAGGATTAAGTGATGTGGTTATACCAGGAAGATGTGAGTTAGTAGCAAAGGAGAAAAATTTACCTTATAGCATAATAAT
AGATTATGCACACACACCAGATGGATTAGAAAATATTTTAAGTACAGTAAAGGCCTTTACTAAAAATAGAATGATTTCTG
TATTTGGTTGTGGAGGAGATAGAGATAAGGTTAAGCGTCCTCAAATGGGTAAAATAGGATGTGAATTATCAGATATTGCA
ATAATTACTTCAGATAATCCAAGAAGTGAGGAGCCTATGGATATAATTAATGACATAGTAAAACCACTTAATTATGATAA
CTTTGTTATTGAAGTTAATAGAAAAGAAGCTATAAGAAAAGCAATGAATATGGCTTTAGAGGGAGATGTTATTGTTATAG
CTGGTAAAGGACATGAAACATATCAAATACTAAAGGATGAAACAATACACTTTGATGAAAGAGAAGTTGTATATGATATT
TTAGAAGGCAAGTAG

Upstream 100 bases:

>100_bases
AAATAATGATAGTTAAAAACTAGCATGTGAGTAATTTCACATGCTAGTATTTTGTGAATTTAACGCTTGTTTATTGCATA
GTATTTAAGGAGTGAGAAAA

Downstream 100 bases:

>100_bases
AGGTGTTAAAAAATGCTAGAGTTAAATTTACAAGAGATTGTAAAAGCTACTAAGGGAGCTTTATTAAAAGAAGCTGATGT
AAAAGAAATAAAGGCTGTAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MILKSLLKGLDYEVIKGNEESKVQNIRYDNRKIEQGDAFVCVKGFKVDGHSFIGDAIKKGAKTLIVQEDVSVQEDITIIK
VRDTRKALAIMSSNYFGNPKDKLKIIGITGTNGKTTSAFIIKSILEKAGFMTGLIGTIANYIGNKKVDAVRTTPESYELH
ELFKNMVDAGVEYCVMEVSSHSLELDRVYGIQFEEGIFTNLTRDHLDFHKTFENYYNAKFKLFERSNHSIINLDDPYGAN
IVKDIEERGVKTKVSTFSIEKESDFKAFEIKSHSNGSEFKVNLEGIEEFSINIPGEYNIYNSLGCIICAYNLNIPMDKIK
EGLSDVVIPGRCELVAKEKNLPYSIIIDYAHTPDGLENILSTVKAFTKNRMISVFGCGGDRDKVKRPQMGKIGCELSDIA
IITSDNPRSEEPMDIINDIVKPLNYDNFVIEVNRKEAIRKAMNMALEGDVIVIAGKGHETYQILKDETIHFDEREVVYDI
LEGK

Sequences:

>Translated_484_residues
MILKSLLKGLDYEVIKGNEESKVQNIRYDNRKIEQGDAFVCVKGFKVDGHSFIGDAIKKGAKTLIVQEDVSVQEDITIIK
VRDTRKALAIMSSNYFGNPKDKLKIIGITGTNGKTTSAFIIKSILEKAGFMTGLIGTIANYIGNKKVDAVRTTPESYELH
ELFKNMVDAGVEYCVMEVSSHSLELDRVYGIQFEEGIFTNLTRDHLDFHKTFENYYNAKFKLFERSNHSIINLDDPYGAN
IVKDIEERGVKTKVSTFSIEKESDFKAFEIKSHSNGSEFKVNLEGIEEFSINIPGEYNIYNSLGCIICAYNLNIPMDKIK
EGLSDVVIPGRCELVAKEKNLPYSIIIDYAHTPDGLENILSTVKAFTKNRMISVFGCGGDRDKVKRPQMGKIGCELSDIA
IITSDNPRSEEPMDIINDIVKPLNYDNFVIEVNRKEAIRKAMNMALEGDVIVIAGKGHETYQILKDETIHFDEREVVYDI
LEGK
>Mature_484_residues
MILKSLLKGLDYEVIKGNEESKVQNIRYDNRKIEQGDAFVCVKGFKVDGHSFIGDAIKKGAKTLIVQEDVSVQEDITIIK
VRDTRKALAIMSSNYFGNPKDKLKIIGITGTNGKTTSAFIIKSILEKAGFMTGLIGTIANYIGNKKVDAVRTTPESYELH
ELFKNMVDAGVEYCVMEVSSHSLELDRVYGIQFEEGIFTNLTRDHLDFHKTFENYYNAKFKLFERSNHSIINLDDPYGAN
IVKDIEERGVKTKVSTFSIEKESDFKAFEIKSHSNGSEFKVNLEGIEEFSINIPGEYNIYNSLGCIICAYNLNIPMDKIK
EGLSDVVIPGRCELVAKEKNLPYSIIIDYAHTPDGLENILSTVKAFTKNRMISVFGCGGDRDKVKRPQMGKIGCELSDIA
IITSDNPRSEEPMDIINDIVKPLNYDNFVIEVNRKEAIRKAMNMALEGDVIVIAGKGHETYQILKDETIHFDEREVVYDI
LEGK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=478, Percent_Identity=32.6359832635983, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1786274, Length=314, Percent_Identity=27.0700636942675, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CLOPE (Q8XJ99)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562778.1
- ProteinModelPortal:   Q8XJ99
- SMR:   Q8XJ99
- GeneID:   990171
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1862
- NMPDR:   fig|195102.1.peg.1925
- HOGENOM:   HBG602753
- OMA:   IGTIANY
- PhylomeDB:   Q8XJ99
- ProtClustDB:   PRK00139
- BioCyc:   CPER195102:CPE1862-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54472; Mature: 54472

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: BINDING 179-179 BINDING 187-187 BINDING 381-381 BINDING 455-455 BINDING 459-459

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKSLLKGLDYEVIKGNEESKVQNIRYDNRKIEQGDAFVCVKGFKVDGHSFIGDAIKKG
CCHHHHHCCCCEEEECCCCHHHHHHCCCCCCEECCCCEEEEEEEEEECCHHHHHHHHHCC
AKTLIVQEDVSVQEDITIIKVRDTRKALAIMSSNYFGNPKDKLKIIGITGTNGKTTSAFI
CEEEEEECCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHH
IKSILEKAGFMTGLIGTIANYIGNKKVDAVRTTPESYELHELFKNMVDAGVEYCVMEVSS
HHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCHHEEEEECCC
HSLELDRVYGIQFEEGIFTNLTRDHLDFHKTFENYYNAKFKLFERSNHSIINLDDPYGAN
CCEEEEEEECEEECCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCH
IVKDIEERGVKTKVSTFSIEKESDFKAFEIKSHSNGSEFKVNLEGIEEFSINIPGEYNIY
HHHHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCC
NSLGCIICAYNLNIPMDKIKEGLSDVVIPGRCELVAKEKNLPYSIIIDYAHTPDGLENIL
CCCCEEEEEEECCCCHHHHHCCHHHEECCCCEEEEEECCCCCEEEEEEECCCCHHHHHHH
STVKAFTKNRMISVFGCGGDRDKVKRPQMGKIGCELSDIAIITSDNPRSEEPMDIINDIV
HHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
KPLNYDNFVIEVNRKEAIRKAMNMALEGDVIVIAGKGHETYQILKDETIHFDEREVVYDI
HHCCCCCEEEEECHHHHHHHHHHHEECCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
LEGK
HCCC
>Mature Secondary Structure
MILKSLLKGLDYEVIKGNEESKVQNIRYDNRKIEQGDAFVCVKGFKVDGHSFIGDAIKKG
CCHHHHHCCCCEEEECCCCHHHHHHCCCCCCEECCCCEEEEEEEEEECCHHHHHHHHHCC
AKTLIVQEDVSVQEDITIIKVRDTRKALAIMSSNYFGNPKDKLKIIGITGTNGKTTSAFI
CEEEEEECCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHH
IKSILEKAGFMTGLIGTIANYIGNKKVDAVRTTPESYELHELFKNMVDAGVEYCVMEVSS
HHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCHHEEEEECCC
HSLELDRVYGIQFEEGIFTNLTRDHLDFHKTFENYYNAKFKLFERSNHSIINLDDPYGAN
CCEEEEEEECEEECCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCH
IVKDIEERGVKTKVSTFSIEKESDFKAFEIKSHSNGSEFKVNLEGIEEFSINIPGEYNIY
HHHHHHHCCCEEEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCEEEEEECCCCCCCC
NSLGCIICAYNLNIPMDKIKEGLSDVVIPGRCELVAKEKNLPYSIIIDYAHTPDGLENIL
CCCCEEEEEEECCCCHHHHHCCHHHEECCCCEEEEEECCCCCEEEEEEECCCCHHHHHHH
STVKAFTKNRMISVFGCGGDRDKVKRPQMGKIGCELSDIAIITSDNPRSEEPMDIINDIV
HHHHHHHCCCEEEEEECCCCHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHH
KPLNYDNFVIEVNRKEAIRKAMNMALEGDVIVIAGKGHETYQILKDETIHFDEREVVYDI
HHCCCCCEEEEECHHHHHHHHHHHEECCCEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
LEGK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842