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The map label for this gene is dacB [H]

Identifier: 18310783

GI number: 18310783

Start: 2081174

End: 2082049

Strand: Reverse

Name: dacB [H]

Synonym: CPE1801

Alternate gene names: 18310783

Gene position: 2082049-2081174 (Counterclockwise)

Preceding gene: 18310786

Following gene: 18310782

Centisome position: 68.68

GC content: 32.31

Gene sequence:

>876_bases
ATGAAAAAGAAGATTTCAATTCTTATGTTTTTTTTAATAACAATATCTATATGTTCTAATGTTATTGTTCTTGGAAAGGA
AAGTTGTATTCTAGAGGAAGTTTCAGTAGAGACTATGGATGTAAAAGATGGATATGGAAAGCAGTGCAAGGATATTACTC
CTGATGCACATTTTGCTATTGCCTATGATGCTAAAAATAAAAAGATTTTATATGAAAAGAATGCTTTTAGAAATGTACCT
ATGGCAAGTACCACTAAAATATTAACTGCTTTAGTTGCCATAAATTATTCTGACTTAGATGAAGAGGTTACAATATCTAA
AACTGCAGCAAGTATTAGAGGATCAAAAGTTGGATTTAGGGCTGGAGAAAAGGTTACAATGAGAGAACTTCTTTTTGGGC
TTATGTACAAGTCAGGAAATGATGCTGCCATAGCTATTGCAGAGCATATTGGTGGTAGTATAGAGAACTTTTCTATACTT
ATGAATGATTTTGCTAGAATGTTAGGTCTTAAGGATTCAAACTTTCAATCTCCTCATGGATTAGATAGCCAAATGCATTT
TTCCTCTGCTTATGATTTAGCGGTTTTAATGTCAAAAGCTATGGAAGAGCCTTTCTTTAGAGAGATATCAGGAACTAAGG
AAGTAGGAGCTGAAAAATATAATTTTACTAGAAGTTATAGCAATATAAATAAAATGCTTTGGAGATTACCTAATGCCAAT
GGAGGAAAAACAGGCTATACTGGTCAGGCAGGGAAATGTTTAGTAAGTTCTGTAGATCATAATGGAAGAAATATAATCAT
AGTAGTTTTAAATTGTCCAACTAGGTGGGAAGCCACTGAAAGAGTTTATGAATATGTTAAAGCCAATTATTCTTAA

Upstream 100 bases:

>100_bases
AATTATCCAAAAATATACTAGGTTATTAAATTTATAAAGTGGAAAGCTAATAAAAGAAGGTTTCATAGATAAAAAAGCTT
AGTTTAAGGAGAGATATTTT

Downstream 100 bases:

>100_bases
AATCAAAGGTGTTATATTAATATTTTAATATAACACCTTTGATTTTTTTGTTTTAAAGTTTAAAAAATATATTATTTTGT
TATATATAAATGCTTAATAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKKKISILMFFLITISICSNVIVLGKESCILEEVSVETMDVKDGYGKQCKDITPDAHFAIAYDAKNKKILYEKNAFRNVP
MASTTKILTALVAINYSDLDEEVTISKTAASIRGSKVGFRAGEKVTMRELLFGLMYKSGNDAAIAIAEHIGGSIENFSIL
MNDFARMLGLKDSNFQSPHGLDSQMHFSSAYDLAVLMSKAMEEPFFREISGTKEVGAEKYNFTRSYSNINKMLWRLPNAN
GGKTGYTGQAGKCLVSSVDHNGRNIIIVVLNCPTRWEATERVYEYVKANYS

Sequences:

>Translated_291_residues
MKKKISILMFFLITISICSNVIVLGKESCILEEVSVETMDVKDGYGKQCKDITPDAHFAIAYDAKNKKILYEKNAFRNVP
MASTTKILTALVAINYSDLDEEVTISKTAASIRGSKVGFRAGEKVTMRELLFGLMYKSGNDAAIAIAEHIGGSIENFSIL
MNDFARMLGLKDSNFQSPHGLDSQMHFSSAYDLAVLMSKAMEEPFFREISGTKEVGAEKYNFTRSYSNINKMLWRLPNAN
GGKTGYTGQAGKCLVSSVDHNGRNIIIVVLNCPTRWEATERVYEYVKANYS
>Mature_291_residues
MKKKISILMFFLITISICSNVIVLGKESCILEEVSVETMDVKDGYGKQCKDITPDAHFAIAYDAKNKKILYEKNAFRNVP
MASTTKILTALVAINYSDLDEEVTISKTAASIRGSKVGFRAGEKVTMRELLFGLMYKSGNDAAIAIAEHIGGSIENFSIL
MNDFARMLGLKDSNFQSPHGLDSQMHFSSAYDLAVLMSKAMEEPFFREISGTKEVGAEKYNFTRSYSNINKMLWRLPNAN
GGKTGYTGQAGKCLVSSVDHNGRNIIIVVLNCPTRWEATERVYEYVKANYS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=247, Percent_Identity=36.0323886639676, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87082022, Length=257, Percent_Identity=33.4630350194552, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786851, Length=235, Percent_Identity=33.6170212765957, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082054, Length=220, Percent_Identity=30.4545454545455, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 32348; Mature: 32348

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKISILMFFLITISICSNVIVLGKESCILEEVSVETMDVKDGYGKQCKDITPDAHFAI
CCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEE
AYDAKNKKILYEKNAFRNVPMASTTKILTALVAINYSDLDEEVTISKTAASIRGSKVGFR
EEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCCCC
AGEKVTMRELLFGLMYKSGNDAAIAIAEHIGGSIENFSILMNDFARMLGLKDSNFQSPHG
CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LDSQMHFSSAYDLAVLMSKAMEEPFFREISGTKEVGAEKYNFTRSYSNINKMLWRLPNAN
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCC
GGKTGYTGQAGKCLVSSVDHNGRNIIIVVLNCPTRWEATERVYEYVKANYS
CCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKKISILMFFLITISICSNVIVLGKESCILEEVSVETMDVKDGYGKQCKDITPDAHFAI
CCCHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEE
AYDAKNKKILYEKNAFRNVPMASTTKILTALVAINYSDLDEEVTISKTAASIRGSKVGFR
EEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCCCCCCC
AGEKVTMRELLFGLMYKSGNDAAIAIAEHIGGSIENFSILMNDFARMLGLKDSNFQSPHG
CCCCHHHHHHHHHHHHCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LDSQMHFSSAYDLAVLMSKAMEEPFFREISGTKEVGAEKYNFTRSYSNINKMLWRLPNAN
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCC
GGKTGYTGQAGKCLVSSVDHNGRNIIIVVLNCPTRWEATERVYEYVKANYS
CCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]