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Identifier: 18310726

GI number: 18310726

Start: 2021197

End: 2021640

Strand: Reverse

Name: def

Synonym: CPE1744

Alternate gene names: 18310726

Gene position: 2021640-2021197 (Counterclockwise)

Preceding gene: 18310727

Following gene: 18310725

Centisome position: 66.69

GC content: 31.08

Gene sequence:

>444_bases
TTGGCAATAAGAAACTTAAGATTTAATGACGACGAAATATTAAGAAAAAAATGTAGAGTAGTTGATGACATTAATGATAG
AATAAAAGTCTTAGTAGAAGATATGATAGAAACTATGTATGAAAATAATGGAGTTGGATTAGCATCTCCACAAGTTGGAA
TACTAAAGAGAATATTTGTTGTTGATGCTATGGATGGAGCGGGTTCTAGAGTATTCATAAATCCAGAAATTTTAGAAAAA
AGTGGAGAGCAAACTGATGAAGAAGGATGCTTAAGCTTACCAGGTAGACATAAACCAGTAAAGAGAGCTAATAAAATAAA
GATAAAGGCTTTAGATGTTAATGGAAATGAGTTTGTATTAGATGCAGAAGGATTTTTAGCAAGGGCTATACAACATGAAT
ATGATCATTTAGAGGGAGTACTTTTTATAGATCATGAGCTTTAA

Upstream 100 bases:

>100_bases
ATAAAAAAAGGGGTTTATGAATTATCCAAAGATGTTTATAATGAGATTAAGATTGGTTTAGATATAAATCCTAATAGCTT
AATGTAAGGAGGAAGTTATT

Downstream 100 bases:

>100_bases
GACTAAGGAGGGATATGACATGAAGATAGTATTTATGGGAACACCAGATTTTGCTGTTCCATCATTAAAGAGCTTAATAA
ATGAATTTGGAGTAGAAGCT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAIRNLRFNDDEILRKKCRVVDDINDRIKVLVEDMIETMYENNGVGLASPQVGILKRIFVVDAMDGAGSRVFINPEILEK
SGEQTDEEGCLSLPGRHKPVKRANKIKIKALDVNGNEFVLDAEGFLARAIQHEYDHLEGVLFIDHEL

Sequences:

>Translated_147_residues
MAIRNLRFNDDEILRKKCRVVDDINDRIKVLVEDMIETMYENNGVGLASPQVGILKRIFVVDAMDGAGSRVFINPEILEK
SGEQTDEEGCLSLPGRHKPVKRANKIKIKALDVNGNEFVLDAEGFLARAIQHEYDHLEGVLFIDHEL
>Mature_146_residues
AIRNLRFNDDEILRKKCRVVDDINDRIKVLVEDMIETMYENNGVGLASPQVGILKRIFVVDAMDGAGSRVFINPEILEKS
GEQTDEEGCLSLPGRHKPVKRANKIKIKALDVNGNEFVLDAEGFLARAIQHEYDHLEGVLFIDHEL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=135, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1789682, Length=146, Percent_Identity=50, Blast_Score=137, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645728, Length=156, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=31.4102564102564, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF1_CLOPE (Q8XJL2)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562660.1
- ProteinModelPortal:   Q8XJL2
- SMR:   Q8XJL2
- GeneID:   990054
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1744
- NMPDR:   fig|195102.1.peg.1807
- HOGENOM:   HBG665227
- OMA:   AADMAET
- PhylomeDB:   Q8XJL2
- ProtClustDB:   PRK00150
- BioCyc:   CPER195102:CPE1744-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 16616; Mature: 16485

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: ACT_SITE 133-133

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRNLRFNDDEILRKKCRVVDDINDRIKVLVEDMIETMYENNGVGLASPQVGILKRIFV
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHE
VDAMDGAGSRVFINPEILEKSGEQTDEEGCLSLPGRHKPVKRANKIKIKALDVNGNEFVL
EECCCCCCCEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEE
DAEGFLARAIQHEYDHLEGVLFIDHEL
ECHHHHHHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
AIRNLRFNDDEILRKKCRVVDDINDRIKVLVEDMIETMYENNGVGLASPQVGILKRIFV
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHE
VDAMDGAGSRVFINPEILEKSGEQTDEEGCLSLPGRHKPVKRANKIKIKALDVNGNEFVL
EECCCCCCCEEEECHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEE
DAEGFLARAIQHEYDHLEGVLFIDHEL
ECHHHHHHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842