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The map label for this gene is rnjA [H]

Identifier: 18310757

GI number: 18310757

Start: 2054313

End: 2056058

Strand: Reverse

Name: rnjA [H]

Synonym: CPE1775

Alternate gene names: 18310757

Gene position: 2056058-2054313 (Counterclockwise)

Preceding gene: 18310758

Following gene: 18310756

Centisome position: 67.82

GC content: 32.93

Gene sequence:

>1746_bases
ATGAATAGAGAAATTTTCTCAAATGAGGAAACCTCTTTTATACATAAGCGTACTACTACAAAAAAGAGGAGGGAAAATAT
GAAACGTGAAAAGCTAAAGGTTAAAATTATACCTTTAGGTGGTTTGAACGAGATAGGTAAGAATCTAACTGTTATCGAAT
TTAAAGACGATATAATTGTAGTCGACTGTGGACTTAAGTTCCCAGACGAAGATATGTTTGGTATTGATATAGTTATTCCA
GACGTATCATATCTTGTTAAAAATGCAGAAAAAGTTAGAGGAATATTTTTAACTCATGGTCATGAAGATCATATTGGTGC
TTTACCATATGTGTTAAAAAATTTAAATGTACCTGTTTATGGAACAAAACTTACTTTAGGAATAGTTGAAACTAAACTAA
AAGAACATGGTTTATTAAGCACCACAGAGCTTATAAGAGTTAAACCAAGAGATATTATAAAATTAAAATCATCATCTGTT
GAATTTGTAAAAACTAATCATAGTATAGCAGATTCAGTTGCTATAGCAGTGCATACACCACTAGGAGTTGTACTTCATAC
TGGTGATTTTAAAGTAGACTATACTCCAACTGACGGAGAGGTAATGGACTTTGCTAGATTTGCAGAATTAGGAAGAAAAG
GAGTTCTTGCAATGATGGCTGACTCTACTAATGTAGAGAGACCAGGATATACAATGTCAGAAAGAGCTGTAGGAGAAAAT
CTTAAAAAAATATTTGTTGGAGCAAAGGGAAGAATAATAATAGCTACATTTGCTTCAAATATTCATAGAATACAGCAAAT
TGTTGAAGCTGCAGAAATGACGGGAAGAAAGATTGCTGTTTCAGGAAGAAGTATGGAAAATATAGTTCAGGTTGCCATAG
AACTAGGATATTTAACAATAGATAAGGAGTCTTTTGTTAGCATAGATTCTATAAACAAATATCCAAATGAGCAAGTGACT
ATAATAACTACAGGAAGCCAAGGAGAGCCAATGTCAGCATTAGCTAGAATGGCATCTTCAGAGCATAAAAAAGTTAATAT
CATTGAGGGAGATACAGTAATACTATCTGCAACACCAATACCTGGTAATGAAAAGTTAGTTTCTAAGGTTATTAATCAAC
TTTTCAAAAAGGGAGCAGAAGTAGTGTATGGAAAACTAGCAGATGTCCATGTTTCAGGTCATGCTTGTCAAGAGGAATTA
AAACTTATGCAAGCTCTTGTTAAGCCTAAATTCTTTATACCAGTTCATGGTGAGTATAGACACCTTAAACAGCATGCAGA
GTTAGCTGTTGATGTTGGACTATCAGAAAAGAATTTTATGATAGCTGAAAATGGAGATGTTATAGAAATCACTAGAGATT
CAATCAAAAAGAATGGATCTGTTACTTCAGGACAAATTTTTGTTGATGGACTTGGAGTTGGTGATGTAGGAAACATAGTT
TTAAGAGATAGAAAACATCTTTCACAAGACGGAATTCTTACAGTAGTTGTTACTATTTCAAAAGAAACTGCTTCAGTGGT
AGCAGGACCAGATATTATATCAAGAGGTTTTGTTTACGTAAGAGAATCAGAAGACTTAATGGATGAAGCTAAAGAGATAG
TAAAAGACGTTTTAAGAGATTGTGAAAAGAAAGGAATCTGTGACTGGGCTACTATGAAATCTAACATAAGAGATGGCCTT
AGAAGTTTCCTTTATGAAAAAACTAAGAGAAAACCAATGATATTACCAATAATAATGGAAATATAA

Upstream 100 bases:

>100_bases
GATTTATTAGACGAACTAGAAGAAGAGATTGAAACTAAATATGGCTTTGAAATATTAGATCATACAGTAAAGTTTTTCGG
AATATGCAAGGATTGTAAAA

Downstream 100 bases:

>100_bases
AAAAATTAGATGATAATGTAGAATGTAACTGTTTAAGTAAAAAATGTTGTGTTGACTTTTTTAAAGCTTAACAGTAGAAT
CTAATAAGGTAAAGAAAATT

Product: hypothetical protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MNREIFSNEETSFIHKRTTTKKRRENMKREKLKVKIIPLGGLNEIGKNLTVIEFKDDIIVVDCGLKFPDEDMFGIDIVIP
DVSYLVKNAEKVRGIFLTHGHEDHIGALPYVLKNLNVPVYGTKLTLGIVETKLKEHGLLSTTELIRVKPRDIIKLKSSSV
EFVKTNHSIADSVAIAVHTPLGVVLHTGDFKVDYTPTDGEVMDFARFAELGRKGVLAMMADSTNVERPGYTMSERAVGEN
LKKIFVGAKGRIIIATFASNIHRIQQIVEAAEMTGRKIAVSGRSMENIVQVAIELGYLTIDKESFVSIDSINKYPNEQVT
IITTGSQGEPMSALARMASSEHKKVNIIEGDTVILSATPIPGNEKLVSKVINQLFKKGAEVVYGKLADVHVSGHACQEEL
KLMQALVKPKFFIPVHGEYRHLKQHAELAVDVGLSEKNFMIAENGDVIEITRDSIKKNGSVTSGQIFVDGLGVGDVGNIV
LRDRKHLSQDGILTVVVTISKETASVVAGPDIISRGFVYVRESEDLMDEAKEIVKDVLRDCEKKGICDWATMKSNIRDGL
RSFLYEKTKRKPMILPIIMEI

Sequences:

>Translated_581_residues
MNREIFSNEETSFIHKRTTTKKRRENMKREKLKVKIIPLGGLNEIGKNLTVIEFKDDIIVVDCGLKFPDEDMFGIDIVIP
DVSYLVKNAEKVRGIFLTHGHEDHIGALPYVLKNLNVPVYGTKLTLGIVETKLKEHGLLSTTELIRVKPRDIIKLKSSSV
EFVKTNHSIADSVAIAVHTPLGVVLHTGDFKVDYTPTDGEVMDFARFAELGRKGVLAMMADSTNVERPGYTMSERAVGEN
LKKIFVGAKGRIIIATFASNIHRIQQIVEAAEMTGRKIAVSGRSMENIVQVAIELGYLTIDKESFVSIDSINKYPNEQVT
IITTGSQGEPMSALARMASSEHKKVNIIEGDTVILSATPIPGNEKLVSKVINQLFKKGAEVVYGKLADVHVSGHACQEEL
KLMQALVKPKFFIPVHGEYRHLKQHAELAVDVGLSEKNFMIAENGDVIEITRDSIKKNGSVTSGQIFVDGLGVGDVGNIV
LRDRKHLSQDGILTVVVTISKETASVVAGPDIISRGFVYVRESEDLMDEAKEIVKDVLRDCEKKGICDWATMKSNIRDGL
RSFLYEKTKRKPMILPIIMEI
>Mature_581_residues
MNREIFSNEETSFIHKRTTTKKRRENMKREKLKVKIIPLGGLNEIGKNLTVIEFKDDIIVVDCGLKFPDEDMFGIDIVIP
DVSYLVKNAEKVRGIFLTHGHEDHIGALPYVLKNLNVPVYGTKLTLGIVETKLKEHGLLSTTELIRVKPRDIIKLKSSSV
EFVKTNHSIADSVAIAVHTPLGVVLHTGDFKVDYTPTDGEVMDFARFAELGRKGVLAMMADSTNVERPGYTMSERAVGEN
LKKIFVGAKGRIIIATFASNIHRIQQIVEAAEMTGRKIAVSGRSMENIVQVAIELGYLTIDKESFVSIDSINKYPNEQVT
IITTGSQGEPMSALARMASSEHKKVNIIEGDTVILSATPIPGNEKLVSKVINQLFKKGAEVVYGKLADVHVSGHACQEEL
KLMQALVKPKFFIPVHGEYRHLKQHAELAVDVGLSEKNFMIAENGDVIEITRDSIKKNGSVTSGQIFVDGLGVGDVGNIV
LRDRKHLSQDGILTVVVTISKETASVVAGPDIISRGFVYVRESEDLMDEAKEIVKDVLRDCEKKGICDWATMKSNIRDGL
RSFLYEKTKRKPMILPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 64401; Mature: 64401

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREIFSNEETSFIHKRTTTKKRRENMKREKLKVKIIPLGGLNEIGKNLTVIEFKDDIIV
CCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCEEEEEEECCCHHHHCCCEEEEEEECCEEE
VDCGLKFPDEDMFGIDIVIPDVSYLVKNAEKVRGIFLTHGHEDHIGALPYVLKNLNVPVY
EECCCCCCCCCCEEEEEEECCHHHHHHCHHHHCEEEEECCCCCCCCHHHHHHHHCCCCEE
GTKLTLGIVETKLKEHGLLSTTELIRVKPRDIIKLKSSSVEFVKTNHSIADSVAIAVHTP
ECEEEEHHHHHHHHHCCCCCCCCEEEECCHHHEEECCCCEEEEECCCCHHCCEEEEEECC
LGVVLHTGDFKVDYTPTDGEVMDFARFAELGRKGVLAMMADSTNVERPGYTMSERAVGEN
CEEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
LKKIFVGAKGRIIIATFASNIHRIQQIVEAAEMTGRKIAVSGRSMENIVQVAIELGYLTI
HHHHEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCEEEE
DKESFVSIDSINKYPNEQVTIITTGSQGEPMSALARMASSEHKKVNIIEGDTVILSATPI
CHHHCEEHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCEEEEEECCC
PGNEKLVSKVINQLFKKGAEVVYGKLADVHVSGHACQEELKLMQALVKPKFFIPVHGEYR
CCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHCCEEEEECCCHHH
HLKQHAELAVDVGLSEKNFMIAENGDVIEITRDSIKKNGSVTSGQIFVDGLGVGDVGNIV
HHHHHHHEEEEECCCCCCEEEECCCCEEEEEHHHHHCCCCEECCEEEEEECCCCCCCCEE
LRDRKHLSQDGILTVVVTISKETASVVAGPDIISRGFVYVRESEDLMDEAKEIVKDVLRD
EECCHHCCCCCEEEEEEEECCCCHHEEECCHHHHCCEEEEECCHHHHHHHHHHHHHHHHH
CEKKGICDWATMKSNIRDGLRSFLYEKTKRKPMILPIIMEI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MNREIFSNEETSFIHKRTTTKKRRENMKREKLKVKIIPLGGLNEIGKNLTVIEFKDDIIV
CCCCCCCCCCHHHHHHHHHHHHHHHCHHHHCEEEEEEECCCHHHHCCCEEEEEEECCEEE
VDCGLKFPDEDMFGIDIVIPDVSYLVKNAEKVRGIFLTHGHEDHIGALPYVLKNLNVPVY
EECCCCCCCCCCEEEEEEECCHHHHHHCHHHHCEEEEECCCCCCCCHHHHHHHHCCCCEE
GTKLTLGIVETKLKEHGLLSTTELIRVKPRDIIKLKSSSVEFVKTNHSIADSVAIAVHTP
ECEEEEHHHHHHHHHCCCCCCCCEEEECCHHHEEECCCCEEEEECCCCHHCCEEEEEECC
LGVVLHTGDFKVDYTPTDGEVMDFARFAELGRKGVLAMMADSTNVERPGYTMSERAVGEN
CEEEEECCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
LKKIFVGAKGRIIIATFASNIHRIQQIVEAAEMTGRKIAVSGRSMENIVQVAIELGYLTI
HHHHEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCEEEE
DKESFVSIDSINKYPNEQVTIITTGSQGEPMSALARMASSEHKKVNIIEGDTVILSATPI
CHHHCEEHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCEEEEEECCC
PGNEKLVSKVINQLFKKGAEVVYGKLADVHVSGHACQEELKLMQALVKPKFFIPVHGEYR
CCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHCCEEEEECCCHHH
HLKQHAELAVDVGLSEKNFMIAENGDVIEITRDSIKKNGSVTSGQIFVDGLGVGDVGNIV
HHHHHHHEEEEECCCCCCEEEECCCCEEEEEHHHHHCCCCEECCEEEEEECCCCCCCCEE
LRDRKHLSQDGILTVVVTISKETASVVAGPDIISRGFVYVRESEDLMDEAKEIVKDVLRD
EECCHHCCCCCEEEEEEEECCCCHHEEECCHHHHCCEEEEECCHHHHHHHHHHHHHHHHH
CEKKGICDWATMKSNIRDGLRSFLYEKTKRKPMILPIIMEI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]