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The map label for this gene is alaS [H]

Identifier: 18310762

GI number: 18310762

Start: 2057656

End: 2060295

Strand: Reverse

Name: alaS [H]

Synonym: CPE1780

Alternate gene names: 18310762

Gene position: 2060295-2057656 (Counterclockwise)

Preceding gene: 18310763

Following gene: 18310761

Centisome position: 67.96

GC content: 31.59

Gene sequence:

>2640_bases
ATGAAATTCATGGGAGCAAATGAATTAAGAGAAAAATATTTAAGCTTTTTTGAAAGCAAAGATCATTTAAGATTACAGTC
ATTTCCGTTAGTACCTAAAAATGATAAGAGTTTATTATTAATAAATGCAGGTATGGCACCACTTAAACCTTATTTCACAG
GTTTAGAAGAACCACCAAAAAGAAGAATAACAACTTGTCAAAAGTGTATAAGAACTGGTGATATAGAGAATGTTGGTAAA
ACATCAAGACATGGTACATTCTTCGAAATGTTAGGAAATTTCTCATTTGGAGATTACTTCAAATCAGAAATAATTCCATG
GGCTTGGGAGTTTATAACAGAAACTTTAGGAATTCCAAAGGATAAATTATATGTAACTATATATTTAAATGATGATGAAG
CTTATGACATTTGGACTAGTAAAACTGATGTGGATCCAAGCAGAATATTTAGATTAGGGAAAGATGATAACTTCTGGGAA
ATAGGGGTAGGTCCTTGTGGTCCTTGTACAGAGATTCACTTTGATAGAGGAGAAGGAAAGGTTGAAACTGTAGAAGAATT
CTTAGAAGCTTCAGATGCAGATAGAATAGTTGAATTCTGGAACTTAGTTTTCACTCAATTTGATAAAGATGAAGAAGGAA
ACTACAATGAGTTAGCTCAAAAGAATATAGATACAGGTATGGGATTAGAAAGAATAGCTACAATAATGCAAGGTGTAGAT
AATATCTTCGAAATAGATACAGTTAAAAACATATTAAATAAAGCATGTGAATTAACAAATGCTAAATATGGAGAAGATAA
AGACAAAGATGTATCATTAAGAATAATAACTGACCATGGAAAAAGTGTTACTTTCTTAATATGTGACGGTGTTCAACCAT
CAAATGAAGGTAGAGGATATGTTTTAAGAAGACTTCTTAGAAGAGCTGCTAGACATGGAAGACTTTTAGGAGTTAAAGGT
ATATTCTTAAATGAAATGGTTGATGCTGTAGTTGAAAATTACGGTGAAGCTTATCCAGAATTAAGAGAAAAAGCTGATTA
CATTAAGAAGATAATCAAGTTAGAAGAAGAAAGATTTAATGAAACAATAGACTCAGGTATGGACATACTTATGAGCTATA
TTTCAGAAATGGAAGAAAAAAATGAGAAAGTTTTATCAGGAGCTAAAGCTTTCAAATTATATGATACTTATGGATTCCCT
CTAGAGCTTACTCAAGAGATATTAGAAGAAAAAGGCTTAGAGTTAGATATAGAAAACTTTAATAAGGAAATGAAAGAGCA
AAGAGAAAGAGCTAGAAATGCTAGAGGAGAAAGTAGCTACATGGGAAGCGAAGAAAGTCCAGTAAACAAAGTGGATGCTT
CAATAGTTACTGAATTTGATGGATATGTTAATTTAGAACTTAACTCAAAAGTTATAGTACTAGGAAATAATGAAGAATTT
AAAGCTGAACTTAAAGAAGGTGAAGAAGGATTCTTATTAACTGATAAAACTCCTTTCTATGCTGAAATGGGAGGTCAAGT
TGGAGATAGAGGAAATATAACTTCAGAAACTGGAATGGCAATAGTTACAGATTGTAAGAAAAATGTTGGTGGAAAATTTG
TTCACTACATTAAGGTTATAGAAGGTAGTTTAAAAGAAGGTCAAGAAGTTAAATTATCAGTTGATGCTTCAAGAAGATCT
AACATATGTAAAAACCACACAGCTACACACATGTTACATGAAGCTTTAAAAGAAGTTTTAGGAGATCATGTAAATCAATC
AGGTTCATATGTTGATGAAGAAAGATTAAGATTTGACTTTACTCATTTTGCAGCTTTAACAGAAGAAGAATTAGAAAAAG
TTGAATTATTAGTAAATGAAAAAATAATGACTGTATCTGTAGTTGATACAAAGGAAATGTCATTAGATGAAGCTAGAAAT
AGTGGAGCAACTTGTCTTTTCGATGAAAAGTATGCTGAAAAAGTAAGAGTTGTTTCAGTTGGAGATTTCTCAAAAGAATT
ATGTGGAGGAACTCACGTAGCTAACTCAGGAGAAATTGGATTATTTAAGATAGTTTCAGAATCAGGAGTTGCTGCTGGAA
TAAGAAGAATTGAGGCTGTTACTGGAATAAGTGCATTAAAATTCATGGAACTTAAAAATAATATGCTTAAGGAAGCTGCT
TCAATGCTTAAGTGTAATGAAAAAGATATTGCTAAGAGAATAGCAGCTCAAGCTCATGAATTAAAAGAAAAAGATAAAGA
AATAGCAGAACTTAAAGCTAAATTAGTTCAAGGTGCTGAGGATGATATCTTAAAAGACAAAGTTGAAATAAACGGAGTTG
AATTAGTTACAGCAGAATTAAAAGATGTTGATGGAAATTCATTAAGAGATTTAGCTGATAAAGTTAGAAATAAATTAAAT
AATGGAATAGTTGTTTTAGCAAGTGACAATGGTGGAAAAGTAAACTTAGTAGCTATGGCAACTAAAAATTCATTAGCTAA
TGGAGTTCATTGTGGAAAGGTAATAAAAGAAGTTGCAGCAGTTGTAGGCGGCGGCGGAGGTGGAAGACCTGACATGGCTC
AAGCTGGTGGAAAAAATCCAGAAAATATAGCTAAAGCATTAGAAAAAGCAAAAGAAGTTGTAGAATTACTTGTAAAATAG

Upstream 100 bases:

>100_bases
AGAATATCTAGGCTAAGTAGAGGGTTTAATCTTATTTTATAAATAGAGGATTAAACTTAAAGGCTAAAGGATAAATACAT
AAAATTTGGAGGAATAAATT

Downstream 100 bases:

>100_bases
TTGTACTTTTATGTAAATATAGTTTATAATGAGTATAAAGTAAAGCGTGAATAAAAACTAATTTAAAAGATAATAATATA
TGTAAATATATAAATTAAAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 879; Mature: 879

Protein sequence:

>879_residues
MKFMGANELREKYLSFFESKDHLRLQSFPLVPKNDKSLLLINAGMAPLKPYFTGLEEPPKRRITTCQKCIRTGDIENVGK
TSRHGTFFEMLGNFSFGDYFKSEIIPWAWEFITETLGIPKDKLYVTIYLNDDEAYDIWTSKTDVDPSRIFRLGKDDNFWE
IGVGPCGPCTEIHFDRGEGKVETVEEFLEASDADRIVEFWNLVFTQFDKDEEGNYNELAQKNIDTGMGLERIATIMQGVD
NIFEIDTVKNILNKACELTNAKYGEDKDKDVSLRIITDHGKSVTFLICDGVQPSNEGRGYVLRRLLRRAARHGRLLGVKG
IFLNEMVDAVVENYGEAYPELREKADYIKKIIKLEEERFNETIDSGMDILMSYISEMEEKNEKVLSGAKAFKLYDTYGFP
LELTQEILEEKGLELDIENFNKEMKEQRERARNARGESSYMGSEESPVNKVDASIVTEFDGYVNLELNSKVIVLGNNEEF
KAELKEGEEGFLLTDKTPFYAEMGGQVGDRGNITSETGMAIVTDCKKNVGGKFVHYIKVIEGSLKEGQEVKLSVDASRRS
NICKNHTATHMLHEALKEVLGDHVNQSGSYVDEERLRFDFTHFAALTEEELEKVELLVNEKIMTVSVVDTKEMSLDEARN
SGATCLFDEKYAEKVRVVSVGDFSKELCGGTHVANSGEIGLFKIVSESGVAAGIRRIEAVTGISALKFMELKNNMLKEAA
SMLKCNEKDIAKRIAAQAHELKEKDKEIAELKAKLVQGAEDDILKDKVEINGVELVTAELKDVDGNSLRDLADKVRNKLN
NGIVVLASDNGGKVNLVAMATKNSLANGVHCGKVIKEVAAVVGGGGGGRPDMAQAGGKNPENIAKALEKAKEVVELLVK

Sequences:

>Translated_879_residues
MKFMGANELREKYLSFFESKDHLRLQSFPLVPKNDKSLLLINAGMAPLKPYFTGLEEPPKRRITTCQKCIRTGDIENVGK
TSRHGTFFEMLGNFSFGDYFKSEIIPWAWEFITETLGIPKDKLYVTIYLNDDEAYDIWTSKTDVDPSRIFRLGKDDNFWE
IGVGPCGPCTEIHFDRGEGKVETVEEFLEASDADRIVEFWNLVFTQFDKDEEGNYNELAQKNIDTGMGLERIATIMQGVD
NIFEIDTVKNILNKACELTNAKYGEDKDKDVSLRIITDHGKSVTFLICDGVQPSNEGRGYVLRRLLRRAARHGRLLGVKG
IFLNEMVDAVVENYGEAYPELREKADYIKKIIKLEEERFNETIDSGMDILMSYISEMEEKNEKVLSGAKAFKLYDTYGFP
LELTQEILEEKGLELDIENFNKEMKEQRERARNARGESSYMGSEESPVNKVDASIVTEFDGYVNLELNSKVIVLGNNEEF
KAELKEGEEGFLLTDKTPFYAEMGGQVGDRGNITSETGMAIVTDCKKNVGGKFVHYIKVIEGSLKEGQEVKLSVDASRRS
NICKNHTATHMLHEALKEVLGDHVNQSGSYVDEERLRFDFTHFAALTEEELEKVELLVNEKIMTVSVVDTKEMSLDEARN
SGATCLFDEKYAEKVRVVSVGDFSKELCGGTHVANSGEIGLFKIVSESGVAAGIRRIEAVTGISALKFMELKNNMLKEAA
SMLKCNEKDIAKRIAAQAHELKEKDKEIAELKAKLVQGAEDDILKDKVEINGVELVTAELKDVDGNSLRDLADKVRNKLN
NGIVVLASDNGGKVNLVAMATKNSLANGVHCGKVIKEVAAVVGGGGGGRPDMAQAGGKNPENIAKALEKAKEVVELLVK
>Mature_879_residues
MKFMGANELREKYLSFFESKDHLRLQSFPLVPKNDKSLLLINAGMAPLKPYFTGLEEPPKRRITTCQKCIRTGDIENVGK
TSRHGTFFEMLGNFSFGDYFKSEIIPWAWEFITETLGIPKDKLYVTIYLNDDEAYDIWTSKTDVDPSRIFRLGKDDNFWE
IGVGPCGPCTEIHFDRGEGKVETVEEFLEASDADRIVEFWNLVFTQFDKDEEGNYNELAQKNIDTGMGLERIATIMQGVD
NIFEIDTVKNILNKACELTNAKYGEDKDKDVSLRIITDHGKSVTFLICDGVQPSNEGRGYVLRRLLRRAARHGRLLGVKG
IFLNEMVDAVVENYGEAYPELREKADYIKKIIKLEEERFNETIDSGMDILMSYISEMEEKNEKVLSGAKAFKLYDTYGFP
LELTQEILEEKGLELDIENFNKEMKEQRERARNARGESSYMGSEESPVNKVDASIVTEFDGYVNLELNSKVIVLGNNEEF
KAELKEGEEGFLLTDKTPFYAEMGGQVGDRGNITSETGMAIVTDCKKNVGGKFVHYIKVIEGSLKEGQEVKLSVDASRRS
NICKNHTATHMLHEALKEVLGDHVNQSGSYVDEERLRFDFTHFAALTEEELEKVELLVNEKIMTVSVVDTKEMSLDEARN
SGATCLFDEKYAEKVRVVSVGDFSKELCGGTHVANSGEIGLFKIVSESGVAAGIRRIEAVTGISALKFMELKNNMLKEAA
SMLKCNEKDIAKRIAAQAHELKEKDKEIAELKAKLVQGAEDDILKDKVEINGVELVTAELKDVDGNSLRDLADKVRNKLN
NGIVVLASDNGGKVNLVAMATKNSLANGVHCGKVIKEVAAVVGGGGGGRPDMAQAGGKNPENIAKALEKAKEVVELLVK

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=977, Percent_Identity=34.6980552712385, Blast_Score=495, Evalue=1e-140,
Organism=Homo sapiens, GI38569417, Length=788, Percent_Identity=32.8680203045685, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI217416402, Length=250, Percent_Identity=26.8, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI13376886, Length=250, Percent_Identity=26.8, Blast_Score=74, Evalue=9e-13,
Organism=Escherichia coli, GI1789048, Length=870, Percent_Identity=43.448275862069, Blast_Score=720, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=969, Percent_Identity=34.2621259029928, Blast_Score=490, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17536681, Length=735, Percent_Identity=33.1972789115646, Blast_Score=367, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324911, Length=756, Percent_Identity=39.021164021164, Blast_Score=496, Evalue=1e-141,
Organism=Drosophila melanogaster, GI24582809, Length=974, Percent_Identity=34.8049281314168, Blast_Score=510, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45552267, Length=974, Percent_Identity=34.8049281314168, Blast_Score=510, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24658214, Length=822, Percent_Identity=29.5620437956204, Blast_Score=296, Evalue=6e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 98085; Mature: 98085

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFMGANELREKYLSFFESKDHLRLQSFPLVPKNDKSLLLINAGMAPLKPYFTGLEEPPK
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCHH
RRITTCQKCIRTGDIENVGKTSRHGTFFEMLGNFSFGDYFKSEIIPWAWEFITETLGIPK
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCCC
DKLYVTIYLNDDEAYDIWTSKTDVDPSRIFRLGKDDNFWEIGVGPCGPCTEIHFDRGEGK
CCEEEEEEECCCCCEEEECCCCCCCHHHHEECCCCCCEEEECCCCCCCCCCEEEECCCCH
VETVEEFLEASDADRIVEFWNLVFTQFDKDEEGNYNELAQKNIDTGMGLERIATIMQGVD
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
NIFEIDTVKNILNKACELTNAKYGEDKDKDVSLRIITDHGKSVTFLICDGVQPSNEGRGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCHH
VLRRLLRRAARHGRLLGVKGIFLNEMVDAVVENYGEAYPELREKADYIKKIIKLEEERFN
HHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIDSGMDILMSYISEMEEKNEKVLSGAKAFKLYDTYGFPLELTQEILEEKGLELDIENF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHCCCEEEHHHH
NKEMKEQRERARNARGESSYMGSEESPVNKVDASIVTEFDGYVNLELNSKVIVLGNNEEF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHEECCCEEEEEECCEEEEECCCHHH
KAELKEGEEGFLLTDKTPFYAEMGGQVGDRGNITSETGMAIVTDCKKNVGGKFVHYIKVI
HHHHCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHH
EGSLKEGQEVKLSVDASRRSNICKNHTATHMLHEALKEVLGDHVNQSGSYVDEERLRFDF
HHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
THFAALTEEELEKVELLVNEKIMTVSVVDTKEMSLDEARNSGATCLFDEKYAEKVRVVSV
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEEECHHHHCCEEEEEE
GDFSKELCGGTHVANSGEIGLFKIVSESGVAAGIRRIEAVTGISALKFMELKNNMLKEAA
CCHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMLKCNEKDIAKRIAAQAHELKEKDKEIAELKAKLVQGAEDDILKDKVEINGVELVTAEL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCEEEEEEEH
KDVDGNSLRDLADKVRNKLNNGIVVLASDNGGKVNLVAMATKNSLANGVHCGKVIKEVAA
HCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCHHHCCHHHHHHHHHHHH
VVGGGGGGRPDMAQAGGKNPENIAKALEKAKEVVELLVK
HHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFMGANELREKYLSFFESKDHLRLQSFPLVPKNDKSLLLINAGMAPLKPYFTGLEEPPK
CCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCHH
RRITTCQKCIRTGDIENVGKTSRHGTFFEMLGNFSFGDYFKSEIIPWAWEFITETLGIPK
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCCC
DKLYVTIYLNDDEAYDIWTSKTDVDPSRIFRLGKDDNFWEIGVGPCGPCTEIHFDRGEGK
CCEEEEEEECCCCCEEEECCCCCCCHHHHEECCCCCCEEEECCCCCCCCCCEEEECCCCH
VETVEEFLEASDADRIVEFWNLVFTQFDKDEEGNYNELAQKNIDTGMGLERIATIMQGVD
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHH
NIFEIDTVKNILNKACELTNAKYGEDKDKDVSLRIITDHGKSVTFLICDGVQPSNEGRGY
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCHH
VLRRLLRRAARHGRLLGVKGIFLNEMVDAVVENYGEAYPELREKADYIKKIIKLEEERFN
HHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ETIDSGMDILMSYISEMEEKNEKVLSGAKAFKLYDTYGFPLELTQEILEEKGLELDIENF
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHCCCEEEHHHH
NKEMKEQRERARNARGESSYMGSEESPVNKVDASIVTEFDGYVNLELNSKVIVLGNNEEF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHEECCCEEEEEECCEEEEECCCHHH
KAELKEGEEGFLLTDKTPFYAEMGGQVGDRGNITSETGMAIVTDCKKNVGGKFVHYIKVI
HHHHCCCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCCHHHHHHHHHH
EGSLKEGQEVKLSVDASRRSNICKNHTATHMLHEALKEVLGDHVNQSGSYVDEERLRFDF
HHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
THFAALTEEELEKVELLVNEKIMTVSVVDTKEMSLDEARNSGATCLFDEKYAEKVRVVSV
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHCCCCCEEEECHHHHCCEEEEEE
GDFSKELCGGTHVANSGEIGLFKIVSESGVAAGIRRIEAVTGISALKFMELKNNMLKEAA
CCHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMLKCNEKDIAKRIAAQAHELKEKDKEIAELKAKLVQGAEDDILKDKVEINGVELVTAEL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCEEEEEEEH
KDVDGNSLRDLADKVRNKLNNGIVVLASDNGGKVNLVAMATKNSLANGVHCGKVIKEVAA
HCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEECCCHHHCCHHHHHHHHHHHH
VVGGGGGGRPDMAQAGGKNPENIAKALEKAKEVVELLVK
HHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA