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The map label for this gene is fmt

Identifier: 18310725

GI number: 18310725

Start: 2020248

End: 2021177

Strand: Reverse

Name: fmt

Synonym: CPE1743

Alternate gene names: 18310725

Gene position: 2021177-2020248 (Counterclockwise)

Preceding gene: 18310726

Following gene: 18310724

Centisome position: 66.67

GC content: 31.18

Gene sequence:

>930_bases
ATGAAGATAGTATTTATGGGAACACCAGATTTTGCTGTTCCATCATTAAAGAGCTTAATAAATGAATTTGGAGTAGAAGC
TGTTTTTACTCAACCAGATAGACCGAAGGGAAGAGGAAAGAAATTAGGTATGTCACCTGTAAAGGAAGTTGCTTTAGAAC
ACAACATACCAGTTTATCAACCTTTAAGATTAAAAAATGAACCAGAAACTATAGAAGAACTAAAAAATATGGAGCCAGAC
TTTATAATAGTAGTAGCTTTTGGTCAGATATTACCTAAAGAGGTTTTAGATATTCCTAAGTATGGGTGTATAAATTTACA
TGCTTCATTACTTCCTAAATTTAGAGGAGCAGCACCTTTAAATTGGAGTATAATCAAAGGAGAAAAGGTAACAGGAAATA
CAACTATGCTTATGGATGTAGGACTTGATACTGGTGATATGCTTTTAAAAGATGAAGTAGAAATAACTGATAATATGACT
GCAGGAGAATTACATGATATTTTAATGGAAAGAGGAGGAGAGTTATTAGTTAGAACTATAAAGGGCATACTTAATAATGA
AATAACTCCAGAAAAGCAAAATGAAGAAGAAACATGTTATGCTCCAATGCTTAATAAAGAAATAGCTAAAATTGATTGGT
CTCTTTCAGCACAGGATATTCATAATTTAGTTAGAGGATTAAATCCGTGGCCTGTAGCTTTAACTTCATATGATGATATT
ACAATGAAAGTTCATCAAACTAGAGTTGAAAAAGGGGATAGCAATAAAGAGCCAGGAACTATAATATCTGTAGATAAAAC
AGGAATTAAGGTTTCAACAGGAAAAGATATACTTGTTATAGAAAAATTACAGTTTCCAAATAGCAAGCAACTATTTGTAG
AGCAATTTATAAATGGAAACTCAGTAGAAATAGGTAAAGTTTTAAAATAA

Upstream 100 bases:

>100_bases
GCAGAAGGATTTTTAGCAAGGGCTATACAACATGAATATGATCATTTAGAGGGAGTACTTTTTATAGATCATGAGCTTTA
AGACTAAGGAGGGATATGAC

Downstream 100 bases:

>100_bases
TTTAAGAGGGTGATGGTATGTTTTATCCATATTATATTGATCCAACATATTTAATACTTATACCAGCGATTTTAATATCT
GCATGGGCACAATTTAAAGT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKIVFMGTPDFAVPSLKSLINEFGVEAVFTQPDRPKGRGKKLGMSPVKEVALEHNIPVYQPLRLKNEPETIEELKNMEPD
FIIVVAFGQILPKEVLDIPKYGCINLHASLLPKFRGAAPLNWSIIKGEKVTGNTTMLMDVGLDTGDMLLKDEVEITDNMT
AGELHDILMERGGELLVRTIKGILNNEITPEKQNEEETCYAPMLNKEIAKIDWSLSAQDIHNLVRGLNPWPVALTSYDDI
TMKVHQTRVEKGDSNKEPGTIISVDKTGIKVSTGKDILVIEKLQFPNSKQLFVEQFINGNSVEIGKVLK

Sequences:

>Translated_309_residues
MKIVFMGTPDFAVPSLKSLINEFGVEAVFTQPDRPKGRGKKLGMSPVKEVALEHNIPVYQPLRLKNEPETIEELKNMEPD
FIIVVAFGQILPKEVLDIPKYGCINLHASLLPKFRGAAPLNWSIIKGEKVTGNTTMLMDVGLDTGDMLLKDEVEITDNMT
AGELHDILMERGGELLVRTIKGILNNEITPEKQNEEETCYAPMLNKEIAKIDWSLSAQDIHNLVRGLNPWPVALTSYDDI
TMKVHQTRVEKGDSNKEPGTIISVDKTGIKVSTGKDILVIEKLQFPNSKQLFVEQFINGNSVEIGKVLK
>Mature_309_residues
MKIVFMGTPDFAVPSLKSLINEFGVEAVFTQPDRPKGRGKKLGMSPVKEVALEHNIPVYQPLRLKNEPETIEELKNMEPD
FIIVVAFGQILPKEVLDIPKYGCINLHASLLPKFRGAAPLNWSIIKGEKVTGNTTMLMDVGLDTGDMLLKDEVEITDNMT
AGELHDILMERGGELLVRTIKGILNNEITPEKQNEEETCYAPMLNKEIAKIDWSLSAQDIHNLVRGLNPWPVALTSYDDI
TMKVHQTRVEKGDSNKEPGTIISVDKTGIKVSTGKDILVIEKLQFPNSKQLFVEQFINGNSVEIGKVLK

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=319, Percent_Identity=28.5266457680251, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI238814322, Length=308, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI164663775, Length=237, Percent_Identity=27.0042194092827, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789683, Length=299, Percent_Identity=45.1505016722408, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1788589, Length=277, Percent_Identity=31.7689530685921, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI133930964, Length=247, Percent_Identity=30.3643724696356, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45550868, Length=308, Percent_Identity=30.5194805194805, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=33.3333333333333, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24585660, Length=244, Percent_Identity=28.2786885245902, Blast_Score=94, Evalue=9e-20,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_CLOPE (Q8XJL3)

Other databases:

- EMBL:   BA000016
- RefSeq:   NP_562659.1
- ProteinModelPortal:   Q8XJL3
- SMR:   Q8XJL3
- GeneID:   990053
- GenomeReviews:   BA000016_GR
- KEGG:   cpe:CPE1743
- NMPDR:   fig|195102.1.peg.1806
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q8XJL3
- ProtClustDB:   PRK00005
- BioCyc:   CPER195102:CPE1743-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34429; Mature: 34429

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFMGTPDFAVPSLKSLINEFGVEAVFTQPDRPKGRGKKLGMSPVKEVALEHNIPVYQ
CEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
PLRLKNEPETIEELKNMEPDFIIVVAFGQILPKEVLDIPKYGCINLHASLLPKFRGAAPL
CCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEECHHHHHHHHCCCCCC
NWSIIKGEKVTGNTTMLMDVGLDTGDMLLKDEVEITDNMTAGELHDILMERGGELLVRTI
CEEEEECCEECCCEEEEEEECCCCCCEEEECCEEECCCCCHHHHHHHHHHCCCHHHHHHH
KGILNNEITPEKQNEEETCYAPMLNKEIAKIDWSLSAQDIHNLVRGLNPWPVALTSYDDI
HHHHCCCCCCCCCCCCCHHHHHHHCCHHHHEECCCCHHHHHHHHHCCCCCCEEEECCCCE
TMKVHQTRVEKGDSNKEPGTIISVDKTGIKVSTGKDILVIEKLQFPNSKQLFVEQFINGN
EEEEHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCEEEEEEECCCCCHHHHHHHHCCCC
SVEIGKVLK
CEECCCCCC
>Mature Secondary Structure
MKIVFMGTPDFAVPSLKSLINEFGVEAVFTQPDRPKGRGKKLGMSPVKEVALEHNIPVYQ
CEEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCC
PLRLKNEPETIEELKNMEPDFIIVVAFGQILPKEVLDIPKYGCINLHASLLPKFRGAAPL
CCCCCCCCHHHHHHHCCCCCEEEEEECCHHHHHHHHCCCCCCEEECHHHHHHHHCCCCCC
NWSIIKGEKVTGNTTMLMDVGLDTGDMLLKDEVEITDNMTAGELHDILMERGGELLVRTI
CEEEEECCEECCCEEEEEEECCCCCCEEEECCEEECCCCCHHHHHHHHHHCCCHHHHHHH
KGILNNEITPEKQNEEETCYAPMLNKEIAKIDWSLSAQDIHNLVRGLNPWPVALTSYDDI
HHHHCCCCCCCCCCCCCHHHHHHHCCHHHHEECCCCHHHHHHHHHCCCCCCEEEECCCCE
TMKVHQTRVEKGDSNKEPGTIISVDKTGIKVSTGKDILVIEKLQFPNSKQLFVEQFINGN
EEEEHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCEEEEEEECCCCCHHHHHHHHCCCC
SVEIGKVLK
CEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11792842