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The map label for this gene is ansA [H]

Identifier: 18310734

GI number: 18310734

Start: 2028956

End: 2029939

Strand: Reverse

Name: ansA [H]

Synonym: CPE1752

Alternate gene names: 18310734

Gene position: 2029939-2028956 (Counterclockwise)

Preceding gene: 18310735

Following gene: 18310733

Centisome position: 66.96

GC content: 32.52

Gene sequence:

>984_bases
ATGAAGAAAATAGCTGTAATTTTTAATGGTGGAACAATATCAATGAAAGTTGATGATAGAATAAAGGCTGCAGTTCCAAG
TTTGACAGGAGAGGAAATAATGGCTATGGTAACAGGAATAGAAAGCTTTGCAACAATAGAGTCACATAGCTTTTCTTCAT
TACCAAGTCCACATATTACTCCAGAGATAATGCTTGAACTATCAAAATTTATAAATGCTTTAGTAGAGAGAGAAGATATA
GATGGAGTTGTAGTAACTCACGGAACAGATACTCTAGAAGAAACATCTTATTTTGTTAATTTAACAACTAAGACAAGTAA
ACCAATAGTGTTTACTGGAGCTATGAGAAGTGGATCAGAGTTAGGATATGATGGACCAGCTAACTTAGCTGCATCAATAT
GTACAGCTGCATCAGATGAAGCAAAAAATAGAGGAGTTCTAGTTTGTTTTAATGGGGAATTAAATAGTGCTTCAGAGGTA
ACTAAGGCTAATTCAATGGCTTTAAATGCTTTTAGAACACCTAATTTTGGCCCAATAGGAATAGTTGATAATAATAGGGT
AATATTTGATAGAAGAGTTCCACAGGAAGATTTTATACCTTTAGAGGTTATTGATAAAAAAGTAGCCATAATAAAATGTG
CTGCAGGTATGGATGGAGATTTTATATATCACTGTATAGAGAAAAAATATGATGGAATCATAATAGAAGCTTTAGGAAGA
GGAAATGTACCACCAACTATGGTTGATGCAATAAAAGATGCCTTAGATAAGGGAATAGTTATAGTACTTACTTCAAGATG
TTTTGAAGGAAGAGTAAGTGATAGCTATGGATATCTTGGAGGTGGAAAAAATCTTAAAGAGCTCGGGGTTATATTTGGAG
AAAGTATGCCAAGTCAAAAGGCTAGAATAAAGCTTTTAACTCTTCTTGGATTTAATAAAGATTATAGATATATTAAACAT
AGTTTTGAGAAAGAAAGATATTAA

Upstream 100 bases:

>100_bases
GTTTAGCTTTGCAAAGATTGAATAGAATTATTAATTTAATTATAATAAGCATTGTGACGAATGATACTTAGTCAAATAAA
AAAGAAATTCGGAGGTACTC

Downstream 100 bases:

>100_bases
ACACATATATTTTAAAAATAATAATAAAAAATATTCGATAATTATTGAATAATAACTTAGAATAGTGTACAATAACATTC
GTAGACAGATGAAAAGTTCA

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKKIAVIFNGGTISMKVDDRIKAAVPSLTGEEIMAMVTGIESFATIESHSFSSLPSPHITPEIMLELSKFINALVEREDI
DGVVVTHGTDTLEETSYFVNLTTKTSKPIVFTGAMRSGSELGYDGPANLAASICTAASDEAKNRGVLVCFNGELNSASEV
TKANSMALNAFRTPNFGPIGIVDNNRVIFDRRVPQEDFIPLEVIDKKVAIIKCAAGMDGDFIYHCIEKKYDGIIIEALGR
GNVPPTMVDAIKDALDKGIVIVLTSRCFEGRVSDSYGYLGGGKNLKELGVIFGESMPSQKARIKLLTLLGFNKDYRYIKH
SFEKERY

Sequences:

>Translated_327_residues
MKKIAVIFNGGTISMKVDDRIKAAVPSLTGEEIMAMVTGIESFATIESHSFSSLPSPHITPEIMLELSKFINALVEREDI
DGVVVTHGTDTLEETSYFVNLTTKTSKPIVFTGAMRSGSELGYDGPANLAASICTAASDEAKNRGVLVCFNGELNSASEV
TKANSMALNAFRTPNFGPIGIVDNNRVIFDRRVPQEDFIPLEVIDKKVAIIKCAAGMDGDFIYHCIEKKYDGIIIEALGR
GNVPPTMVDAIKDALDKGIVIVLTSRCFEGRVSDSYGYLGGGKNLKELGVIFGESMPSQKARIKLLTLLGFNKDYRYIKH
SFEKERY
>Mature_327_residues
MKKIAVIFNGGTISMKVDDRIKAAVPSLTGEEIMAMVTGIESFATIESHSFSSLPSPHITPEIMLELSKFINALVEREDI
DGVVVTHGTDTLEETSYFVNLTTKTSKPIVFTGAMRSGSELGYDGPANLAASICTAASDEAKNRGVLVCFNGELNSASEV
TKANSMALNAFRTPNFGPIGIVDNNRVIFDRRVPQEDFIPLEVIDKKVAIIKCAAGMDGDFIYHCIEKKYDGIIIEALGR
GNVPPTMVDAIKDALDKGIVIVLTSRCFEGRVSDSYGYLGGGKNLKELGVIFGESMPSQKARIKLLTLLGFNKDYRYIKH
SFEKERY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=240, Percent_Identity=25.4166666666667, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1789327, Length=308, Percent_Identity=30.8441558441558, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788065, Length=337, Percent_Identity=26.4094955489614, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17558278, Length=341, Percent_Identity=26.9794721407625, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17558280, Length=341, Percent_Identity=26.9794721407625, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI193207372, Length=341, Percent_Identity=26.9794721407625, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI193207374, Length=341, Percent_Identity=26.9794721407625, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320527, Length=338, Percent_Identity=28.9940828402367, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323189, Length=191, Percent_Identity=31.9371727748691, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323187, Length=191, Percent_Identity=31.9371727748691, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323186, Length=191, Percent_Identity=31.9371727748691, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323184, Length=191, Percent_Identity=31.9371727748691, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24645530, Length=238, Percent_Identity=30.672268907563, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639609, Length=350, Percent_Identity=25.1428571428571, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35680; Mature: 35680

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAVIFNGGTISMKVDDRIKAAVPSLTGEEIMAMVTGIESFATIESHSFSSLPSPHIT
CCEEEEEEECCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
PEIMLELSKFINALVEREDIDGVVVTHGTDTLEETSYFVNLTTKTSKPIVFTGAMRSGSE
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCC
LGYDGPANLAASICTAASDEAKNRGVLVCFNGELNSASEVTKANSMALNAFRTPNFGPIG
CCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHEEEEEECCCCCCEEE
IVDNNRVIFDRRVPQEDFIPLEVIDKKVAIIKCAAGMDGDFIYHCIEKKYDGIIIEALGR
EEECCEEEEECCCCCCCCCCHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCC
GNVPPTMVDAIKDALDKGIVIVLTSRCFEGRVSDSYGYLGGGKNLKELGVIFGESMPSQK
CCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
ARIKLLTLLGFNKDYRYIKHSFEKERY
CEEEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIAVIFNGGTISMKVDDRIKAAVPSLTGEEIMAMVTGIESFATIESHSFSSLPSPHIT
CCEEEEEEECCEEEEEECCCHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCC
PEIMLELSKFINALVEREDIDGVVVTHGTDTLEETSYFVNLTTKTSKPIVFTGAMRSGSE
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEEECCCCCCC
LGYDGPANLAASICTAASDEAKNRGVLVCFNGELNSASEVTKANSMALNAFRTPNFGPIG
CCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHEEEEEECCCCCCEEE
IVDNNRVIFDRRVPQEDFIPLEVIDKKVAIIKCAAGMDGDFIYHCIEKKYDGIIIEALGR
EEECCEEEEECCCCCCCCCCHHHHCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEECCC
GNVPPTMVDAIKDALDKGIVIVLTSRCFEGRVSDSYGYLGGGKNLKELGVIFGESMPSQK
CCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
ARIKLLTLLGFNKDYRYIKHSFEKERY
CEEEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]